Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 811,721 T→G 23.0% V67G (GTG→GGG)  bioC → malonyl‑ACP O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913811,7210TG23.0% 124.5 / 2.3 61V67G (GTG→GGG) bioCmalonyl‑ACP O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (11/32);  new base G (14/4);  total (25/36)
Fisher's exact test for biased strand distribution p-value = 3.90e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTACCACGCTGGT  >  NC_000913/811496‑811946
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   
ttccGACCTTCGGGTGGAGGTGCTTCAGGGCACCGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGa                                                                                                                                                                                                                                   <  2:175217/225‑1 (MQ=255)
tatgcaCCGTCTGCTGGAGGTGCTGCATGGCAACGTTAAAGAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGa                                                                                                                                                                                                                                   <  1:307542/223‑1 (MQ=255)
 atCGACCGTCAGCAGGGGTTGCTGCAGGGCAACGTTTAATACACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACCCCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGAc                                                                                                                                                                                                                                  <  2:1255578/228‑1 (MQ=255)
  tCGAACGTGTTCTGGAGGTGCTGCATGGCAACGGTTAAAAAACAAGCCATTGCAGCGGCATTGGGTCGGGCAGCCGCACCCTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGAc                                                                                                                                                                                                                                  <  1:1124479/227‑1 (MQ=255)
        gTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAAAGTCACTCGCCGGTGAc                                                                                                                                                                                                                                  >  2:148014/1‑221 (MQ=255)
                             gCAACCGTTAAAAAACCACGCCGTGGCAGCGGAATGTGGTCGGGCAGCCCCCCACTATGAGCAACGTGCAGTTCTACAGCGCGAGAGTGCTGACGCCTTACGGGCAATGGTTCCACAGCGTAAATCCACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGc                                                                                                                                                                                                        <  2:701446/227‑1 (MQ=255)
                                agggTTAAAAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGCCGCCTTACGGGCAATGCTTCCACAGCGTAAATACCCCCACGTACTGGACGCGGGTTGTGGCCCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGt                                                                                                                                                                                                    <  2:1972123/225‑1 (MQ=255)
                                aCGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCACGCGCAGGGGACGGCCCTAGATACCTCTACGCCaacgactgtt                                                                                                                                                                                                   >  2:1152312/1‑220 (MQ=255)
                                      aataaACAAGCCATTGCAGGGGCATTTGGTCGGGCCGCCGCCCACTATGAGCAACATGCAGATCTACAGGGCAAGATGGCTGACGCCTTACTGGAAATGCTTCCACAGCGTAAATACCCCCACGTACTGGACGCGGGTTGTGGACCTGGCGGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCa                                                                                                                                                                                             <  1:1602456/228‑1 (MQ=255)
                                               aGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGACGGCACGCGCAGGGGACGGGCCTAGAGTTCTCGCCCCCACTGCTTGTTCCGGCAAGcc                                                                                                                                                                                         >  1:462081/1‑224 (MQ=255)
                                                          cGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCCGGGGACGGCCCTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCCGGAGGATgac                                                                                                                                                                              >  1:1324952/1‑222 (MQ=255)
                                                             cATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCa                                                                                                                                                                           <  1:1422658/224‑1 (MQ=255)
                                                                      gggCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTAt                                                                                                                                                                  <  1:1409680/224‑1 (MQ=255)
                                                                       ggCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGGAGGGGACGGCCCTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGCAGGATGCCGCAGACCCTTATCTgg                                                                                                                                                              >  1:519073/1‑227 (MQ=255)
                                                                             ctcccattATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACGGGCAATGCTTCCACAGCGTAAATCCACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGgaga                                                                                                                                                      <  1:1081619/221‑1 (MQ=255)
                                                                                  acTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACGGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGGAAGGCACGGGGAGGGGAGGGGCTTAGATCTCTCGCCGCCGACGCTTGTTTCTGGACGGCAGAAGGATGGCGCAGGCCCTTTAATGGCCGGGAGATATc                                                                                                                                                  >  2:34165/1‑228 (MQ=255)
                                                                                    tATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGa                                                                                                                                                                                                                                   >  2:1154428/1‑145 (MQ=255)
                                                                                     aTGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGAc                                                                                                                                                                                                                                  <  1:1156498/145‑1 (MQ=255)
                                                                                       gAGCAAAATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGAc                                                                                                                                                                                                                                  <  1:688762/143‑1 (MQ=255)
                                                                                              aTGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCCCCCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTTTTCAGGCCCGCCCGAAGGATgtc                                                                                                                                                                              >  2:1241539/1‑186 (MQ=255)
                                                                                               tGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCg                                                                                                                                                                             <  1:1243761/188‑1 (MQ=255)
                                                                                                               cAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACgg                                                                                                                                                                                                                                <  1:1428011/121‑1 (MQ=255)
                                                                                                                agagTGCTGACGCTTTACGGGAAGTGCTCCAACCGTGAAAACCCCCCCAGGTCGGGGCCGGGGTTTGGGGCCCGGGGGGGATGGCCCCCCCGGGGCGGAAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGtt                                                                                                                     <  2:518224/226‑1 (MQ=255)
                                                                                                                    tGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCACGCGCCGGGGGCGGCCTTAGATCTCTCGCCGCCAATGCTTGTTTAGGCACGCCAGCAGGATGCCGcc                                                                                                                                                                           >  2:122040/1‑168 (MQ=255)
                                                                                                                     gCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGCCCTGGCTGGATGGGCCGCCACTGGCGGGAGCGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAg                                                                                                                                                                          <  1:122256/169‑1 (MQ=255)
                                                                                                                      cTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGGTATCCAATCCCCGCCGTTCGCGACCGCGAAGTTCGATCtt                                                                                                              >  2:659528/1‑227 (MQ=255)
                                                                                                                                       aTGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCCGGGGACGGGCTTTGATCTCTCGCCGCCAATGCTTGTTCAg                                                                                                                                                                                                >  2:1695072/1‑129 (MQ=255)
                                                                                                                                        tGCTTCCACAGCGTAAATACCCCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAgg                                                                                                                                                                                               <  1:1698071/129‑1 (MQ=255)
                                                                                                                                        tGCTCCCACGGCTTAAATACCCCGAGGTACTGGCCGCGGGTTGGGGACCTGGCGGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCgca                                                                                            <  1:1154379/227‑1 (MQ=255)
                                                                                                                                                   cGTAAATACACCCAGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGGACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGGGGTATCGAATACCTGCCCTTAGCGACTGCGCCGTTTGGACTTGCATGGGGGACTCCTGCAGTGCAGTGGGGc                                                                                >  2:353819/1‑227 (MQ=255)
                                                                                                                                                    gTAAATACCCCAACGTCCGGGACGCGGGTGGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCg                                                                               <  1:1153539/228‑1 (MQ=255)
                                                                                                                                                     tAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGa                                                                                                                                                                                                                                   >  2:1533762/1‑80 (MQ=255)
                                                                                                                                                      aaaTACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGAc                                                                                                                                                                                                                                  <  1:1536496/80‑1 (MQ=255)
                                                                                                                                                             cccACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTg                                                                                                                                                                                                                                    >  2:723361/1‑71 (MQ=255)
                                                                                                                                                             cccACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCACGCGCAGGGGACGGCCCTTGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCCGAAGGATGcc                                                                                                                                                                              >  2:1284663/1‑125 (MQ=255)
                                                                                                                                                              ccgcgtccgggACGCGGGTTGTGGACCTGGTTGGATGGCCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCgcagtgcagt                                                                                     <  1:610570/203‑1 (MQ=255)
                                                                                                                                                              ccACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGa                                                                                                                                                                                                                                   <  1:724591/71‑1 (MQ=255)
                                                                                                                                                              ccACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCg                                                                                                                                                                             <  1:1286965/125‑1 (MQ=255)
                                                                                                                                                               cACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGGTTGTTCAGGGACGCCAGAAGGGTGCCGCCGACCCTTATCTGGCGGGGGGTATCGAAAcccc                                                                                                                                          >  2:1590818/1‑157 (MQ=255)
                                                                                                                                                                aCGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCCCGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTg                                                                                                                                         <  1:1593647/158‑1 (MQ=255)
                                                                                                                                                                 cGTACGGGACGGGGGTTGTGGACCGGGCTGGATGAGCCGCCACTGGCGGGACCGTCACGCGCAGGTGACGGCCTTAGACCTCTCGCCGACAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa                                                                   <  1:1570092/227‑1 (MQ=255)
                                                                                                                                                                   ctccgtgcgcgGGGTGTAGGGCCCGGCTGGATGTACCCACCTCGGGGGGCATCGTCACGGGCAGGGCACGGCCTTAGATCTCTCGCCGCCAGTGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGCCCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCa                                                                   <  2:1446862/220‑1 (MQ=255)
                                                                                                                                                                      cgggccggggTTTGGGACCGGGGTGGGTGGGCCCGCCAGCGGCGGAAACGTCGCGCGAAGGGAACGGCCTTAGATCTCCCGCCGCCAAGGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGCCCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCa                                                              <  2:1923670/221‑1 (MQ=255)
                                                                                                                                                                                           ggctggATGAGCCGCCACTGGCGGGAACGGCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGGGATATTGAAACCCTGCCGTTAGCGACCGCGACGt                                                                                                                      >  2:1208839/1‑151 (MQ=255)
                                                                                                                                                                                            gctggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGc                                                                                                                                                                            >  1:1250558/1‑97 (MQ=255)
                                                                                                                                                                                            gcgggATGAGCCCCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGtt                                                                                                                     <  1:1210998/148‑1 (MQ=255)
                                                                                                                                                                                             ctggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCa                                                                                                                                                                           <  2:1248330/97‑1 (MQ=255)
                                                                                                                                                                                                gggtggacccgccACTGGGGGAACCGTCCCGCGAAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTACAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGgtggtg                                     <  1:1245025/220‑1 (MQ=255)
                                                                                                                                                                                                   taggcccgccACGTGCGGAAACGTCACGCGCAGGTGGCGGCCTTAGCTCTTGCGCCGCCAATGCTTGTTCAGGCCCGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATTTCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTgcg                                   <  1:1386584/222‑1 (MQ=255)
                                                                                                                                                                                                        ccgccACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGt                                                                                                                      >  1:1629017/1‑140 (MQ=255)
                                                                                                                                                                                                         cgccACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGtt                                                                                                                     <  2:1626137/140‑1 (MQ=255)
                                                                                                                                                                                                            cACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCCGAAGGATGCCGCAGACCCTTATCTGGCGGGGGATATCGAATCCCCGCCGTTTGCGGCTGCGACgg                                                                                                                      >  2:1475004/1‑135 (MQ=255)
                                                                                                                                                                                                            cACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCCCCGCCCATGCTTGTTTAGGGACCCCAGAAGGATGGCGCAGGCCCTTATTTTGCGGGGGGTATCGAAACCCTGCCGTTAGCGGCCGCGACGGTCGATCTTTCAAGGAGGAAACTCGCAGGGGAGTGGGGCGGGGATTTATCCACCGCACTCCGCGAGCCGGATCCGGTGGAGGGGCCCCAAAgc                          >  2:184421/1‑227 (MQ=255)
                                                                                                                                                                                                             aCTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCGCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGtt                                                                                                                     <  1:1477636/136‑1 (MQ=255)
                                                                                                                                                                                                                           cACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCgcagt                                                                                          >  1:1479227/1‑149 (MQ=255)
                                                                                                                                                                                                                           cACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCgg                                                                              >  2:1947948/1‑161 (MQ=255)
                                                                                                                                                                                                                            ccgcgcgGGGGACGGCTTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCGGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCgcagtg                                                                                         <  2:1476592/148‑1 (MQ=255)
                                                                                                                                                                                                                            aCGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGt                                                                             <  1:1951417/161‑1 (MQ=255)
                                                                                                                                                                                                                               cgcAGGTGACGCATTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGAGCCCGCAGCCCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGGGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTACCACg       <  1:1559227/227‑1 (MQ=255)
                                                                                                                                                                                                                                   ggTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGGAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTACCACGCTGGt  >  1:1308351/1‑228 (MQ=255)
                                                                                                                                                                                                                                    gTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCgc                                                                                             >  1:1847917/1‑137 (MQ=255)
                                                                                                                                                                                                                                     tGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCgca                                                                                            <  2:1844637/137‑1 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   
TATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAGCGACTGCGACGTTCGATCTTGCATGGAGCAATCTCGCAGTGCAGTGGTGCGGTAATTTATCCACGGCACTCCGCGAGCTGTATCGGGTGGTGCGCCCCAAAGGCGTGGTCGCGTTTACCACGCTGGT  >  NC_000913/811496‑811946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: