Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,151,6890GT54.5% ‑3.4 / 19.1 34intergenic (+43/+22)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (10/5);  new base T (15/3);  total (25/9)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTG‑TCT‑TCAACCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAT  >  NC_000913/2151580‑2151924
                                                                                                                |                                                                                                                                                                                                                                                   
tggtCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTG‑TCT‑TCAACCGCCATGAGAAAATCACTCTGCCCACAG‑CGCCAGTGCCAAAGTTTTATCATCATCCGTACGCTCGTTT‑ACCCCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑Ga                                                                                                                 >  1:1213227/4‑235 (MQ=255)
 gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTGA‑TTG‑TTT‑TCT‑TTAACCCCCATGAGAAAATCACTCTTCCCACAG‑CGCCAGTGCCCACGGTTTTTTCTCATCCCTTCCCTCGGTT‑ACCCCCCGGCTGGGCAAAAAATTGCTTTAACAA‑TCA‑AGTAATTAATC‑GA‑g                                                                                                               >  2:2504495/1‑235 (MQ=255)
 gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTG‑GCT‑TCAAACCCCCTGAGAAAATCACTCTCCCCACCA‑CGCCCGTGGCCACGGTTTTTCATCATTCGTa                                                                                                                                                                               >  1:812818/1‑176 (MQ=255)
       ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTT‑TTT‑TCAACCTCCATGAGAAATTTACTCTTTCCACAG‑CGCCAGTGGCAACGTTTTATCCTTAATCCCACGCTCGTTT‑ACCCCCCGGGCGGAC‑CAAAAAAGCTTTAACAATTTC‑GGTAATAAATC‑CA‑GTTGTTccgta                                                                                                     >  2:488399/1‑236 (MQ=255)
       ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGGTTAA‑TTT‑TTG‑TTT‑TTAAACGCCATTAAAAAATCACTCTTCCCACAG‑CGCCAGGGGCAACGGTTTATCATCATTCGTACGCTCCTTT‑ACCGCCCGGCTGGACAAAAAATTGTTTTAACAA‑TCC‑GGGAATAAAACGGG‑GTTGTTctgc                                                                                                      >  2:2610433/1‑236 (MQ=255)
       ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGGTGAA‑TTG‑TTG‑TCT‑TCAACCCCCATGAGAAACTCACTCTGCCCACAA‑CGCCAGTGCCAACGGTTTATCATCATCCGTACGCCCGTTT‑TCCCCCGGGCTGGGC‑AAAAAATGCTTTTACaaat                                                                                                                                  >  1:864729/1‑211 (MQ=255)
        tATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTAA‑TTT‑TTT‑TCT‑TCAACCCCCATGAGAAAATCACTCTCCCCCCAG‑CCCCAGTGCCCCCGGTTTATCACCCTCCGTACGCCCGTTT‑ACCGCCCGGCCGGAC‑AAAAACTGCCTTAACAATTCA‑GGTAATAAATC‑GA‑gttgtt                                                                                                          >  2:143536/1‑233 (MQ=255)
        tATTGGTCACCTATCAAAAAGAAGAAGCCGACGAGCAAAGAGAAAAATTAGTT‑CGCTAATCAGCCGTTATTTTTATTTTTTAAACGGGCGGTTAAA‑TTG‑TTG‑TCT‑TAAACCGCCATGAGAAACTCACTCTg                                                                                                                                                                                                                      <  1:3290773/131‑1 (MQ=255)
             gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTT‑TTG‑TCT‑TCAACCCCCATGAGAAACTAACTCTGcccc                                                                                                                                                                                                                  >  1:2897585/1‑129 (MQ=255)
             gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTG‑TCT‑TCAACCGCCATGAGAAACTCACTCTGCCca                                                                                                                                                                                                                  >  1:727283/1‑130 (MQ=255)
                  cTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTT‑TTT‑TCT‑TCAACCCCCATCAGAAAATCACCCTTCCCAAAa‑cgc                                                                                                                                                                                                           >  2:3290773/1‑131 (MQ=255)
                  cTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTT‑TCT‑TCAACCGCCATGAGAAAATCACTCTGCCCCCAG‑CGCCAGTGCCCACGTTTTTTCATCAACCGTACGCCCGGTT‑ACCGCCGGGGTGGAC‑AAAAAATGCTTTTACAATTCA‑GGTAATAAAAC‑GA‑GGTGTTCCTTCGGTGCTgccc                                                                                           >  2:1105884/1‑237 (MQ=255)
                    aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTT‑TCT‑TCAAACCCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTatc                                                                                                                                                                                         >  1:1521234/1‑147 (MQ=255)
                    aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTG‑TCT‑TCAACCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTTCTgccgccc                                                                                        >  1:1190915/1‑238 (MQ=255)
                    aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGATGGA‑ATG‑TTG‑TTT‑TTCAACGCCACCAGAAAATCCCTCTCGCCCCCG‑CGCCCGGGGCCAAGGTTTATCATCACCCCGACTCTCCTTTAACCGCCGGCCGGGGA‑AAAACATGCTTTAAC‑CTTCAGGGAAATAAATA‑GG‑GTTTTTACTGCGTtgcgcgcccc                                                                                         >  2:1430373/1‑232 (MQ=255)
                       aaaaaaaAGAACCCGACGAGTAAAGAAAAAATTTAGTT‑GCTTAATCGGCCTTTATTTTTTTTTTTAAAACGGGCGTTTAAT‑TTT‑TTT‑TCT‑CCACCCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTc                                                                                     <  1:2413710/238‑1 (MQ=255)
                         aaaaaaGAAGCGGACGAGCAAAGAAC‑AAATTGGTTGCGATAATCAGCTGTTAT‑TTTTTTTTTTAAACCGGGGGTTTAA‑TTT‑TTGTTCT‑CCAACCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTCCa                                                                                   <  1:2850793/239‑1 (MQ=255)
                          aagaagaagCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTT‑TTT‑TCAACCGCCATTAGAAACTCCCTCTTCCCACAG‑CGCCCGTGCCCACGTTTTTTCATCATCCCTACGCTCGTTT‑TCCCCCGGGCTGGGC‑AAAAACTGCTTTTACAATTCA‑GGTAATAAAAC‑AC‑GTTGTTCCTGCTTTTCTgccg                                                                                           >  2:1189856/1‑230 (MQ=255)
                               gaagCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGGA‑TTT‑TTT‑TCT‑TCAAACGCCCTTAGAAACTTACCCTTCCca                                                                                                                                                                                                                  >  2:1464902/1‑112 (MQ=255)
                               gaagCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGGA‑TTA‑TTT‑TCT‑TTCACCACCCTGATAAACTTACTCTGCCCACAGCCCCCGGGGCCAAC‑TTTTATTATTATTCCTGAGCTCGTTT‑TACGCCCGGCTGGGC‑AAAAACAGCCTTAACAAATTA‑GTGCATAAATT‑GA‑GTGTTTTCTGCCTTGCGTGCTCCCGTCCATTGaaa                                                                             >  2:1989390/1‑239 (MQ=255)
                                   ccGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑ATT‑TTT‑TCT‑TCCACCGCCATTAGAAAATCACTCTTCCCACAA‑CGCCCGTGCCCACGTTTTTTCATAATCCCTACGCTCGTTT‑ATCGCCCGGCCGGGC‑AAAAACAGCTTTTAAAATTCA‑GGTAATAAAAC‑AC‑GGTGTTTCTTCGTTGCTGCCGCCAATCCCTTTaaaaaaa                                                                         >  2:2708274/1‑237 (MQ=255)
                                                     aaTTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTAA‑ATT‑TTT‑TCT‑TTAACCGCCCTTAGAAACTCACTCTGCCCCCAG‑CGCCCGTGCCCAAGTTTTTTCATCATCCGTACGCTCGTTT‑TCCGCCCGGCTGGGC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAAAC‑GA‑GATGTTCCTCCGTTGCTGTCGCCCGTCCCTTTTAAAAAGGGGTGAAAAAACGcacaa                                                       >  2:3180874/1‑238 (MQ=255)
                                                       tttgTT‑CTCTAACCAGCTTTTATTTTTATTTTTAAAGGTGGCGGTAGAT‑TTG‑TTG‑TCC‑CAAACCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATga                                                     <  1:22367/236‑1 (MQ=255)
                                                                cTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTAATTTT‑TTT‑TTT‑TCAACC‑CCATGATAAAATAACTCTCCCCCCAG‑CGCCAGTGGCAACGTTTTATCATCCTCCGTACCCCCGTTT‑ACCGCCGGGCGGGGC‑AAAAACTCCTTTAACAATTCA‑GGGAATAAATT‑GA‑GTAGTTTCTGCGGTTCCTCCGCCCATACATTGAAAAACAGGGGAAAAAAAGTAACATGAGGAGAAtt                                             >  2:1156394/1‑239 (MQ=255)
                                                                  aaTCGGCTGTTTTTTTTTTTTTTAAAACGGGGGTTTGAT‑TTT‑TTT‑TTT‑AAACCCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTAt                                           <  1:1073747/239‑1 (MQ=255)
                                                                        cttttATATTTTTTGTTAAAACGGGGGTTTAAT‑TTT‑TTC‑TCT‑ACAACCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCa                                     <  1:2600569/236‑1 (MQ=255)
                                                                           ttATATTTATTGTTTAAACTGGCGGTTGAA‑TTT‑TTT‑TCT‑TCAACCCCCCTGAGAAACTCACTCTGCCCCCAG‑CGCCCGTGCCCACGTTTTATTATCATCCCTACGCTCGTTT‑ACCCCCCGGCTGGGC‑AAAAAATTCTTTTACAAATCC‑GGTAATAAAAC‑GAGGTTGTT‑CTGCGATGTTGCCGCCAGACCATTGAAAAAAGGGGGTAAAAAAGGGACAGCAGGGGAAATATcaaaaacat                                  >  2:1572128/1‑231 (MQ=255)
                                                                            tattTTTTTTTTTAAAGCGGGGGGTTAAT‑TTT‑TTG‑TCT‑TCACCCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTatca                                                                                                                                                                                        <  2:1635667/93‑1 (MQ=255)
                                                                               atttattGTTTAAACTGGCGGTTGAA‑ATG‑TTG‑TCT‑TCAACCGCCATGAGAAAATCACTCTGCCCACAG‑CGCCCGTGCCCACGtttttt                                                                                                                                                                                          >  1:3242157/1‑86 (MQ=255)
                                                                                   attGTTTAAACTGGCGGATGAA‑TTG‑TTG‑TCT‑TCAACCGCCTTGAGAAAATCACTCTGCCCACAA‑CGACAGAGGCAACGATTTATTATCATCAGTAAGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAAATTGTTTAAAAATTTA‑AGTAAAAAATA‑GA‑GTTGTTCCTGCGGTGCTGCAGCCAGTCCATTTAAAAACGGGGGAAAGAAAGGGACATGAGGAGAGATATCCCGCATATTTaaag                            >  2:13511/1‑235 (MQ=255)
                                                                                     tGTTTAAACTGGCGGTTGGA‑TTT‑TTT‑TCT‑TCCAACCCCCTGAGAAAATTACTCTGCCCACAG‑CGCCAGTGCCCACGGTTTATCATCCTCCGTACGCTCGTTT‑ATCGCCGGGCTGGAC‑AAAAAATGCTTTAACAATTTC‑GGGAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGGGAATTTTCCAGCATATTTAACGcc                          >  2:2059680/1‑237 (MQ=255)
                                                                                      gTTTAAACTGGCGGTTGAA‑ATT‑TTT‑TTT‑TCCACCCCCCTGAGAAAATCAATCTTGCCCCAG‑CGCCAGGGCCAACCGTTTATTATAATCCGGACGCTCGTTT‑AACGCCGGGGCGGAC‑AAAAAATCCTTTAACAAATCA‑GGGAATAAATC‑GA‑GTTGTTCCTGCGTTGCCGGCCCCAGTCCATTGAAAAAAAGGGGAAAGAAAAGGACATTAGGAAGAATAGTCACCATATTTTACgtcca                        >  2:904877/1‑234 (MQ=255)
                                                                                         taacgcgggggTTGAA‑TTGTTTT‑TCT‑TCAACCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCg                     <  1:2792716/230‑1 (MQ=255)
                                                                                          aaaCTGGGTGGTGAA‑TTG‑TTG‑TCTACCACCCGCCATGAGAAACTCCCTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGc                    <  1:2815274/239‑1 (MQ=255)
                                                                                                                g‑tCT‑TCAACCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGc                                                                                                                                            <  1:3360523/101‑1 (MQ=255)
                                                                                                                g‑tCT‑TCAACCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGc                                                                                                                                            >  2:3360523/1‑101 (MQ=255)
                                                                                                                g‑tCT‑TCAACCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGt                                                                                      >  1:40147/1‑152 (MQ=255)
                                                                                                                g‑tCT‑TCAACCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAt  >  2:426850/1‑236 (MQ=255)
                                                                                                                |                                                                                                                                                                                                                                                   
TGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTG‑TTG‑TCT‑TCAACCGCCATGAGAAACTCACTCTGCCCACAG‑CGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTT‑ACCGCCGGGCTGGAC‑AAAAACTGCTTTAACAATTCA‑GGTAATAAATC‑GA‑GTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAT  >  NC_000913/2151580‑2151924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑