| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 354,812 | C→T | intergenic (+220/‑110) | prpE → / → codB | propionate‑‑CoA ligase/cytosine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 354,812 | 0 | C | T | 99.5% | 799.5 / NA | 200 | intergenic (+220/‑110) | prpE/codB | propionate‑‑CoA ligase/cytosine transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (107/92); minor base G (0/1); total (107/93) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 200 total aligned reads displayed.
CGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATG > NC_000913/354579‑355032 | ggAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCata > 2:2506795/1‑239 (MQ=255) aGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATaaa < 2:2982509/235‑1 (MQ=255) ggTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAata > 1:716422/1‑239 (MQ=255) gTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTTTGGGCCCATGCCTGATGCGACGCCGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGGGACGCATCCGACACCAAGCACAGATGCCTGATGCGACGCCAACGCGTCTTATCAGGCCTACCCACTGTTTTTACATCGAAAAATTTTACCCCACCTTTCTGTATTCATTCACATATAAAatat > 2:1516165/1‑239 (MQ=255) tGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATAttt > 1:1169715/1‑238 (MQ=255) tGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATAttt > 1:1225670/1‑238 (MQ=255) tGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATAttt > 1:234771/1‑238 (MQ=255) gcgGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGAtt < 2:2453347/239‑1 (MQ=255) cACGGTGTGCCGTTGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGATCGGATGCCTAATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTGTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCtt < 2:3452989/238‑1 (MQ=255) aCTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCa > 1:3307909/1‑239 (MQ=255) gtgtgCCGATGCCTGATGCGACCCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCGGGCGCCGCATCCGGCACCATCCTCCGATGCCTTATGCGACGGTAACGCGCCGTAGCACGTCTACCCACTGTATGTAAACCGACAATTTTCCCACCCCCTTGTTTCATTCATTCCTATAAAAAATATATTTGCCCACGAAGATGATAGCTTATTATCCTCAGAt > 2:2268357/1‑239 (MQ=255) tgtgCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATg > 1:2485659/1‑239 (MQ=255) tgtgCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATg > 2:3240364/1‑239 (MQ=255) gCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAAt > 1:53359/1‑239 (MQ=255) tGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGaa > 2:3018507/1‑206 (MQ=255) tGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGaa < 1:3018507/206‑1 (MQ=255) ccTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTCCACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATCAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATACAATgc > 2:2307860/1‑239 (MQ=255) cGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGAtttt > 1:1877623/1‑239 (MQ=255) tGACGCGTTTTATCATGCCTACCGACCTGAACCGTAGGGCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGAtttttt < 2:2584200/238‑1 (MQ=255) ttATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCaa > 2:1997903/1‑238 (MQ=255) tATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAAc < 1:2557838/239‑1 (MQ=255) gcgcccgtaCCTGACCCGTGGGTCGGATAAGGCGCCCGCTTCGAATCCTGCGACATGCCCAAATGCCTTATGCGATGCTTACGCCTCTTATCAGGCCTACCCATTGTTTTTACAACGATAATTTTTCCCCCACCTTTTTACATTCATTCATATAAAAAATATATTTCCCCGCAAAACCGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGAtttttt < 2:606628/214‑1 (MQ=255) gCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCaaa > 1:1001163/1‑239 (MQ=255) ccTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTTAAACAGCaaaa > 2:44427/1‑239 (MQ=255) cTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCaaa > 1:606628/1‑222 (MQ=255) aaCCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCtt > 1:3520367/1‑181 (MQ=255) tAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCgtgt > 1:1200842/1‑238 (MQ=255) tAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTc > 1:1557452/1‑239 (MQ=255) tCGGATACGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGc < 2:2077301/237‑1 (MQ=255) tCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGc < 1:2305684/237‑1 (MQ=255) tCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGc < 2:1417389/237‑1 (MQ=255) tAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATa < 1:2456557/238‑1 (MQ=255) aaGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATaa < 2:1001163/238‑1 (MQ=255) gcgcTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATaaa < 1:43676/125‑1 (MQ=255) gcgcTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATaaca > 2:878179/1‑237 (MQ=255) gcgTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGc < 2:2485659/239‑1 (MQ=255) cATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAggg > 1:3278035/1‑238 (MQ=255) aTCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGc < 2:2540567/239‑1 (MQ=255) ccGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCa < 2:1557452/239‑1 (MQ=255) ccATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATtcattcat < 1:3130827/99‑1 (MQ=255) ccATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATtcattcat > 2:3130827/1‑99 (MQ=255) aTGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCa > 2:3506416/1‑231 (MQ=255) gCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGt < 1:2219276/239‑1 (MQ=255) cTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAgg < 1:2685872/186‑1 (MQ=255) aGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCttttt < 2:2138244/129‑1 (MQ=255) aGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATa > 2:1241769/1‑205 (MQ=255) aGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATa < 1:1241769/205‑1 (MQ=255) tGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTATTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAAATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGgcg < 1:1516165/237‑1 (MQ=255) cTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGaa < 1:1403309/239‑1 (MQ=255) cTGATGCGACGCTGACGCGTCTTATCAGGACTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGa < 2:1192779/238‑1 (MQ=255) tGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGa < 2:572116/113‑1 (MQ=255) tGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGa > 1:572116/1‑113 (MQ=255) tGATGCGACGCGTACGAGTCTTATCGGGCCTACCTACTGGTGTTACACCGAGAAAGTTCCCCCCACCTTGTTGCAGTCAGTCATATAAAAAATATATTACCCCGCGAAAACGATTACTTTTTATCTACAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTGCGTGTCGCAGGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGaa < 2:2369927/238‑1 (MQ=255) gATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCtt < 2:1867523/126‑1 (MQ=255) gATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAg < 2:1169715/239‑1 (MQ=255) catgccccacTGCCGCGTATTATCCGGCCTACCCACTGTTTTTACCCGGATAATTTTTCCCCCACCTTTTTACATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCTGTCCCGCAGTCGGCGCGGAAAg < 2:1225670/230‑1 (MQ=255) tGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATaa < 1:3341978/196‑1 (MQ=255) cGCTGGCGCGTCTTATCAGGCCTAACCACTGTATTTACACCAATAATTTTTCCCCCACATTTTTGCATTCATTCATATAAAAAATATATTTCCCCTCGAAAGCGATTGCGTTTGATCATCAGAAGAAAAGAATGCGGCGGATTTTTTGGGTTTGAAACAGAAAAAAGGGAGAATTTGGTGTGGCAAGATAAAAACTTTAGGCAGGGGACAGTCCAGCAGTCGGAGCGCAAAGGGGTAt < 2:234771/238‑1 (MQ=255) cGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGAtttttt < 1:2386407/147‑1 (MQ=255) cGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTAt < 2:3307909/238‑1 (MQ=255) cgcgTCTTATCAGGCCTACGCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGACGGATTTTTTGGGTTTCAAACAACAAAAAGGGGGAAGTTCGTGTCGCAAGATACCAGCTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGATATTGGcgt < 2:1826828/238‑3 (MQ=255) ttATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGtt < 1:2506795/239‑1 (MQ=255) aTCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCg < 1:3240364/239‑1 (MQ=255) tCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGt < 2:2305204/239‑1 (MQ=255) gCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACg > 2:2633265/1‑79 (MQ=255) gCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACg < 1:2633265/79‑1 (MQ=255) gCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGAc > 2:518302/1‑229 (MQ=255) gCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGAc < 1:518302/229‑1 (MQ=255) gCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGTCAt > 2:1906976/1‑238 (MQ=255) ccTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGAt < 1:2907133/86‑1 (MQ=255) cTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGTCATGc < 1:878179/238‑1 (MQ=255) cccACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGAt < 1:2267627/82‑1 (MQ=255) cccACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGAt < 1:2960701/82‑1 (MQ=255) cccACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGAt > 2:2960701/1‑82 (MQ=255) cccACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACt < 1:713735/172‑1 (MQ=255) cccACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACt > 2:713735/1‑172 (MQ=255) tGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCtt < 2:85817/90‑1 (MQ=255) tGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCtt > 1:85817/1‑90 (MQ=255) tGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCtt < 2:2267584/90‑1 (MQ=255) tGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGc > 2:2877262/1‑155 (MQ=255) tttttACCCCGATAATTTTTCCCCCCCCTTTTTGCCTTCTTTCATATAAAAAATATATTTCCCCCCCAAAACGATTGCTTTTTATCTTCAg < 1:655550/91‑1 (MQ=255) ttACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACct > 2:2668470/1‑237 (MQ=255) ccGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCtttt > 1:1688543/1‑238 (MQ=255) gATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAATATTTATTTTCTCACGAAAACGATTGCTTTTTATCTTCAGTTGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGCAAACAACTTTAGCCAGGGACGAGTCTCGCCGTCGACTCGGAACGAGGTATTGGCCTTGACGTTCGTCATGCTGGGATTACGCATCTTTTcc > 2:3285168/1‑238 (MQ=255) gATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCCCGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCaa > 2:2399148/1‑122 (MQ=255) gATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATc < 1:3436861/76‑1 (MQ=255) gATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATc > 2:3436861/1‑76 (MQ=255) gATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATaa > 1:1359209/1‑150 (MQ=255) gATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATaa < 2:1359209/150‑1 (MQ=255) gATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGc > 1:209038/1‑167 (MQ=255) gATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTcc > 2:420996/1‑238 (MQ=255) gATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTcc > 1:2765535/1‑238 (MQ=255) gATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTcc > 1:76616/1‑238 (MQ=255) gATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCg < 1:2449043/239‑1 (MQ=255) tattttttCCCCCCCCTTTTTGCATTCATTCATATAAAAAATATATTGCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGcc < 2:2714290/236‑1 (MQ=255) tAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGcc < 2:76616/239‑1 (MQ=255) aaTTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCa < 1:1857911/239‑1 (MQ=255) cccccACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAgggg < 1:2000194/119‑1 (MQ=255) ccccACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGgcg < 2:1057157/173‑1 (MQ=255) ccccACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGgcg > 1:1057157/1‑173 (MQ=255) | CGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATG > NC_000913/354579‑355032 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |