New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 4466134152 (1.060)3 (0.020) 3/328 NT 2.1% coding (47/1422 nt) treB trehalose‑specific PTS enzyme: IIB and IIC component
?NC_000913 = 4466205 136 (0.970)intergenic (‑25/+94) treB/treR trehalose‑specific PTS enzyme: IIB and IIC component/trehalose 6‑phosphate‑inducible trehalose regulon transcriptional repressor
Rejected: Frequency below/above cutoff threshold.

TGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4465906‑4466134
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑accaAAAATGTCATCTGCCATGGGGCTTTATGATGAGCAAAATAAACCAAACGGATATCGATCGGTTGATTGAACTGGTCGGCGGGCGCGGCAATATTGCGACGGTG  <  NC_000913/4466205‑4466103
                                                                                                                                                                                                                                                                                                                                            
TGCTTTTTATCACCCTCCTCCTTTCCGGTTGACGCAATCAGTGCTGGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTAAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGAC                                                                                                           <  2:1775015/227‑1
TGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGAC                                                                                                           <  2:1855022/227‑1
                        CCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCTGCGCCCGCCGA                                                                                                            >  1:427753/1‑202
                         CGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGAC                                                                                                           <  2:427044/202‑1
                                                         CCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGA                                                                                                            >  2:873070/1‑169
                                                          CCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGAC                                                                                                           <  1:874593/169‑1
                                                             CGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACC                                                                                                          >  2:401068/1‑167
                                                              GTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCA                                                                                                         <  1:401732/167‑1
                                                               TTGGTGCCAATCACCACCTTAAATTGCCCGGCATTGGTGAAACAGCCTTTCCCCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAAAAATGTCATCTGCCATGGGGCTTTATGATGAGCAAAATAAACCAAACGGATATCGATCGG                                            <  1:124346/227‑1
                                                                                       TGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGA                                                                                                            >  2:1125215/1‑139
                                                                                        GCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGAC                                                                                                           <  1:1127239/139‑1
                                                                                                             CTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGA                                                                                                            >  1:536956/1‑117
                                                                                                              TTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGAC                                                                                                           <  2:536075/117‑1
                                                                                                                                           TCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGA                                                                                                            >  1:1931729/1‑87
                                                                                                                                            CGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGAC                                                                                                           <  2:1928301/87‑1
                                                                                                                                                                    GAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAAAAATGTCATCTGCCATGGGGCTTTATGATGAGCAAAATAAACCAAACGGATATCGATC                                              >  2:1754318/1‑124
                                                                                                                                                                     AGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAAAAATGTCATCTGCCATGGGGCTTTATGATGAGCAAAATAAACCAAACGGATATCGATCG                                             <  1:1757420/124‑1
                                                                                                                                                                                                                                 ACCAAAAATGTCATCTGCCATGGGGCTTTATGATGAGCAAAATAAACCAAACGGATATCGATCGGTTGATTGAACTGGTCGGCGGGCGCGGCAATATTGCGACGGT   >  2:341576/1‑106
                                                                                                                                                                                                                                  CCAAAAATGTCATCTGCCATGGGGCTTTATGATGAGCAAAATAAACCAAACGGATATCGATCGGTTGATTGAACTGGTCGGCGGGCGCGGCAATATTGCGACGGTG  <  1:342152/106‑1
                                                                                                                                                                                                                                                                                                                                            
TGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4465906‑4466134
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑accaAAAATGTCATCTGCCATGGGGCTTTATGATGAGCAAAATAAACCAAACGGATATCGATCGGTTGATTGAACTGGTCGGCGGGCGCGGCAATATTGCGACGGTG  <  NC_000913/4466205‑4466103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.