Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,757,6680CT32.6% 67.1 / 18.7 43A54T (GCA→ACA) yiaYL‑threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (24/5);  new base T (0/14);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 1.48e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGGTTTTGCAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGT‑GTGGAGAACCACCGCCT‑AAGGAGATCACGCTATCGCAATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGAT  >  NC_000913/3757440‑3757893
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                 
gCGGTTTTGCAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGT‑GTGGAGAACCACCGCCT‑AAGGAGATCACGCTATCGCAATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGGTCCATCATAAATAACGCTAAAAATATTGCGGTCTTCCACGGCTTTTTggc                                                                                                                                                                                                                           >  2:1254350/1‑235 (MQ=255)
                           cGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGT‑GTGGAGAACCACCGCCT‑AAGGAGATCACGCTATCGCAATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAAtt                                                                                                                                                                                                      >  1:463152/1‑231 (MQ=255)
                                          gcgcgccATTGGCCGCCCCCCAGACAATTCCTTTTGCGGAGGTGTGGTGAAGACCCCCCGCAT‑AAGGAGACCACGTTATCGTAATTTTTCTCTTAAAGTAATTTAAAACCTGCGGCGACTTTTTCGGGGGGGGGTTTAGGTTGGGTGCAATAAAAAATAACGCAAAAAATATTGCGTTCTTCCAGTGTTTTTTCCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGAc                                                                                                                                                                                <  2:1328752/236‑1 (MQ=255)
                                          cgccgcCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGT‑GTGGAGAACCACCGCCT‑AAGGAGATCACGCTATCGCAATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACa                                                                                                                                                                               >  1:1372535/1‑239 (MQ=255)
                                              gccATGGGCTCCAACCGGCCAAATCCTTTGTGCGAAGTGGT‑GTGAAAACCCACCGCCT‑AGGGAGATCCCGCTATCGAATTTTTTCTCTTAAAGTAATTCCACCCTGGGGGCGGCTTTTTCCGTGGTGGGGTTAGGTGGGGTGCCATAAAAAATAACGAAAAAAATATTGCTTTCTCCAAGTGTTTTTGCCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTa                                                                                                                                                                           <  2:1070558/239‑1 (MQ=255)
                                                                      ccttttgGCGCTGTCGT‑GTGGAGACCCACCGCCT‑AAGGAGACCACGCTATCGCAATTATTCTCTTAAAGTAATTTCAACCCTGCGGCGATGTTTTCCGTGGGGGGGTTAGGTTGGGTGCCATAAAAAAAAACGCAAAAAATATTGCTTTCTTCCAGTGTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTg                                                                                                                                                   <  2:148144/236‑1 (MQ=255)
                                                                                     gtggtgGAAGACCACCCGCTAAAGAAGATCACTCTTTCAAAATTTTTCTTTTTAATTAATTTCAACCGTGCGGCGACTTTTCCCGGGGTGGGTTTAGGTTGGGTGCCATCAAAAATCACCATAAAAATATTGCGTTTTTCCATTGCTTTTTCCCCATCCCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCata                                                                                                                                           <  2:811045/231‑1 (MQ=255)
                                                                                               acccccggtcaaaGGAGACCACTGTATCGCATTTTTTTTTTTTAAGTAATTCAAACCCGGCGGCGACTTTTTCCGTGGGGGGTTTAGGTTGGGTCCTATCATAAATACCGCAAAAAATTTTGCGTTTTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGt                                                                                                                             <  2:1008881/227‑1 (MQ=255)
                                                                                                   ccGCCT‑AAGGAGATCACGCTATCGCAATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACgg                                                                                                                                                                   >  1:571366/1‑195 (MQ=255)
                                                                                                       cT‑AAGGAGATCACGCTATCGAATTTTTTCTCTTTAAGTAATTTCAACCCTGCGGCGACGTTTTCCGGGGGGGGGTTAGGTTGGGTGCCATCAAAAATAACGCAAAAAATATTGCTTTCTTCCAGTTTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGt                                                                                                                                                                                   <  2:1014593/175‑1 (MQ=255)
                                                                                                                  cACGCTATCGCAATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAg                                                                                                                                                                         >  1:1014593/1‑175 (MQ=255)
                                                                                                                       tATCGCAATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATaa                                                                                                                                                       >  1:274010/1‑188 (MQ=255)
                                                                                                                             aaTTATTCTCTTTAAGTATTTTCAACCCGGCGGCGACTTTTTCCGTGGTGGGGTTGGGTTGGTTGCCATCAAAAATAACGCAAAAAATTTTTCGTTCTTCCAGTGTTTTTTCCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTa                                                                                                                                                                      <  2:137611/167‑1 (MQ=255)
                                                                                                                                       tttAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATc                                                                                                                                                            >  1:137611/1‑167 (MQ=255)
                                                                                                                                       tttAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAgcg                                                                                                                >  1:188214/1‑211 (MQ=255)
                                                                                                                                         tAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGCGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACATTTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAAc                                                                                  >  1:182399/1‑239 (MQ=255)
                                                                                                                                                           cggcgACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATAtt                                                                                                                                         >  1:1003544/1‑166 (MQ=255)
                                                                                                                                                           cggcgACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAac                                                                 >  2:1211097/1‑238 (MQ=255)
                                                                                                                                                                            gtggGGTTAGGTTGGGTTCAATAAAAAATAACGCTAAAAATTTTGCTTTCTTCCAGTGTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCa                                                                                                                               <  2:46824/159‑1 (MQ=255)
                                                                                                                                                                                gtttAGGTTGGGTGCCATCAAAAAAACCGCAAAAAATTTTGCTTTCTTCCAGTTTTTTTTGCACATCGCCCGCCATACCTAAt                                                                                                                                                                                                       <  2:293018/81‑1 (MQ=255)
                                                                                                                                                                                      gTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAAt                                                                                                                     >  1:46824/1‑159 (MQ=255)
                                                                                                                                                                                       ttGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAAt                                                                                                                                                                                                       <  1:101819/76‑1 (MQ=255)
                                                                                                                                                                                       ttGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAAt                                                                                                                                                                                                       >  2:101819/1‑76 (MQ=255)
                                                                                                                                                                                          ggTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACa                                                                                                                                                                                             >  1:293018/1‑83 (MQ=255)
                                                                                                                                                                                          ggTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGt                                                                                                                                                                       >  1:1356546/1‑105 (MQ=255)
                                                                                                                                                                                          ggTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCa                                  >  2:1312236/1‑238 (MQ=255)
                                                                                                                                                                                           gTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCAt                                                                                                                                 >  2:1151114/1‑142 (MQ=255)
                                                                                                                                                                                           gTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCAt                                                                                                                                 <  1:1151114/142‑1 (MQ=255)
                                                                                                                                                                                               aataaaaaataaCGAAAAAATTTTTGCTTTCTCCCGGTGTTTTTTCCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTAc                            <  1:1254350/233‑1 (MQ=255)
                                                                                                                                                                                                 tcatAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATaaa                                                                                      >  2:1240489/1‑179 (MQ=255)
                                                                                                                                                                                                 tcatAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATaaa                                                                                      <  1:1240489/179‑1 (MQ=255)
                                                                                                                                                                                                  caaaaatacCGCTAAAATTATTGCTTTCTTCCAGTCTTTTTTCCCCTTCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCAt                                                                                                                                 <  2:659636/132‑1 (MQ=255)
                                                                                                                                                                                                   aaaaatacCGCAAAAAATTTTGCTTTTTTCCGGTTTTTTTTGCCCATCGCCCGCCATACCTAATTTCg                                                                                                                                                                                                   <  2:227721/66‑1 (MQ=255)
                                                                                                                                                                                                           cGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTaaaa                                             >  1:1551868/1‑210 (MQ=255)
                                                                                                                                                                                                            gCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATAtt                                                                                                                                         >  1:516689/1‑117 (MQ=255)
                                                                                                                                                                                                            gCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGa                                                                                                                       >  1:659636/1‑135 (MQ=255)
                                                                                                                                                                                                             cTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATAtt                                                                                                                                                                                         >  1:227721/1‑68 (MQ=255)
                                                                                                                                                                                                                aaaaTATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAaca                                                                >  1:1002603/1‑186 (MQ=255)
                                                                                                                                                                                                                       tGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATg    >  1:525398/1‑239 (MQ=255)
                                                                                                                                                                                                                       ggattttgccgaGTGTTTTTTGCCCACCCCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATg    <  2:312024/228‑1 (MQ=255)
                                                                                                                                                                                                                          gTTCTTCCAGTGTTTTTTGCACTCCCCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGAt  <  1:784399/238‑1 (MQ=255)
                                                                                                                                                                                                                          gTTCTTCCAGTGTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGAt  <  1:1211097/238‑1 (MQ=255)
                                                                                                                                                                                                                          gTTCGTCCAGTGTTTTTTGCACACGCCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGAt  <  1:1451619/238‑1 (MQ=255)
                                                                                                                                                                                                                                      cTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTg                                                                                                                                                                                  <  1:891461/50‑1 (MQ=255)
                                                                                                                                                                                                                                      cTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTg                                                                                                                                                                                  >  2:891461/1‑50 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                 
GCGGTTTTGCAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGT‑GTGGAGAACCACCGCCT‑AAGGAGATCACGCTATCGCAATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGAT  >  NC_000913/3757440‑3757893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑