Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913986,5600AG23.3% 438.5 / 142.4 241G141G (GGT→GGC) ompFouter membrane porin 1a (Ia;b;F)
Reads supporting (aligned to +/- strand):  ref base A (96/88);  new base G (28/28);  total (125/116)
Fisher's exact test for biased strand distribution p-value = 8.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.71e-02
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 267 total aligned reads displayed.

GTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCC  >  NC_000913/986461‑986659
                                                                                                   |                                                                                                   
gTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATcacca                                                                                                     >  1:4407259/1‑100 (MQ=255)
gTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATcacca                                                                                                     <  2:118134/100‑1 (MQ=255)
 ttCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATcaccac                                                                                                    <  2:170196/100‑1 (MQ=255)
  tCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCaccacc                                                                                                   <  2:2311660/100‑1 (MQ=255)
  gcAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCaccacc                                                                                                   <  2:4160211/99‑1 (MQ=255)
    aGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCaa                                                                                                 >  1:3130549/1‑100 (MQ=255)
      gCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAAt                                                                                               <  2:780065/100‑1 (MQ=255)
       ccATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAAtt                                                                                              <  2:3078372/100‑1 (MQ=255)
        cATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTc                                                                                             >  2:2207286/1‑100 (MQ=255)
        cATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTc                                                                                             <  2:1379858/100‑1 (MQ=255)
         aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCt                                                                                            <  2:2694457/100‑1 (MQ=255)
         aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCt                                                                                            <  1:1925675/100‑1 (MQ=255)
         aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCt                                                                                            <  1:734283/100‑1 (MQ=255)
         aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTAGGCAGTATCACCACCAAATTCt                                                                                            >  1:4115248/1‑100 (MQ=255)
            aaCCATACCAAGGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGGCATCGCTGTATGCAGTATCACCACCAAATTCTGgc                                                                                         <  2:396523/100‑1 (MQ=255)
            aaCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCTCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgc                                                                                         <  1:2899546/100‑1 (MQ=255)
            aaCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgc                                                                                         >  2:3770511/1‑100 (MQ=255)
            aaCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgc                                                                                         >  1:3908761/1‑100 (MQ=255)
            aaCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgc                                                                                         <  2:894054/100‑1 (MQ=255)
            aaCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgc                                                                                         <  2:83770/100‑1 (MQ=255)
            aaCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgc                                                                                         <  1:1567863/100‑1 (MQ=255)
                aGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACTAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgcagca                                                                                     <  2:351108/100‑1 (MQ=255)
                 gACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCat                                                                                    >  1:2775652/1‑100 (MQ=255)
                 gACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGACATCGCTGTAAGCAGTATCACCACCAAATTCTGGCACCat                                                                                    >  2:1318839/1‑100 (MQ=255)
                   ccAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTATCACCACCAAATTCTGGCAGCatac                                                                                  >  2:3381867/1‑99 (MQ=255)
                    cAACGAAGTTGGCGCTACCATAGGTAGCAACGCCGCCAACCCGACCAACGAACAAGTCATCACTCTATGCGGTAACACCCCCAAATTCTGGCAGCatcaa                                                                                 >  2:4088883/1‑97 (MQ=255)
                    cAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc                                                                                 <  2:2041740/100‑1 (MQ=255)
                     aaaGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCg                                                                                >  2:3732023/1‑100 (MQ=255)
                     aaaGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCg                                                                                <  1:2067943/100‑1 (MQ=255)
                      aagaagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCCGGCAGCATATCgg                                                                               <  2:1788286/100‑1 (MQ=255)
                      aagaagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCgg                                                                               <  2:604220/100‑1 (MQ=255)
                      aagaagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCgg                                                                               <  2:3608375/100‑1 (MQ=255)
                        gaagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGgtg                                                                             >  1:2434845/1‑100 (MQ=255)
                          agTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTa                                                                           >  2:792451/1‑100 (MQ=255)
                           gTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTaa                                                                          >  1:859496/1‑100 (MQ=255)
                           gTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTaa                                                                          >  2:3101815/1‑100 (MQ=255)
                              ggAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAAccc                                                                       >  1:3849396/1‑100 (MQ=38)
                              ggAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAAccc                                                                       >  1:240822/1‑100 (MQ=38)
                              ggAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAAccc                                                                       >  1:3651361/1‑100 (MQ=255)
                              ggAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAAccc                                                                       >  1:2045349/1‑100 (MQ=255)
                              ggAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAAccc                                                                       >  1:1255214/1‑100 (MQ=255)
                              ggAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAAccc                                                                       <  1:2235476/100‑1 (MQ=255)
                               gAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCa                                                                      >  1:3623471/1‑100 (MQ=255)
                                aGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAg                                                                     <  2:2563425/100‑1 (MQ=255)
                                 gTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGt                                                                    >  1:3728301/1‑100 (MQ=38)
                                 gTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGt                                                                    <  2:3007315/100‑1 (MQ=255)
                                 gTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGt                                                                    >  1:258057/1‑100 (MQ=255)
                                  ttACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTg                                                                   <  1:2492369/100‑1 (MQ=255)
                                    aCGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCGGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGca                                                                 >  2:2153090/1‑100 (MQ=255)
                                      gATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGcatc                                                               >  2:3887665/1‑100 (MQ=255)
                                       aTAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGcatca                                                              >  1:2026366/1‑100 (MQ=38)
                                          ggTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATaa                                                           <  2:208209/100‑1 (MQ=38)
                                          ggTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATaa                                                           >  1:1403699/1‑100 (MQ=255)
                                           gTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATaaa                                                          >  1:186087/1‑100 (MQ=255)
                                            tAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAAc                                                         <  2:2507921/100‑1 (MQ=38)
                                            tAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAAc                                                         <  2:274227/100‑1 (MQ=38)
                                             aGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAAcc                                                        >  1:639226/1‑100 (MQ=255)
                                              gCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACca                                                       <  2:1638330/100‑1 (MQ=255)
                                               cAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAAcctc                                                      <  2:237361/100‑3 (MQ=38)
                                               cAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACcac                                                      <  2:1207716/100‑1 (MQ=255)
                                                aaCGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACcaca                                                     >  1:464443/1‑100 (MQ=38)
                                                 ccgccgcCAACACGACCAACGAAGAAGTCATCCCTGTAGGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCacac                                                    >  1:3920616/2‑100 (MQ=37)
                                                 aCGCCGCCAACACGCCCATCTAAGAAGGCAGGGCTGTAGGCACTATCACCACCAACCTCCCGCAACATATCGGTTTAACCCAGTGCATCATAAAcccgac                                                    <  2:498971/100‑5 (MQ=255)
                                                  cgccgcCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACAcc                                                   >  1:2661453/1‑100 (MQ=38)
                                                  cgccgcCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACAcc                                                   >  1:833185/1‑100 (MQ=38)
                                                  cgccgcCAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACAcc                                                   >  1:3389422/1‑100 (MQ=38)
                                                   gtcgccAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCg                                                  >  1:1494640/3‑100 (MQ=38)
                                                   gccgccAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCg                                                  <  2:1812464/100‑1 (MQ=38)
                                                   gccgccAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCg                                                  >  1:1640464/1‑100 (MQ=38)
                                                      gccAACACGACCAACGAAGAAGTCATCGCTGTATGCCGTGTCACCGCCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAg                                               <  2:3659966/100‑1 (MQ=38)
                                                       caaaCACAACAAACGAAGAAGTCATCACTGTATGCAGTATAACCAACCAATACTAACAGCAAAACAGTGTAACCCAGTGCATCATAAACCACACCCTata                                              >  2:2617885/3‑98 (MQ=255)
                                                           cacGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACg                                          <  2:146593/100‑1 (MQ=255)
                                                            gcggccAAAGGAGAAGTCATGCGTGCATGCCGTGTCACCGCCAAACTCAGGCGGCATTTCGGTGCAACGCAGTGCATCATAAACCACACCGTAGTTACgg                                         <  2:2542190/96‑1 (MQ=255)
                                                             cGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGc                                        <  2:2020636/100‑1 (MQ=255)
                                                              gACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGcc                                       >  1:17165/1‑100 (MQ=255)
                                                                ncAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGt                                     >  1:1028911/2‑100 (MQ=255)
                                                                ccAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGt                                     >  1:3043974/1‑100 (MQ=255)
                                                                ccAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGt                                     >  1:1695642/1‑100 (MQ=255)
                                                                 cAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTa                                    >  2:1664152/1‑100 (MQ=255)
                                                                  aaCGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGCGAAACATAAACCACACCGTAGTTACGGCCGTaa                                   >  2:1419864/1‑100 (MQ=255)
                                                                    cGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATc                                 <  2:4335257/100‑1 (MQ=255)
                                                                      aagaagTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGa                               <  2:3831079/100‑1 (MQ=255)
                                                                            tCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAAc                         <  2:3196523/100‑1 (MQ=255)
                                                                              aTCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCa                       >  1:1845839/1‑100 (MQ=255)
                                                                                 gCTGTATGCAGTATCACCACCAAATTCTGGCAGCATACCGGTGTAACCCAGTGCATCATAAACCACACCCCAGTTACGGCCGTAATCGAAAGAACCAAcc                    >  2:4292884/1‑99 (MQ=255)
                                                                                      aTGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGc               >  1:68250/1‑100 (MQ=255)
                                                                                      aTGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGc               >  1:323734/1‑100 (MQ=255)
                                                                                       tGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCg              >  2:4220896/1‑100 (MQ=255)
                                                                                       cgatctATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCg              <  2:1057624/95‑1 (MQ=255)
                                                                                        gCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGt             >  1:435770/1‑100 (MQ=255)
                                                                                           gTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTAtt          <  2:1989969/100‑1 (MQ=255)
                                                                                             aTCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTa        <  2:570647/100‑1 (MQ=255)
                                                                                             aTCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTa        >  1:3717248/1‑100 (MQ=255)
                                                                                              gcaccgcCAAACTCAGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTaa       >  1:3474455/2‑100 (MQ=255)
                                                                                               caccacCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAg      <  2:3806995/100‑1 (MQ=255)
                                                                                               caccacCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAg      >  1:3281414/1‑100 (MQ=255)
                                                                                                accaccAAATTCTGGCAGCATATCGGTGTAACCCGGTGCATTCTGGACCACGTCGTGGTTACGGTCGTAATCGAAAGACACAACGTCCGCGTATTTAAGa     >  1:3532205/1‑100 (MQ=25)
                                                                                                 ccaccaAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGAc    >  1:3163085/1‑100 (MQ=255)
                                                                                                 ccaccaAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACAAACGTCAGCGTATTTAAGAc    >  1:1191488/1‑100 (MQ=255)
                                                                                                   accaAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGAccc  >  2:2453600/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCC  >  NC_000913/986461‑986659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑