Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,429,9900AT35.0% 64.1 / 17.0 40M43L (ATG→TTG) cycAD‑alanine/D‑serine/glycine transporter
Reads supporting (aligned to +/- strand):  ref base A (8/18);  new base T (14/0);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 2.20e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAG  >  NC_000913/4429776‑4430215
                                                                                                                                                                                                                        |                                                                                                                                                                                                                                       
aTGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGGGGCCATTGGATGGGGGTGGTTTTTGGGGTCGGCCAAACGaatta                                                                                                                                                                                                                  >  2:1470270/1‑233 (MQ=255)
        cGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCggg                                                                                                                                                                                                      <  1:995924/239‑1 (MQ=255)
               gATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCCTGGCACGGGGGTTTTTTTGGGGTTTGGCACAACACTTTGCCCTTCCCGGGcctcgt                                                                                                                                                                                                >  1:1114331/1‑235 (MQ=255)
                      cgtATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAtttt                                                                                                                                                                                       <  1:1001280/239‑1 (MQ=255)
                                actgactAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGTCATTGGAACCGGGGTGTTTTTGGGGGCTGGCGAAAAAAGTAGGCATTTCGGGGGGTCGAGACTTTTCGTTTATATg                                                                                                                                                                             >  2:1001280/1‑239 (MQ=255)
                                    actAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTTGTACGGGGGTGTTTTTGGGGTCTGGCAAAACGATTAGCCCTTCCGGGCCGTCGGTCATTTTTGTTTTTTTGTTCa                                                                                                                                                                         >  1:696384/1‑239 (MQ=255)
                                     ctAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTtata                                                                                                                                                                               <  1:1470270/232‑1 (MQ=255)
                                         aGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATTGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGGCGATCATTTTTGTTTATATGATCATTGGt                                                                                                                                                                   >  1:50284/1‑239 (MQ=255)
                                              gTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGGACGGGGGTGTTTTTGGGGTCTGGCAAAACGATTAGCCTTGTCGGGCCGGCGGTCCTTTTCGGTTATATGATAATTTGGTTTAt                                                                                                                                                              >  1:211165/1‑239 (MQ=255)
                                              gTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGGCTGGCAAAAAGATTAGCCTTGCCGGGCGCGGGATCGTTTTTGTTTTTATGGTCATTTGGttttt                                                                                                                                                               >  1:1252155/1‑238 (MQ=255)
                                                agagCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGGAAGGGGGTGTTTTTTGGGGGTGGCAAAACGAATAGCCTTTCCGGGCCGTCGAGCATTTTCGTTTTTATGATTCTTGGGTTTAt                                                                                                                                                              >  1:509647/1‑237 (MQ=255)
                                                     cTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGctct                                                                                                                                                         <  1:1485676/237‑1 (MQ=255)
                                                                      gTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTg                                                                                                                                                                     <  1:361027/208‑1 (MQ=255)
                                                                       tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGGCCTTGGGACGGGGGTGTTTTTGGGGTCTGGCAAAACGAATAGCCTTGCCGGGCCGCCGGGCCTTTTCGTTTTTATTATAATTGGTTTTATTCTCTTTTTCGTGGTTCGGGCAAta                                                                                                                                     >  2:554659/1‑238 (MQ=255)
                                                                       tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCggg                                                                                                                                          <  2:518412/234‑1 (MQ=255)
                                                                       tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCg                                                                                                                                            <  2:973123/232‑1 (MQ=255)
                                                                                          ggTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGGGGCATTGGCTGCGGGGTGTTTTAGGGGCGTGGCCAAAAGATCAGCCCTGCCGCGGCGCCGTTCATTTTCCGTTTTTTGATCATTCGTTTTTTGTTCTTTTTTGTTGTGCGGGCAATGGGGGGATTGCTGCTTTCGa                                                                                                                 >  2:896646/1‑239 (MQ=255)
                                                                                                                    atgatCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCCGCTTATTGCCATTGGCGGTGGCCTTCCGCTGGGGGTGTTTTTGGGGGTTTGGCAAACGATTTCGCCTTCCCGGGCGTCCGTCATTTTTCGTTTTATTGTTCTTTGGTTTTTCGCTTTTTTTGTGTTTGGGGCCAGTGGGGGATTGGTGCCTTTCGTACCTGGATTAACATCtttttatgga                                                                                       >  2:1920/1‑233 (MQ=255)
                                                                                                                       atCAGGCTCCGGCGGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCaa                                                                                                                                                                                                                           <  1:1323985/109‑1 (MQ=255)
                                                                                                                             cTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCt                                                                                 <  1:554659/236‑1 (MQ=255)
                                                                                                                                 ggCTGAACAGTCGCTACGGCGCAATCTCGCAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGAt                                                                                                                                                                           <  1:322228/144‑1 (MQ=255)
                                                                                                                                 ggCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGTACGGGGGTGTTTTTGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTTGTGATGCGGGGAATGGGGGGATTGCTGCTTTTGAATCTGGAATACAAATCTTTTTGTGGCTTCggcttc                                                                              >  2:812618/1‑230 (MQ=255)
                                                                                                                                                  ggCCCAATCTCACAAACCGAAATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTAGGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGCTCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACGCGGGCCGTGGGCa                                                         <  2:1322159/239‑1 (MQ=255)
                                                                                                                                                                     aCATATTCAGCTTATTGCCATTGGCGGGGCCATTTGTACGGGGGTGTTTTTGGGGTCTGGCCAAACGAATAGACCTGCCGGGCCGGCGATCATTTTTGTTTTTATGATTATTGGTTTTTTTCTCTTTTTTGTGATGCGGGCAATGGGGGGATTTGTGCTTTTGTATCTGGTATACAAAACTTTTATTGACTTCGCTTCCGATTTACTCCGGGCGTGGGCAGGGTATTTTACAGGccgt                                       >  2:1559828/1‑235 (MQ=255)
                                                                                                                                                                         atTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAtt                                                                                                                                                                                         <  1:714911/90‑1 (MQ=255)
                                                                                                                                                                               cTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTTTGGGGGCTGGCCAAAAGAATAGGCTTGCCGGGGCGTCGGTCATTTTTGTTTATATGATCATTTGTTTTTTTCTCTTTTTTTTGATGCGGGGAATGGGGGGAATGTTGCTTTCTGATCTTGAATACAAAACTTTTAGTGAACTCGCTTCCGGTTTACTCCGGGCGGGGGGAGGGTATTTTAACGGCCGGGCTTACttggt                            >  2:1602997/1‑235 (MQ=255)
                                                                                                                                                                                ttATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTgg                              <  1:1602997/236‑1 (MQ=255)
                                                                                                                                                                                   ttGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTTTTGGGGCTGGCAAAAAGGTTAGCCCTGCCGGGGCGGCGATCATTTTCTTTtata                                                                                                                                                                               >  2:714911/1‑90 (MQ=255)
                                                                                                                                                                                    tGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATg                                                                                                                                                                                                                                      >  1:190167/1‑37 (MQ=255)
                                                                                                                                                                                      ccATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAt                                                                                                                                                                                          <  1:1550374/76‑1 (MQ=255)
                                                                                                                                                                                      ccATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTg                                                                                                            <  1:1270907/152‑1 (MQ=255)
                                                                                                                                                                                                 tGCCGTTGGTACGGGGGTGTTTATGGGGGCGGGCAAAACGAATAGCCTTGCCGGGCCGGCGGTCTTTTTTGTTTATATGGTCATTGGGTTTATGCTCTTTTTTGTGATGCCGGCAATGGGGGAATTGCTGCTTTCGAATCTTGGATAAAAAACTTTTTGTTACTTCGCTTCCGGTTTTTTCGGGCCGTGGGGAGGAGATTTTTCCGGCGGGGCTTTCTTGGTCTGCTGGGgtgttaacc           >  2:382662/1‑234 (MQ=255)
                                                                                                                                                                                                 tGCCATTTGTACGGGGGTGTTTTTGGGGGCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTTGTTTATATGATCATTGGTTTTTTGGTCTTTTTCGGGATGCGGGCAATGGGGGGATTGCTGCTTTTGAATCTTGGATACAAAACTTTTTGTGACTTCGCTTCCGGTTTTCTCGGGCCGTGGGGAGGATATTTTACCCGCTGGGCTTTATGGGTCTCGTGGGGTGTAACCg           >  2:113016/1‑239 (MQ=255)
                                                                                                                                                                                                            aCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTa               <  2:1195629/225‑1 (MQ=255)
                                                                                                                                                                                                            aCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTa               >  1:1195629/1‑225 (MQ=255)
                                                                                                                                                                                                            aCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGCAACCGGTATGGCAg  >  2:1328423/1‑238 (MQ=255)
                                                                                                                                                                                                                ggTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTg                                        <  2:1088669/196‑1 (MQ=255)
                                                                                                                                                                                                                ggTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTg                                        >  1:1088669/1‑196 (MQ=255)
                                                                                                                                                                                                                ggTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCgg          >  1:481015/1‑226 (MQ=255)
                                                                                                                                                                                                                ggTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCgg          <  2:481015/226‑1 (MQ=255)
                                                                                                                                                                                                                        |                                                                                                                                                                                                                                       
ATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAG  >  NC_000913/4429776‑4430215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: