Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,151,6860GT51.5% ‑5.0 / 22.3 33intergenic (+40/+25)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (12/4);  new base T (9/8);  total (21/12)
Fisher's exact test for biased strand distribution p-value = 2.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCG‑G‑TTGAATTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCC‑GGGCTGGACA‑AAAACTGCTTTAAC‑AATTCA‑GGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATT‑GAAAAACGGGGTA‑AAGAAAGG‑CACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGC  >  NC_000913/2151579‑2151906
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ttGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCG‑G‑TTTAATTTTTT‑TCTT‑TAA‑CCGCCCTTAGAAACTCAATCTGCCCACAGCGCCCGTGCCACAGTTTTTTCATCATCCGTACCCCCCCTTATCCCC‑CGGCTGGACA‑AAAAATGCTTTAACAAATTAG‑GGAAATAAATCAAgt                                                                                               >  2:1280289/1‑239 (MQ=255)
 tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCG‑G‑TTGAATTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAACGCCAGTGCCCACGtttttt                                                                                                                                                                          >  1:1236309/1‑164 (MQ=255)
  gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCG‑G‑TTGAATTTTTT‑TCTT‑TTA‑CCGCCCTGAGAAac                                                                                                                                                                                                             >  1:2335928/1‑130 (MQ=255)
  gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCG‑G‑TTGAATTTTTT‑TCTT‑CCA‑ACGCCCAGAGAAAATCACTCTTCCCCCACCGCCCGTGGCCACGGTTTTTCAACATCCCTACGCTCGTTTACCCCC‑CGGCTGGACA‑AAAAATGCTTTTAA‑ACATTA‑GGTAATAAATCAAgttgt                                                                                            >  2:1180809/1‑239 (MQ=255)
  gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCG‑G‑TTGAATTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCC‑CGGCCGGACA‑AAAACTGCTTTAAC‑AATTCA‑GGTAATAAAACCAgttgt                                                                                            >  1:1642386/1‑239 (MQ=255)
         tATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCG‑G‑TTTGATTGTTG‑TTTT‑TAA‑CCCCCATGAGAAAATCACTCTTCCCACCGAGCCCGTGGCCACGTTTTTTTATTATCCGTACGCTCGTTTTCTCCC‑CGGCCGGACC‑ACAAATCCTTTAAC‑AATTCACGGTATAAAATC‑AGTTGTTCCcgcg                                                                                     >  2:1375740/1‑239 (MQ=255)
              gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCG‑GCTTGAA‑TGTTT‑TTTT‑TAA‑ACCCCAAGAGAAACTCAACTTCCCCCCCACGCCCGTGGCCACGTGCTTTTAATAACCCCACCCCCGTTTTCCCCC‑CGGCTCGTCC‑AAAACCGCCTTTAC‑AATTTC‑CGTAATAAATCTAATTTTTCTTGCGTcgct                                                                                >  2:1215874/1‑239 (MQ=255)
              gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCG‑G‑TTTAATTTTTT‑TTTT‑CAA‑CCGCCTTTAGAAACTCATCCTTCCCCCAACTACCGTGCCTAACCTTTATCATCAACCTTACCCCTCCTTCCCGCC‑GGGCTTGACA‑AAAACTGCTTTAAC‑CAATCC‑GGGAATAAAGACAGTTGTTTCTg                                                                                       >  2:104845/1‑232 (MQ=255)
              gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCG‑G‑TTGAATTGTTG‑TTTT‑TAA‑CCGCCAAGAGaaa                                                                                                                                                                                                              >  1:848699/1‑117 (MQ=255)
              gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCG‑G‑GTGAATTGTTT‑TCTT‑CCA‑ACCCCAGTTGAAAATCAATCTGCCCACCACGCCAGGCGCCACCTTTTATCATCATCCGTACGCTCGCTTACAGCC‑GGGCGGGACA‑AAAAAGGCTTTAAC‑AATTAA‑CGTACTAAATTCAgtcgtttccgcat                                                                                    >  2:1936973/1‑228 (MQ=255)
                                            cTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCG‑G‑TTGAATTTTTT‑TTTT‑CAA‑CCCCCATTGGAAAATCAATCTGCCCCCATCGCCAGTGCCAACGTTTTATACTCCTCCTGTCGCTCGTTTACCCCC‑CGGCCGGGCA‑AAAAACGCTTTAAC‑AATTCA‑GGGAATAAATCACATTGTGTCTGTGGTTTTTCCCCCCACCCATTAGAAAAACGGGGTa‑aa                                                 >  2:458491/1‑239 (MQ=255)
                                            atAAGAGAAAAATTATTTCGAAAATCGGCCTTTATATTTATTGTTAAACCTGGGG‑T‑TTGATTTTTTG‑TTTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCC‑GGGc                                                                                                                                           <  1:970500/152‑1 (MQ=255)
                                              aaGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCG‑G‑TTGAATTGTTG‑TTTT‑TAA‑CCGCCATGAGAAAATCAATCTGCCCACAACCCCAGTGCCAAAGGTTTATTATTATTCCCACGGTCGTTTCTCGCCTGGGCGGGAAA‑AAAATGCCTTAAAC‑AATCAA‑GGtataa                                                                                                       >  2:507178/1‑182 (MQ=255)
                                                     aaaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCG‑G‑TTGAATTTTTT‑TCTT‑CAA‑CCCCCATTAGAAAATCACTCTGCCCCCAGCGCCAGGGGCAACGTTTTTTTATCATCCCTACGCTCGTTTACCGCC‑CGGCGGGGCA‑AAAACTGCTTTAAC‑AAATTA‑GGGAATAAATCGAGTTGTTCCTGAGTTGCTGCCGCCACTCCAAT‑GAAAAAAGGGGTA‑AAGAAAAG‑caca                                      >  2:367181/1‑239 (MQ=255)
                                                      aaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCG‑G‑TTGAATTGTTT‑TTTT‑TAA‑CCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGGTTTTTCATCAACCCTACGCCCGTTTTCCGCC‑GGGGGGGACa‑aaaa                                                                                                                                >  2:970500/1‑153 (MQ=255)
                                                           gTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCG‑G‑TTGAATTTTTT‑TCTT‑CAA‑ACGCCCTGAGAAAATCACTCTTCCCACAGCGCCAGTGCCAAAGATTCATTATCTACCCTACACCCCTTTTCCACC‑CGGCGGGGGA‑AAAAATGCGTTTAC‑AAATTA‑GGTAATAAAATGAGTGTTTCTTGATGGTCTGGCGCCAGCCCACT‑TAAAAACGGGGTAGAAGAAACG‑‑ACATgagga                                >  2:1871307/1‑239 (MQ=255)
                                                                 tAATCAGCCTTTATTTTTTTTTTTAAAACGGGGG‑T‑TTGAATTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGtt                                                                                                                                                                              <  1:246099/98‑1 (MQ=255)
                                                                   atccccctttATTTTTTTTTTTTAAACGGGGG‑T‑TAAAATTTTTT‑TCTT‑CAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCC‑GGGCTGGACA‑AAAACTGCTTTAAC‑AATTCA‑GGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATT‑GAAAAACGGGGTA‑AAGAAAGG‑CACATGAGGAGAATTATc                        <  1:2108269/231‑1 (MQ=255)
                                                                   aTCACCCTTTATTTTTTTTGTTAAAACGGGCG‑T‑TGAAATTGTTG‑TCTT‑AAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCC‑GGGCTGGACA‑AAAACTGCTTTAAC‑AATTCA‑GGTAATAAATCGAg                                                                                                <  1:606614/170‑1 (MQ=255)
                                                                        cttttATTTTTTTTGTTAAACCGGGGG‑T‑TTGATTTTTTG‑TCTC‑CAC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCC‑GGGCTGGACA‑AAAACTGCTTTAAC‑AATTCA‑GGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATT‑GAAAAACGGGGTA‑AAGAAAGG‑CACATGAGGAGAATTATCCAGc                    <  1:1375740/235‑1 (MQ=255)
                                                                        ccGTTATAATTATTGTTTAAACTGGCG‑G‑TTGAAttgtt                                                                                                                                                                                                                                      >  2:2254363/1‑38 (MQ=255)
                                                                         ttttATATTT‑TTGTTTAAAGTGGGGTG‑TTGATTTTTTT‑TCTT‑CAC‑CCGCCATGAGAAACTCACGCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCC‑GGGCTGGACA‑AAAACTGCTTTAAC‑AATTCA‑GGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATT‑GAAAAACGGGGTA‑AAGAAAGG‑CACATGAGGAGAATTATCCAGc                    <  1:1419041/235‑1 (MQ=255)
                                                                           ttATTTTTTTTTTTAAACCGGGGG‑G‑TTAATTTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCcac                                                                                                                                                                                                <  2:659808/70‑1 (MQ=255)
                                                                           ttATATTTATTGTTTAAACTGGCG‑G‑TTGAATTTTTT‑TTTT‑CAA‑CCGCCCTTAGGAAATCACTCTGCCCCCAGCGCCAGTTCCCACCTTTTttcatcat                                                                                                                                                                    >  2:246099/1‑98 (MQ=255)
                                                                             atatTTATTGTTTAAACTGGCG‑G‑TTGAATTGTTGTTTTT‑CAA‑CC‑CCCTGAGAAAATCACTCTTCCCACAGCGCCAGTGCCAACGTTTTTTCATCATCCGTACGCCCGTTTTCCGCC‑GGGCTGGACA‑AAAACTGCTTTTAC‑AATTCA‑GGGAATAAAACGAGTTGTTCCTGc                                                                                      >  2:606614/1‑170 (MQ=255)
                                                                               tttttttttTAAAACGGGGG‑G‑TTG‑ATTTTTT‑TCTTACAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCC‑GGGCTGGACA‑AAAACTGCTTTAAC‑AATTCA‑GGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATT‑GAAAAACg                                                         <  1:1488634/195‑1 (MQ=255)
                                                                                ttttttttttAACCGGGGG‑T‑TTGATTTGTTG‑TCTC‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcatc                                                                                                                                                                      <  1:1362926/91‑1 (MQ=255)
                                                                                     tGTTTAAACTGGCG‑G‑TTTAATTTTTG‑TCTT‑CAA‑CCGCCCTGAGAAAATCACTCTGCCCCCAGCCCCCGTGCCAACGTTTTTTTATCATCCGTACGCTCGTTTACCGCC‑GGGCTGGACA‑AAAAATGCTTTAAC‑AAtt                                                                                                                 >  1:43929/1‑136 (MQ=255)
                                                                                     tGTTTAAACTGGCG‑G‑TTGAATTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCCCAGCGCCCGTg                                                                                                                                                                                      >  1:659808/1‑70 (MQ=255)
                                                                                      ttttAAACGGGGG‑G‑TTTAATTTTTT‑TTCT‑CAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCC‑GGGCTGGACA‑AAAACTGCTTTAAC‑AATTCA‑GGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATT‑GAAAAACGGGGTA‑AAGAAAGG‑CACATGAGGAGAATTATCCAGCATATTTAACGCCAg      <  1:1603026/238‑1 (MQ=255)
                                                                                      tgttAAAAGGGGG‑G‑TTGAATTTGTT‑TCTT‑CAATCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCC‑GGGCTGGACA‑AAAACTGCTTTAAC‑AATTCA‑GGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATT‑GAAAAACGGGGTA‑AAGAAAGG‑CACATGAGGAGAATTATCCAGCATATTTAACGCCAg      <  1:536202/237‑1 (MQ=255)
                                                                                      gTTTAAACTGGCG‑G‑TTGAATTGTTG‑TCTT‑CAA‑CCCCCCTGAGAAAATCCATCTCCCCACAGCGCCCGTGCCAACGCTTTTTCCTCATCCGTACGCGCGTTTTCCGCC‑GGGCTGGACACAAAATTCCTTTAAA‑AATT‑A‑GGTGATAAATGCAGTTGTTCCTGCGTTGATGCCGCCACTCACTT‑TAAAAAAGGGGGA‑AAGAAAGGACACA‑GAGGGGAATTTTCCAGCATATTTAActgcaac     >  2:2010500/1‑233 (MQ=255)
                                                                                          aaaCTGGCG‑G‑TTGAATTGTTG‑TCTT‑TAA‑CCGCCCTGAGAAAATCACTCTTCCCCCAGCGCCCGTGCCAACGTTTTATCATCATCCGTACGc                                                                                                                                                            >  2:1362926/1‑91 (MQ=255)
                                                                                          aaaCCGGGG‑G‑TTGAATTTTTT‑TTCT‑CAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCC‑GGGCTGGACA‑AAAACTGCTTTAAC‑AATTCA‑GGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATT‑GAAAAACGGGGTA‑AAGAAAGG‑CACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGc  <  1:833681/239‑1 (MQ=255)
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TTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCG‑G‑TTGAATTGTTG‑TCTT‑CAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCC‑GGGCTGGACA‑AAAACTGCTTTAAC‑AATTCA‑GGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATT‑GAAAAACGGGGTA‑AAGAAAGG‑CACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGC  >  NC_000913/2151579‑2151906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑