Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 696,177 T→C N359N (AAT→AAC)  ubiF → 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913696,1770TC99.4% 600.0 / ‑4.7 171N359N (AAT→AAC) ubiF2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (96/74);  total (97/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

Only 100 of 174 total aligned reads displayed.

TTATCCTGTCCTCAAGCGTTACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTG  >  NC_000913/696081‑696273
                                                                                                |                                                                                                
 tATCCTGTCCTCAAGCGTTACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGc                                                                                              >  1:3427948/1‑100 (MQ=255)
   tCCTGTCCTCAAGCGTTACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGcca                                                                                            <  1:2532015/100‑1 (MQ=255)
    ccTGTCCTCAAGCGTTACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGccac                                                                                           <  1:2719189/100‑1 (MQ=255)
     gcgTCCTCAAGCGTTACCAGATGCGGCGCATGGCGGCTCACTTTATTATGCGGAGCGGTAAGGATCTGGTTTATGCCGGCTTTAGCCATAACCTGccacc                                                                                          >  2:3415634/3‑100 (MQ=255)
     cTGTCCTCAAGCGTTACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGccacc                                                                                          <  2:913828/100‑1 (MQ=255)
      tGTCCTCAAGCGTTACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGccacca                                                                                         >  2:2238700/1‑100 (MQ=255)
         ccTCAAGCGTTACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTg                                                                                      <  1:275012/100‑1 (MQ=255)
          cTCAAGCGTTACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGc                                                                                     >  2:4057319/1‑100 (MQ=255)
          cTCAAGCGTTACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGc                                                                                     >  2:1738867/1‑100 (MQ=255)
          cTCAAGCGTTACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGCTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACtcc                                                                                     >  2:2673756/1‑98 (MQ=255)
             aaGCGTTACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGtt                                                                                  <  2:2531607/100‑1 (MQ=255)
              aGCGTTACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGttt                                                                                 <  1:2768387/100‑1 (MQ=255)
                 gTTACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTAt                                                                              >  2:2219158/1‑100 (MQ=255)
                  ttACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTCTGCCGGATTCAGCAATAGCCTGCCACCACTGCGTTTTATg                                                                             <  2:4197185/100‑1 (MQ=255)
                  ttACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATg                                                                             >  2:3410140/1‑100 (MQ=255)
                  ttACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATg                                                                             <  1:2734013/100‑1 (MQ=255)
                  ttACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATg                                                                             <  1:64294/100‑1 (MQ=255)
                  ttACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATg                                                                             <  2:1038434/100‑1 (MQ=255)
                   tACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAGAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCAGTGCGTTTTATGc                                                                            <  2:4889396/100‑1 (MQ=255)
                   tACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGc                                                                            >  1:2220908/1‑100 (MQ=255)
                    aCCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCg                                                                           >  1:1796377/1‑100 (MQ=255)
                     ccAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGt                                                                          >  2:1489767/1‑100 (MQ=255)
                     ccAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGt                                                                          >  2:1092433/1‑100 (MQ=255)
                      cAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTa                                                                         <  2:303899/100‑1 (MQ=255)
                      cAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTa                                                                         <  2:4218380/100‑1 (MQ=255)
                        gATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAAt                                                                       <  1:5154458/100‑1 (MQ=255)
                            cggcgCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCg                                                                   >  1:1629681/1‑100 (MQ=255)
                             ggcgCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCgg                                                                  >  1:3493439/1‑100 (MQ=255)
                              gcgcATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCggn                                                                 <  1:1295817/100‑2 (MQ=255)
                              gcgcATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCggg                                                                 <  2:2151471/100‑1 (MQ=255)
                                 cATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTa                                                              <  2:2894504/100‑1 (MQ=255)
                                  aTGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTaa                                                             >  1:727100/1‑100 (MQ=255)
                                   tGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGTGTAATCTCGGGTTAAt                                                            >  2:3511276/1‑100 (MQ=255)
                                     gCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATgg                                                          >  1:3999709/1‑100 (MQ=255)
                                      cGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCGCTGCGTTTTATGCGTAATCTCGGGTTAATggc                                                         <  1:5081406/100‑1 (MQ=255)
                                      cGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATggc                                                         <  2:1793949/100‑1 (MQ=255)
                                      cGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATggc                                                         >  1:3629351/1‑100 (MQ=255)
                                      cGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATggc                                                         >  1:4293974/1‑100 (MQ=255)
                                         aTAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATggcggc                                                      <  2:4345879/100‑1 (MQ=255)
                                          tAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGgcggcg                                                     >  1:2644462/1‑100 (MQ=255)
                                            aCTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATCACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGa                                                   >  1:2329541/1‑100 (MQ=255)
                                             cTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAg                                                  >  2:3164119/1‑100 (MQ=255)
                                             cTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAg                                                  >  2:4552177/1‑100 (MQ=255)
                                             cTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAg                                                  >  2:26879/1‑100 (MQ=255)
                                             cTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAg                                                  <  1:4579478/100‑1 (MQ=255)
                                               tCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCg                                                <  1:2595907/100‑1 (MQ=255)
                                                cATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGt                                               >  1:4267409/1‑100 (MQ=255)
                                                cATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGt                                               >  1:3317039/1‑100 (MQ=255)
                                                 aTTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTg                                              >  2:2747540/1‑100 (MQ=255)
                                                  ttATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCCCTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGc                                             <  2:789327/100‑1 (MQ=255)
                                                  ttATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGc                                             <  2:1430470/100‑1 (MQ=255)
                                                    aTGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTg                                           >  1:152818/1‑100 (MQ=255)
                                                    aTGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTg                                           <  2:2058793/100‑1 (MQ=255)
                                                     tGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTgg                                          >  1:2995614/1‑100 (MQ=255)
                                                      gCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGc                                         >  1:4870156/1‑100 (MQ=255)
                                                       cAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTATTGGCGGCGGAGCGTGCTcgcg                                        >  2:1196821/1‑100 (MQ=255)
                                                       cAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCg                                        <  2:4301553/100‑1 (MQ=255)
                                                       cAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCg                                        <  2:4294342/100‑1 (MQ=255)
                                                       cAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCg                                        >  1:3188552/1‑100 (MQ=255)
                                                         aaGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCgtg                                      <  1:4619360/100‑1 (MQ=255)
                                                          aGCGGTATGGATCTGTTTTATGCCGGCTTCAGCAATAACCTGCCAGCACTGCGTTTTATGCGTAATCTCGCGTTAGTGGCGGCGGAGCGTGCTGGCgtgt                                     <  1:4815996/100‑1 (MQ=255)
                                                           gCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGtt                                    <  1:800890/100‑1 (MQ=255)
                                                           gCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGGGtt                                    <  1:862735/100‑1 (MQ=255)
                                                           gCGGTATGGAGCTGTTTGATGCCGGAGTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGtt                                    <  1:1149534/100‑1 (MQ=255)
                                                            cGGTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTg                                   <  2:1607334/100‑1 (MQ=255)
                                                             ngTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGa                                  >  1:4094159/2‑100 (MQ=255)
                                                             ggTATGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGa                                  >  2:2097519/1‑100 (MQ=255)
                                                               tATGGACCTGTTGTATGCCGGAGTCAGCAAGAACCTGCCACCACTCCGTTCTATGCGTAGTCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTTTCGaaa                                <  2:4785638/100‑1 (MQ=255)
                                                                aTGGATCTGTTTTAGGCCGGATTCTGCAATAACCTTCCACCACTGCGTTTTATGTGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAAc                               <  2:4563727/100‑1 (MQ=255)
                                                                 tGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGTGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACg                              <  2:1350253/100‑1 (MQ=255)
                                                                 tGGATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACg                              <  2:4402773/100‑1 (MQ=255)
                                                                  ggATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGt                             >  1:3932414/1‑100 (MQ=255)
                                                                   gATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTc                            <  2:4075280/100‑1 (MQ=255)
                                                                   gATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTc                            >  2:3276879/1‑100 (MQ=255)
                                                                   gATCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTc                            <  2:1976549/100‑1 (MQ=255)
                                                                    aTCTGTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCa                           >  2:3561753/1‑100 (MQ=255)
                                                                        gTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAggag                       >  1:438464/1‑98 (MQ=255)
                                                                        gTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGgcg                       >  1:3678932/1‑100 (MQ=255)
                                                                        gTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGgcg                       >  2:3655039/1‑100 (MQ=255)
                                                                        gTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGgcg                       >  1:327237/1‑100 (MQ=255)
                                                                        gTTTTATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTAGCGTGTTGAAACGTCAGgcg                       >  1:5156480/1‑100 (MQ=255)
                                                                          tttATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCt                     >  2:1536248/1‑100 (MQ=255)
                                                                          tttATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCt                     <  1:1490237/100‑1 (MQ=255)
                                                                           ttATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTg                    <  2:3853711/100‑1 (MQ=255)
                                                                            tATGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGTGTGTTGAAACGTCAGGCGCTGa                   <  2:1575247/100‑1 (MQ=255)
                                                                             aTGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGaa                  >  2:629104/1‑100 (MQ=255)
                                                                             aTGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGaa                  <  1:47111/100‑1 (MQ=255)
                                                                              tGCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGaaa                 >  1:561165/1‑100 (MQ=255)
                                                                               gCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAat                >  1:3230937/1‑100 (MQ=255)
                                                                               gCCGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAat                >  1:2119291/1‑100 (MQ=255)
                                                                                ccGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAata               >  1:4209646/1‑100 (MQ=255)
                                                                                 cGGATTCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAatat              >  1:1528611/1‑100 (MQ=255)
                                                                                      tCAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGtt         <  1:3448173/100‑1 (MQ=255)
                                                                                       cAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTa        >  2:4284567/1‑100 (MQ=255)
                                                                                       cAGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTa        >  2:3678170/1‑100 (MQ=255)
                                                                                        ngCAATAACCTGCCACCACTGCGTTTTATGCGTAATATCGGGTTAATGGCGGCGGCGCGTGATGGCGTGTTGAAACGTCAGGCGACGGAATATTCGttca       >  1:4108725/2‑98 (MQ=255)
                                                                                        aGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAg       <  2:2421227/100‑1 (MQ=255)
                                                                                        aGCAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAg       >  1:2557559/1‑100 (MQ=255)
                                                                                          cAATAACCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAggg     >  2:1271125/1‑100 (MQ=255)
                                                                                             taacCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTg  >  2:581398/1‑100 (MQ=255)
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TTATCCTGTCCTCAAGCGTTACCAGATGCGGCGCATGGCGGATAACTTCATTATGCAAAGCGGTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTG  >  NC_000913/696081‑696273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑