New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4516891 =89 (0.930)7 (0.080) 6/544 8.7 7.5% coding (827/954 nt) fecR anti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator
?NC_000913 4516925 = 87 (0.940)coding (793/954 nt) fecR anti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGCTTTAGGGACAGAATTTACCGTCCGCCAGCAGGATAATTTCACGCAGCTTGACGTGCAGCAGCACGCTGTGGAAGTGCTTCTCGCCAGTGCCCCCGCGCAAAAACGCATCGTGAACGCTGGTGAAAGCCTGCAGTTCAGCGCCTCTGAGTTTGGCGCAGTGAAACCGCTGGATGACGAGAGTACAAGCTGGACGAAGGACATCCTGAGCTTCAGCGATAAACCGCTGGGTGAGGTGATAGCCACGCTAACCCGTTACCGCAACGGCGTGCTGCGCTGCGATCCCGCCGTTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4517184‑4516891
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgccgttgcGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACAC  >  NC_000913/4516925‑4517212
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
TGCTCTAGGGACAGAATTTACCGTCCGCCAGCAGGATAATTTCACGCAGCTTGACGTGCAGCAGCACGCTGTGGAAGTGCTTCTCGCCAGTGCCCCCGCGCAAAAACGCATCGTGAACGCTGGTGAAAGCCTGCAGTTCAGCGCCTCTGAGCTTGGCGCAGTGAAACCGCCGGATGACGAGAGTACAAGCTGGACGAAGGACATCCTGAGCTTCAGCGATAAACCGCTGGGTGAGGTGATAGCCACGCTAACCCGTTACCGCAACGGCGTGCTGCGCTGCGATCCCGgc                                                                                                                                                                                                                                                                                                       >  1:552530/1‑287
     TAGGGACAGAATTTACCGTCCGCCAGCAGGATAATTTCACGCAGCTTGACGTGCAGCAGCACGCTGTGGAAGTGCTTCTCGCCAGTGCCCCCGCGCAAAAACGCATCGTGAACGCTGGTGAAAGCCTGCAGTTCAGCGCCTCTGAGTTTGGCGCAGTGAAACCGCTGGATGACGAGGGTACAAGCTGGACGAAGGACATCCTGAGCTTCAGCGATAAACCGCTGGGTGAGGTGATAGCCACGCTAACCCGTTACCGCAACGGCGTGCTGCGCTGCGATCCCGCCGTTGC                                                                                                                                                                                                                                                                                                  >  2:144924/1‑289
            AGAATTTACCGTCCGCCAGCAGGATAATTTCACGCAGCTTGACGTGCAGCAGCACGCTGTGGAAGTGCTTCTCGCCAGTGCCCCCGCGCAAAAACGCATCGTGAACGCTGGTGAAAGCCTGCAGTTCAGCGCCTCTGAGTTTGGCGCAGTGAAACCGCTGGATGACGAGAGTACAAGCTGGACGAAGGACATCCTGAGCTTCAGCGATAAACCGCTGGGTGAGGTGATAGCCACGCTAACCCGTTACCGCAACGGCGTGCTGCGCTGCGATCCCGCCGTTGCGGTAACG                                                                                                                                                                                                                                                                                           >  1:299896/1‑289
            AGAATTTACCGTCCGCCAGCAGGATAATTTCACGCAGCTTGACGTGCAGCAGCACGCTGTGGAAGTGCTTCTCGCCAGTGCCCCCGCGCAAAAACGCATCGTGAACGCTGGTGAAAGCCTGCAGTTCAGCGCCTCTGAGTTTGGCGCAGTGAAACCGCTGGATGACGAGAGTACAAGCTGGACGAAGGACATCCTGAGCTTCAGCGATAAACCGCTGGGTGAGGTGATAGCCACGCTAACCCGTTACCGCAACGGCGTGCTGCGCTGCGATCCCGCCGTTGCGGTAACG                                                                                                                                                                                                                                                                                           >  1:658081/1‑289
                         CGCCGGCAGGGTAATGTCGCGCGGCGTGACGGGCAGCAGCACGCTGTGGAAGTGCTTCTCGCCAGTGCCCCCGCGCAAAAGCGCATCGTGAACGCTGGTGAAGGCCTGCAGTTCAGCGCCTCTGAGTTTGGCGCAGTGAAACCGCTGGATGACGAGAGTACAAGCTGGACGAAGGACATCCTGGGCTTCAGCGATAAACCGCTGGGTGAGGTGATAGCCACGCTAACCCGTTACCGCAACGGCGTGCTGCGCTGCGATCCCGCCGTTGCGGTAACGGGTTAGCGTGGCT                                                                                                                                                                                                                                                                              <  2:658081/289‑1
                                    TAATTTCACGCAGCTTGACGTGCAGAGGCACGCTGTGCAAGTGCTTCTCGCCAGTGCCCCCGCGCAAAAACGCATCGTGAACGCTGGTGAAAGCCTGCAGTTCAGCGCCTCTGAGTTTGGCGCAGTGAAACCGCTGGATGACGAGAGTACAAGCTGGACGAAGGACATCCTGAGCTTCAGCGATAAACCGCTGGGTGAGGTGATAGCCACGCTAACCCGTTACCGCAACGGCGTGCTGCGCTGCGATCCCGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACC                                                                                                                                                                                                                                                                   <  2:299896/289‑1
                                               AGCTTCACGTGCAGCAGCATGCTGTGGAAGGGCTTCTCGCCAGTGCCCCCGCGCAAAAACGCATCGTGAACGCTGGTGAAAGCCTGCAGTTCAGCGCCTCTGAGTTTGGCGCAGTGAAACCGCTGGATGACGAGAGTACAAGCTGGACGAAGGACATCCTGAGCTTCAGCGATAAACCGCTGGGTGAGGTGATAGCCACGCTAACCCGTTACCGCAACGGCGTGCTGCGCTGCGATCCCGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTAT                                                                                                                                                                                                                                                        <  2:407746/289‑1
                                                 CTTGACGTGCAGCAGCACGCTGTGGAAGTGCTTCTCGCCAGTGCCCCCGCGCAAAAACGCATCGTGAACGCTGGTGAAAGCCTGCAGTTCAGCGCCTCTGAGTTTGGCGCAGTGAAACCGCTGGATGACGAGAGTACAAGCTGGACGAAGGACATCCTGAGCTTCAGCGATAAACCGCTGGGTGAGGTGATAGCCACGCTAACCCGTTACCGCAACGGCGTGCTGCGCTGCGATCCCGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCG                                                                                                                                                                                                                                                      >  1:407746/1‑289
                                                                         gaaagGCTTCTAGCCAGTGCCAAAGCGCAAAAACGAATCGTGAACGCTGGTGAAAGCCAGCAGTTCAGAGCCTCTGAGTTTGGCGAAGTGAAACCGCTGGATGAAGAGAGTACAAGCTGAACGAAGGACATCCTGAGCTTCAGCGAGAAACCGCTGGGTGAGGTGATAGCAACGATAACCCGTTAACGCAACGGCGTGCTGCGCTGCGATCCCGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGT                                                                                                                                                                                                                               <  1:26109/283‑1
                                                                                                                                                                                                                                                                                             CGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGT          <  1:312581/289‑1
                                                                                                                                                                                                                                                                                             CGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGT          <  1:62998/289‑1
                                                                                                                                                                                                                                                                                              GCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGCGCTGCTGCACGTCAAGCTGCGTGAAATTAACCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACCGGT          >  1:456351/1‑288
                                                                                                                                                                                                                                                                                                  TTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGA     <  1:789765/289‑1
                                                                                                                                                                                                                                                                                                    GCGGTAACGGTTTAGCGTAGCTATCTCCTCACCCAGCGGTTTATCGCTGGAGCTCAGGATTTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGTTGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGAC    <  1:736730/288‑1
                                                                                                                                                                                                                                                                                                     CGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACA   <  1:512130/288‑1
                                                                                                                                                                                                                                                                                                     CGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTACGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCGGTCCCTAAACCAGTGAGATGGACCTGCCGGGTCAGGACAC  >  1:304514/1‑289
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
TGCTTTAGGGACAGAATTTACCGTCCGCCAGCAGGATAATTTCACGCAGCTTGACGTGCAGCAGCACGCTGTGGAAGTGCTTCTCGCCAGTGCCCCCGCGCAAAAACGCATCGTGAACGCTGGTGAAAGCCTGCAGTTCAGCGCCTCTGAGTTTGGCGCAGTGAAACCGCTGGATGACGAGAGTACAAGCTGGACGAAGGACATCCTGAGCTTCAGCGATAAACCGCTGGGTGAGGTGATAGCCACGCTAACCCGTTACCGCAACGGCGTGCTGCGCTGCGATCCCGCCGTTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4517184‑4516891
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgccgttgcGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACAC  >  NC_000913/4516925‑4517212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.