BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,296,060	0	C	T	29.0%	525.5 / 269.3	369	intergenic (+266/+376)	gltP/yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand): ref base C (133/129); new base T (49/58); total (182/187)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.27e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 370 total aligned reads displayed.

CTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCC  >  NC_000913/4295961‑4296158

 ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACg                                                                                                   <  1:880027/100‑1 (MQ=33)

  tAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGc                                                                                                  <  1:1208792/100‑1 (MQ=21)

   aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGcc                                                                                                 >  2:2835845/1‑100 (MQ=33)

   aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGcc                                                                                                 <  1:302200/100‑1 (MQ=34)

    aCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCt                                                                                                >  1:1920870/1‑100 (MQ=34)

     cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTg                                                                                               >  2:467792/1‑100 (MQ=18)

      gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGa                                                                                              >  1:1544756/1‑100 (MQ=33)

       cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt                                                                                             <  1:2745127/100‑1 (MQ=34)

        gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATg                                                                                            <  2:799089/100‑1 (MQ=34)

        gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATg                                                                                            <  2:3598823/100‑1 (MQ=34)

        gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATg                                                                                            <  2:4010593/100‑1 (MQ=34)

          cTTCTCAGGTCTACGCCGGACAGCGCAACAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCg                                                                                          <  2:1060554/100‑1 (MQ=11)

          cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCg                                                                                          <  2:2780511/100‑1 (MQ=21)

          cTTATCAAGCCGACGCCATACAGCGCAGATGCGGGATTTAGCGTGATGTTGTAGCTCGGATAATGCGTTTACGCCGCATCGGACATCAATGCCTGATGCg                                                                                          <  2:2128936/100‑1 (MQ=2)

           ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGa                                                                                         >  2:1472075/1‑100 (MQ=21)

            tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAc                                                                                        <  1:2222038/100‑1 (MQ=21)

            tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAc                                                                                        >  2:373892/1‑100 (MQ=21)

            tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGAc                                                                                        <  1:1366344/100‑1 (MQ=34)

            tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGAc                                                                                        >  1:2916889/1‑100 (MQ=34)

             aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACg                                                                                       >  1:2151369/1‑100 (MQ=34)

              tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGc                                                                                      >  2:1795293/1‑100 (MQ=21)

              tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGc                                                                                      <  1:2001705/100‑1 (MQ=34)

                aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCtt                                                                                    >  1:448469/1‑100 (MQ=21)

                 ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTg                                                                                   >  1:2190201/1‑100 (MQ=25)

                 ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTg                                                                                   >  1:4370884/1‑100 (MQ=21)

                 ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTg                                                                                   >  2:2968256/1‑100 (MQ=34)

                  gCCTACGGCAGACAGCGCAATAGCCTGATGTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGc                                                                                  <  1:2057559/100‑1 (MQ=11)

                  gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGc                                                                                  >  1:55227/1‑100 (MQ=255)

                  gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGc                                                                                  >  2:3469798/1‑100 (MQ=255)

                   ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGcc                                                                                 <  2:1932603/100‑1 (MQ=255)

                   ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGcc                                                                                 <  1:2347235/100‑1 (MQ=255)

                    cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcg                                                                                <  1:1463819/100‑1 (MQ=39)

                     tACGCCAGACAGCGCAATAGCCTGATTTATCGTGATTTTGTATGTAGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCGTGCcgc                                                                               >  2:700903/1‑100 (MQ=11)

                       cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt                                                                             <  2:2737417/100‑1 (MQ=255)

                        gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc                                                                            <  2:3827148/100‑1 (MQ=255)

                        gCCAGACAGCGCAATAGCCTGAGTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTc                                                                            <  2:2535363/100‑1 (MQ=255)

                         ncAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCCACGCCTGATGCGACGCTTGCCGCGTCt                                                                           >  2:3307860/2‑100 (MQ=255)

                         ccAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCt                                                                           <  1:2112279/100‑1 (MQ=255)

                         ccAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCt                                                                           >  1:2423599/1‑100 (MQ=255)

                         ccAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGATTACGCCGCATCCGACATCAGCGCCTGAGGCGACGCTTGCCGCGTCt                                                                           >  2:2445577/1‑100 (MQ=255)

                            gACAGCGCAATAGCCTGATTTAGCGGGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                        <  1:1795202/100‑1 (MQ=255)

                               aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                     <  1:4221759/100‑1 (MQ=255)

                                gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCACATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                    <  2:2897962/100‑1 (MQ=255)

                                 cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                   >  2:412767/1‑100 (MQ=255)

                                 cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                   <  1:3093697/100‑1 (MQ=255)

                                  gcAATGGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                  <  2:318948/100‑1 (MQ=255)

                                  gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                  <  1:3583189/100‑1 (MQ=255)

                                    aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa                                                                <  1:1658224/100‑1 (MQ=255)

                                     aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc                                                               <  1:4165876/100‑1 (MQ=255)

                                     aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc                                                               <  1:2870416/100‑1 (MQ=255)

                                       aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatct                                                             <  2:964354/100‑4 (MQ=255)

                                        gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGcc                                                            <  1:332345/100‑1 (MQ=255)

                                        gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTAGTCAGGCCTAcccc                                                            >  2:2081870/1‑100 (MQ=255)

                                         ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctta                                                           <  1:825191/100‑6 (MQ=255)

                                          cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAg                                                          <  1:778140/100‑1 (MQ=255)

                                          cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAg                                                          <  1:2963116/100‑1 (MQ=255)

                                            gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGAc                                                        <  1:2634332/100‑1 (MQ=255)

                                              tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAg                                                      >  1:1706567/1‑100 (MQ=255)

                                                 aGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcgc                                                   <  2:1360096/100‑1 (MQ=255)

                                                  gCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCGTCAGCCAGCGCa                                                  >  2:3095615/1‑100 (MQ=255)

                                                    gTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATGTTAGCCAGCGCAAt                                                >  2:2290224/1‑100 (MQ=14)

                                                     tggttttGTAGAACGGATAAGGCGTTTACGCCGTGTCCTAGATCAGCGCCTGATACGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATa                                               <  2:4039025/97‑1 (MQ=11)

                                                      gATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAg                                              <  1:995622/100‑1 (MQ=255)

                                                        ttttGTGGGTCGGGTAAGGCGTTTACGCCGCATCCGAGATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGcc                                            <  2:2808776/100‑1 (MQ=255)

                                                        ttttGTAGGTCGTATCAGGGGTTTACGCCGCGGCTGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGcc                                            <  2:2271661/100‑1 (MQ=14)

                                                        atttGTAGTTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGcc                                            <  1:3320127/99‑1 (MQ=255)

                                                         tttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCt                                           <  1:1165618/100‑1 (MQ=255)

                                                          ttttAGGTCGTATAAGTCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTg                                          <  1:2538732/97‑1 (MQ=255)

                                                          ttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTg                                          <  2:3169741/100‑1 (MQ=255)

                                                           tGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGGGCAATAGCCTGa                                         >  1:4077171/1‑100 (MQ=255)

                                                           tGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGa                                         >  2:1328539/1‑100 (MQ=255)

                                                           tGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGa                                         >  1:1049094/1‑100 (MQ=255)

                                                           tGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGa                                         <  1:587689/100‑1 (MQ=255)

                                                                gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAg                                    >  1:1574398/1‑100 (MQ=255)

                                                                atCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATGCCAGCCAGCGGATTATTCTGCTTTAg                                    >  2:874532/2‑100 (MQ=14)

                                                                  cGGATAAGGCCTCGACGCCGCATCGGACATCAACGCCTGATGCCACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCCGCTTTAGCg                                  <  2:3576247/100‑1 (MQ=14)

                                                                       aaGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtt                             <  1:2848683/100‑1 (MQ=255)

                                                                         ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtttt                           >  1:686925/1‑100 (MQ=255)

                                                                         ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAAAAGCCTGATTTAGCGTGAtttt                           >  2:1175189/1‑100 (MQ=255)

                                                                         ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCAGCCAGCGCCATAGCCTGATTTCGCGTCAtttc                           >  2:574416/1‑99 (MQ=14)

                                                                         ggCGTTTACGCCGCATCCGACATCAACACCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtttt                           >  1:4271753/1‑100 (MQ=255)

                                                                           cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                         >  2:435692/1‑100 (MQ=255)

                                                                           cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCATACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                         <  2:3608357/100‑1 (MQ=255)

                                                                            gTTTCCGCCGCATCAGACATGAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa                        <  2:420777/100‑1 (MQ=255)

                                                                            gTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa                        <  1:3789943/100‑1 (MQ=255)

                                                                            gTTTACGCCGCATCCGACATCAACGCCCGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATCGCCTGATTTAGCGTGATTTTGTa                        <  1:3205552/100‑1 (MQ=255)

                                                                            gTTTACACCGCATCCGACGTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa                        >  1:3681817/1‑100 (MQ=255)

                                                                             tttACGCCGCCTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAg                       <  2:2338587/100‑1 (MQ=255)

                                                                                aCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc                    <  2:1680451/100‑1 (MQ=255)

                                                                                 cgccgcATCCGACATCAACGCCTGATGCGACGCTTGCTGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCg                   <  2:3935833/100‑1 (MQ=255)

                                                                                 cgccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATGGCCTGGTTTAGCGTGATTTTGTAGGTCg                   >  1:4014850/1‑100 (MQ=255)

                                                                                    cgcATCCGACATCAACGCCTGATGCGACACTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCCCAATACCCTGATTTAGCGTGATTTTGTAGGCCGCAt                >  2:1529654/1‑100 (MQ=11)

                                                                                        tCCGACATCAACGCCTGATGCGACGCTTGCCGCGCCTTATCAGGCCTAACCTAGAAAGCGCCATAGCTACATCTAGCGTGATTCGGTAAGTCGGATAAga            >  2:661980/1‑99 (MQ=2)

                                                                                         ccGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGc           <  2:2320309/100‑1 (MQ=38)

                                                                                            gcATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGtt        <  2:637315/99‑1 (MQ=37)

                                                                                            aCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGtt        <  2:2029185/100‑1 (MQ=38)

                                                                                              aTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTa      <  1:1119552/100‑1 (MQ=37)

                                                                                               tCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAt     <  1:860251/100‑1 (MQ=35)

                                                                                                cAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGAGCAATAGCCTGATTTAGCGTGATTTTGTAGGGCGGATAAGGCGTTTaag    >  1:3993569/1‑98 (MQ=11)

                                                                                                  aCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTATCGTGATTTTGTAGGTCGGATAAGGCGTTTACGcc  >  2:4094863/1‑100 (MQ=12)

CTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCC  >  NC_000913/4295961‑4296158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`