Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,505,0880CT42.1% 41.1 / 15.0 38S311S (TCG→TCA) yhfSFNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base C (2/20);  new base T (16/0);  total (18/20)
Fisher's exact test for biased strand distribution p-value = 6.88e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGCA‑CCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGC‑GCTTTTCACTTCCGGCACCGCACCA‑CCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAG  >  NC_000913/3504857‑3505306
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                              
ccccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGGTTTATATTTTTA‑ctcc                                                                                                                                                                                                                         >  2:895021/1‑237 (MQ=255)
           aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTAGCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGCA‑CCCACCggg                                                                                                                                                                                                            <  2:428454/239‑1 (MQ=255)
                tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGATTTATATTTTTG‑CTCTCCACCCCCCCGGGTaag                                                                                                                                                                                                        >  1:93705/1‑239 (MQ=255)
                  tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTTATATTTTGC‑CACTGCA‑CCCACCCGGGaaagtg                                                                                                                                                                                                     >  1:1313174/1‑235 (MQ=255)
                              cGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGGTTCATATTTTGA‑CACTTCA‑CCCACCGGGGAAAGCCAGGCAccagc                                                                                                                                                                                           >  2:158769/1‑234 (MQ=255)
                                     aCCGTTCAGTACGTAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCGCGAAAAATCCGCAGCCCCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGCG‑CCCACCGGGTAAGGCAAGGCACCGCGCTTTTgcg                                                                                                                                                                                   <  2:976401/238‑1 (MQ=255)
                                                   aaTTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGGTTTTTATTTTGT‑CTCTGCC‑CACCCCCGGGAAGGGAAGGGCCCCCGCCTTCTTGCCTCTTCCACCAct                                                                                                                                                                     >  2:746342/1‑238 (MQ=255)
                                                      ttAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGCA‑CCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTgg                                                                                                                                                                 <  1:1555266/239‑1 (MQ=255)
                                                        aGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGTTTCTTATTTTGA‑CTCTTCA‑CCCACCGGGGGAGGGAAGGCGCCGCGCTTTTTCTCCTCTTccccccc                                                                                                                                                                      >  2:381362/1‑227 (MQ=255)
                                                                     tATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGca‑ccca                                                                                                                                                                                                                 <  1:184456/176‑1 (MQ=255)
                                                                       tCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGCA‑CCCACCGGGTAAGGCAAGGCACcg                                                                                                                                                                                             <  1:1485524/194‑1 (MQ=255)
                                                                            cATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTg                                                                                                                                                                                                                        <  1:1011484/163‑1 (MQ=255)
                                                                             aTTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGCA‑CCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGcc                                                                                                                                                                                 <  1:1170982/200‑1 (MQ=255)
                                                                               tAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGTTTTATATTTTGC‑ATCTGCA‑CCCCCCGGGGaagg                                                                                                                                                                                                       >  2:184456/1‑176 (MQ=255)
                                                                                 aaTACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGATTTATATTTTGA‑CCCCTCA‑CCCCCCCGGTGAGGCAAAGCACCCCGCctttttg                                                                                                                                                                                   >  2:1485524/1‑191 (MQ=255)
                                                                                      tGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGTTTTCTATTTTTA‑CCTTGca‑cccccccg                                                                                                                                                                                                             >  2:1011484/1‑161 (MQ=255)
                                                                                       ggCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGTTTCATATTTCGA‑CTCTTCA‑CCCACCCGGGAAGGGACGGGACCCCGCTTTTTCTCCTCTTccccaa                                                                                                                                                                       >  2:1170982/1‑196 (MQ=255)
                                                                                        gCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGTTTCCTCTTTTGA‑CTCTGCA‑CCCACCCGGGAAGGCAAGGCAACGCGCTTTTGCGCCCCTTCCACCACTCTGGGGGCCATCGGCCGATGAAACTCGaacaacaaac                                                                                                                                >  2:379796/1‑229 (MQ=255)
                                                                                        gCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATGCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGCC‑CCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGa                                                                                                                                         <  1:726591/229‑1 (MQ=255)
                                                                                              ctctCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGCA‑CCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGa                                                                                                                         <  1:895021/239‑1 (MQ=255)
                                                                                               tctcACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAGAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGCA‑CCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGAc                                                                                                                        <  1:1171320/239‑1 (MQ=255)
                                                                                                 tcACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGCA‑CCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGACTg                                                                                                                      <  1:801913/239‑1 (MQ=255)
                                                                                                         aaTCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGAATTCATATTTTGA‑ATCCGCA‑CCCCCCCGGGAAGAGCAGGCACCGCGCCTTTTCGCCCCTTCCACCCCCCCGGCGGCCACCAGCCGGTGGAAATCGACACACCACACCCTCGACCGCGCCTTa                                                                                                               >  2:468483/1‑238 (MQ=255)
                                                                                                                    ccGTCTCTACACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCGGATTGTGGATTCGCCTGGCGAAACGTTACGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGCA‑CCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACcgc‑g                                                                                                  <  2:1455206/239‑1 (MQ=255)
                                                                                                                                                  tGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTTATATTTCGA‑CCCTGCA‑CCCCCCCGGGAAAGCAAGGGCCCGCGCCTTTTCGCCTCTTCCCGCCCCCTGGCGGCAAACAGCTGATGGAACTCGACCAACAACACCCTCCACTGCGCACTAGCAAAAACCCC‑GCTTTTCACCTCCCGCGCCCCccca‑cccgta                                                                   >  2:425599/1‑235 (MQ=255)
                                                                                                                                                      aaTCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGCA‑CCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGC‑GCTTTTCACTTCCGgca                                                                                  <  1:1544400/221‑1 (MQ=255)
                                                                                                                                                       aTCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGTTTCCTATTTTGA‑CCCTCCC‑CCCCCCGGGGTAGGCAAGGTACCCCCCTTTTTGGGCCCTTTCAACCCTCTTGCGGCAATCGGCGCGTTAAACTCCACCATCAAACCCCTCCACTCCGCACTAGCCAAAACCCCTGCTTTGC‑CTTCCGGCACCCCACCACCCGTTaaaaa                                                               >  2:1382075/1‑236 (MQ=255)
                                                                                                                                                                tGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGTTTTATATTTTGA‑CTCTGCA‑CCCACCCGGGAAGGGAAGGGACCGCGCTTTTTCGCCTCTTCCCGCACTCTGGCGGCAAACCGCTGGTGAAAATCGACAATCAACACCTTCCACTGCGCATTAGCAACCACCCC‑GCTTTTTACTTCCCGCACCCCaccc‑cc                                                                       >  2:1544400/1‑221 (MQ=255)
                                                                                                                                                                tGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGCA‑CCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGC‑GCTTTTCACTTCCGGCACCGCACCA‑CCGTTAAGCAAAGCCAGCaa                                                     <  1:158769/239‑1 (MQ=255)
                                                                                                                                                                   tCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGGGTTTATATTTTGA‑CTCTGCC‑CCCCCCGGGGAAAGCAAAGGACCGCGCTTTTTCGCCTCTTCCCGCACTCTGGCGGGAATCGGGTGATGAAACCAGACAAACAACAACTTCCCCAGCGCATTTGCAAACACCCC‑GCCTTTCTCTTCCCGCGCCCCACCA‑CCGCTAAGCAAAGGCACCAAc                                                    >  2:1318605/1‑237 (MQ=255)
                                                                                                                                                                              aTTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGCA‑CCCACCGGGTAAGGCAAGGCACCGCGCTTTTgcgc                                                                                                                                                                                  <  1:116539/102‑1 (MQ=255)
                                                                                                                                                                              aTTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGCA‑CCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGC‑GCTTTTCACTTCCGGCACCGCACCA‑CCGTTAAGCAAAGCCAGCAACCGTTCAGATAcc                                        <  1:5660/238‑1 (MQ=255)
                                                                                                                                                                              aTTCGCCTGGCGAAACGATCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGCA‑CCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGC‑GCTTTTCACTTCCGGCACCGCACCA‑CCGTTAAGCAAAGCCAGCAACCGTTCAGATAcc                                        <  1:356252/238‑1 (MQ=255)
                                                                                                                                                                                       gCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGCA‑CCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGACTgcg                                                                                                                    <  1:553286/155‑1 (MQ=255)
                                                                                                                                                                                        cGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGCA‑CCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACCCCTTCGACTGCGCATTAGCAATCACCGC‑GCTTTTCACTTCCGGCACCGCACCA‑CCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCa                              <  2:93705/238‑1 (MQ=255)
                                                                                                                                                                                        cGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGAGTTTATATTTTTATTTCTCCC‑CCAACCGGG‑GAGGCAAGGGATCGCCCTTTTGCGTCTCTTTCacc                                                                                                                                                                        >  2:116539/1‑100 (MQ=255)
                                                                                                                                                                                                           gATAAAAGAGCGGCGGGATTTCATATTTCGA‑CCCTGCC‑CCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGC‑GCTTTTCACTTCCGGCACCGCACCA‑CCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGgcgc           <  2:711910/238‑1 (MQ=255)
                                                                                                                                                                                                                    gcggcgGGGTTTTATATTTTGA‑CCCTGCC‑CCCCCCGGGGAAGGCAAGGCACCCCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAAACGGCTGATGAAACTCGACAATCAACACCTTCCCCTCCGCCTTAGCAATCACCCC‑GCCTTTCACTTCCCGCACCGCACCA‑CCCTTCAACAAAACCAGCAAACGCTCATATAACCCTCCCCCCACCCCCTGCATCACCGGCGCACACAACAg  >  2:67659/1‑238 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                              
CCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTGGATTCGCCTGGCGAAACGTTCCGGAAAGGCGATAAAAGAGCGGCGGGATTTCATATTTCGA‑CTCTGCA‑CCCACCGGGTAAGGCAAGGCACCGCGCTTTTGCGCCTCTTCCAGCACTCTGGCGGCAATCGGCTGATGAAACTCGACAATCAACACCTTCGACTGCGCATTAGCAATCACCGC‑GCTTTTCACTTCCGGCACCGCACCA‑CCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAG  >  NC_000913/3504857‑3505306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑