Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,151,6860GT50.0% ‑6.1 / 12.9 24intergenic (+40/+25)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (8/4);  new base T (7/5);  total (15/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑ACAAAAACTGCTTTAACAATTCA‑GGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGT‑CCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTA  >  NC_000913/2151448‑2151921
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               
tATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑ttg                                                                                                                                                                                                                                                 <  1:960648/239‑1 (MQ=255)
                      ccTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑ttg                                                                                                                                                                                                                                                 <  1:1105279/217‑1 (MQ=255)
                                                                                                                                gctttGCTCTTTTATTGCACCCCTACAAAAAAAAAGAAGCCGAAGGGCAAAAAAAAAAATTATTTCTTAAATCAGCCTTTATATTT‑TTTTTTAAACCGGGGGTTTAA‑TTTTTTTTTTCCACCCGCCATGAGAAACTCACTCTg                                                                                                                                                                                                                <  2:1639114/140‑1 (MQ=255)
                                                                                                                                    tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTGTTTTTTT‑TAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGAGCCAACGATTTTTCCTCAACCGTACCCCCGTTTACCCCCCGGCTGG‑ACAAAAACTGCTTTATCACATAA‑CGGAATAAAACGa                                                                                                              >  2:1367637/1‑235 (MQ=255)
                                                                                                                                    tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTT‑CAACCGCCATGAGAAactcact                                                                                                                                                                                                                   >  1:187819/1‑136 (MQ=255)
                                                                                                                                           ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTTTTTTTTT‑CCACCCCCCTGAGAAACTCACTCTCCCCACAACGCCAGTGCCACCGTTTTTTTATCATCCGTACGCCCCCTTTCCCCCCGGCTGG‑GCAAAAAACGCGTTTACAACTCACGGCATTAAACCAAGTGGTTct                                                                                                      >  2:2484274/1‑236 (MQ=255)
                                                                                                                                            tATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTTTTGTCTT‑CAACCGCCATGAGAAAATCACTCTGCCCACAgcgc                                                                                                                                                                                                      >  1:1639114/1‑141 (MQ=255)
                                                                                                                                                 gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTGTTTTCTT‑CAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGGGCCAACGGTTTATTATCATCCGTACGCTCGGTTACCGCCGGGCTGG‑ACaaaaa                                                                                                                                            >  1:1477864/1‑193 (MQ=255)
                                                                                                                                                   cACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGGTGTA‑TTGTTGTCTT‑TAACCGCCCTGAGAAAATCACACTGCGCCCAACGCCCGTGCCAAAGTTTTTTCCTCATCCCTACACCCGCTTTCCGCACCGCTGGCACAAAAAC‑GCTTTTACAATTCT‑GGGAATAAAACGAGGTGTGTCTCGGTtgctt                                                                                            >  2:2246009/1‑237 (MQ=255)
                                                                                                                                                      ttATCAAAAAGAAGAAGCCGACGAGTAAAGAAAAAAATTATTCCGCTAATCAGCCTTTATTTTTTTTTTTAAAACGGGGGGTTAAA‑TTGTTGTCTC‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGcc                                                                                                                                                                                                     <  1:1116651/131‑1 (MQ=255)
                                                                                                                                                                gaagaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTGTTTTCTT‑CAACCGCCATGAGAAAATCACTCTGCCCACACCGCCAGTGCCaaag                                                                                                                                                                                           >  2:1116651/1‑130 (MQ=255)
                                                                                                                                                                   gaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGGA‑ATTTTTTCTT‑TAACCCCCCTGGGAAAATCACTTTGCCCACAGCCTCAGTGCCCACCGTTTATTATCTTACCCGCGCTCGTTTTCCCCCCGGGTGG‑ACAACAAATGCTTTTACAATTCA‑CGGAATTAATCCAGGTGGTTCTTGGTTTGTGCCGCCAGT‑CCAtatgac                                                                          >  2:1489746/1‑234 (MQ=255)
                                                                                                                                                                   gaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTGTTTTCTT‑CCACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTACCCCCCGGCTGG‑ACAAAAACTGCTTTTACAATTCA‑GGTAATAAAACGAGTTGTTCCTGCGTTTCTTCCCCCCGT‑CCAtttaca                                                                          >  2:955347/1‑235 (MQ=255)
                                                                                                                                                                     acccGACGAGAAAAGAAAAAATTTATTTCCCAAACCACCCTTTAT‑TTTTTTTTTTAAACCGGGGGTTTAATTTGTTGTCTT‑CACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑ACAAAAACTGCTTTAACAATTCA‑GGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGT‑CCATTGaaaaa                                                                        <  1:1155579/237‑1 (MQ=255)
                                                                                                                                                                                  agagCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGTA‑GTTTTGTAAT‑TTAACTCCCTGAGGAATACACTCTTCCCCCACAGCCCCTGCCTAAGTTTTATCATCACCCATAACACCCTTTCCCCACCGGCGGG‑CCAAAAAATGCTTTTACAATTCC‑GGAAATAAAACAAATGTTTCCTGCCGTGCTTCCTCCAGTCCCATGCAAAATCAGGGTcaagaa                                                            >  2:2363633/1‑239 (MQ=255)
                                                                                                                                                                                        aaaTTTGTTCCCAAATCAGCCCGTAATTTTTTTTTTTAAACGGGGGGTTGAATTTTTTTTCTC‑AAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑ACAAAAACTGCTTTAACAATTCA‑GGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGT‑CCATTGAAAAACGGGGTAAAGAAAGGcac                                                      <  1:1177758/239‑1 (MQ=255)
                                                                                                                                                                                        aaaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTTGTGTATT‑CTACCGCCATTAGAAACTCACTCTGCCCACAACGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑ACAAAAACTGCTTTAACAATTCA‑GGCAATAAATCGAGGTGTTCCTGCGGTGGTGCCGCCCGT‑CCACTGaaaaa                                                                        >  2:1660601/1‑220 (MQ=255)
                                                                                                                                                                                              gTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTTTTTTTTT‑TCACCGCCCTGAGAAAATCACTCTTCCCACAGCGCCCGTGGCAACCTTTTATTCTCATCCCTACGCTCGTTTACCGCCGGGCCGG‑GCCAAAACAGCttttaa                                                                                                                                  >  2:1276243/1‑155 (MQ=255)
                                                                                                                                                                                                 ctaaaaTCAGCTTTTATATTTTTTGTTAAACCGGGCGTGTGAT‑TTTTTGTTCT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑ACAAAAACTGCTTTAACAATTCA‑GGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGT‑CCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGaa                                            <  1:307505/235‑1 (MQ=255)
                                                                                                                                                                                                      aTCAGCCGTTATATTTATTGTTTAAACTGGCGGTTTAA‑TTTTTTTTTT‑TCACCCCCATGAGAAAATCACTCTCCCCACAAGCCCCGGGCCCACGCTTTTTCATCCCCCCTACGCTCGCTTTACGCCCGGCAGG‑ACAAAAAATGCTTTAAAAATTCA‑CGGAATAAATCCGTTTGTTCCTGCGTTGTTTCCGCCCGT‑CCATTGCAAAAAAGGGGAAAAACAAGTACATTAGGGGAAttta                                        >  2:2080207/1‑236 (MQ=255)
                                                                                                                                                                                                           cttttATTTTTTTTGTATAAGCGGGGGGTTATT‑TTTTTTTCTC‑CACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑ACAAAAACTGCTTTAACAATTCA‑GGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGT‑CCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCa                                  <  1:1489746/236‑1 (MQ=255)
                                                                                                                                                                                                              ttATATTTTTTG‑TTAAACCGGGGGTTTAATTTTTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcatcat                                                                                                                                                                               <  1:2698075/98‑1 (MQ=255)
                                                                                                                                                                                                              ttATATTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTT‑CAACCGCCATgag                                                                                                                                                                                                                            >  1:350050/1‑53 (MQ=255)
                                                                                                                                                                                                                        tGTTTAAACTGGCGGTTGAA‑TTGTTTTTTT‑CAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCCGTGCCCACGGTTTTTTATCATCCCCACGccc                                                                                                                                                                     >  2:2698075/1‑96 (MQ=255)
                                                                                                                                                                                                                        tGTTAAAACGGGCTGTAGAG‑TTGTTTTTTC‑CACCCGCCATGAGAAACTCCCTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑ACAAAAACTGCTTTAACAATTc                                                                                                                             <  1:1642351/137‑1 (MQ=255)
                                                                                                                                                                                                                                     gtttata‑TTGTTGTATA‑CACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACTTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑ACAAAAACTGCTTTAACAATTCA‑GGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGT‑CCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAcc         <  1:1730963/232‑1 (MQ=255)
                                                                                                                                                                                                                                            tttgttcTCTA‑AACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑ACAAAAACTGCTTTAACAATTCA‑GGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGT‑CCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTa  <  1:1894242/238‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               
TATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑ACAAAAACTGCTTTAACAATTCA‑GGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGT‑CCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTA  >  NC_000913/2151448‑2151921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑