Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 353,610 C→G 12.5% A302G (GCG→GGG)  prpE → propionate‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913353,6100CG12.5% 227.2 / 2.1 85A302G (GCG→GGG) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base C (30/43);  new base G (8/4);  total (38/47)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGATCTCTCGTCGCT‑GGAA‑GTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACG  >  NC_000913/353386‑353836
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         
gtggcTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGa                                                                                                                                                                                                                                         <  1:1028198/227‑1 (MQ=255)
   gcTGGAATCCAACGAACCCTCCTGCATTCCTTCCCCTTCCGGCACGACCGGCAAACCTAAAGGCGGGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTa                                                                                                                                                                                                                                      <  2:1533885/227‑1 (MQ=255)
       gAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTa                                                                                                                                                                                                                                      <  2:1824467/223‑1 (MQ=255)
         aTCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGGGTATCGctt                                                                                                                                                                                                                                >  1:1560899/1‑227 (MQ=255)
             aaCGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGGGACTATCGTTTTCGa                                                                                                                                                                                                                            >  1:1176809/1‑227 (MQ=255)
                       cctGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGGGCATCGGTTACGAACGAGTGTCGa                                                                                                                                                                                                                 >  1:337926/1‑228 (MQ=255)
                         tGCATTCTCTACACTGCCGGCACGACCGGCAAACCTAACGGCGTGAAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAc‑c                                                                                                                                                                                                              <  2:2028126/228‑1 (MQ=255)
                             ttccacacccTTCCGGGACGACCGGCAAACCTAAAGGCGTGCAGCGTGGCGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTgg                                                                                                                                                                                                           <  1:849098/218‑1 (MQ=255)
                             ttCTCTACCCTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGGCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTgg                                                                                                                                                                                                           <  1:1418330/227‑1 (MQ=255)
                                tatACATTTCCGGCACGACCGGCCAACCTAAAGGCGTGCTGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGCCCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGcc                                                                                                                                                                                                         <  2:1297303/224‑1 (MQ=255)
                                        tCCGGGACGGCCGGCCAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTgcggc                                                                                                                                                                                               <  1:310776/228‑1 (MQ=255)
                                        tCCGGCACGAACGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTgcggc                                                                                                                                                                                               <  2:1807705/228‑1 (MQ=255)
                                         ccGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGGGACTATGGGTTACGAAGGGTTGCCGAC‑CTGGCCGGACTgcgggg                                                                                                                                                                                              >  1:152539/1‑226 (MQ=255)
                                         ccGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGGATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGGCTCATGTTTTTGAAGGAATGCCGCC‑CTGGGCgggcgtgcgcg                                                                                                                                                                                              >  1:1164217/1‑219 (MQ=255)
                                          cGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGAGTGGGACTCTCGTTTACGAAGGATTGCCGACACTGCCCGG‑CTgcggc‑gg                                                                                                                                                                                            >  1:1826922/1‑227 (MQ=255)
                                          cGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGGGGTGGGGCATTCGGATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGACTCTCGGTTACGAAGGATTGCCGAC‑CTGGCCgggagtcgggt                                                                                                                                                                                              >  1:1559638/1‑218 (MQ=255)
                                           ggCACGACGGGCAAACCTATAGGCGTTCAGCGTGCCGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑gtg                                                                                                                                                                                           <  2:1452807/228‑1 (MQ=255)
                                           ggCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGGCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGa                                                                                                                                                                                                                                         <  1:1965797/184‑1 (MQ=255)
                                            gCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGGGACGATCGGTTACGAAGGAATTCCGAA‑CTGGCCGGGCTgcggc‑gctg                                                                                                                                                                                          >  1:305344/1‑225 (MQ=255)
                                            gCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCg                                                                                                                                                                                                                                   >  1:1021286/1‑189 (MQ=255)
                                             cACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGt                                                                                                                                                                                                                                  <  2:1019426/189‑1 (MQ=255)
                                                 aCCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGGCTATCGTTTACGAAGGATTGCCGAC‑CTGGCCCGACTGCGGC‑GTGt‑ggtgg                                                                                                                                                                                    >  1:1561244/1‑228 (MQ=255)
                                                   cGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTgt‑ggt                                                                                                                                                                                      <  1:906157/224‑1 (MQ=255)
                                                          ccTAAAGGCGTGCAGCGTGACGTCGGCGGATAGGCGGTGGCGCTGGCGCCCTCGATGGACACCATTTTGGGCGGCAAAGCGGGCAGCGTTTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAAtcgt                                                                                                                                                                            <  1:1730130/227‑1 (MQ=255)
                                                              aaGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCgaga                                                                                                                                                                       <  2:1048525/228‑1 (MQ=255)
                                                              aaGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCgag                                                                                                                                                                        <  1:1994258/227‑1 (MQ=255)
                                                                    tGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGGGGTGGGGCATTCGGATATCGTTTACGCGCCGCTGCTGGGGGGGGTGGCGGACAACGGTTACGAAGGAATGGCGAC‑CTGGGCGGGGCGCTGC‑GTGTGGGTGGGC‑ATAGTCGAGCAataat                                                                                                                                                                 >  2:1180844/1‑226 (MQ=255)
                                                                     gCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTgg                                                                                                                                                                                                           <  1:43006/187‑1 (MQ=255)
                                                                      catcGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAg                                                                                                                                                               <  2:1185060/225‑1 (MQ=255)
                                                                                   gcggATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGGGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGAGGGCGGCTATCGTTTACGAAAGATTGCCGAC‑CTGGCCGGACTTCGGC‑GTGT‑GGTGGACAATCGTCGCGAAAAATCAGGTTAGCCggcgg                                                                                                                                                   >  1:1526978/1‑224 (MQ=255)
                                                                                         atGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGGGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGGGACTATCGTTTACGAAGGATGTCCGAC‑CTGGCCGGGCTGCGGC‑GTGT‑GGGGGGCGATCGTCGAGAAAAATCAGGGTAGCCggtggtttgac                                                                                                                                             >  2:144183/1‑218 (MQ=255)
                                                                                               tGGCGCTGGCGACCTCGATGGACCCCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCg                                                                                                                                                                                                        <  1:716147/164‑1 (MQ=255)
                                                                                                              ctgtGGACCCCTTTTTTGGCTGCGAAGGGGGCGGCGTGTTCTTTGCCGCATGGGATATCGGCTGGGGGGTGGGTCGTTCGATTAGGGTTGCCGCGCCGCAGCGGGCGGGGAGGGCGACTATCGTTTACGAAGGATTGCCGCC‑ATGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATTTTCTCAGCGCCGACCGCCATTCGCGTg                                                                                                                        <  2:1607553/224‑1 (MQ=255)
                                                                                                                            tgtGGCGGCAAAGCGGGCAGAGTGTTCTTTTGCGCATCGGAGAGCGGCTGGGTGGTGGGGCATTCGTATATCGTTTCCGCGCCGCGGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTccc                                                                                                         <  2:304823/225‑1 (MQ=255)
                                                                                                                               ggcggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACt                                                                                                                                                                                                                                       >  1:1573347/1‑102 (MQ=255)
                                                                                                                                gcggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTa                                                                                                                                                                                                                                      <  2:1570556/102‑1 (MQ=255)
                                                                                                                                 cggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAAtcgtcg                                                                                                                                                                          <  1:43660/158‑1 (MQ=255)
                                                                                                                                  ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCg                                                                                                                                                                                                                                   >  1:338266/1‑103 (MQ=255)
                                                                                                                                  ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTAc                                                                                                                                                                                                                              >  1:35361/1‑108 (MQ=255)
                                                                                                                                   gcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGt                                                                                                                                                                                                                                  <  2:337696/103‑1 (MQ=255)
                                                                                                                                   gcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACg                                                                                                                                                                                                                             <  2:35295/108‑1 (MQ=255)
                                                                                                                                             ctgCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCa                                                                                       <  1:345749/225‑1 (MQ=255)
                                                                                                                                                      cTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTATCGTTTTCGAAGGATTGCCGAC‑CTGGCCGGGCTGCGGC‑GGGT‑GGTGGGCAATCGTCGGGAAATATCAGGTTAGCCCGATGTTTTCAGCGCCGACCCCCCTTACAGTGGTGGAAAAAATTCCTTA‑CG‑CTGAAATTTGCAAAAACGAtac                                                                             >  2:1991906/1‑226 (MQ=255)
                                                                                                                                                          tGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGGGGCGACTTTCGTTTTCGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GGGT‑GGGGGGCAATCGGCGAGAAATATCAGGGTAGCCGGATGTTTTCAGCGCCGACCGCAATTTGCGGGCT‑GGTAAAATTCCCTACCG‑CTGTAAATCGCCAACACCAtcccccg                                                                         >  2:1688086/1‑223 (MQ=255)
                                                                                                                                                            cgcATCGGATATCGGCTGGGGGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGGCTATCGTTTACGAAGGATTGCCGAC‑CTGGCCgg                                                                                                                                                                                                       >  2:1427583/1‑104 (MQ=255)
                                                                                                                                                             gcATCGGGTGTGGGCTGGGTGGTGGGGCGTTCGTATATCGTTTGAGCGGCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCGGGACTGCGGC‑GTGT‑TGTGGACAATCGTCGAGAAATATCAGGTTAGc                                                                                                                                                         <  1:991974/147‑1 (MQ=255)
                                                                                                                                                             gcATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGa                                                                                                                                                                                                      <  1:1430140/104‑1 (MQ=255)
                                                                                                                                                                 cGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATGGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGATCTCGCGTCGCT‑gg                                                                 <  1:938102/228‑1 (MQ=255)
                                                                                                                                                                   gttttCGGCTGGGTGGTGGGCCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGATCTCTCGTCGCT‑GGaa                                                               <  2:1823685/224‑1 (MQ=255)
                                                                                                                                                                   gaattCGGCTGGTTGGTGGGGCATTCGTATATCGGTTCCGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGATCTCTCGTCGCT‑GGaa                                                               <  1:1318892/224‑1 (MQ=255)
                                                                                                                                                                      atggcgtGGGTGGTGGGGCATGCGTATTTCGTTTCCCCGCCCATGCGGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGATCTCTCGTCGCT‑GGAA‑GTg                                                           <  1:1961146/222‑1 (MQ=255)
                                                                                                                                                                      atCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGGGGGGACTATCGGTTTCGAAGGATTGCCGCC‑CCGGCCGGACTGCGGCGGGGG‑GGGGGACAA‑CGTCGGGGAAAATCAGGGTAGCCCGGGGGTTTAGGGGCCGACCGCCATTGCCGGGCT‑GGAAAAATTCCCAACCC‑CGTGAATTCGCAAAAACGAGTGCTCCTCGCTCGGAA‑gg                                                            >  2:1777974/1‑227 (MQ=255)
                                                                                                                                                                          gCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGATCTCTCGTCGCT‑GGAA‑GTGctc                                                        <  1:384547/227‑1 (MQ=255)
                                                                                                                                                                            tGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGGGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGATCTCTCGTCGCT‑GGAA‑GTGCTCTAt                                                     <  2:1174702/228‑1 (MQ=255)
                                                                                                                                                                             gggTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTTCGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCCTTTGCGTGCT‑GAAAAAATTCCCTACCT‑CTGAAAATCGCCAACACGATCTCTCGTCTCT‑GGAA‑GAGCTCTAt                                                     >  2:199090/1‑227 (MQ=255)
                                                                                                                                                                              ggtggtGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑gt                                                                                                                                                                                            >  1:394365/1‑96 (MQ=255)
                                                                                                                                                                              gggggtggGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CCGGCCGGGCTGCGGC‑GGGG‑GGGGGGCAAACGGCGGGGAAAATCAGGGTTGGCGGGTGGTTTCCGGCCCGGCCCCCCATCCCGTGCT‑GGAAAAAATCTCTACCGCCGGAAATTCCCACACCGCAACCCTCGGCCCT‑GGAA‑GGGCCCTac                                                     >  2:1961212/4‑226 (MQ=255)
                                                                                                                                                                               gtggtgGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑gtg                                                                                                                                                                                           <  2:393716/96‑1 (MQ=255)
                                                                                                                                                                                       gCATTCGTAGATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGATCTCTCGTCGCT‑GGAA‑GTGCTCTATCTGGCTGgag                                           <  1:1995455/227‑1 (MQ=255)
                                                                                                                                                                                        cATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCCTTTCCGTGCT‑GAAAAAATTACCTACCG‑CTGAAATTCGCAAACACGAACTCTCGTCGCT‑GGac                                                               >  2:992278/1‑206 (MQ=255)
                                                                                                                                                                                        cATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGc                                                                                        >  1:38430/1‑183 (MQ=255)
                                                                                                                                                                                         aTTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCa                                                                                       <  2:38360/183‑1 (MQ=255)
                                                                                                                                                                                         aTTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTGACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGATCTCTCGTCGCT‑GGaa                                                               <  1:994066/206‑1 (MQ=255)
                                                                                                                                                                                          ttCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTTCCTACCG‑CTGAAATTACCGAACACGAGCTTCCGTCGCT‑GGAA‑GTGgtcccgctccttc                                              >  2:2017899/1‑211 (MQ=255)
                                                                                                                                                                                          ttCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGGCTATCGTTTACGAAGGATTGCCGAC‑CCGGCCGGGCGTCGGC‑GGGG‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGGTGTTCTCAGCGCCGACCGCCATTCGCGGGCT‑GAAAAAATTTCCTACCG‑CTGAAATTCGCAAACACGAACTCTCGTCGCT‑GGGA‑CTGATCTATc                                                    >  2:449122/1‑215 (MQ=255)
                                                                                                                                                                                           tcgttgatCTTTTACGGGCCGCTGCTGGCGGGGATGGGGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCAAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGATCTCTCGTCGCT‑GGAA‑GTGCTCTATct                                                   <  1:449857/209‑1 (MQ=255)
                                                                                                                                                                                           tCGTATATCGGTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGATCTCTCGTCGc                                                                     <  1:2021508/199‑1 (MQ=255)
                                                                                                                                                                                                  tCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CCGAAATTCGCAAACACGAACTCTCGTAGCT‑GGAA‑GTGCTCCATCTGGCCGGGGaaagcctggagt                               >  2:2025790/1‑218 (MQ=255)
                                                                                                                                                                                                     tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGa                                                                                                                                                     >  1:429469/1‑111 (MQ=255)
                                                                                                                                                                                                     tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGG‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGACCTCTCGTCGCT‑GGAA‑GGGCTCTATCCGGCCGGTGAAACGCTGGACGAgg                            >  2:864054/1‑227 (MQ=255)
                                                                                                                                                                                                     tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GGGT‑GGGGGGCAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTTGCCAAAACGAACTTCTGTCCCT‑GGCA‑GTGCTCTATCTGGCTGGAGGACCGCTGGACGAGc                            >  2:1354928/1‑228 (MQ=255)
                                                                                                                                                                                                     tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CCGGCCGGGCTGCGGC‑GTGT‑GGGGGACAATCGTCGAGAAATATCAGGTTAGCCGGGTGGTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTTCCTACCG‑CTGAAATTCGCAAACACGATCTCTCGTCCCT‑GGAA‑AGGCCCTAACTGGCTGGGGAACCGCTGGACGAGc                            >  2:713135/1‑228 (MQ=255)
                                                                                                                                                                                                     tttACGCGCCGCTGCTGGCGGGGATGGCGACCATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTggggc‑gg                                                                                                                                                                                            >  2:1455317/1‑72 (MQ=255)
                                                                                                                                                                                                      ttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑gtg                                                                                                                                                                                           <  1:1457921/73‑1 (MQ=255)
                                                                                                                                                                                                      ttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGAt                                                                                                                                                    <  2:428753/111‑1 (MQ=255)
                                                                                                                                                                                                       tACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTTCCGAC‑CTGGCCGGACTGCGGC‑GGGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CCGTAATTCGCAAAAACGATCTCTCGGCGCT‑GGAA‑GGGGTCCATCTGGCTTGGGAACCCCTGGACGAGcca                          >  2:1357587/1‑227 (MQ=255)
                                                                                                                                                                                                       tACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCCTTTGCGTGCT‑GAAAAAATTTCCTACCG‑CTGAAATTTGCAAAAACGATCTCCCCTCGCT‑TGAAGGTGC‑CTATCTTGCTGGGGGACCTCCGGGCGatgcc                          >  2:1136793/1‑224 (MQ=255)
                                                                                                                                                                                                               gctgctGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGATCTCTCGTCGCT‑GGAA‑GTGc                                                          >  1:58737/1‑188 (MQ=255)
                                                                                                                                                                                                               gctgctGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGG‑GGGGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGCCCGCCATTCCCGTGCT‑GAAAAAATTCCCTACCG‑CTGCAATTTGCCAACACGAACTCCCGGCGCC‑GGAA‑GTGGCCTATCTTGCTGGGGACACGCCGGCAGAGCGGAGCGcccg                  >  2:1425311/1‑226 (MQ=255)
                                                                                                                                                                                                                ctgcggGGGGGAATGGCGCTTTTCGTTTACGAAGGATTCCCGCC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGATCTCTCGTCGCT‑GGAA‑GTGct                                                         <  2:58631/188‑1 (MQ=255)
                                                                                                                                                                                                                   cgggggggaATGGCGGCTATCGTTTACGAAGGATTCCCGAC‑CTGGCCGGACTGGGGC‑GTGT‑GGTGGACAATCGGCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGATCTCTCGTCGCT‑GGAA‑GTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTgg              <  1:1360030/223‑1 (MQ=255)
                                                                                                                                                                                                                     gggggggATGGGGACTATAGTATACGGAGGTGTGCCGCC‑CTGGCCGGACTGCGGG‑GTGT‑GGTGGACAATCGTCGAAAAATATGAGGTTAGCCGGATTTTCCCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGATCTCTCGCCGCT‑GGAA‑GTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGt            <  2:337357/225‑1 (MQ=255)
                                                                                                                                                                                                                        ggggATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGG‑GGGGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAgcgc                                                                                                                                         >  2:1890471/1‑104 (MQ=255)
                                                                                                                                                                                                                         gggATGGGGACTTTCGTTTACGAAAGATTGCCGAC‑CTGGCCGGCCTGGGGC‑GTTT‑GGTGGACAATCGTCGAGAAATATCAGTTTAGCCGGTTGTTCTCAGCGcc                                                                                                                                        <  1:1893819/104‑1 (MQ=255)
                                                                                                                                                                                                                          gaaTGGCGACTACCGTTTACGAAGGATGGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGATCTCTCGTCGCT‑GGAA‑GTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCa       <  1:1357367/226‑1 (MQ=255)
                                                                                                                                                                                                                                cGACTATCGTTTACGAAGGTTTGCCGAC‑CTGGCCGGACGGCGGC‑GTGT‑GGTGGACAATCGGCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGATCTCTCGTCGCT‑GGAA‑GTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACg  <  1:509388/227‑1 (MQ=255)
                                                                                                                                                                                                                                cGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGATCTCTCGTCGCT‑GGa                                                                >  2:46245/1‑166 (MQ=255)
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         
GTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGAC‑CTGGCCGGACTGCGGC‑GTGT‑GGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCT‑GAAAAAATTCCCTACCG‑CTGAAATTCGCAAACACGATCTCTCGTCGCT‑GGAA‑GTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACG  >  NC_000913/353386‑353836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑