| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 514,757 | T→C | 15.7% | S35G (AGC→GGC) | ybbJ ← | inner membrane protein; stimulator of the QmcA suppressor of ftsH‑htpX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 514,757 | 0 | T | C | 15.7% | 95.4 / 2.1 | 36 | S35G (AGC→GGC) | ybbJ | inner membrane protein; stimulator of the QmcA suppressor of ftsH‑htpX |
| Reads supporting (aligned to +/- strand): ref base T (27/3); new base C (2/4); total (29/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.61e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
AAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGA > NC_000913/514557‑514977 | aaaaCGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCAACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAACCACCGCTGCCACGCCGACCCACAACAAACAACCATTTCCGCCCa > 1:1435455/1‑227 (MQ=255) gcCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGCAATCACCGCTGCCCCGCCGCTCCACAACCAACAACCATTTCCGCCCAGCATCTCGCATGCCcgcagc > 1:1619944/1‑224 (MQ=255) cTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCACAACAACTAACCATTTCCGCCCAGCATCTCGGCCGCCACCAACAAACCGCCGCGACTGAGCCAGaaa > 1:453458/1‑228 (MQ=255) cTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCACAACAACTAACCATTTCCGCCCAGCATCCCGGCTGCCAGCAGCCAACCGCCCAGACCGATCCa > 1:1774775/1‑224 (MQ=255) cTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGaaa > 1:1046801/1‑228 (MQ=255) tgCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCCGAAAATATGGGGa > 1:1048244/1‑228 (MQ=255) tgCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGa > 1:1547181/1‑228 (MQ=255) ttGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGGTGaa > 1:1182232/1‑227 (MQ=255) cgcACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCCCCACAACAAATAACCATTTCCGc > 1:1295599/1‑160 (MQ=255) caccacAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAAt > 1:1940862/1‑124 (MQ=255) caccacAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTc > 1:1202226/1‑224 (MQ=255) accacAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATa < 2:1937421/124‑1 (MQ=255) cgAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCCGAAAATATGTGGATGAACGACCATTAACCCCATCCTGGCTGAGTCCGTTTGTTGGCGCTGTCtt > 1:231970/1‑227 (MQ=255) agcagcGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCacaaca > 2:409907/1‑103 (MQ=255) gcagcGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCAcaacaa < 1:410589/103‑1 (MQ=255) gcgcGTCAGGAAGGCAAACAATACCCCTTGCCCCCCCCAACCCGGCCG‑GCCAGCGCCAACCACCC‑GCGCCGGAAACCCCCGCTCCCCCGCCGACCCAAAACAAAGACCCATTCCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCAc < 2:925388/226‑1 (MQ=255) aTGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGGCCGTTTGTTGGTGCTGTCTTTTACCAGCTCGGCaa > 2:1045011/1‑228 (MQ=255) ccccTTGCCACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGgcg > 1:56209/1‑193 (MQ=255) tGCCACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGa > 1:106453/1‑147 (MQ=255) cccccccccACCCCAGCGCCCCCCCC‑CAACCCCCCA‑G‑CCCGGTAACCCCCGCTCCCACGCCGCCCCACAACAAATAACCATTCCCCCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGaa < 2:106263/141‑1 (MQ=255) tCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGAc > 1:196379/1‑218 (MQ=255) cccAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTgg > 2:1128373/1‑228 (MQ=255) ggcgcCACCACC‑AAGCCCCACA‑G‑GCAGGTACCCACCGCTCCCCCGCCGCCCCAAAACAAACAACCATGCCCCCCAAGCACCCCGGCGGCAAGCAGCAACCCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCct < 2:1086697/227‑1 (MQ=255) aGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGCCTGCTGGcgt > 1:289455/1‑226 (MQ=255) gCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGcc > 1:1088644/1‑226 (MQ=255) cgaCCAGC‑CCTACCCCCAGG‑GCCGATACCCCCCGCGGCCAGCCCGTCCCAAAAAAAATACCCATTCCCCCCCAGACTCCGGGCTGCCAGCAACAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTa < 2:231576/226‑1 (MQ=255) cGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCt > 2:735565/1‑228 (MQ=255) cGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACACCATCAGGCTGCTGGCCTCTCATGGCCTCAGCACTACCTTGCt > 2:1876797/1‑228 (MQ=255) ccGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCa > 2:1951744/1‑152 (MQ=255) cGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAg < 1:1955226/152‑1 (MQ=255) cTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTgga > 2:1564799/1‑225 (MQ=255) tGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGGCTCTAATGGCATCATCACTACTTTGCTGTTACTgga > 2:1334420/1‑224 (MQ=255) tGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGC‑GTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTggt > 2:682229/1‑222 (MQ=255) aCGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGa > 2:793846/1‑228 (MQ=255) cTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATcc > 1:921234/1‑150 (MQ=255) cTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGgctgc > 1:1513699/1‑174 (MQ=255) tCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATccc < 2:919617/150‑1 (MQ=255) tCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGgctgct < 2:1510990/174‑1 (MQ=255) | AAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGC‑CAGACCACCA‑G‑GCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCATTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGGCAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGA > NC_000913/514557‑514977 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |