| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 417,533 | 0 | T | G | 50.0% | 26.6 / 39.5 | 86 | V131G (GTG→GGG) | phoB | response regulator in two‑component regulatory system with PhoR |
| Reads supporting (aligned to +/- strand): ref base T (31/12); new base G (42/1); total (73/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.60e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGA‑ATTTAAACTGCTG‑CAC‑TTTTTTATGACGCATCCTGAGCGCGTGTAC‑AGCCGCGAGCAG‑CTGTTAAACCACGTCTGGGGAACTAACGTGTAT‑GTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGG > NC_000913/417295‑417770 | ctcGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGt > 1:1594402/1‑239 (MQ=255)aggccgggggtGTTACCGGGCGCCCCCGGTATCCAGTGCATCAACCCCCTCAAGCGCGAGTCGATGACCCGGGAGATTCCAGTGGTGATGTTGACCGGCGGAGGGGAAGAAAAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGt < 2:677847/229‑1 (MQ=255) gATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTgaga > 1:679020/1‑239 (MQ=255) aCCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGGGATGCAgg > 1:3044368/1‑239 (MQ=255) ccTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGGGGGGATTGGGTTGCAagg > 2:588427/1‑239 (MQ=255) ccTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGGTTGAGCTGCAggg > 1:505882/1‑239 (MQ=255) ccTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGTGGAGAGGGGGTTGGGAAGGAggg > 2:2461427/1‑239 (MQ=255) ggcggcTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGGTTGGGGTGGCGGGAtt > 1:82783/1‑239 (MQ=255) ggcggcTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTTAGATGCAGGGAtt > 1:1478436/1‑239 (MQ=255) ggcggcTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGGATTTCGCCAATGGCGGTGGGAGAGGGGATTgagagg > 1:677847/1‑229 (MQ=255) cggcTCCGGTATCCCGTTCATCAAACACCCCAAGCGCGAGTCGATGACCCGGGGTATTCCAGTGGTGATGTTGACCGCCCGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATAACCAAGCCGGTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGGAATGCGCCGCATTTCGCCAATGGGGGGGGGAAAGGGGAGAGAGATGCAGGGGTTaa > 2:2366646/1‑239 (MQ=255) cggcTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGTGAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGATTTAGATGCCGGGGttta > 2:2843226/1‑237 (MQ=255) cggcTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGATTGAGAAGCAGGGATTaa > 1:1139177/1‑239 (MQ=255) cggcTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGCGGAAGAGGGGATTGGAAAGCAGGGATTaa > 1:1694943/1‑239 (MQ=255) gcTCCGGTATCCAGTTCATCAAACCCCTCAAGCGCGAGTCGATGACCCGGGATATTCCCGTGGTGATGTTGACCGCCAGAGGGGACGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGCATCAAAGCGGTAATGCGCCGTATTTCGCCACTGGCGGTGGGAGACGTGGGTGCGATGGAgggtgtaat > 2:2850110/1‑232 (MQ=255) tCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGGTTGAGATGCAGGGGTTAAGtc > 1:3538211/1‑238 (MQ=255) tCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGAGGAAGAGGGGATTGAGATGCAGGGATTAAGtt > 1:3306675/1‑237 (MQ=255) gTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGGGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGGAATGCGCCGTATTTCGCCAATGGCGGGGGGAGAGGGGATTTAGATGCCGGGGTTAAGTCTCGa > 1:3399691/1‑238 (MQ=255) cAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGTGATGGAGATGCAGGGATGTAGGCTCGACCCGa > 2:308078/1‑238 (MQ=255) ttCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTTGGAGAGGTGGTTGAGATGCCGGGGTTTAGTCTCGGACCGAcct > 2:2992231/1‑236 (MQ=255) ttCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGCGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGGGTGAGGTGCAGGGGTTAAGTCTCGACCCGACa > 1:2975372/1‑237 (MQ=255) atcaAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACAtct‑c > 1:1055282/1‑238 (MQ=255) caAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAAGGATTAAGTCTCGACCCGACATCT‑CAcc > 1:2436018/1‑239 (MQ=255) caAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGCTGCAGGGATTAAGTCTCGACCCGCCATac‑cacc > 1:3468022/1‑239 (MQ=255) caAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CAcc > 1:307481/1‑239 (MQ=255) caAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGCAGAGGGGATTGAGATGCAGGGAGTAAGTCTCGAACCGACCTCT‑CAcc > 2:1012275/1‑239 (MQ=255) acCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGAGTGAGAGGCAGGGATTCAGTCTCCACCCGCCATCT‑CCCCGAgg > 2:2339420/1‑237 (MQ=255) ctaaaGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAACCCGACGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGAGCGAATCAAACCGGTAATGAGCCGTATTTCGCCAATGGCGGCGGCAGAGGGGCATGAGCTGCCAGGATTCAGTGCCTGCTCGCCATTT‑CACCGCGTGAt > 2:2401383/4‑239 (MQ=255) cTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTCTTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGAt > 1:1201126/1‑239 (MQ=255) cTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGCGGAAGAGGTGGATGAGATGCAGGGATTAAGTCCCGACCCGGCATCT‑CACCGaaggga > 2:2502956/1‑234 (MQ=255) tCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATg > 1:1519332/1‑239 (MQ=255) cAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGATTGAGATGCAGGGATTAAGCGTCCACACGACATCG‑CACCAGGTGATg > 2:3237303/1‑238 (MQ=255) cgAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGAAGAGGGGATTTGGATGCAGGGATTAAATGCCGACCCGACACCT‑CACCCAGTGATggg‑gggca > 2:3618100/1‑238 (MQ=255) agccGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGGAGGGATTaa > 1:1959038/4‑201 (MQ=255) aGTCGATGACCCGGGATATTCCAGTGGGGATGTTGACCGCCCGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGAGGTAATGCGCCGTATTTCGCCAATGGCGGCGGAAGAGGTGGTTGAGAAGGGTGGGTTAAGGCAGTGTCGCACGTCT‑CACCGCGTGATggc‑ggagtgg > 2:2928964/1‑234 (MQ=255) gggATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGGAGAGGTGATTGAGATGGAGGGGTTAAGTCTCGAGCCGACATCT‑CACCGAGGGGTGGC‑GGGTGGAGAGGCGCCgggg > 2:2868755/1‑237 (MQ=255) tatGCCAGTGGAGATGTGGACCGCCAGAGGAGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCTCCAAGCCGTTTTCGCCGAAGGGGCTGGTGGAGCGAATCAAAGCGGTAATTCGCCGTATTTCGCCAATGGCGGTTGAAGGGGTGATTGAGATTCAGGGATTAAGTCGCGAcc < 2:2171544/194‑1 (MQ=255) tCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGGTGGAGGGATTAAGGCTCGACCCGACATTC‑CACCGAGGGGTGGC‑GGGGGAAGAGCCCCTGGGGATgggg > 1:1674249/1‑238 (MQ=255) tCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGGAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CCCCGCGTGATGGC‑GGGGGGAGAGCGCCCGGGGATgggg > 1:3481016/1‑238 (MQ=255) gtggtgATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGCATTTCGCCAATGGCGGGGGAAGAGGTGATTGAGATGCAGGGATTAAGGCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGGGAAGAGCCGCGGGGGAGGGGGCCgcc > 1:1142416/1‑237 (MQ=255) gtgATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGGGAAGAGGCGCCGGGGGAGGGGcccgaaga > 1:1311317/1‑233 (MQ=255) gtgATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑Ca > 1:2171544/1‑194 (MQ=255) aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGGGGTGATTGGGATGCAggggt > 2:3482886/1‑167 (MQ=255) aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGGGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGGGTGATGGC‑GGGGGGAGAGCCCCCTGGGGTGGGGCCGGCAGG‑Att > 1:3586246/1‑239 (MQ=255) aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGATATCT‑CACCGAGTGATGGC‑GGGGGAAGAGCCGCCGGGGATGGGGcggc > 1:3523158/1‑229 (MQ=255) aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACCTCC‑CACCGCGGGATGGC‑GGGGGAAGAGCCGCCGGGGATGGGGCCGCCAGA‑Att > 1:2316687/1‑239 (MQ=255) aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGGGGAGAGACCCTGGGGATGGGGCCCGCAGa‑aat > 1:3160167/1‑237 (MQ=255) aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACCTCT‑CCACGAGTGATGGC‑GGGGGGAGAGCAGCCGGGGATGGGGCCGCCacagat > 2:3461614/1‑234 (MQ=255) gACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGAGGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGGGATGGC‑GGGGGAAAGGCCGCGGGGGAAGGGGCCGGCCGA‑ATTTaaaa > 1:2509676/1‑238 (MQ=255) gACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGGGAAGAGCCCCCGGGGGTGGGGCCGACAGA‑AGTTaaaa > 1:51410/1‑238 (MQ=255) ccAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGGGGGGATTGAGATGGAGGGGTTAAGTCCCGACCCCACATAC‑CCCCCGGGGATGGCGGGGCGAAGAG‑CGCGGGGGAGAGGGCCGACCCA‑Attataaaa > 2:397524/1‑229 (MQ=255) gaagaagaTCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGCGAAGAGCCGCTGGGGATGGGGGCGGCCGG‑ATTTAAAATGCTGCCAC‑ttttttt > 1:1824587/1‑238 (MQ=255) gaagaagaTCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGCGAAAAGCCGCTGGGGATGGGGCCGGCCGA‑ATTTTAACTGCTG‑CCC‑tttttttt > 1:1926082/1‑237 (MQ=255) gaTCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACCTCT‑CACCGAGTGATGGC‑GGGGGGAGAGGCGCCGGAGATGGGGGCGGCAGG‑AGTTTAAATGGTG‑CGC‑TTTTTTTTGaag > 2:813521/1‑235 (MQ=255) gaTCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGGTGGC‑GGGGGGAAAGGCGCCGGGGGTGGGGGCGGCCGAGATTTAAACGCGGG‑CAC‑ttttttttacgc > 2:2817879/1‑232 (MQ=255) cgTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGa > 1:1026250/1‑163 (MQ=255) tatCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAggg > 1:1875192/1‑102 (MQ=255) cACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGCGAAGAGCCGCTGGGGATGGGGCCGGCAGA‑ATTTAAACTGCTG‑CAC‑TTTTTTTTGACGCATCCCGa > 1:2701528/1‑204 (MQ=255) cACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGCGGAGAGCCGCTGGGGATGGGGCCGACAGA‑ATTTTAACTGCTG‑CCC‑TTTTTTATGACGCATCCTTAGCGCGTGTAC‑AACCGCGAGCAG‑CTGTTTAAacac > 2:819511/1‑238 (MQ=255) aCCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTGCGCCAATGGCGGTGGAAGAGGGGATGGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGA‑ATTTAAACTGCTG‑CAC‑TTTTTTATGACGCATCCTGAGCGCGTGTAC‑AGCCGCGAGCAG‑CTGTTAAACCACGt < 1:372843/239‑1 (MQ=255) ccAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCCATGGCGGAGGCAGGGGGGACTGAGATGACGGGATTAACTCTCGAACCGACCTCTCCACCG‑GGGATGGC‑GGGGGGAGAGACCCAAGGGCAGGGGCCGGCCGA‑TATTAAACTGCTT‑GACTTTTTTTTTTACGC‑TCCCGAGGGCGTGGTG‑CGCCGCGCGCAG‑CAGTTaatacact > 2:387024/1‑231 (MQ=255) aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACAt > 1:1623387/1‑114 (MQ=255) aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGGGGGAAGCCGCTGGGGGTGGGGGCGGCAGA‑ATTTTAACTTCTG‑CAC‑TTTTTTTTTACGCATACCTAGCGCGGGTTC‑AGCCCCGAGCAGCCGGTTAAACCCCgctgg > 2:1270542/1‑235 (MQ=255) aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGCGAAGAGCCGCTGGGGATGGGGGCGACAGA‑ATTTaaa > 1:191418/1‑170 (MQ=255) aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGGGGAGAGCCGCCGGGGAGGGGGGCGCAACA‑ATTTAAAAtgctg‑cg > 2:1801962/1‑177 (MQ=255) aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGGGGAGAGCCCCTGGGGATGGGGGCGACAGA‑ATTTtaa > 2:3556686/1‑170 (MQ=255) aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGCGAAGAGCCGCTGGGGATGGGGCCGACAGA‑ATTTAAACTGCTG‑CAC‑TTTTTTATGACGCATCCTGAGCGCGTGTAC‑AGCCGCGAGCAG‑CTGTTAAACCACGTCTgg > 1:3473389/1‑239 (MQ=255) aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGGAGAGGGGATTGAGATGCCGGGAGTAAGTCTCGACCCGACATCG‑CACCGAGGGATggc > 1:1196985/1‑130 (MQ=255) ccGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGGGGAAAGACGCCGGGGGTGGGGGCCGCAGA‑AATTTAACAGCTG‑CAC‑CTTTTTTTTACCCATCCTGCGCGCGTGTTCAACCCGCGAG‑AG‑CTGGTTAACCAcagcctgg > 2:939057/1‑231 (MQ=255) ttttCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGGTTAAGTCTCGACCCCACATCT‑CACCGAGTGATGGC‑GGGCGGAGAGACCCCGGGGGTGGGGGCGACCAA‑ATTTTAAATGCTG‑CAC‑CTTTTTTTGTCGCCTCCTCTGCGCGCGGTC‑AGCCCCGAGGCG‑CCGTTTTACCActccctcctta > 2:3499323/1‑228 (MQ=255) tgccccGAAGGAGGTGGTGGAGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTTATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGA‑ATTTAAACTGCTG‑CAC‑TTTTTTATGACGCATCCTGAGCGCGTGTAC‑AGCCGCGGGCAG‑CTGTTAAACCACGTCTGGGGAACt < 2:1759512/234‑1 (MQ=255) cggcGAAGTTGCTGGGGGCGATAATCAAAGCGGTAATGCGCCGGATTCCGCCAACGGCGGTGGAAGGCGTGATTTAGATTCAGGGATTAATTCTCGACCCGACATCT‑CACCGAGTTATGGC‑GGGCGAAGAGCCGCTGGAGATGGGGCCGAAAGA‑AATTAAACTGCTG‑CAC‑TTTTTTATGACGCGTCGTGATCGCGTGTAC‑AGCCGCGAGCAG‑CTGTTAAACCACGTCTGGGgaacgaac < 2:2061175/236‑1 (MQ=255) cGCCGAAGGAGCTGGTGGCACGAATCAAAGCGGTAATGCGCGGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCTGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGA‑ATTTAAACTGCTG‑CAC‑TTTTTTATGACGCATCCTGAGCGCGTGTAC‑AGCCGCGAGCAG‑CTGTTAAACCACGTCTGGGGAACTAAc < 2:1084301/239‑1 (MQ=255) cgAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGt > 1:3497224/1‑50 (MQ=255) cgAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGt < 2:3497224/50‑1 (MQ=255) tAATGCGCCGTATTTCGCCAATGGCGGTGGAAGGGGTGATTTAGATGTAGGGATTTAGTCCCGACCCGACATCT‑CACCGAGTGATGGC‑GGGGGAGGGAACGCTGGAGAAGGGGGCGGCAGA‑AAATaaa > 2:633394/1‑128 (MQ=255) tAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCAACCCGACATCT‑CACCGAGTGATGGC‑GGGCGAAGAGCCCCTGGAGATGGGGCCGACCAA‑ATTTAAACTGCTG‑GAC‑tttt > 1:1484886/1‑141 (MQ=255) gcCTTATTTCGCCAATGGCGGTGTAAGAGGTGAGTGAGATGCCGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGA‑ATTTAAACTGCTG‑CAC‑TTTTTTATGACGCATCCTGAGCGCGTGTAC‑AGCCGCGAGCAG‑CTGTTAAACCACGTCTGGGGAACTAACGTGTAT‑GTTGAAGACCGCACGGTCGATGTCCACATtcgt < 2:2725544/239‑1 (MQ=255) cGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGCGAAAAGCCGCTGGGGATGGGGCCGACAGA‑ATTTaaa > 1:3297632/1‑120 (MQ=255) tATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGGGATGGC‑GGGCGGAGAGCCGCTGGGGATGGGGGCGGCAGA‑AATTTAAATGCTG‑CCC‑CTTTTTTTGACGCATCCt > 2:1080872/1‑145 (MQ=255) gCCAATGGCGGTGGAAGAGGTGa < 2:812540/23‑1 (MQ=255) cAATTGAGGAGGAAGTGGTGACTGCGTTGCAGGGAGTAGGTCTCGACCCAGCAGCA‑CACCCTGGGATGCC‑GGGCGAAGCGCCGCTGGAGATGGGGCCGACATA‑CTTTAAACTTCTT‑CCC‑TTTTTTAGGACGCATCCTGAGCGCGTTTAC‑AGCCGCGATCAG‑CGGTTAAACCACGTCTTGCGATCTAACGTGTAT‑GTGGAAGACCGCACGTTCGATGTACTCATTCGTCGCCTGCGtga < 2:2957821/238‑3 (MQ=255) aaTGGCGGTGGAAGAGGTGATTg < 2:1875192/23‑1 (MQ=255) cGGTGGAAGAGGTGATTGAGATg < 2:1959038/23‑1 (MQ=255) ggTGGAAGAGGTGATTGAGATGc < 2:3044368/23‑1 (MQ=255) gTGGAAGAGGTGATTGAGATGCa < 2:679020/23‑1 (MQ=255) gTGGAAGAGGTGATTGAGATGCa < 2:692218/23‑1 (MQ=255) gTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGGGGAGAGCCGCTGGGGGTGGGGCCGACAGA‑AATTtaa > 2:80258/1‑102 (MQ=255) gTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGA‑ATTTAAACTGCTG‑CAC‑TTTTTTATGACGCATCCTGAGCGCGTGTAC‑AGCCGCGAGCAG‑CTGTTAAACCACGTCTGGGGAACTAACGTGTAT‑GTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGa > 1:1830607/1‑239 (MQ=255) gTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGCGAAGAGCCGCTGGGGATTGGGCCGACAGA‑ATTTAAACTGCTG‑CAC‑TTTTTTATGACGCATCCTCAGCCCGGGTAC‑AGCCGCGAGCGG‑CTGTTTAACCATGACTGGGGAACTAACGTGTATGGTGGAA‑ACCGCACGGGCGATGTCCACATTCGTGCTCTGCCGCAAGCACTGGCGccagcgcg > 2:691870/1‑233 (MQ=255) | CTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCT‑CACCGAGTGATGGC‑GGGCGAAGAGCCGCTGGAGATGGGGCCGACAGA‑ATTTAAACTGCTG‑CAC‑TTTTTTATGACGCATCCTGAGCGCGTGTAC‑AGCCGCGAGCAG‑CTGTTAAACCACGTCTGGGGAACTAACGTGTAT‑GTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGG > NC_000913/417295‑417770 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |