Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,149,907 A→C 22.2% V360G (GTG→GGG)  yegI ← protein kinase‑related putative non‑specific DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,149,9070AC22.2% 104.3 / 8.3 71V360G (GTG→GGG) yegIprotein kinase‑related putative non‑specific DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (33/21);  new base C (3/14);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTACGCTTTGCTTGTCAGACTGCCAATAATCCAGATAGCCGCCAGCACAGGAACCAAAATAATATAACGCGGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCG  >  NC_000913/2149672‑2150125
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                             
gTACGCTTTGCTTGTCAGACTGCCAATAATCCAGATAGCCGCCAGCACAGGAACCAAAATAATATAACGCGGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCa                                                                                                                                                                                                                            <  1:61276/239‑1 (MQ=255)
                    tGCCAATAATCCAGATAGCCGCCAGCACAGGAACCAAAATAATATAACGCGGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTAACTACGCCCTCACCCTCGCCCCGACTTTTGCCAGc                                                                                                                                                                                                        >  1:1171042/1‑239 (MQ=255)
                                   tgccagcaaGCAAAGGAACAAAAAAAATAAACCGGGGTTCTGCGAAAAGGCCGAAAAGCAGAGGAGATAATGCTGACAGTGCGATCCCAGGAAGAATGTTGTATTCGCCCCGAACCAGCCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAAGGATCGGGTAATGGCAGTTGCAAGGCTGGCGGTGCTACTGACGCCATCCCCATCGCCCAGCCTTTCGCCAGCACAAAATTCccgccg                                                                                                                                                                                        <  2:930943/231‑1 (MQ=255)
                                     gccgccAGCACAGGAACCAAAATAATATAACGCGGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTTCCGCCggt                                                                                                                                                                                      >  1:497264/1‑239 (MQ=255)
                                      ccgccAGCACAGGAACCAAAATAATATAACGCGGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGGGCTACTGACGCCCTCACCATTGCCCAGACTTTCGCCAGCACCAAATTTCCGCCcgtg                                                                                                                                                                                     >  1:1087641/1‑239 (MQ=255)
                                       cgccAGCACAGGAACCAAAATAATATAACGCGGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCCGACTTTCGCCAGCACAAAAATTCCGCCcgtgg                                                                                                                                                                                    >  1:811368/1‑239 (MQ=255)
                                                  gAACCAAAATAATATAACGCGGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTTCAATGCTGGCGGGGGTACTGACGCCATCACCATCGCCCCGACTTTCGCCAGCACAAAATTTCCCCCCggtggaattggccct                                                                                                                                                                          >  2:335195/1‑229 (MQ=255)
                                                         aataatATAACGCGGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGca                                                                                                                                                                                                       <  1:739393/203‑1 (MQ=255)
                                                                  aCGCGGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGGAATGACCTCTTCGCCGAGATCAATaa                                                                                                                                                         >  1:879360/1‑239 (MQ=255)
                                                                   cgcgGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCCGACTTTTGCCAGCCCAAAATTCCCGCCGGTGGCAATGACCCCTTCGCCGGGAt                                                                                                                                                               >  1:97197/1‑232 (MQ=255)
                                                                   cgcgGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATTACCTCTTCGCCCGGATCCCTaaa                                                                                                                                                        >  1:1025767/1‑239 (MQ=255)
                                                                   cgcgGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCCATaaa                                                                                                                                                        >  1:1154919/1‑239 (MQ=255)
                                                                   cgcgGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCCCCCCGACTTTCGCCAGCCCCAAATTCCCCCCGGTGGTAATTACCTCTTCGCCGAGGTCCATaaa                                                                                                                                                        >  1:795753/1‑239 (MQ=255)
                                                                        ttCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAGTGGCAGTTGCAATGCTGGCGGTGGTACTGACGCCCACCACCACGCCCCGAATTTTGCCCGCGCAAAAATTCCGCCCGGGGGAATGAACCCTTTGCCCCGGTCAATtaaaaaag                                                                                                                                                   >  2:1182424/1‑235 (MQ=255)
                                                                        ttCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAAGGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTGGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCgg                                                                                                                                                                                       <  1:472702/203‑1 (MQ=255)
                                                                             ccTGAAGGCCGAAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGTTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCCCCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCgag                                                                                                                                                                 <  1:557903/220‑1 (MQ=255)
                                                                              cggAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAacac                                                                                                                                             <  1:335195/237‑1 (MQ=255)
                                                                                  aGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGGTACTGAAGCCATCACCATCGCCCAGAATTTCGCCCGCCCAAAAATCCCGCCGGGGGGAATGAACCCCTCGCCGAGGTCACTAAAATAGTGTACACCTTg                                                                                                                                         >  2:1078881/1‑239 (MQ=255)
                                                                                  aGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGGGCTACTGACGCCATCCCCATCGCCCAGACTTTTGCCCGCACAAAATTCCCGCCGGGGGGGATGAc                                                                                                                                                                             >  2:472702/1‑203 (MQ=255)
                                                                                  aGGCCGAAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGAGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGGGTTACTGACGCCATACCCATCGCCCAGACTTTTGCCCGGCCAAAATTTCCCCCCGGGGGAATGAGCCCCTTGCCGCGATCAATAAAAAAAATAacac                                                                                                                                             >  2:281534/1‑235 (MQ=255)
                                                                                       gCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGGGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTa                                                                                                                                     >  1:1027538/1‑238 (MQ=255)
                                                                                       gCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGGGGTACCGACGGCACTACCATCGCCCCAACTTTCGCCAGCACAAAATTTCCGCCGGTGGTAAAGACCTCTTTGCCCAGATCAATAACATAGCTAACAccccgagta                                                                                                                                     >  2:1077671/1‑231 (MQ=255)
                                                                                                   cGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCCTCGCCCAGACTTTTGCCAGCCCAAAATTCCCGCCGGGGGGAATGAACTCTTCGCCGAGGTCAATTAAATAGATAACACCCTGATTTTcccacgccaacc                                                                                                                        >  2:370021/1‑229 (MQ=255)
                                                                                                   cGATAATGCTGACAGTGCGATCCCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCgag                                                                                                                                                                 <  1:153978/198‑1 (MQ=255)
                                                                                                    gATAATGCTGACGGTGAGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGcaca                                                                                                                         <  1:136699/236‑1 (MQ=255)
                                                                                                    aaTAAGGCTGACGGTGCGATCTCAAGCAGAATGAGGTATTGGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCacac                                                                                                                        <  1:370021/236‑1 (MQ=255)
                                                                                                               cAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGGAATGACCTCTTCGCCGAGATCAATAAAAAAGATAAAACCTTGATATACCAGCGcaaa                                                                                                                         >  1:807110/1‑223 (MQ=255)
                                                                                                                    cGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTTACGCCATCCCCCTCGCCCAGACTTTCGCCCGCCCAAAATTCCCGCCGGGGGGAATGACCCCTTTGCCGAGGTCAATAAAATAGATAAAACCCTGGTTTTTCAAGGCACAACACCGGCAGTCCGTTaaa                                                                                                     >  2:1002374/1‑239 (MQ=255)
                                                                                                                       tctaAAGCAGAATGTTGTTTCCGCCCCGAAACGGGCCAAAAGGAGGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGCCGCCATCCCCATCGCCCAGACTTTCGCCAGCAAAAAATTCCCGCCGGTGGTAATGACctct                                                                                                                                                                         <  2:829961/170‑1 (MQ=255)
                                                                                                                        ctcAAGCAGAATGATGTATCCGGCCCGAAACAGGCCAAAAGGAACCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATCCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                                                 <  1:1078881/239‑1 (MQ=255)
                                                                                                                        ctcAAGCAGAAGGATGTATTCGCGCCGTAACGGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAGTGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                                                 <  1:1182424/239‑1 (MQ=255)
                                                                                                                                 aaTGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCCCCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGa                                                                                                                                                                              <  1:711589/155‑1 (MQ=255)
                                                                                                                                 aaTGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTTGCCAGCACAAAATTCCCGCCGGGGGTAATGACCCCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCAAACGCACACCACCGGCAGTCAGTTCAATTAGAGGGGGaaa                                                                                        >  2:167639/1‑239 (MQ=255)
                                                                                                                                 aaTGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGAt                                                                                                                                                               >  1:829961/1‑170 (MQ=255)
                                                                                                                                 aaTGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcag                                                                                                               <  1:902804/217‑1 (MQ=255)
                                                                                                                                 aaTGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAAAACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTaaa                                                                                        >  1:700091/1‑239 (MQ=255)
                                                                                                                                 aaTGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGGGCTACTGACGCCCTCACCATCGCCCAGACTTTTGCCAGCCCCAAATTCCCGCCGGGGGGAATGACCTCTTCGCCGAGATCAATAAAAAAGATAAAACCTTGGTTTTCCACCGGCAAACCACGGGAGTCAGTCCAAATGACAGGGTTaa                                                                                         >  2:768775/1‑239 (MQ=255)
                                                                                                                                           tCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGGGATCAATAAAATAGATAACACCTTGATTATCCAGCGCCAACCACGGGCAGTCAGTCCAATGAGCGGGGGAAACATGCAtt                                                                                >  1:1082334/1‑237 (MQ=255)
                                                                                                                                           tCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGGGGGAATGACCTCTTCGcc                                                                                                                                                                    >  2:711589/1‑155 (MQ=255)
                                                                                                                                           tCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACCGAAGCCATCACCAACGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGGGGGAATGACCCCTTCGCCCAGATCAATAAAATAGATAAAACCTTGATTATCCAGTGCACACCCCGGGCAGTCAGTCaaaa                                                                                                    >  2:902804/1‑216 (MQ=255)
                                                                                                                                             gcgcCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTTCCGCCCGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAAAACCTTGATTATCCAGCGCACACCACCGGCAGTCAGTCAAATAAGCGGGGTGAACAAGCaat                                                                                >  2:747639/1‑233 (MQ=255)
                                                                                                                                               gcCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCgag                                                                                                                                                                 <  1:1136492/154‑1 (MQ=255)
                                                                                                                                               gcCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGGGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAAATCCCGCCGGTGGGAATGACCTCTTCGCCGAGATCAATAAAAAAAGTAACACCTTGATTATCCAGCGCACACCACGGGCAGACAGTTAAATTAGCAGGGGAAAAATGCACTGCTGaa                                                                          >  2:423915/1‑239 (MQ=255)
                                                                                                                                                      cgtgCCTAAAGGAGGCGGCCTGCCAGTCGGTTGAAAATGAGCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCAAGCCTTTCGCCAGCAAAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATAc                                                                   <  2:700091/236‑1 (MQ=255)
                                                                                                                                                         gCCTAAGGGAAGGGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcag                                                                                                               <  1:626438/193‑1 (MQ=255)
                                                                                                                                                         gCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTTCTGACGCCATTACCATCGCCCAGACTTTTGCCAGCACAAAATTCCCGCCGGGGGTAATGACCCCCTTGTCGCGATCAAAAAAACAGATAAAACCTTGCATTTTCAGTGCCAACTACGGGCAGTCAGTCAAATGAGCTGGGGAAAAATGGATTGCTGAAACGACAAAtt                                                                >  2:135745/1‑239 (MQ=255)
                                                                                                                                                         gCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCCTCACCATCGCCCAGACTTTCGCCCGCACAAAATTCCCGCCGGGGGGAATGACCCCTTCGCCGGGATCAATaaaa                                                                                                                                                       >  2:1136492/1‑154 (MQ=255)
                                                                                                                                                             aaaGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCAAAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCtt                                                            <  1:60447/239‑1 (MQ=255)
                                                                                                                                                              aaGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCttt                                                           >  1:127121/1‑239 (MQ=255)
                                                                                                                                                                   cgCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCCCCATCGCCCAGACTTCCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                                                      <  1:135745/238‑1 (MQ=255)
                                                                                                                                                                   aggcgacTGCAAGTGGGTTGGAAATGGTCGGTTAGTGGCAGTGCCAATGCTGGCGGTGCTACTGCCGCCATCACACTCCCCCAGACTTTCGCCACCAAAAATTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                                                      <  2:1027538/233‑1 (MQ=255)
                                                                                                                                                                   aGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGGAATGAACCCTTCGCCCAGATCAATAAAAAAGAAAAAACCTTGATTATTCAGCGGCAACCACAGGCAGTCAGTCAAAt                                                                                                    >  2:626438/1‑193 (MQ=255)
                                                                                                                                                                   aGCGGCCGGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCCCCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                                                      <  1:747639/239‑1 (MQ=255)
                                                                                                                                                                    gCGGCCGGCCAGTGGGTTGGAAATGTCCGGGAAAGGGCAGTTGCAATGCTGGGGGTGCTACTGACGCCATCCCCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                                                      <  2:1025767/238‑1 (MQ=255)
                                                                                                                                                                    gCGACCGGCCAGTCGGTTGGAAAGGAGCGGGTAATGCCAGTTGCAATGCTGGCGGTGCTACGGACGCCATCACCATCGCCAAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                                                      <  2:795753/238‑1 (MQ=255)
                                                                                                                                                                    agggCCTGGCAAGTCGGTAGAAATTGATCGGTTAATGGCATTTCAAAGGCTGGCGTTGCACCTGCCGCCATCCCCATCGCCAAGACTTCCGCCAGCACAAATTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                                                      <  2:1171042/237‑1 (MQ=255)
                                                                                                                                                                      ggCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCCCCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGtt                                                    <  1:44657/238‑1 (MQ=255)
                                                                                                                                                                         cTGCCAGTCGGTTGAAAATGATCGGGTAAGGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCCCCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGc                                                 <  1:281534/238‑1 (MQ=255)
                                                                                                                                                                            gcatgTCGGTTGAAAATGCTGGGTTAACGCCATTGCAAATGCTGGCGGTCCACCGGCCCCCAACACCACCCCCCAGTCTTTCGCCAGCAAAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTa                                              <  2:1003435/235‑1 (MQ=255)
                                                                                                                                                                              cattgggTTGAAAATGATGGGGTAAGGGCAGTTGCAAGGCTGGCGGTGCTACTGACGCCATCCCCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTaga                                            <  1:1002374/234‑1 (MQ=255)
                                                                                                                                                                                 gcGGTTGGAAATGCTGGGGTATGGGCAGGTGCATGGCGGGCGGTGCTACTCACGCAATCCCCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTaga                                            <  2:811368/235‑1 (MQ=255)
                                                                                                                                                                                       ggAAAGGATCGGGTAATGGCAGTGGCAATGCTGGCGGTTCTACTGACGCAATCACCATCGCCAAGACTTCCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAg                                    <  1:227385/238‑1 (MQ=255)
                                                                                                                                                                                          aattgacGGGAAAGGGCAGTGGCAATGCTGGCGTGTCTACGGACGCCATCCCCATCGCCAAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGcc                                <  2:1154919/233‑1 (MQ=255)
                                                                                                                                                                                                cGGGTAATGGAAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCAAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTAcc                          <  1:1217188/239‑1 (MQ=255)
                                                                                                                                                                                                 gggTAATGGCATTTGCAATGCGTGGGGTGCTACTGACGCCATCCCCATCGCCCAGACTTTCGCCAGCACAAAATCCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTAcc                          <  1:1127141/238‑1 (MQ=255)
                                                                                                                                                                                                             ttGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTa                 >  1:1172280/1‑235 (MQ=255)
                                                                                                                                                                                                                        ggCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                                                 >  2:179960/1‑145 (MQ=255)
                                                                                                                                                                                                                        ggCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                                                 <  1:179960/145‑1 (MQ=255)
                                                                                                                                                                                                                         gCGGTGCTACTGACCCCATCACCATCGCCCCGACTTTCGCCAGCACAAAATTCCCGCCCGTGGTAATGACCTCTTCGCCGCGATCAATAAAATAGATAACACCTTGATTATCCCGCGCACACCAAGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACAGTTGGGGTAGAGAGGCCAGTGCCGCTACCCACGCTTTTGCAGTCTGATGCCCg  >  2:771881/1‑239 (MQ=255)
                                                                                                                                                                                                                                       gCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTg                                                   <  2:492845/176‑1 (MQ=255)
                                                                                                                                                                                                                                       gCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTg                                                   >  1:492845/1‑176 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                             
GTACGCTTTGCTTGTCAGACTGCCAATAATCCAGATAGCCGCCAGCACAGGAACCAAAATAATATAACGCGGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCG  >  NC_000913/2149672‑2150125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: