Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913986,5510AC24.1% 517.0 / 200.6 276T144T (ACT→ACG) ompFouter membrane porin 1a (Ia;b;F)
Reads supporting (aligned to +/- strand):  ref base A (104/104);  new base C (27/39);  total (133/143)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.71e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 277 total aligned reads displayed.

ACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTAT  >  NC_000913/986453‑986650
                                                                                                  |                                                                                                   
aCAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAg                                                                                                    <  2:2389038/100‑1 (MQ=255)
 cAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGt                                                                                                   <  2:3651006/100‑1 (MQ=255)
 cAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGt                                                                                                   <  1:1027546/100‑1 (MQ=255)
 cAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGCAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGt                                                                                                   <  2:3152225/100‑1 (MQ=255)
 cAGCGAAGTTCAGGCCAGCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCAGCGCTGTATGCAGt                                                                                                   <  2:1481206/100‑1 (MQ=255)
  aGCGGAGATCAGGCCATCAACCAGACCTAAGAAGCTTGAGGTACGATAGGTATCAACGCCGCCAATACGACCATTGAAGAAGTCATCGCTGTATGCCGtg                                                                                                  <  2:4140053/100‑2 (MQ=255)
  aGCGACGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGATGTCATCGCTGTATGCAGTa                                                                                                  >  2:1538671/1‑100 (MQ=255)
  aGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGtg                                                                                                  <  2:1176121/100‑2 (MQ=255)
   gCGAAGTTCAGGCGATGAACCAGGCCGAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACTAACGAAGAAGTCATCTCTGCACGCAGTAt                                                                                                 <  2:3579370/100‑1 (MQ=255)
   gCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTAt                                                                                                 >  1:1463834/1‑100 (MQ=255)
    cGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATc                                                                                                >  2:2628204/1‑100 (MQ=255)
     gAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATca                                                                                               <  2:845144/100‑1 (MQ=255)
      aaGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGGCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATcac                                                                                              <  1:3752105/100‑1 (MQ=255)
       aGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATcacc                                                                                             <  2:2272653/100‑1 (MQ=255)
       aGTTCAGGCCAACAACCAGCCCAAAGAACTTGGAGTTACGATAGGTAGCAACGCCGCCATCACGACCATCGAAGAAGTCATCGCTGTATGCCGTGTcacc                                                                                             <  2:2801950/100‑1 (MQ=255)
        gTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTcaccg                                                                                            <  2:1099173/100‑2 (MQ=255)
        gTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTcaccg                                                                                            <  2:3931332/100‑2 (MQ=255)
        gTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATcacca                                                                                            <  1:483599/100‑1 (MQ=255)
        gTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATcacca                                                                                            <  2:2223535/100‑1 (MQ=255)
         ttCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATcaccac                                                                                           <  2:170196/100‑1 (MQ=255)
          tCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCaccgcc                                                                                          <  2:381147/100‑1 (MQ=39)
           cAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCAccacca                                                                                         >  1:2566574/1‑100 (MQ=255)
            aGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCaa                                                                                        >  1:3130549/1‑100 (MQ=255)
            aGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCaa                                                                                        >  2:1337499/1‑100 (MQ=255)
              gCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCGACACGACCAACGCAGAGGTCATCGCTGTATGCAGTATCACGACCAAAt                                                                                      >  1:1540878/1‑100 (MQ=38)
              gCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAAt                                                                                      <  2:3761792/100‑1 (MQ=255)
              gCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAAt                                                                                      <  2:295016/100‑1 (MQ=255)
               ccATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAAtt                                                                                     <  2:3078372/100‑1 (MQ=255)
                 aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGCAGAAGTCATCGCTGTATTCAGTATCACCACCAAATTCt                                                                                   >  2:497762/1‑100 (MQ=255)
                 aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCt                                                                                   <  1:734283/100‑1 (MQ=255)
                    aaCCATACCAAGGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGGCATCGCTGTATGCAGTATCACCACCAAATTCTGgc                                                                                <  2:396523/100‑1 (MQ=255)
                    aaCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgc                                                                                >  2:2566546/1‑100 (MQ=255)
                    aaCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgc                                                                                <  2:894054/100‑1 (MQ=255)
                    aaCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgc                                                                                <  1:1567863/100‑1 (MQ=255)
                    aaCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgc                                                                                <  1:1784521/100‑1 (MQ=255)
                     aCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgca                                                                               <  1:91356/100‑1 (MQ=255)
                      ccAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgcag                                                                              <  2:1966777/100‑1 (MQ=255)
                      ccAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgcag                                                                              >  2:3384530/1‑100 (MQ=255)
                         gACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCat                                                                           >  1:2775652/1‑100 (MQ=255)
                            cAACGAAGTTGGCGCTACCATAGGTAGCAACGCCGCCAACCCGACCAACGAACAAGTCATCACTCTATGCGGTAACACCCCCAAATTCTGGCAGCatcaa                                                                        >  2:4088883/1‑97 (MQ=255)
                            cAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc                                                                        <  2:2041740/100‑1 (MQ=255)
                             aaaGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCg                                                                       <  1:2067943/100‑1 (MQ=255)
                              aagaagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCgg                                                                      <  2:3608375/100‑1 (MQ=255)
                              aagaagTGGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCgg                                                                      <  2:3042634/100‑1 (MQ=255)
                                gaagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATGGgtg                                                                    <  2:3688966/100‑1 (MQ=255)
                                   gTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTaa                                                                 >  1:2485015/1‑100 (MQ=255)
                                   gTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTaa                                                                 >  2:3101815/1‑100 (MQ=255)
                                    ttGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAAc                                                                <  2:2346220/100‑1 (MQ=38)
                                      ggAGTTACGATAGGTAGCAACGCCGCCAACACGACCAAGGAAGAAGGCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATAGCGGTGTAAccc                                                              >  1:114452/1‑100 (MQ=255)
                                      ggAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAAccc                                                              <  1:2235476/100‑1 (MQ=255)
                                        aGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAg                                                            <  1:2049210/100‑1 (MQ=255)
                                         gTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGt                                                           >  1:3728301/1‑100 (MQ=38)
                                         gTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGt                                                           >  2:4318845/1‑100 (MQ=255)
                                          ttACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTg                                                          <  2:777262/100‑1 (MQ=255)
                                            aCGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCGGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGca                                                        >  2:2153090/1‑100 (MQ=255)
                                             cGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGcat                                                       >  1:2998672/1‑100 (MQ=255)
                                              gATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGcatc                                                      <  2:788292/100‑1 (MQ=38)
                                               aTAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGcatca                                                     >  1:2026366/1‑100 (MQ=38)
                                                  ggTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATaa                                                  <  2:208209/100‑1 (MQ=38)
                                                   gTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATaaa                                                 >  2:2535472/1‑100 (MQ=255)
                                                     aGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAAcc                                               <  2:3772439/100‑1 (MQ=38)
                                                     aGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAAcc                                               >  1:639226/1‑100 (MQ=255)
                                                       cAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACcac                                             <  2:1207716/100‑1 (MQ=255)
                                                          cgccgcCAACTCGACCAACGAAGAAGTCATCGCTGTATGCCGTGCCACCGCCAAACTCAGGCAGCCTATCGGTGTAACCCAGTGCATCATAAACCCCAcc                                          >  1:3865817/1‑100 (MQ=255)
                                                          cgccgcCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACAcc                                          >  1:833185/1‑100 (MQ=38)
                                                           gccgccAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCg                                         >  1:1640464/1‑100 (MQ=38)
                                                           cccgccAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCg                                         >  1:1704136/2‑100 (MQ=38)
                                                              gccAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAg                                      <  2:254356/100‑1 (MQ=255)
                                                                cAACACGACCAACGAAGAAGTCATCGCTGTACGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGtt                                    >  2:1804570/1‑100 (MQ=255)
                                                                 aaCACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTa                                   <  1:1957861/100‑1 (MQ=255)
                                                                   cacGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACg                                 <  1:2603016/100‑1 (MQ=255)
                                                                     cGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGc                               <  2:2020636/100‑1 (MQ=255)
                                                                     cGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGc                               >  2:1739537/1‑100 (MQ=255)
                                                                       aCCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCg                             >  1:3276252/1‑100 (MQ=255)
                                                                        ccAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGt                            >  1:3043974/1‑100 (MQ=255)
                                                                        ccAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGt                            <  2:602512/100‑1 (MQ=255)
                                                                         cAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTa                           >  2:1664152/1‑100 (MQ=255)
                                                                           aCGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAAt                         <  2:1444087/100‑1 (MQ=255)
                                                                              nagaagTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGa                      >  1:3518476/2‑100 (MQ=255)
                                                                               agaagTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGaa                     <  2:1380466/100‑1 (MQ=255)
                                                                                gaagTCATCGCTGGATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTATTTACGGCCGTATTCGaaa                    <  1:3675386/100‑1 (MQ=255)
                                                                                 aagTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAg                   <  1:4250187/100‑1 (MQ=255)
                                                                                    tCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAAc                <  2:3231447/100‑1 (MQ=255)
                                                                                    gcATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAAc                >  1:3930342/2‑100 (MQ=38)
                                                                                     cATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAAcc               >  1:4407990/1‑100 (MQ=38)
                                                                                     cATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAAcc               >  1:1884716/1‑100 (MQ=255)
                                                                                     cATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAAcc               >  1:1309452/1‑100 (MQ=255)
                                                                                      aTCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCa              >  1:1845839/1‑100 (MQ=255)
                                                                                         gCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACg           <  1:2412869/100‑1 (MQ=255)
                                                                                         gCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACg           <  2:2711496/100‑1 (MQ=255)
                                                                                         gCTGTATGCAGTATCACCACCAAATTCTGGCAGCATACCGGTGTAACCCAGTGCATCATAAACCACACCCCAGTTACGGCCGTAATCGAAAGAACCAAcc           >  2:4292884/1‑99 (MQ=255)
                                                                                             tATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAg       <  2:3841071/100‑1 (MQ=255)
                                                                                              aTGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGc      >  1:68330/1‑100 (MQ=255)
                                                                                               tGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCg     >  1:3437127/1‑100 (MQ=38)
                                                                                               tGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCg     >  2:250258/1‑100 (MQ=255)
                                                                                                gCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGt    >  1:435770/1‑100 (MQ=255)
                                                                                                 cAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTa   <  2:4208432/100‑1 (MQ=255)
                                                                                                 cAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTa   >  1:3229656/1‑100 (MQ=255)
                                                                                                 cAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACCTCAGCGTa   >  1:3642429/1‑100 (MQ=255)
                                                                                                  aGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTAt  <  2:813668/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
ACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTAT  >  NC_000913/986453‑986650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑