Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,297,688 T→C 10.0% M151V (ATG→GTG)  yraQ ← putative inner membrane permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,297,6880TC10.0% 170.7 / 2.3 62M151V (ATG→GTG) yraQputative inner membrane permease
Reads supporting (aligned to +/- strand):  ref base T (36/19);  new base C (2/5);  total (38/24)
Fisher's exact test for biased strand distribution p-value = 9.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACC  >  NC_000913/3297463‑3297911
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                               
tCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGGTTAACACCGGATTGACCATc                                                                                                                                                                                                                                >  1:1293830/1‑227 (MQ=255)
  catgggccccGGCCCCCGCGGCTAAAAAACCCGCCCTGTACTACCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAg                                                                                                                                                                                                                             <  2:1435982/221‑1 (MQ=255)
    tAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGaa                                                                                                                                                                                                                           <  1:707148/228‑1 (MQ=255)
            tGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGACTGCCAgcg                                                                                                                                                                                                                   >  1:1576531/1‑228 (MQ=255)
              ccccAGCGGCTAAACAACCCGCCCGGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCa                                                                                                                                                                                                                 <  1:702097/228‑1 (MQ=255)
              ccccAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGGATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATCAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCACGAAGCCAgcgc                                                                                                                                                                                                                  >  1:1702821/1‑227 (MQ=255)
                   gCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGcc                                                                                                                                                                                                            <  1:1961339/228‑1 (MQ=255)
                     ggCTAAACACCCCGCCCTGTGCTTCCGGTATTTCAATTACGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGccc                                                                                                                                                                                                           <  2:1501191/227‑1 (MQ=255)
                        tAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCCAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGCTTAACACCGGATTGCCCACCCAGAATGCCAGCGCACCGCCCATc                                                                                                                                                                                                        >  1:1410673/1‑227 (MQ=255)
                            acaCCCGCCCGGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGTCAGCGCACCGCCCATCGacac                                                                                                                                                                                                   <  1:901044/226‑1 (MQ=255)
                                             ccGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTc                                                                                                                                                                                  >  1:785037/1‑228 (MQ=255)
                                                  atgtaagtgtCGACGGGTGCATGGCTTTGCGTTTTTACACGCACCCATTTTGCCCCCGGCGCCGCAACAAGCAACACCATCCCCAGCCCGGCCACCAGACGAACCGCCGCAAAACCCCAGCCGAGGAAAAAGCCAAAAAACACCAGCGCCGCCGGGTTTACCCCCGGATTGCCCACCCAGAATCCCAGCGCCCCGCCCATCGACACCTGTTGGCGACGCATTCccgcc                                                                                                                                                                             <  2:1824857/219‑1 (MQ=255)
                                                       acttgcgcgcGGTGCCTCGTTTTGCGGTTTTCCACCCCCCCATTTTTGCCCACGCCTCACAACCAGCACCACCATCACCAGCCCGGCCACCAGACGAACCGCCGCAAACCCCCAGCCGAGGACAAAGCCCAAAAACCCCAGCGTCGCCGGGTTTAACCCCGGATTCCCCATCCAAAATGCCAGCGCCCCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGAc                                                                                                                                                                        <  2:783709/219‑1 (MQ=255)
                                                         tttCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGAcc                                                                                                                                                                       >  1:137848/1‑227 (MQ=255)
                                                          cccGACCGGGACCTCCGTTTGCGTTGTTTCACGCACCCATTTTTGCCCCAGCGTCGCAATCAGCAACCCCAGCACCAGCCCGGCCACCAGACGAATCGCCGAAAACCCCCAGCCGAGGACAAAGCCCATAAACCCCAGCGTCGCCGGGTTTAACCCCGGATTGCCCATCCAGAATGCCGGCGCCCCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCgg                                                                                                                                                                     <  2:1573734/226‑1 (MQ=255)
                                                              aCCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGc                                                                                                                                                                  >  2:199473/1‑227 (MQ=255)
                                                                   tGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCCGTTGGCGACGCATTCCCGCCGCGACCGGATCCGcac                                                                                                                                                             >  1:553761/1‑227 (MQ=255)
                                                                             tGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCACCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTaca                                                                                                                                                   >  1:389289/1‑227 (MQ=255)
                                                                             tGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGCCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCCGAGCCGCACAGCAGGTaca                                                                                                                                                   >  1:921524/1‑227 (MQ=255)
                                                                                ggTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCCCGACCGGAGCCGCACAGCCGGTACAcat                                                                                                                                                >  2:388877/1‑227 (MQ=255)
                                                                                         cGCCCCCATTTTGCCACCAGGGTCGAACTCAGCACCCCCATCCCCAGCCCGCCCACCAGACGACTCGCCGCAAACCCCCAGCCGAGGACAACGCCCAAAACCCCCAGCGTCGCCGGGTTTACCACCGGATTGCCCATCCAGAATGCCAGCCCCCCGCCCACCGACCCCTGTTGGCGCCGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGc                                                                                                                                      <  2:140187/228‑1 (MQ=255)
                                                                                            aCCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGa                                                                                                                                                                    >  1:466452/1‑195 (MQ=255)
                                                                                                                                 tCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGACAACAGCGTTCCCAGCAGCGTGCCGCGAAAGcgcg                                                                                              >  1:301374/1‑228 (MQ=255)
                                                                                                                                        cccGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGca                                                                                                                                                              >  1:1164494/1‑157 (MQ=255)
                                                                                                                                         ccGGCCACCAGACGAACCGCCGCAAACCCCCAGCCGAGGACAAAGCCCAAAAACCCCAGCGTCGCCGGGTTTAACCCCGGATGGCCCACCCAGAATGCCAGCGCCCCGCCCATCGACCCCTGTGGGCGACGCATTCCCGCCGCGACCGGAGCCGcac                                                                                                                                                             <  2:1162406/157‑1 (MQ=255)
                                                                                                                                          cGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCAc                                                                                                                                                                                                                >  2:1382651/1‑105 (MQ=255)
                                                                                                                                           ggCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCAcc                                                                                                                                                                                                               <  1:1385135/105‑1 (MQ=255)
                                                                                                                                            gCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAg                                                                                    >  1:155587/1‑227 (MQ=255)
                                                                                                                                            gCCACCAGACGAACCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCACCGCACCGCCCATCGACACCTGTTGCCGACGCATTCCCGCCGCGACCGGCGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAAc                                                                                                                             >  1:1590362/1‑186 (MQ=255)
                                                                                                                                             ccaccaGACAATTCGCCGCAAAACCCCAGCCGAGGACAAAGCCCAAAAACCCCAGCGTCCCCGGTTTTACCACCGGATTGCCCCTCCAGAATGCCAGCGCACCGCCCATCGACCCCTGTTGGCGCCCCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACa                                                                                                                            <  2:1587539/186‑1 (MQ=255)
                                                                                                                                                acaggacaaTCCCCACAAACCCCCAGCCGAGGACAACCCCCAAAAACCCCCGCCTCCCCGGTTTAAACCCCGGATTGCCCATCCAGAATGCCCGCCCCCCCCCCATCGACCCCTTTTGCCACCCCATCCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTAc                                                                               <  2:504076/221‑1 (MQ=255)
                                                                                                                                                           gccgcAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGcaacacaca                                                                     >  1:450972/1‑223 (MQ=255)
                                                                                                                                                                aaacCCCCAGCCGAGGACAAAGCCCATAACCCCCAGCGCCGCCGGTTTAACCCCGGGATTCCCCATCCAAAACGCCAGCGCCCCCCCCACCGACCCCTGTGGGCGACGCATCCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCAcg                                                               <  2:919906/228‑1 (MQ=255)
                                                                                                                                                                 aacccccAGCAGAGACAAAAGCCCAAAACCACCAGCCTCCCCGGGTTTAACCCCGGTTTGCCACTCCAGACTGCCGGCGCCCCCCCCACCGACCCCTGTTGGCGACGCATGCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCAcgc                                                              <  2:1791202/225‑1 (MQ=255)
                                                                                                                                                                                    aGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAACGCGCGATTGCCCAAGCGTACGCAACAACCCGTCACGCGGGATCAGCACCTGAATCa                                           >  1:469748/1‑228 (MQ=255)
                                                                                                                                                                                    aGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCAcgc                                                              >  1:51282/1‑209 (MQ=255)
                                                                                                                                                                                    aGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCa                                           >  1:1551629/1‑228 (MQ=255)
                                                                                                                                                                                    aGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCa                                           >  1:83857/1‑228 (MQ=255)
                                                                                                                                                                                    aGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAACCGTACGCAACAACCAGCCACGCGGGGTCAGCACCTGAATCa                                           >  2:1238375/1‑228 (MQ=255)
                                                                                                                                                                                     gCCCAAAAACCCCGGCGTCCCGGTGTTTACCCCCGGCGTCCCCACCCAGAACGCCAGCCCCCCCCCAACCGACACCGGTGGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCaa                                          <  2:552854/228‑1 (MQ=255)
                                                                                                                                                                                      cccATAAACACCAGCGTCGCCGGTTTTAACCCCGGATTCCCCATCCAGAATGCCAGCGCACCGCCCATCGACCCCTTTGGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCaa                                          <  1:529532/227‑1 (MQ=255)
                                                                                                                                                                                         aTAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGa                                       >  1:1111344/1‑227 (MQ=255)
                                                                                                                                                                                         aTAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGAACGCACCAACCAGTCACGCGGGATCAGCACCTGAATCACCGa                                       >  1:925967/1‑227 (MQ=255)
                                                                                                                                                                                                ccAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAgg                                >  1:2038139/1‑227 (MQ=255)
                                                                                                                                                                                                 cAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGa                               >  1:1154571/1‑227 (MQ=255)
                                                                                                                                                                                                  gccgccGCCGGTTTAAACCGCGTATTCCCCACCCAGAACCCCCGCGCCCCGCCCCCCGCCACCGGTGCGCGCCCCACTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCg                                   <  2:300862/217‑1 (MQ=255)
                                                                                                                                                                                                  agccccGCCAGTTTTACCCCCGGACTCCCCCTCCAGAATGCCACCGCCCCCCCCCTCGCCCCTGGTCGCCCACGCATCCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATc                             <  2:1109352/223‑1 (MQ=255)
                                                                                                                                                                                                   gCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGCACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCCAAAGCCCGATTGCCCAACCGTACGCAACAACCCGTCACCCGGGAACAGCACCTGAATCAACGAACCGCGGAACa                            >  1:1942526/1‑228 (MQ=255)
                                                                                                                                                                                                    cGTCGCCGGGTTTAACCCCGGATTCCCCATCCAGAATGCCAGCGCCCCGCCCATCGACCCCTGTTGGCGCCGCATCCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAAcc                                    <  2:1939082/219‑1 (MQ=255)
                                                                                                                                                                                                    cGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGGTCa                            >  1:59386/1‑227 (MQ=255)
                                                                                                                                                                                                        gCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGaa                                              >  1:1638845/1‑205 (MQ=255)
                                                                                                                                                                                                         ccGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCAcag                                                                                                                                                           >  1:1581357/1‑95 (MQ=255)
                                                                                                                                                                                                          ggggTTTAACCCCGGATTGCCCATCCAGAATGCCAGCGCCCCGCCCATCGACCCCTGTTGGCGACGCATCCCCGCCGCGACCGGAGCCGCAcagc                                                                                                                                                          <  2:1578553/94‑1 (MQ=255)
                                                                                                                                                                                                           gggTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGCAAACAGCGCTCCCTGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTAAGCCACAACCAGTCACGCGGGATCCGCa                                                    >  1:1764686/1‑196 (MQ=255)
                                                                                                                                                                                                             gTTTAACCACCGGTTTGCCAACCCGGAATCCCAGCGCCCCCCCAACCGACCCCTGTGGGCGCCGCATCCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCAc                                                   <  2:1761566/196‑1 (MQ=255)
                                                                                                                                                                                                                         ggATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAg                                                                    >  1:1110502/1‑168 (MQ=255)
                                                                                                                                                                                                                          gATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGca                                                                                                                                                              >  1:593867/1‑77 (MQ=255)
                                                                                                                                                                                                                           aTTCCCCATCCAGAATGCCAGCGCCCCCCCCATCGCCCCCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGcac                                                                                                                                                             <  2:592895/77‑1 (MQ=255)
                                                                                                                                                                                                                             tGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCAGCACCGCCGCTTTCCATAc   >  1:1255462/1‑228 (MQ=255)
                                                                                                                                                                                                                               cccATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAGCGAACCGAGGATCACCCCCAGCACCGCCCCTTTCCCTAcc  >  2:1387666/1‑228 (MQ=255)
                                                                                                                                                                                                                               cccATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCACCGAACCGCGGATCACCCCCAGCACCGCCGCTTTCCATAcc  >  1:1029205/1‑228 (MQ=255)
                                                                                                                                                                                                                               cccATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGCGGATCACCCCCCGCACCGCCGCTTTCCATaac  >  2:1231893/1‑226 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                               
TCCATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGCACAGCAGGTACACATCATGCCCGGCAACGAAAACAGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACC  >  NC_000913/3297463‑3297911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: