| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 354,812 | C→T | intergenic (+220/‑110) | prpE → / → codB | propionate‑‑CoA ligase/cytosine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 354,812 | 0 | C | T | 98.6% | 253.1 / ‑4.8 | 71 | intergenic (+220/‑110) | prpE/codB | propionate‑‑CoA ligase/cytosine transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (44/26); minor base G (0/1); total (44/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01 | |||||||||||
CTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGCGGCACTCTCGGAACCGGTCTTAGCTATCATGATTTCTTCCTCGCAGTTCTCAT > NC_000913/354533‑355094 | cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGCTAATTTTTCCCCCACCTTTTTGCATTCATTCata > 2:428668/1‑289 (MQ=255) gacgacCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATtcattcattt > 1:105176/1‑286 (MQ=255) ccTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGCCACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTccc > 2:290905/1‑288 (MQ=255) ccTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCCTGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCCTATAAAAAATATATTTcccc > 2:305355/1‑289 (MQ=255) ccTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTcccc > 1:250310/1‑289 (MQ=255) cgtcgtTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCATACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACg > 1:135864/1‑288 (MQ=255) ttGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAAc > 1:719051/1‑288 (MQ=255) gCCAGGCGATGGACGAGTAGGTTATTGTCGGATGCGTCGCGCGGGGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGCACCTGAGCCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCATATGCCTGATGCGACGCTGACGCGTCTTATCAGGCTTACCCACTGTTTTTACACCGATAATTTCTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATc < 2:94322/289‑1 (MQ=255) gCGATGGAAGAATAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAGATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAg < 1:49228/289‑1 (MQ=255) gATGGAAGAGTAGGTGATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGAt < 2:348319/289‑1 (MQ=255) ggTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATaaa > 2:362634/1‑234 (MQ=255) ggTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATaaa < 1:362634/234‑1 (MQ=255) tATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATgcggcg > 1:337085/1‑289 (MQ=255) tGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGAt > 1:638769/1‑289 (MQ=255) tGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCCCAGATGCCTGATGCGACGCCTACGCGTCTTATCATGTCTACCCCTTGTTTTTACAACGATAATTTCTCCTCCACCTTTTCGCATTTTTTCATATAAAAAATACATTTCCTCACTAAAACGATTGCTTTTTACTTTCAGATGAATCGTATGcggaggct > 2:443214/1‑287 (MQ=255) gATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTCTCTTCAGATGAATAGAATGCGGCGGAtttttt > 1:32432/1‑289 (MQ=255) cATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCGGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCa > 1:315774/1‑288 (MQ=255) cACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAgggg < 2:250310/289‑1 (MQ=255) aCTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGACGCGACGCTGACCCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCATCTTTTTGCATTCATTCAATTAAAAAATATCTTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATCGAATGCGCCGGATTTTTTGGGTTTCAAACTGACAAAAggggg > 2:693622/1‑289 (MQ=255) tgtgtgCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGaa > 2:121362/1‑289 (MQ=255) tgtgCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATaaa < 1:577912/195‑1 (MQ=255) tgtgCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATaaa > 2:577912/1‑195 (MQ=255) tgtgCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAgggg > 2:638049/1‑284 (MQ=255) tgtgCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAgggg < 1:638049/284‑1 (MQ=255) cGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCgtg > 2:571001/1‑289 (MQ=255) tGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCTTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATACATTTCCCCACGAAAACGATTGCTTTTTATTTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCgtgttg > 1:276469/1‑287 (MQ=255) tGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAACGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTGTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCg < 1:211136/289‑1 (MQ=255) gCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGTATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGan > 2:646357/1‑228 (MQ=255) ccggATGGGACGCTGACGGGTTTTATCATGCCTCAGGACCTGAACCGTAGGTCGGGTAAGGCGGTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACGGCAAAAAGGGGGAATTTCGTGTCGCa < 2:491662/286‑1 (MQ=255) ccTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGc < 1:571001/288‑1 (MQ=255) gcgcCGCGGACGCGTTTTCTCGTGCCTACGGACCTGAGCCGTAGGTCGTATAAGGCGCTCGCCTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGCACTGTTTTTACACCGATAATTTTTCCCCCACCTATTTGCATTCATTCATATAAAAAATACATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATaa < 2:276469/289‑1 (MQ=255) gACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATaac > 2:440570/1‑288 (MQ=255) aCGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCCGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTTTCGCAAGCTaacaa > 1:560340/1‑289 (MQ=255) aCGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATaaca > 1:112555/1‑288 (MQ=255) cGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTCTCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCTCCCACCTTTTTGCATTCATTCATATACAAAATACACTTCCCCACGAAAACGATTGCTTTTTATCTTTAGATGAATAGAATGCGGCGGATTTTTTGGGTTGCAAACAGCAAAAAGGGGGAATCTCCTCTTGTAAGATcaccaa > 2:644779/1‑286 (MQ=255) ggtGACGCGTTGGATGATGCCACCGGACCTGAACCGTAGGTCGGGTAAGGCGCCCAGGTCGCATCAGACACCATGCTCAGATTCCTGATGCGAGGCTGAGGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTGTGCATTCATTCATATAAAAAATATATTGCCCCACGAGAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTAAAACAGCAAAAAGGGGGAATTTAGTGTCGCAAGATAACAACt < 2:484694/287‑1 (MQ=255) aTCATGCCTGCGGACCTGAACCGTAGGTCGGATAGGGCGCTCTCGTCGAGTCGGACACCATGCTCAGATGCCTTATGCGACGCTGACGCATCTTATCAGGCCTTCCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGc < 2:27131/288‑1 (MQ=255) gCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGGTGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTcc > 1:98746/1‑289 (MQ=255) aaCCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAACCGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCgtatctggccgg > 2:459763/1‑278 (MQ=255) aaCCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGgcg < 1:121362/287‑1 (MQ=255) aaCCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGcgcg > 1:338061/1‑289 (MQ=255) tAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑g > 1:582190/1‑289 (MQ=255) tAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑g > 1:496891/1‑289 (MQ=255) ggccacgcgGCGCAACCGAAACCATGGCAAGATTCATGATGCAACGCTCACGCGTATTATCAGGCCTACCCACGTTTTTTAAACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATAAAAAAAAAATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCAtt < 2:126944/284‑1 (MQ=255) tCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTCCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCCGCGAGGAAA‑GGGGTATTCGCATTGAcc > 2:761639/1‑288 (MQ=255) cgcgTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCCCAGTCGGCGCGGAAC‑GGGGTCGTGGCATTGACgg > 2:526908/1‑288 (MQ=255) acacCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGGCGGCGCGGAAA‑GGGGGATTGGCCTTCACGTTCGTCATACTggg > 2:12141/1‑289 (MQ=255) aTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCttt > 1:756783/1‑289 (MQ=255) cGACACTGACGCGTCTTATCAGGCCTACCCACTATATTTACACCCATAATTCTTCCCCCACCATTTTGCATGCATTCATATAAAAAATATATTTCCCCACAAAAACGATTGCTTTTTATCTTCAGTTGAATAGATTGCGGCGGATTTTTTAGGTTTCAAACAGCAAAAAGGGGGAATTTCGTATCGCAAGATACCAACTTTAGCCAGGGGCCAGTCCCGCATTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGCGATTAACCTTCTTTTCCGCCAGTa < 2:514524/289‑1 (MQ=255) cGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTAtgt > 1:666246/1‑289 (MQ=255) aCGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCAAGTATGTGGAcc > 1:383137/1‑289 (MQ=255) aTCCGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGGAGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCggcggc < 1:140563/286‑1 (MQ=255) tACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGCGGCACTCTCGGAAc > 2:313629/1‑289 (MQ=255) cccACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGCGGCACTCTCGGAAc < 1:498354/287‑1 (MQ=255) cccACTGTTTTTACACCCGTAATTTTTCCCCCAACTTTTTGCCGTCATTCATATAAAAAATATATTTCCCCACGAAAACGCTTGCTTTTTATCCTCAGATGAGTAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACGTTAGCCAGCGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGCGGCACTCTCGGAAc < 2:613472/287‑1 (MQ=255) tGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACTTTCTTTTCCGCCAGTATGTGGACCGGCGGCACTCCCGGAACCGGTCt > 1:87158/1‑288 (MQ=255) tGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGCGGCACCCCCGGCACCGGTCtt > 2:557397/1‑289 (MQ=255) tttttACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGCACAGTGCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCCGCCTACAGGGATTAACCTTATTTTCCGCCAGTCTGTCGACCGGCGGCACCCTCGGACCGGGTCTTAg > 2:24807/1‑289 (MQ=255) cacCGATAATTTTTCCCCAACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCGGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGCGGCACTCTCGGAACCGGTCTTAGCTATCa < 2:315774/289‑1 (MQ=255) acCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGCGGCACTCTCGGAACCGGTCTTAGCTATCAt < 1:313629/289‑1 (MQ=255) ccGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGCGGCACTCTCGGAACCGGTCTTAGCTATCAt > 1:695914/1‑288 (MQ=255) ccGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAACGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGCAAA‑GGGGTATTGGCATTGACGTTCGTAATGCTGGGATTAACCTTCTTTTCGGCCAGATTGTGGACGGCCGGCACTCCCGGATCCGGTCTTAGcaaac > 1:175924/1‑282 (MQ=255) gATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATTCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGGGGCACTCACGGAACTGGCGTTAGCTAACATGAt > 1:494510/1‑289 (MQ=255) gATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGCGGCACTCTCGGaa < 2:570019/269‑1 (MQ=255) gATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGCGGCACTCTCGGaa > 1:570019/1‑269 (MQ=255) aTAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGCGGCACTCTCGGAACCGGTCTTAGCTATCATGAt < 2:338061/289‑1 (MQ=255) tttttCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGCGGCACTCTCGGAACCGGTCTTAGCTATCATGATttctt < 1:305355/289‑1 (MQ=255) ttttCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCGGCCAGTATGTGGACCGGCGGCACTCTCGGAACCGGTCTTAGCTATCATGATttctt < 1:290905/288‑1 (MQ=255) cacccACCTATTTTCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAACAAAAAGGGGGGATTTCGTGTCGCAAGATAACAACTTTCGCCAGTGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGCGGCACTCTCGGAACCGGTCTTAGCTATCATGATTTCTTCCTCg < 1:16040/287‑1 (MQ=255) ttttGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGCGGCACTCTCGGAACCGGTCTTAGCTACCATGATTTCTTCCTCGCAGTTCTCa > 1:186250/1‑289 (MQ=255) ttGCATTCATTCATATGAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGCGGCACTCTCGGAACCGGTCTTAGCTATCATGATTTCTTCCTCGCAGTTCTCAt < 2:695914/288‑1 (MQ=255) | CTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAAAAAGGGGGAATTTCGTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAA‑GGGGTATTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGCGGCACTCTCGGAACCGGTCTTAGCTATCATGATTTCTTCCTCGCAGTTCTCAT > NC_000913/354533‑355094 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |