Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,933,4090TC31.6% 182.4 / 10.0 79E399G (GAA→GGA) edd6‑phosphogluconate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (46/8);  new base C (0/25);  total (46/33)
Fisher's exact test for biased strand distribution p-value = 5.56e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAA‑CAACCGCTGGCGCTTCAATCACCTG‑GTTCTCAACCGGCACGGCAGAGGTTTT‑CATAACCGCACGGCCCAGGTTA‑CCGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCA  >  NC_000913/1933189‑1933642
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                          
accaccacaACCGCGGCCCAGAAACCCCGCTTCAAGGCCCGGCATACGTTCATGC‑GGCTTTAAAAAACCACCCGCTGGCGCTCCAACCCCGTG‑TTTCACACCCGGCACGGCAGGGGTTTT‑AATACCCGCGCGCCCCAGGTTA‑CCGCTTAACCCTTTTGCCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGAAAGCGTTCACTTGGCTGTAGAGTGT‑TTTTTCCGCCCCTCC‑CCGc                                                                                                                                                                                                                             <  2:928786/230‑1 (MQ=255)
                      acaCCCGCTTCAAAGGCCGGCAAAACGTCATGCTGGTTTTAAAAAA‑CCACCGCTGGCGCTCCAATCCCCTG‑GTTCCCACCCGGCACGGAAGAGTTTTT‑CATAACCGCACGGCCCAGGTTA‑CCGTTTAACACTTTTCCCCCACC‑ATGAGGGGAGAAAGGT‑TGTTCGAAGGAAGCGACCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCa                                                                                                                                                                                                   <  2:146793/237‑1 (MQ=255)
                          ccGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAA‑CAACCGCTGGCGCTTCAATCACCTG‑GTTCTCAACCGGCACGGCAGAGGTTTT‑CATAACCGCACGGCCCAGGTTA‑CCGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGt                                                                                                                                                                                               >  2:1374024/1‑239 (MQ=255)
                          ccGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAA‑CAACCGCTGGCGCTTCAATCACCTG‑GTTCTCAACCGGCACGGCAGAGGTTTT‑CATAACCGCACGGCCCAGGTTA‑CCGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGt                                                                                                                                                                                               >  1:931648/1‑239 (MQ=255)
                               tCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAA‑CAACCGCTGGCGCTTCAATCACCTG‑GTTCTCAACCGGCACGGCAGAGGTTTT‑CATAACCGCACGGCCCAGGTTA‑CCGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAg                                                                                                                                                                                          >  1:167932/1‑239 (MQ=255)
                                 aacggcagacATACCGCCATGTCGACTTACAAAAA‑CACCCGCGGGCGATTCAATC‑CCTGTTTTCCCACCCGGCACGGCAGGGGTTTT‑CAAACCCGCCCGGCCC‑GGTTACCCGCTTACCTC‑TTTGTCCCCCCAATGAGGAAAGAAGGGT‑TGTTGGAAGGAAGCGATCACATTGGTGGCGATTGA‑TTTCCCCGCCCGTCC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAggg                                                                                                                                                                                        <  2:74265/230‑1 (MQ=255)
                                       ctgCATACCGTCTTGTTGTTTTTAAAAAC‑CACCCGCTGTCCCTTCAATCCCCTG‑TTTCGCACCCGGCCCGGCGGAGTTTTT‑CATAACCGCACGCCCCATTTTA‑CCGCTAAGCCTTTTGGCCCCCCC‑ATGATGAGAGAAAGGT‑TGTTCAAGGGAAGCGATCACATTGCTGTCGGGTGT‑TTTTCCCCCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATaa                                                                                                                                                                                   <  2:1043871/236‑1 (MQ=255)
                                                           ttaaaaaaa‑CCCCCGCTGCCCTTCCAATGCCCTG‑TTTCACACCCGCCCCGGC‑GTGGTTTTCCAAACCCGCACGGCCCAGTTTC‑CCCCTTAACTCTTTTGCCCCACC‑ATGATGAGAGAAGGTT‑TGTTCGAAGGAAGCGATCACTTTGCTTTCGAGTGT‑TTTTCCCCCCCCTCC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGcc                                                                                                                                                              <  2:219351/235‑1 (MQ=255)
                                                            tcaaaaaa‑CACCCGCTGGCGTTACACTCCCCTG‑GTTCCCACCCGGCACGGCAGAGTTTTT‑CATACCCGCACGGCCAGGGTAA‑CCGCTTAACCTTTTTGCCCCACC‑ATGAGGAGAGAAGGGT‑TGTCCAAGGGAAGCGATCACATTGCTGTGGAGTGA‑TTTTCCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCa                                                                                                                                                             <  2:1299622/236‑1 (MQ=255)
                                                              cAAAAA‑CAACCGCTGGCGCTTCAATGACCTG‑GTTCTCAACCGGCACGGCAGAGGTTTT‑CATAACCGCACGGCCCAGGTTA‑CCGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCAcc                                                                                                                                                           >  1:219351/1‑239 (MQ=255)
                                                                               cttttCAACCCCCTG‑GTTCCCAACGGCCAGGGCGGAGTTTTC‑CAAAACGCCAGGCCCCGGGTTC‑CCGCTAAACCTTTTTCCCCCACC‑AGGATGAGAGAAAGGT‑TGTCCGAAGGAAGCGATCCCTTGGCTGTCGAGTGA‑TTTTCCCCCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCa                                                                                                                                            <  2:1325159/235‑1 (MQ=255)
                                                                                               gTTCTCAACCGGCACGGCAGAGGTTTT‑CATAACCGCACGGCCCAGGTTA‑CCGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCCGGCCt                                                                                                                                >  1:409796/1‑235 (MQ=255)
                                                                                               gTTCTCAACCGGCACGGCAGAGGTTTT‑CATAAACGCACGGCCCAGGTTA‑CCGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGCAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGGATAACGAGACAGACCAAAACCTGCCACCGTATTGACATATTCATGCAGCAGGCCt                                                                                                                                >  2:1131732/1‑235 (MQ=255)
                                                                                                          gcacggcaGAGGTTTT‑CATAACCGCACGGCCCAGGTTA‑CCGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTc                                                                                                                  >  2:342559/1‑238 (MQ=255)
                                                                                                                caGAGGTTTT‑CATACCCCCCCGGCCAGGGTTC‑CCGCTTAACCCTTTTGTCCCACC‑ATGATGAAAGAAGGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGGGA‑TTTTCCCCCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACgaga                                                                                                                                                                              <  2:373669/172‑1 (MQ=255)
                                                                                                                        ct‑CAAAACCGCGGGGCCCTGTTCA‑CCGCTAAACGTTTTTGCCCCCCG‑ATTAGGAGAGAAGGGT‑TGTACGAAGAAAGCGACCACATTGTTGTGGGGTTT‑TTTTCCCCCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCa                                                                                                                                                                                            <  2:1494913/149‑1 (MQ=255)
                                                                                                                         tccaaacccGCGCGGCCAAGTTTC‑CCGCTAAACGCTTTGTCCCCACG‑AGGA‑GAAAGAAAGTTGTTGTAGAGGGAGGCCATCTCTTTGCTGTCGGTTGT‑TTTCCCCCCCCCTTC‑CCGCCAGTCCAGTTCCCCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACC‑AAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGt                                                                                                  <  2:1589011/232‑1 (MQ=255)
                                                                                                                           cATAACCGCACGGCCCAGGTTA‑CCGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCaaaa                                                                                                                                                                    >  1:373669/1‑172 (MQ=255)
                                                                                                                                 cGCACGGCCCAGGTTA‑CCGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCa                                                                                                             >  1:1178330/1‑221 (MQ=255)
                                                                                                                                   cACGGCCCAGGTTA‑CCGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAAc                                                                                                                                                                                  >  1:1494913/1‑150 (MQ=255)
                                                                                                                                   cACGGCCCAGGTTA‑CCGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCa                                                                                            >  1:1253797/1‑236 (MQ=255)
                                                                                                                                        cccGGGTTC‑CCTCTTACCATTTTTCCCCCACA‑TTGATGAGAAAAAGTT‑TGTCCAAAGTAAGCCATCACTTTGCTGCCGAGTAT‑TTTTCCCCCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCa                                                                                                                                                                                                   <  2:540961/128‑1 (MQ=255)
                                                                                                                                        cccAGTTTC‑CCGCTTAACCCTTTTGTCCCCCC‑ATGATGAGAAAAAGTT‑TTTTCGAAGGAAGCGATCACTTTGCTGTGGATTAT‑TTTTCCCCCCCCTTC‑CCGCCAGTCCAGtt                                                                                                                                                                                                                   <  2:1210916/112‑1 (MQ=255)
                                                                                                                                        cccAGGTTC‑CCGCTTAACATTTTTGCCCCCCC‑ATGATGAGAAAAAGGT‑TGTTCAAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTCCCCCCCCTCC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGtt                                                                                                                                                                                              <  1:849438/133‑1 (MQ=255)
                                                                                                                                        cccAGGTTC‑CCGATAAACTCTTTTGCCCCACA‑AGGATGAGAGAAAGTT‑TGTTCGAAGGAAGCGATCACATTGCTTTCGGGTGA‑TTTTCCCCCCCCTCC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGtt                                                                                                                                                                                              <  2:1547003/133‑1 (MQ=255)
                                                                                                                                                   cGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGc                                                                                                                                                                                                                                        <  2:241579/82‑1 (MQ=255)
                                                                                                                                                   cGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGc                                                                                                                                                                                                                                        >  1:241579/1‑82 (MQ=255)
                                                                                                                                                   cGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCattatt                                                                                                                                                                                                         >  1:1210916/1‑112 (MQ=255)
                                                                                                                                                   cGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCa                                                                                                                                                                                                   <  1:566886/118‑1 (MQ=255)
                                                                                                                                                   cGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCa                                                                                                                                                                                                   >  2:566886/1‑118 (MQ=255)
                                                                                                                                                   cGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAgg                                                                                                                                                                                         >  1:540961/1‑128 (MQ=255)
                                                                                                                                                   cGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGtata                                                                                                                                                                                    >  2:849438/1‑133 (MQ=255)
                                                                                                                                                   cGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGtata                                                                                                                                                                                    >  1:1547003/1‑133 (MQ=255)
                                                                                                                                                   cGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGAc                                                                                                                                                   >  1:163233/1‑166 (MQ=255)
                                                                                                                                                   cGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTgg                                                                          >  2:1483259/1‑239 (MQ=255)
                                                                                                                                                   cGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTgg                                                                          >  1:1255916/1‑239 (MQ=255)
                                                                                                                                                     cTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGtt                                                                        >  1:1208042/1‑239 (MQ=255)
                                                                                                                                                        aaCACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTATGCCACCTGCCGCCTGGAAGTGGTTAat                                                                     >  1:699018/1‑239 (MQ=255)
                                                                                                                                                          ctcTTTTGCCCCACC‑ATGATGAGAGAAAGGT‑TTTTGGAAGGAAGCGATCACTTTGCTGTCGGGTAA‑TTTTCCCCCCCCTTC‑CCGccagtc                                                                                                                                                                                                                        <  2:740798/88‑1 (MQ=255)
                                                                                                                                                             ttttGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCCCATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCACAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCg                                                                 >  2:1226673/1‑238 (MQ=255)
                                                                                                                                                                  tCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCgg                                                            >  1:485335/1‑238 (MQ=255)
                                                                                                                                                                    ccacc‑aTGATGAGAGAAAGTT‑TGTTCGAAGGAAGCGATCACTTTGCTGTGGAGTTT‑TTTTCCCCCCCCTCC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTc                                                                                                                                                                                             <  2:684162/107‑1 (MQ=255)
                                                                                                                                                                    ccacc‑aTGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCa                                                                                                                                                                                                              >  1:740798/1‑90 (MQ=255)
                                                                                                                                                                    ccacc‑aTGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGAcc                                                         >  1:1120566/1‑239 (MQ=255)
                                                                                                                                                                       cc‑aTGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGt                                                       >  1:1259842/1‑238 (MQ=255)
                                                                                                                                                                       cc‑aTGAGCAGAGAAGGTT‑TGTTCGAAGGAAGCGACCACTTTGGTGTCGTGTTT‑TTTTCCCCCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGtt                                                      <  2:1255916/239‑1 (MQ=255)
                                                                                                                                                                        ccttgatgaGAAAAAGTT‑TTTTCGAAGGAAGCGATCACTTTGCTTTCGAGTGT‑TTTTCCCCCCCCTCC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCa                                                                                                                                                                       <  2:1242187/123‑1 (MQ=255)
                                                                                                                                                                              tgaGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACgaga                                                                                                                                                                              >  2:268623/1‑113 (MQ=255)
                                                                                                                                                                              tgaGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACgaga                                                                                                                                                                              <  1:268623/113‑1 (MQ=255)
                                                                                                                                                                               gagagaAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATaa                                                                                                                                                                                   >  1:684162/1‑107 (MQ=255)
                                                                                                                                                                                  agaAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCa                                                                                                                                                             >  1:1242187/1‑126 (MQ=255)
                                                                                                                                                                                             ttCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCa                                  >  2:117169/1‑239 (MQ=255)
                                                                                                                                                                                                  aggaagCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGtt                                                                                                                                                                                                                   >  2:474174/1‑57 (MQ=255)
                                                                                                                                                                                                  aggaagCGATCACATTGCTGTCGAGTGA‑TTTTCCCGCCCCTTC‑CCGCCAGTCCAGtt                                                                                                                                                                                                                   <  1:474174/57‑1 (MQ=255)
                                                                                                                                                                                                    gactcacgcacaTTGCTGTAGAGTGT‑TTGGTCCGCCCCTTC‑CCGCCAGTCCAGTCCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCCCCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTa                            <  2:409796/230‑1 (MQ=255)
                                                                                                                                                                                                    gaagCGATCACATTGCTGTCGAGTGA‑TTTTTCTGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGCTCAAGGGCATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGATCGGGCAGAGACGAGCCATAAGCGGGac                           >  2:119609/1‑239 (MQ=255)
                                                                                                                                                                                                    gaagCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCa                                                                                                                 <  1:89457/153‑1 (MQ=255)
                                                                                                                                                                                                    gaagCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCa                                                                                                                 >  2:89457/1‑153 (MQ=255)
                                                                                                                                                                                                      agCCATCCCAGTGCTGT‑GAGTTACTTTTCCCCCCCCTCC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTac                           <  2:316104/237‑1 (MQ=255)
                                                                                                                                                                                                       gCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGCCGTGCCATCAGCGGTacaac                        >  1:885509/1‑239 (MQ=255)
                                                                                                                                                                                                       gCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGGGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTacaac                        >  2:19076/1‑239 (MQ=255)
                                                                                                                                                                                                            cactTTGCTGTGGAGTGT‑TTTTCCCCCCCCTTC‑CCGCCAGTCCAGTTCCCCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAg                   <  2:1208042/239‑1 (MQ=255)
                                                                                                                                                                                                               tttGCTGTCGTTTTT‑TTTTCCCCCCCCTTC‑CCGCCAGTCCAGTTCACCattatt                                                                                                                                                                                                         <  2:753049/53‑1 (MQ=255)
                                                                                                                                                                                                               tttGCAGTCGCGTGT‑TTTTCCATCCCCTAC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGtt                                                      <  1:999091/200‑1 (MQ=255)
                                                                                                                                                                                                               gttGCTGTAGGGTGT‑TTTTCCCCCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAg                                 <  2:314382/221‑1 (MQ=255)
                                                                                                                                                                                                               aTTGCTGTCGGTTGT‑TTTTCCCCCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCa                                                                                                                                                                                                   <  2:370535/60‑1 (MQ=255)
                                                                                                                                                                                                                  gCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAgg              >  1:1534393/1‑238 (MQ=255)
                                                                                                                                                                                                                   cTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCa                                                                                                                                                                                                              >  2:1458632/1‑45 (MQ=255)
                                                                                                                                                                                                                    tGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAgg                                                                                                                                                                                         >  1:652957/1‑65 (MQ=255)
                                                                                                                                                                                                                      tCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCa                                                                                                                                                                                                   <  1:1153100/53‑1 (MQ=255)
                                                                                                                                                                                                                      tCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCa                                                                                                                                                                                                   >  2:1153100/1‑53 (MQ=255)
                                                                                                                                                                                                                      tCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAAAATCAGAAAGGCCaga         >  1:244296/1‑239 (MQ=255)
                                                                                                                                                                                                                        gAGTGA‑TTTGCCCGCCCCTCC‑CCGTCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAgaga       <  2:931648/239‑1 (MQ=255)
                                                                                                                                                                                                                         cgtatcttttttCCCCCCTTCACCGCCAGTCCAGTTCACCATTATTCGGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCaga         <  2:485335/232‑1 (MQ=255)
                                                                                                                                                                                                                         aGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGt                                                                                                                                                                                               >  1:753049/1‑54 (MQ=255)
                                                                                                                                                                                                                         aGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAgg                                                                                                                                                                                         >  1:370535/1‑60 (MQ=255)
                                                                                                                                                                                                                         aGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTTAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCCGGAAGTGGTTAATATCGGCCTGACCGTCTGGGCAgaga                                            >  2:999091/1‑201 (MQ=255)
                                                                                                                                                                                                                         aGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGGacaaca                       >  1:314382/1‑222 (MQ=255)
                                                                                                                                                                                                                           tGA‑TTTTCCCGCCCCTTC‑CCGACAGTCCAGTTCACCAGTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGATGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAg     <  2:1255570/238‑1 (MQ=255)
                                                                                                                                                                                                                             a‑TTTTTCCGCCCCTTC‑CCGCAAGTCCCGTTCACCATTATTCAGCCATGGTTCAAGGTCATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCGTTGAGCAGTTCACGCACCAGAACCGGTACGCCCCCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGGAGAGACGTGCCATCAGCGGCACAACATCAGAAAGGCAAGAGAAGTCa  >  2:1223843/1‑239 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                          
AACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAA‑CAACCGCTGGCGCTTCAATCACCTG‑GTTCTCAACCGGCACGGCAGAGGTTTT‑CATAACCGCACGGCCCAGGTTA‑CCGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGT‑TGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGA‑TTTTTCCGCCCCTTC‑CCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTGCCACCGTATTGACATCTTCATGCAGCAGGCCTGCTTTGAGCAGTTCACGCACCAGAACCGGTACGCCACCTGCCGCCTGGAAGTGGTTAATATCGGCCGGACCGTTCGGGTAGAGACGTGCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCA  >  NC_000913/1933189‑1933642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑