Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913986,5540AG22.6% 511.7 / 149.1 265D143D (GAT→GAC) ompFouter membrane porin 1a (Ia;b;F)
Reads supporting (aligned to +/- strand):  ref base A (103/102);  new base G (26/34);  total (129/136)
Fisher's exact test for biased strand distribution p-value = 3.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 271 total aligned reads displayed.

AGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTA  >  NC_000913/986455‑986653
                                                                                                   |                                                                                                   
 gCGAAGTTCAGGCGATGAACCAGGCCGAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACTAACGAAGAAGTCATCTCTGCACGCAGTAt                                                                                                    <  2:3579370/100‑1 (MQ=255)
   gAAGTTCAGGCCATCAACAAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATca                                                                                                  >  2:3042654/1‑100 (MQ=255)
    aaGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGGCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATcac                                                                                                 <  1:3752105/100‑1 (MQ=255)
     aGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATcacc                                                                                                <  2:17648/100‑1 (MQ=255)
     aGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATcacc                                                                                                <  2:2272653/100‑1 (MQ=255)
     aGTGCAGGCCATCAACCAGACAAAACAAGTTGGAGTCACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATcacc                                                                                                <  2:4110514/100‑1 (MQ=255)
      gTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTcaccg                                                                                               <  2:3931332/100‑2 (MQ=255)
      gTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATcacca                                                                                               <  1:483599/100‑1 (MQ=255)
       ttCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATcaccac                                                                                              <  2:170196/100‑1 (MQ=255)
        tCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCaccgcc                                                                                             <  2:381147/100‑1 (MQ=39)
        tCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCaccacc                                                                                             >  2:676821/1‑100 (MQ=255)
        gcAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCaccacc                                                                                             <  2:4160211/99‑1 (MQ=255)
         cAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCAccacca                                                                                            >  1:2566574/1‑100 (MQ=255)
          gggCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCaa                                                                                           >  1:3711950/2‑100 (MQ=38)
          aGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCaa                                                                                           >  2:1337499/1‑100 (MQ=255)
           ggCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCaaa                                                                                          >  1:1624223/1‑100 (MQ=39)
            gCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCGACACGACCAACGCAGAGGTCATCGCTGTATGCAGTATCACGACCAAAt                                                                                         >  1:1540878/1‑100 (MQ=38)
             ccATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCaaact                                                                                        >  1:2842010/1‑98 (MQ=38)
             ccATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAAtt                                                                                        <  2:1996965/100‑1 (MQ=255)
              cATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTc                                                                                       >  2:2207286/1‑100 (MQ=255)
               aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCt                                                                                      <  2:252072/100‑1 (MQ=255)
               aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGCATCACCACCAAATTCt                                                                                      >  2:3756895/1‑100 (MQ=255)
               aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTAGGCAGTATCACCACCAAATTCt                                                                                      >  1:4115248/1‑100 (MQ=255)
                tCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTg                                                                                     <  1:1249750/100‑1 (MQ=255)
                  aaCCATACCAAGGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGGCATCGCTGTATGCAGTATCACCACCAAATTCTGgc                                                                                   <  2:396523/100‑1 (MQ=255)
                  aaCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGgc                                                                                   >  1:803484/1‑100 (MQ=255)
                  aaCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgc                                                                                   >  2:2566546/1‑100 (MQ=255)
                  aaCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgc                                                                                   >  2:3770511/1‑100 (MQ=255)
                  aaCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgc                                                                                   <  2:894054/100‑1 (MQ=255)
                  aaCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgc                                                                                   <  1:1784521/100‑1 (MQ=255)
                   aCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgca                                                                                  <  1:303450/100‑1 (MQ=255)
                   aCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgca                                                                                  <  1:91356/100‑1 (MQ=255)
                   aCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgca                                                                                  >  1:3353403/1‑100 (MQ=255)
                    ccAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgcag                                                                                 >  2:3384530/1‑100 (MQ=255)
                    ccAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgcag                                                                                 <  2:1966777/100‑1 (MQ=255)
                         ccAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAAGTCTGACAGCatct                                                                            >  2:233502/1‑98 (MQ=255)
                          cAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc                                                                           <  2:2041740/100‑1 (MQ=255)
                           aaaGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCg                                                                          <  1:2067943/100‑1 (MQ=255)
                            aagaagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCgg                                                                         >  2:2647548/1‑100 (MQ=255)
                            aagaagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCgg                                                                         <  2:3608375/100‑1 (MQ=255)
                               aagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGgtgt                                                                      <  1:3458215/100‑1 (MQ=255)
                                 gTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTaa                                                                    >  1:2485015/1‑100 (MQ=255)
                                  ttGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAAc                                                                   <  2:2854778/100‑1 (MQ=255)
                                    ggAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAAccc                                                                 <  1:2235476/100‑1 (MQ=255)
                                      aGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAg                                                               <  1:2049210/100‑1 (MQ=255)
                                       gTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGt                                                              >  1:3178965/1‑100 (MQ=38)
                                       gTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGt                                                              >  1:3728301/1‑100 (MQ=38)
                                       gTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGt                                                              <  2:3007315/100‑1 (MQ=255)
                                            gATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGcatc                                                         <  2:788292/100‑1 (MQ=38)
                                            gATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGcatc                                                         <  2:842009/100‑1 (MQ=255)
                                             aTAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGcatca                                                        >  1:2026366/1‑100 (MQ=38)
                                              tAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCCAACTCCGGCAGCATATCGGTGTAACCCAGTGcatcat                                                       >  1:3587080/1‑100 (MQ=255)
                                                ggTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATaa                                                     >  1:1403699/1‑100 (MQ=255)
                                                ggTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATaa                                                     >  1:1467872/1‑100 (MQ=255)
                                                ggTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATaa                                                     >  2:57430/1‑100 (MQ=255)
                                                  tAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAAc                                                   <  2:3135247/100‑1 (MQ=38)
                                                  tAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAAc                                                   <  2:274227/100‑1 (MQ=38)
                                                  tAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAAc                                                   >  2:79397/1‑100 (MQ=255)
                                                   aGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAAcc                                                  >  1:639226/1‑100 (MQ=255)
                                                   aGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAAcc                                                  <  1:3094973/100‑1 (MQ=255)
                                                    gCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACca                                                 <  2:1638330/100‑1 (MQ=255)
                                                     cAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACcac                                                <  2:1207716/100‑1 (MQ=255)
                                                       aCGCCGCCAACACGCCCATCTAAGAAGGCAGGGCTGTAGGCACTATCACCACCAACCTCCCGCAACATATCGGTTTAACCCAGTGCATCATAAAcccgac                                              <  2:498971/100‑5 (MQ=255)
                                                        cgccgcCAACTCGACCAACGAAGAAGTCATCGCTGTATGCCGTGCCACCGCCAAACTCAGGCAGCCTATCGGTGTAACCCAGTGCATCATAAACCCCAcc                                             >  1:3865817/1‑100 (MQ=255)
                                                        cgccgcCAACACGAGCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATCAACCACAcc                                             <  2:1074699/100‑1 (MQ=255)
                                                        cgccgcCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACAcc                                             >  1:833185/1‑100 (MQ=38)
                                                         gccgccAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCg                                            <  2:1812464/100‑1 (MQ=38)
                                                         cccgccAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCg                                            >  1:1704136/2‑100 (MQ=38)
                                                            gccAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAg                                         <  2:3659966/100‑1 (MQ=38)
                                                            gccAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAg                                         <  2:254356/100‑1 (MQ=255)
                                                             caaaCACAACAAACGAAGAAGTCATCACTGTATGCAGTATAACCAACCAATACTAACAGCAAAACAGTGTAACCCAGTGCATCATAAACCACACCCTata                                        >  2:2617885/3‑98 (MQ=255)
                                                               aaCACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTa                                      <  1:1957861/100‑1 (MQ=255)
                                                                 cacGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACg                                    >  2:1115381/1‑100 (MQ=255)
                                                                 cacGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACg                                    <  1:3646145/100‑1 (MQ=255)
                                                                 cacGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACg                                    <  1:2603016/100‑1 (MQ=255)
                                                                    gACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGcc                                 <  2:2134676/100‑1 (MQ=255)
                                                                     aCCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCg                                <  1:1690529/100‑1 (MQ=255)
                                                                      ncAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGt                               >  1:1028911/2‑100 (MQ=255)
                                                                       cAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTa                              >  2:1664152/1‑100 (MQ=255)
                                                                        aaCGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTaa                             >  1:1895015/1‑100 (MQ=255)
                                                                         aCGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAAt                            <  2:1444087/100‑1 (MQ=255)
                                                                          cGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATc                           <  2:4335257/100‑1 (MQ=255)
                                                                             agaagTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGaa                        <  2:1380466/100‑1 (MQ=255)
                                                                             agaagTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGaa                        >  2:2070584/1‑100 (MQ=255)
                                                                               aagTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAg                      <  2:2386689/100‑1 (MQ=38)
                                                                               aagTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAg                      <  1:2685180/100‑1 (MQ=255)
                                                                                  tCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAAc                   <  2:3231447/100‑1 (MQ=255)
                                                                                  gcATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAAc                   >  1:3930342/2‑100 (MQ=38)
                                                                                   cATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAAcc                  >  1:4407990/1‑100 (MQ=38)
                                                                                    aTCGCTGTGTGCAGTAACACCAGCAAATTCTGGCAGCATACCGGGTTAACCCAGTGCATCATAAACCACACCGTAGTTCCGGCCGGAATGGAAAGAACCa                 >  2:3082761/1‑100 (MQ=255)
                                                                                       gCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACg              <  1:2412869/100‑1 (MQ=255)
                                                                                       gCTGTATGCAGTATCACCACCAAATTCTGGCAGCATACCGGTGTAACCCAGTGCATCATAAACCACACCCCAGTTACGGCCGTAATCGAAAGAACCAAcc              >  2:4292884/1‑99 (MQ=255)
                                                                                           tATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAg          <  2:3841071/100‑1 (MQ=255)
                                                                                            aTGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGc         >  1:68330/1‑100 (MQ=255)
                                                                                            aTGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGc         >  1:323734/1‑100 (MQ=255)
                                                                                             tGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACTCCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCg        <  2:1516338/100‑1 (MQ=255)
                                                                                             tGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCg        >  1:1856749/1‑100 (MQ=255)
                                                                                             tGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCg        >  2:250258/1‑100 (MQ=255)
                                                                                              gCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGt       >  1:435770/1‑100 (MQ=255)
                                                                                                  tATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTAttt   <  2:4210164/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
AGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTA  >  NC_000913/986455‑986653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑