New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4195525 =172 (1.690)4 (0.040) 4/376 NT 2.7% coding (575/1896 nt) thiC phosphomethylpyrimidine synthase
?NC_000913 4195682 = 122 (1.260)coding (418/1896 nt) thiC phosphomethylpyrimidine synthase
Rejected: Frequency below/above cutoff threshold.

TACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCAGATGATGGCCTCGACGAACTGCGTTTTAGCGGCGTACTAACACCAAAACGCGCCAAAGCAGGACGCCGTGTCACCCAACTGCACTACGCCCGCCAGGGCATCATCACGCCGGAAATGGAATTCATCGCCATCCGCGAGAATATGGGCCGCGAGCGCATCCGTAGCGAGGTTTTACGCCACCAGCATCCGGGAATGAGCTTTGGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4195770‑4195525
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gctttggcgcGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTG  >  NC_000913/4195682‑4195916
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
TACTGTGCGCAGTGCAGATTACACTAAAGGCTGGCTGGCCGACGATGCCCACGACGAACTGCGTTTTAGCGGCGTACTAACACCAAAACGCGCCAAAGCAGGACGCCGTGTCCCCCAACTGCACTCCGCCCGCCAGGGCATCATCACGCCGGAAATGGAATTCATCGCCATCCGCGAGAATATGGGCCGCGAGCGCATCCGTAGCGAGGTTTTACGCCACCAGCATCCGGGAATGAGCT                                                                                                                                                                                                                                                    <  2:381718/239‑1
 ACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCAGATGATGGCCTCGACGAACTGCGTTTTAGCGGCGTACTAACACCAAAACGCGCCAAAGCAGGACGCCGTGTCACCCAACTGCACTACGCCCGCCAGGGCATCATCACGCCGGAAATGGAATTCATCGCCATCCGCGAGAATATGGGCCGCGAGCGCATCCGTAGCGAGGGTTTACGCCACCAGCATCCGGGAATGAGCTT                                                                                                                                                                                                                                                   >  1:305102/1‑239
     TGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCAGATGATGGCCTCGACGAACTGCGTTTTAGCGGCGTACTAACACCAAAACGCGCCAAAGCAGGACGCCGTGTCACCCAACTGCACTACGCCCGCCAGGGCATCATCACGCCGGAAATGGAATTCATCGCCATCCGCGAGAATATGGGCCGCGAGCGCATCCGTAGCGAGGTTTTACGCCACCAGCATCCGGGAATGAGCTTTGGC                                                                                                                                                                                                                                               >  2:1019582/1‑239
      cacCAGTCCCGATTCCAATAAGGCGCGGCTGACAGATGAGGGCCTCGACGAACTTCTTTTTAGCGGCCTCCTACCCCCAAACCCCGCCAAAGCAGGCCGCCTGTTCCCCCACCTGCACTCCGCCCGCCAGGGCATCATCCCGCCGGAAATGGAATTCATCGCCATCCGCGAGAATATGGGCCGCGAGCGCATCCGTAGCGAGGTTTTACGCCACCAGCATCCGGGAATGAGCTTTGGCG                                                                                                                                                                                                                                              <  2:319778/236‑1
       CGCAGTTCCGATTACACTAAAGCGCGGCTGGCAGATGATGGCCTCGACGAACTGCGTTTTAGCGGCGTACTAACACCAAAACGCGCCAAAGCAGGACGCCGTGTCACCCAACTGCACTACGCCCGCCAGGGCATCATCACGCCGGAAATGGAATTCATCGCCATCCGCGAGAATATGGGCCGCGAGCGCATCCGTAGCGAGGTTTTACGCCACCAGCATCCGGGAATGAGCTTTGGCG                                                                                                                                                                                                                                              >  1:1136458/1‑238
                                                                                                                    AACTGCACTACGCCCGCCAGGGCATCATCACGCCGGAAATGGAATTCATCGCCATCCGCGAGAATATGGGCCGCGAGCGCATCCGTAGCGAGGTTTTACGCCACCAGCATCCGGGAATGAGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGT                                                                                                                                                                          <  1:135344/197‑1
                                                                                                                    AACTGCACTACGCCCGCCAGGGCATCATCACGCCGGAAATGGAATTCATCGCCATCCGCGAGAATATGGGCCGCGAGCGCATCCGTAGCGAGGTTTTACGCCACCAGCATCCGGGAATGAGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGT                                                                                                                                                                          >  2:135344/1‑197
                                                                                                                      CTGCACTACGCCCGCCAGGGCATCATCACGCCGGAAATGGAATTCATCGCCATCCGCGAGAATATGGGCCGCGAGCGCATCCGTAGCGAGGTTTTACGCCACCAGCATCCGGGAATGAGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGTGCTTTAGTGTAATCGGAAATGCGCACGGTAAGTTTTTCGGTATCGCCGCGt                                                                                                                               >  2:740765/1‑237
                                                                                                                                 CCGCCAGGGCATCATCACGCCGGAAATGGAATTCATCGCCATCCGCGAGAATATGGGCCGCGAGCGCATCCGTAGCGAGGTTTTACGCCACCAGCATCCGGGAATGAGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCAC                                                                                                                   >  1:556019/1‑239
                                                                                                                                                                                                                                            GCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTG        <  2:1059330/239‑1
                                                                                                                                                                                                                                             CTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTAC          <  1:604914/236‑1
                                                                                                                                                                                                                                               TTGGCGGGTTTGGGTTTTAGTCCGCCGCTAAAACGCAGTTCGGCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGG     <  2:1087132/239‑1
                                                                                                                                                                                                                                               TTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTCTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGG     <  1:688635/239‑1
                                                                                                                                                                                                                                                TGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGGC    <  1:440331/239‑1
                                                                                                                                                                                                                                                  GCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGG                                                                           >  2:1239510/1‑166
                                                                                                                                                                                                                                                  GCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGG                                                                           <  1:1239510/166‑1
                                                                                                                                                                                                                                                  GCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTCTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGCATTGCTTCGGTTTCTTCGTAatccg      >  2:688635/1‑230
                                                                                                                                                                                                                                                   CGCGTTTTGGTGTTAGTACGCCGATAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTG  <  1:259262/238‑1
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
TACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCAGATGATGGCCTCGACGAACTGCGTTTTAGCGGCGTACTAACACCAAAACGCGCCAAAGCAGGACGCCGTGTCACCCAACTGCACTACGCCCGCCAGGGCATCATCACGCCGGAAATGGAATTCATCGCCATCCGCGAGAATATGGGCCGCGAGCGCATCCGTAGCGAGGTTTTACGCCACCAGCATCCGGGAATGAGCTTTGGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4195770‑4195525
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gctttggcgcGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTAAGTTCTTCGGTATCGCCGCGCGCATCGATCCACGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTG  >  NC_000913/4195682‑4195916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.