| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,151,686 | 0 | G | T | 48.5% | ‑6.2 / 20.7 | 34 | intergenic (+40/+25) | yegJ/yegK | uncharacterized protein/ser/thr phosphatase‑related protein |
| Reads supporting (aligned to +/- strand): ref base G (13/4); new base T (11/5); total (25/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.60e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTG‑TCT‑TCAACCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGC > NC_000913/2151448‑2151906 | tATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑ttg < 1:2231185/239‑1 (MQ=255)tATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑ttg < 2:3618810/239‑1 (MQ=255)tATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑ttg < 2:30758/239‑1 (MQ=255) tggtCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTG‑TCT‑TCAACCGCCATGAGAAAATCACTCTGCCCACAG‑CGCCAGTGCCAAAGTTTTATCATCATCCGTACGCTCGTTT‑ACCCCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑Ga > 1:1213227/4‑235 (MQ=255) gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTGA‑TTG‑TTT‑TCT‑TTAACCCCCATGAGAAAATCACTCTTCCCACAG‑CGCCAGTGCCCACGGTTTTTTCTCATCCCTTCCCTCGGTT‑ACCCCCCGGCTGGGCAAAAAATTGCTTTAACAA‑TCA‑AGTAATTAATC‑GA‑g > 2:2504495/1‑235 (MQ=255) gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTG‑GCT‑TCAAACCCCCTGAGAAAATCACTCTCCCCACCA‑CGCCCGTGGCCACGGTTTTTCATCATTCGTa > 1:812818/1‑176 (MQ=255) ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTT‑TTT‑TCAACCTCCATGAGAAATTTACTCTTTCCACAG‑CGCCAGTGGCAACGTTTTATCCTTAATCCCACGCTCGTTT‑ACCCCCCGGGCGGAC‑CAAAAAAGCTTTAACAATTTC‑GGTAATAAATC‑CA‑GTTGTTccgta > 2:488399/1‑236 (MQ=255) ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGGTTAA‑TTT‑TTG‑TTT‑TTAAACGCCATTAAAAAATCACTCTTCCCACAG‑CGCCAGGGGCAACGGTTTATCATCATTCGTACGCTCCTTT‑ACCGCCCGGCTGGACAAAAAATTGTTTTAACAA‑TCC‑GGGAATAAAACGGG‑GTTGTTctgc > 2:2610433/1‑236 (MQ=255) ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGGTGAA‑TTG‑TTG‑TCT‑TCAACCCCCATGAGAAACTCACTCTGCCCACAA‑CGCCAGTGCCAACGGTTTATCATCATCCGTACGCCCGTTT‑TCCCCCGGGCTGGGC‑AAAAAATGCTTTTACaaat > 1:864729/1‑211 (MQ=255) tATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTAA‑TTT‑TTT‑TCT‑TCAACCCCCATGAGAAAATCACTCTCCCCCCAG‑CCCCAGTGCCCCCGGTTTATCACCCTCCGTACGCCCGTTT‑ACCGCCCGGCCGGAC‑AAAAACTGCCTTAACAATTCA‑GGTAATAAATC‑GA‑gttgtt > 2:143536/1‑233 (MQ=255) tATTGGTCACCTATCAAAAAGAAGAAGCCGACGAGCAAAGAGAAAAATTAGTT‑CGCTAATCAGCCGTTATTTTTATTTTTTAAACGGGCGGTTAAA‑TTG‑TTG‑TCT‑TAAACCGCCATGAGAAACTCACTCTg < 1:3290773/131‑1 (MQ=255) gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTT‑TTG‑TCT‑TCAACCCCCATGAGAAACTAACTCTGcccc > 1:2897585/1‑129 (MQ=255) gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTG‑TCT‑TCAACCGCCATGAGAAACTCACTCTGCCca > 1:727283/1‑130 (MQ=255) cTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTT‑TTT‑TCT‑TCAACCCCCATCAGAAAATCACCCTTCCCAAAa‑cgc > 2:3290773/1‑131 (MQ=255) cTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTT‑TCT‑TCAACCGCCATGAGAAAATCACTCTGCCCCCAG‑CGCCAGTGCCCACGTTTTTTCATCAACCGTACGCCCGGTT‑ACCGCCGGGGTGGAC‑AAAAAATGCTTTTACAATTCA‑GGTAATAAAAC‑GA‑GGTGTTCCTTCGGTGCTgccc > 2:1105884/1‑237 (MQ=255) aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTT‑TCT‑TCAAACCCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTatc > 1:1521234/1‑147 (MQ=255) aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTG‑TCT‑TCAACCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTTCTgccgccc > 1:1190915/1‑238 (MQ=255) aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGATGGA‑ATG‑TTG‑TTT‑TTCAACGCCACCAGAAAATCCCTCTCGCCCCCG‑CGCCCGGGGCCAAGGTTTATCATCACCCCGACTCTCCTTTAACCGCCGGCCGGGGA‑AAAACATGCTTTAAC‑CTTCAGGGAAATAAATA‑GG‑GTTTTTACTGCGTtgcgcgcccc > 2:1430373/1‑232 (MQ=255) aaaaaaaAGAACCCGACGAGTAAAGAAAAAATTTAGTT‑GCTTAATCGGCCTTTATTTTTTTTTTTAAAACGGGCGTTTAAT‑TTT‑TTT‑TCT‑CCACCCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTc < 1:2413710/238‑1 (MQ=255) aaaaaaGAAGCGGACGAGCAAAGAAC‑AAATTGGTTGCGATAATCAGCTGTTAT‑TTTTTTTTTTAAACCGGGGGTTTAA‑TTT‑TTGTTCT‑CCAACCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTCCa < 1:2850793/239‑1 (MQ=255) aagaagaagCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTT‑TTT‑TCAACCGCCATTAGAAACTCCCTCTTCCCACAG‑CGCCCGTGCCCACGTTTTTTCATCATCCCTACGCTCGTTT‑TCCCCCGGGCTGGGC‑AAAAACTGCTTTTACAATTCA‑GGTAATAAAAC‑AC‑GTTGTTCCTGCTTTTCTgccg > 2:1189856/1‑230 (MQ=255) gaagCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGGA‑TTT‑TTT‑TCT‑TCAAACGCCCTTAGAAACTTACCCTTCCca > 2:1464902/1‑112 (MQ=255) gaagCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGGA‑TTA‑TTT‑TCT‑TTCACCACCCTGATAAACTTACTCTGCCCACAGCCCCCGGGGCCAAC‑TTTTATTATTATTCCTGAGCTCGTTT‑TACGCCCGGCTGGGC‑AAAAACAGCCTTAACAAATTA‑GTGCATAAATT‑GA‑GTGTTTTCTGCCTTGCGTGCTCCCGTCCATTGaaa > 2:1989390/1‑239 (MQ=255) ccGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑ATT‑TTT‑TCT‑TCCACCGCCATTAGAAAATCACTCTTCCCACAA‑CGCCCGTGCCCACGTTTTTTCATAATCCCTACGCTCGTTT‑ATCGCCCGGCCGGGC‑AAAAACAGCTTTTAAAATTCA‑GGTAATAAAAC‑AC‑GGTGTTTCTTCGTTGCTGCCGCCAATCCCTTTaaaaaaa > 2:2708274/1‑237 (MQ=255) aaTTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTAA‑ATT‑TTT‑TCT‑TTAACCGCCCTTAGAAACTCACTCTGCCCCCAG‑CGCCCGTGCCCAAGTTTTTTCATCATCCGTACGCTCGTTT‑TCCGCCCGGCTGGGC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAAAC‑GA‑GATGTTCCTCCGTTGCTGTCGCCCGTCCCTTTTAAAAAGGGGTGAAAAAACGcacaa > 2:3180874/1‑238 (MQ=255) tttgTT‑CTCTAACCAGCTTTTATTTTTATTTTTAAAGGTGGCGGTAGAT‑TTG‑TTG‑TCC‑CAAACCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATga < 1:22367/236‑1 (MQ=255) cTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTAATTTT‑TTT‑TTT‑TCAACC‑CCATGATAAAATAACTCTCCCCCCAG‑CGCCAGTGGCAACGTTTTATCATCCTCCGTACCCCCGTTT‑ACCGCCGGGCGGGGC‑AAAAACTCCTTTAACAATTCA‑GGGAATAAATT‑GA‑GTAGTTTCTGCGGTTCCTCCGCCCATACATTGAAAAACAGGGGAAAAAAAGTAACATGAGGAGAAtt > 2:1156394/1‑239 (MQ=255) aaTCGGCTGTTTTTTTTTTTTTTAAAACGGGGGTTTGAT‑TTT‑TTT‑TTT‑AAACCCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTAt < 1:1073747/239‑1 (MQ=255) cttttATATTTTTTGTTAAAACGGGGGTTTAAT‑TTT‑TTC‑TCT‑ACAACCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCa < 1:2600569/236‑1 (MQ=255) cGTTATATTTATTGTTTAAACTGGCGGTTGAA‑ttg < 2:1535556/35‑1 (MQ=255) ttATATTTATTGTTTAAACTGGCGGTTGAA‑TTT‑TTT‑TCT‑TCAACCCCCCTGAGAAACTCACTCTGCCCCCAG‑CGCCCGTGCCCACGTTTTATTATCATCCCTACGCTCGTTT‑ACCCCCCGGCTGGGC‑AAAAAATTCTTTTACAAATCC‑GGTAATAAAAC‑GAGGTTGTT‑CTGCGATGTTGCCGCCAGACCATTGAAAAAAGGGGGTAAAAAAGGGACAGCAGGGGAAATATcaaaaacat > 2:1572128/1‑231 (MQ=255) tattTTTTTTTTTAAAGCGGGGGGTTAAT‑TTT‑TTG‑TCT‑TCACCCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTatca < 2:1635667/93‑1 (MQ=255) atttattGTTTAAACTGGCGGTTGAA‑ATG‑TTG‑TCT‑TCAACCGCCATGAGAAAATCACTCTGCCCACAG‑CGCCCGTGCCCACGtttttt > 1:3242157/1‑86 (MQ=255) attGTTTAAACTGGCGGATGAA‑TTG‑TTG‑TCT‑TCAACCGCCTTGAGAAAATCACTCTGCCCACAA‑CGACAGAGGCAACGATTTATTATCATCAGTAAGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAAATTGTTTAAAAATTTA‑AGTAAAAAATA‑GA‑GTTGTTCCTGCGGTGCTGCAGCCAGTCCATTTAAAAACGGGGGAAAGAAAGGGACATGAGGAGAGATATCCCGCATATTTaaag > 2:13511/1‑235 (MQ=255) tGTTTAAACTGGCGGTTGGA‑TTT‑TTT‑TCT‑TCCAACCCCCTGAGAAAATTACTCTGCCCACAG‑CGCCAGTGCCCACGGTTTATCATCCTCCGTACGCTCGTTT‑ATCGCCGGGCTGGAC‑AAAAAATGCTTTAACAATTTC‑GGGAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGGGAATTTTCCAGCATATTTAACGcc > 2:2059680/1‑237 (MQ=255) gTTTAAACTGGCGGTTGAA‑ATT‑TTT‑TTT‑TCCACCCCCCTGAGAAAATCAATCTTGCCCCAG‑CGCCAGGGCCAACCGTTTATTATAATCCGGACGCTCGTTT‑AACGCCGGGGCGGAC‑AAAAAATCCTTTAACAAATCA‑GGGAATAAATC‑GA‑GTTGTTCCTGCGTTGCCGGCCCCAGTCCATTGAAAAAAAGGGGAAAGAAAAGGACATTAGGAAGAATAGTCACCATATTTTACgtcca > 2:904877/1‑234 (MQ=255) taacgcgggggTTGAA‑TTGTTTT‑TCT‑TCAACCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCg < 1:2792716/230‑1 (MQ=255) aaaCTGGGTGGTGAA‑TTG‑TTG‑TCTACCACCCGCCATGAGAAACTCCCTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGc < 1:2815274/239‑1 (MQ=255) | TATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTG‑TCT‑TCAACCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGC > NC_000913/2151448‑2151906 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |