Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913417,5330TG43.2% 33.8 / 14.3 46V131G (GTG→GGG) phoBresponse regulator in two‑component regulatory system with PhoR
Reads supporting (aligned to +/- strand):  ref base T (19/6);  new base G (18/1);  total (39/7)
Fisher's exact test for biased strand distribution p-value = 1.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAG‑AATTTAAACTG‑‑CT‑GCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGC  >  NC_000913/417307‑417768
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                
ttACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGCAGAGGGGATTGAGAA‑Gcg                                                                                                                                                                                                                                     >  1:1743237/1‑238 (MQ=255)
       gcggcTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGAGGGAGGGGTGGTGTGGAT‑GGAGGGGTTa                                                                                                                                                                                                                              >  2:2578277/1‑239 (MQ=255)
        cggcTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGCATTTCGCCAATGGCGGTGGAAGAGGTGATTga                                                                                                                                                                                                                                            >  1:2190748/1‑225 (MQ=255)
        cggcTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGGGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGCGGAAGAGGGGATTGAGAG‑GCAGGGATTaa                                                                                                                                                                                                                             >  1:2767858/1‑239 (MQ=255)
          gcTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGGGGCGCGAATCAAAGCGGTAATGCGCCGTCTTTCGCCAATGGCGGTGGGAAGGGGGATTGCGGT‑GCAGGGGTTTAg                                                                                                                                                                                                                            >  2:2085452/1‑238 (MQ=255)
            tCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGAAGAGGGGGTTGTGAT‑GCAGGGGTTAAGtc                                                                                                                                                                                                                          >  2:885865/1‑238 (MQ=255)
             ccGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGGGCGAATCAAAGCGGTAATGCGCCGGATTTCGCCAATGGCGGGGGAAGAGGAGAATGGGCT‑GCAGGGAGTGAGtctc                                                                                                                                                                                                                        >  2:246259/1‑239 (MQ=255)
                        ttCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGAAGAGGGGGTTGAGGT‑GCAGGGATTAAGGCTCGACCCGACa                                                                                                                                                                                                               >  1:1728961/1‑237 (MQ=255)
                           atcaAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGAAGAGGGGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACAtctc                                                                                                                                                                                                           >  1:2211175/1‑238 (MQ=255)
                                  acCTCGAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGTGATTGAGAT‑GCGGGGATTAAGTCTCGACCCGACATCTCACCGAGTg                                                                                                                                                                                                   >  2:1733951/1‑239 (MQ=255)
                                  acCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGGCGAGGGGCTTGAGAA‑GCGGGGATTTAGTCTCCAACCGCCAGCCCACCggggg                                                                                                                                                                                                   >  2:1362758/1‑235 (MQ=255)
                                     tCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGGAGAGGGGATTGTGAT‑GCAGGGGTTAAGACTCGACCCGACCTCTCCCCGGGCGATg                                                                                                                                                                                                >  2:888734/1‑239 (MQ=255)
                                      cAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACCGAGt                                                                                                                                                                                                    >  1:1681247/1‑234 (MQ=255)
                                              aGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGGTTTAGAT‑GCAGGGAATAAGTCTCGGCCCGACAGTTCACCGCGTGaacgctgggggt                                                                                                                                                                                       >  2:2331769/1‑228 (MQ=255)
                                              aGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGGGTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACCGAGGGAGGGCGGGGGaa                                                                                                                                                                                       >  2:2501460/1‑239 (MQ=255)
                                              aGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGaa                                                                                                                                                                                       >  1:1443767/1‑239 (MQ=255)
                                              aGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACCGAGGGATGGCGGGCGaa                                                                                                                                                                                       >  1:41992/1‑239 (MQ=255)
                                                                     gtggtgATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACCGAGGGATGGCGGGCGGAGAGCCGCGGGGGATGGGGCCggc                                                                                                                                                                >  1:2448714/1‑237 (MQ=255)
                                                                     gtggtgATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGAAGAGGTGATTGAGAT‑GCAGGGATTAAGGCTCGACCCGACATCTCACCGAGGGATGGCGGGGGAAGAGCCGCCGGGGATGGGGGCggc                                                                                                                                                                >  1:2361023/1‑237 (MQ=255)
                                                                      tggtgATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGGCa                                                                                                                                                               >  1:1708928/1‑239 (MQ=255)
                                                                      tggtgATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGGAGAGGGGATTGGGGTGGCGGGAATAAAGTCTC‑ACCCGACCTTTTCCCGAGGGTGAGGGGGCGACGAGGCGCTGGGGGTGGGGGCgccc                                                                                                                                                               >  2:2366175/1‑236 (MQ=255)
                                                                         tgATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCa                                                                                                                                                                                                          >  1:1228849/1‑193 (MQ=255)
                                                                           aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGGGGAGAAGCCCTGGGAGTGGGGGCGGCAG‑AAtt                                                                                                                                                         >  1:1505526/1‑239 (MQ=255)
                                                                             gTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGAAGAGGGGATTGAGAT‑GCAGGGATTAAGTCTCGAACCGACATCTCACCGAGGGATGGCGGGGGAAGAGCCCCTCGGGATGGGGCCGACAG‑AAattt                                                                                                                                                       >  1:306378/1‑238 (MQ=255)
                                                                                gACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGAT‑GCAGGGATTaa                                                                                                                                                                                                                             >  1:2643165/1‑167 (MQ=255)
                                                                                  ccGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGGGAAGAGCCGCCGGGGAAGGGGGCGGCCG‑AATTTaa                                                                                                                                                      >  1:2189493/1‑235 (MQ=255)
                                                                                   cGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGGGCAGAGCCGCCGGGGAGTGGGGCGGCAG‑AATTTTAAct                                                                                                                                                   >  1:1020268/1‑237 (MQ=255)
                                                                                   cGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAG‑AATTTAAAct                                                                                                                                                   >  1:379544/1‑237 (MQ=255)
                                                                                     ccAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGAT‑GCCGGGATTAAGTCTCGACCCGACATCTCACCGAGGGATGGCGGGCGAAAAGCCGCTGGGGAAGGGGGCGCCCG‑AAATTaaaatcgc                                                                                                                                                >  1:1875300/1‑233 (MQ=255)
                                                                                             gaagaagaaGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGGGAAAAGCCCCTggg                                                                                                                                                                            >  1:1058908/1‑202 (MQ=255)
                                                                                                           cgTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGGTTGAGAG‑GCAGGGATTAAGTCTCGAACCGGCATCTACCCGAGGGATGGCGGGCGGAGAGCCCCCGGGGAGGCGGGTGGCAG‑AAGTTTAACAGGTCT‑GCACttttttgacgcggcctc                                                                                                                        >  2:2178598/1‑228 (MQ=255)
                                                                                                                                   ggatagctgtatCAGCAATCCGTTTTCGCGGAAGGAGCTGGTGGGGCGAATCAAAGCGGTAATGCGCCGTTTTTCGCCAATGGCGGTGGAAGAGGTGATTGAGAT‑GGATGGATTAAGTCTCGACCCGGCATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGGGATGGGGCCGACAG‑AATTTAAACTG‑‑CT‑GCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGagcag                                                                                               <  2:1703147/229‑1 (MQ=255)
                                                                                                                                   ggATGACAATATCACCGAGCCGTTTTCGCAGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAAGGCGCCGTATTTCGCCAAGGGCGGTGGAAGAGGGGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAG‑AATTTAAACTG‑‑CT‑GCCCTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGagcag                                                                                               <  2:1743237/238‑1 (MQ=255)
                                                                                                                                                 ccAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGAT‑GCAGGGATTAAGTCTCGACCCCACATCTCACCGAGTGATg                                                                                                                                                                                                >  1:79441/1‑131 (MQ=255)
                                                                                                                                                    aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGAT‑GCAGGGGTTAAGTCTCGACCCGACATCCCCCCGAGTGATGGACGGGGGAGAAGCGCCGGGGATGGGGGCCCCAGCAATTTAAAC‑G‑‑TT‑GCACCTTTTTTTGGAGCCTCCCGAGCGCGCGTTCCACCGCCGGCAGCTGTTTaacaaccgcccg                                                                             >  2:830366/1‑233 (MQ=255)
                                                                                                                                                    aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACCGAGt                                                                                                                                                                                                    >  1:542421/1‑124 (MQ=255)
                                                                                                                                                    aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGGAGAGGTGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACCGAGGGATGGGGGGGGGGAAGGCGGCGGGGACGGGGGCGGCAG‑AAATTAAACAC‑‑CG‑GGCCTTTTTTTTTTAGCATCCTGTGCGCGTGTATAGCCTCGAGCGGCTGTTAAAACACGTCTgg                                                                             >  2:285334/1‑239 (MQ=255)
                                                                                                                                                         ttttCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGAGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACCGGGTGATGGCGGGGGGAAAGCCCCCGGGGGTGGGGCCGACAG‑AATTTTAAATG‑‑CTGGCCATTTTTTAT‑ACGCCTCCCGCGGGCGCGGACAACCACCGGGGCCTGTTAAACCCCCTCTgcggg                                                                          >  2:1572880/1‑236 (MQ=255)
                                                                                                                                                             cGCCGAGGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAGGAGGTGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACGGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAG‑AATTTAAACTG‑‑CT‑GCTCTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAAc                                                                    <  2:1409166/239‑1 (MQ=255)
                                                                                                                                                                        cTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCCCCGAGTGATGGCGGGGGGAGAGACGCCGGGGATGGGGCCCCCAC‑AATTTTAAATT‑‑CT‑GCACTTTTTTTTTAAGCCTCCTGTGCGCGGGTGTAGCCCCGCGCAGCGGTGTAAACACGGCTGGGGGAATAAAGTGTAtgtgtg                                                         >  2:123096/1‑237 (MQ=255)
                                                                                                                                                                                          gCGGTAATGCGCCGTATATCGCTAAGGGCGGTGGAAGAGGTGATTGGGAT‑GCGAGGATTAAGTCTCGACCCGACATCTCACCAAGTGATGGCGGGCGTAGAGCCGCTGGAGATTGGGCCGACAG‑AATTTAATCTG‑‑CT‑GCACTTTTTTATGGCGCGTCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCCCGGTCg                                           <  2:306378/235‑1 (MQ=255)
                                                                                                                                                                                                                  aTGGCGGTGGAAGAGGTGATTga                                                                                                                                                                                                                                            <  2:2172326/23‑1 (MQ=255)
                                                                                                                                                                                                                  aTGGCGGTGGAAGAGGTGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACCGAGt                                                                                                                                                                                                    >  1:1683825/1‑62 (MQ=255)
                                                                                                                                                                                                                      cGGTGGAAGAGGTGATTGAGAT‑g                                                                                                                                                                                                                                       <  2:1039422/23‑1 (MQ=255)
                                                                                                                                                                                                                      cGGTGGAAGAGGTGATTGAGAT‑g                                                                                                                                                                                                                                       <  2:2643165/23‑1 (MQ=255)
                                                                                                                                                                                                                               agGTGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAG‑AATTTTAAATG‑‑CT‑GCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCCAGCAGCTGTTAAACCACGGCTGGGGAACTAAAGGGTATGGGGAAAACCGCCCGGTCTATGTCCACATTCGTCGCCTGAGTAAAGCACGGGACACcggc  >  2:1641384/1‑239 (MQ=255)
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                
TTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGAT‑GCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAG‑AATTTAAACTG‑‑CT‑GCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGC  >  NC_000913/417307‑417768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑