Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,828,510 G→T 10.7% M258I (ATG→ATT srlD → sorbitol‑6‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,828,5100GT10.7% 233.7 / 2.6 81M258I (ATG→ATTsrlDsorbitol‑6‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (32/39);  new base T (2/8);  total (34/47)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

GCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGG  >  NC_000913/2828273‑2828738
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                     
gCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATg                                                                                                                                                                                                                                       <  2:939494/238‑1 (MQ=255)
 cATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGtt                                                                                                                                                                                                                                     >  1:13332/1‑239 (MQ=255)
      aCTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGATGtt                                                                                                                                                                                                                                     >  1:1190676/1‑234 (MQ=255)
                ggTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCCAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCACGGGATGTTCTTATCAACaaccgg                                                                                                                                                                                                                      >  1:821684/1‑234 (MQ=255)
                ggTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGGTGGTCTGATCAACaaccgg                                                                                                                                                                                                                      >  2:265475/1‑234 (MQ=255)
                  tAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGgag                                                                                                                                                                                                                     <  1:541835/238‑1 (MQ=255)
                  tAACCTGCTGAAATCGCCGATGTGCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTg                                                                                                                                                                                                                                  <  1:475534/225‑1 (MQ=255)
                   aaCCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGgaga                                                                                                                                                                                                                    <  1:18982/238‑1 (MQ=255)
                    aCCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATc                                                                                                                                                                                                                  <  1:485939/239‑1 (MQ=255)
                            aaaTCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGATGGTCTGGTTAACAACCgaggacccctaagg                                                                                                                                                                                                          >  2:252641/1‑228 (MQ=255)
                            aaaTCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGCACAGTCGTTCAATGTCACCGGCTGGTAGGAGATGATTTGCTCTACAGCAgagaatcactctag                                                                                                                                                                                                          >  2:1176345/1‑229 (MQ=255)
                              aTCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGAt                                                                                                                                                                                                       <  2:1180945/239‑1 (MQ=255)
                                   cGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCg                                                                                                                                                                                                  <  2:13332/239‑1 (MQ=255)
                                   cGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCg                                                                                                                                                                                                  <  1:1156363/239‑1 (MQ=255)
                                   cGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCg                                                                                                                                                                                                  <  1:1176345/239‑1 (MQ=255)
                                       gTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTgag                                                                                                                                                                                              <  1:183732/239‑1 (MQ=255)
                                         tCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGAc                                                                                                                                                                                            <  1:252641/239‑1 (MQ=255)
                                                              aCGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTa                                                                                                                                                                       <  2:550425/239‑1 (MQ=255)
                                                                  gACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGgag                                                                                                                                                                                                                     <  1:132755/190‑1 (MQ=255)
                                                                  aaCCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACg                                                                                                                                                                     <  2:624392/236‑1 (MQ=255)
                                                                     cAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCtcat                                                                                                                                                                <  1:479749/239‑1 (MQ=255)
                                                                       aGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCACGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGTATTTTCTGGTTAACAACGGGGAACCCTTAAGGAGTTCCCTGGGACCATAGCATGCCTGATGGGCTACGCccatca                                                                                                                                                              >  1:988790/1‑239 (MQ=255)
                                                                       aGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCCGGGGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGGTGGACCATAGAATGCCTGATGCGCTaaccccatcc                                                                                                                                                              >  1:761247/1‑230 (MQ=255)
                                                                       aGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGATGTTTTGATTCAAAGCGGGGAGCCCTTAAGGGGATCCGTGAGGCTATAGTATGCTCGAGACGCTACCCtcatca                                                                                                                                                              >  2:850772/1‑239 (MQ=255)
                                                                          tGGGTAACAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTGAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAg                                                                                                                                                             <  2:1064237/237‑1 (MQ=255)
                                                                            ggTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCCGGTGGTGTTCTTATCAACCGCCGGGATCCCTTaaa                                                                                                                                                                                                          >  2:132755/1‑189 (MQ=255)
                                                                              tATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGAt                                                                                                                                                                                                       >  1:420627/1‑191 (MQ=255)
                                                                              tATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGAt                                                                                                                                                                                                       <  2:420627/191‑1 (MQ=255)
                                                                                 cAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAgg                                                                                                                                                    <  2:341550/239‑1 (MQ=255)
                                                                                            aaGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCtcatc                                                                                                                                                               <  1:551200/217‑1 (MQ=255)
                                                                                                cGAGCAGGATCACAGCGACAAAGCACCGCACAACCGCGGCTGCGATTAACAAGATGTGCAGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATAAATGTCACCGGCGGTCAGGTGATTTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACa                                                                                                                                     <  2:761247/239‑1 (MQ=255)
                                                                                                 gagaAGTATTACATCAACAAGGTCCCCTCAAACCGCGGCTGCGATAATCAAGTTGCGATGAATCTGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCCCCGGACAGTCGATAAATGTCCCCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACAt                                                                                                                                    <  2:1190676/239‑1 (MQ=255)
                                                                                                      agagggaacagACAATGTCCCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTAAATTGGCTGCTGTTCTCCCCCAGTCCTAAGGCGTCGTACTGCCCCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATTTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAgg                                                                                                                               <  2:1161384/229‑1 (MQ=255)
                                                                                                       tATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGgag                                                                                                                                                                                                                     <  1:515293/153‑1 (MQ=255)
                                                                                                       tATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTa                                                                                                                                                                       <  1:1092826/198‑1 (MQ=255)
                                                                                                        aTTACTGCGACAAAGTACCGCTCAACCGGGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGaa                                                                                                                             <  1:274212/239‑1 (MQ=255)
                                                                                                        aTTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCCCCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGaa                                                                                                                             <  1:265475/239‑1 (MQ=255)
                                                                                                          tACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCCCCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGaaaa                                                                                                                           <  1:628179/239‑1 (MQ=255)
                                                                                                            cATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGATGTTCTGATCAACAGCGGGGATCCATTAAGGGTCTCCGTGAGCATATAGAATGCCTGATGGGCTCCGCCCCTTAGGCACACAAGACTTCCGCCCCTACATTAAAGAAAAgg                                                                                                                         >  2:316427/1‑238 (MQ=255)
                                                                                                               cGAAAAGGTCCCGCTCAACCGCGCATGCAGTTATAAAGTGGTCTTGAATGTGCTACTTTCCCACGCAATCCATAGGGCGTCGTCCGCACCGGGAAGGTCGATAAATGTCAGCGGGGTTAAGGTGATTTTCTGATCAACAGCGGAGATCCATTAAGGATCTCGGGGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTAt                                                                                                                      <  2:815675/239‑1 (MQ=255)
                                                                                                                gACAAAGTCCCGCTCAAACGCGGCTGCGATTATCAAGATTTGCTGAATAGGCTTCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATg                                                                                                                     <  1:1048584/239‑1 (MQ=255)
                                                                                                                 aCAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGAt                                                                                                                                                                                                                                <  1:455318/132‑1 (MQ=255)
                                                                                                                 aCAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGAt                                                                                                                                                                                                                                >  2:455318/1‑132 (MQ=255)
                                                                                                                 aCAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCg                                                                                                                                                                                                  >  1:783778/1‑161 (MQ=255)
                                                                                                                 aCAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCg                                                                                                                                                                                                  <  2:783778/161‑1 (MQ=255)
                                                                                                                 aCAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGAc                                                                                                                                                                                            <  2:311945/167‑1 (MQ=255)
                                                                                                                 aCAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGAc                                                                                                                                                                                            >  1:311945/1‑167 (MQ=255)
                                                                                                                 aCAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCCGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATgg                                                                                                                    >  1:354725/1‑239 (MQ=255)
                                                                                                                 aCAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATgg                                                                                                                    >  1:707608/1‑239 (MQ=255)
                                                                                                                 aCAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGATGGTTTGATCAACAGCGGCGATCCATTAAGGATCTCCGTGGGACTATAGAATGCCCGATGCGCTACGCTCATCAg                                                                                                                                                             >  2:1092826/1‑198 (MQ=255)
                                                                                                                     aGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATc                                                                                                                                                                                                                  <  1:725769/142‑1 (MQ=255)
                                                                                                                     aGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGATGTTCTGATCAACAGCGGAGATc                                                                                                                                                                                                                  >  2:725769/1‑142 (MQ=255)
                                                                                                                       tACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAg                                                                                                                                                             <  1:65887/192‑1 (MQ=255)
                                                                                                                           gCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCCCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCAc                                                                                                           >  1:1060421/1‑238 (MQ=255)
                                                                                                                            cTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCCCATCAGGCATACAGGACTTCCGCCCCTACATTAAGGAAAAGTTATGGGATCCGCACtc                                                                                                         >  1:1150773/1‑239 (MQ=255)
                                                                                                                            cTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGATTTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTAtt                                                                                                                     >  1:1161384/1‑226 (MQ=255)
                                                                                                                            cTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGATGTTCTGATCAACAGCGGGGGTCCATTAAGGATCTCCGGGAGACTTTAGAATGCCTGATTGGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACtc                                                                                                         >  1:331128/1‑239 (MQ=255)
                                                                                                                                 aCGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGAt                                                                                                                                                                                                       >  1:954213/1‑140 (MQ=255)
                                                                                                                                 aCGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGAt                                                                                                                                                                                                       <  2:954213/140‑1 (MQ=255)
                                                                                                                                 aCGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGAACCATTAAGGATCTCCGTGGGACTATAGAATGCCCGATGCGCTAAGCTCATCAGGCATACAggg                                                                                                                                                   >  2:65887/1‑191 (MQ=255)
                                                                                                                                                    agttgtgATGAATATGCTGCTTTTCTACGCCAGCCCTAAGGCGTCGTACTGCACGGGACGGTCGATAAATGTCACCGGGGGTAGGGTGATTTTCTGATCAACGGCGGAGATCCATTAAGGATCTCGGTGAGCCTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTg                                                                                  <  2:1051985/236‑1 (MQ=255)
                                                                                                                                                                        tgTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGGTTGCGCACCTGGCCTTAg                                                              >  1:327897/1‑239 (MQ=255)
                                                                                                                                                                          ttCTACGCCAGTCCTAAGGGGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGGGGAGACCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATcc                                                                                                               <  1:956704/188‑1 (MQ=255)
                                                                                                                                                                            cTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGATGTTCTGATCCACAGCGGAGATCCATTAAGGGTCTCCGTGGGACTATAGAATGCCTGATTCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGGTATGGTATCCGCACTCATCACCGTCGCCCGTATCGCCTGGTGTGCGCCACAGGCCtttagcg                                                           >  2:942814/1‑233 (MQ=255)
                                                                                                                                                                                    gTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCCGGTGGTGTTCTGATCAACAGCGGGGATCCCTTAAGGATCTCCGTGGGACTATAGAAAGCCTGATGCGCTACGCTCATCAGGGATACATGACTTCCGCCCCCACATTAAGGAAAAATTATTGTAACCGccaccatta                                                                                                     >  2:956704/1‑180 (MQ=255)
                                                                                                                                                                                     tCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTAt                                                                                                                      <  1:1019261/170‑1 (MQ=255)
                                                                                                                                                                                               tcgtACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGGTATCGCCTGGTGTGGGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGtttt                                       >  1:756397/1‑239 (MQ=255)
                                                                                                                                                                                                     tcccccGGACATTCGATCAATGTCACCGGCGGTCAGGTTATGTTCTGATCAACGGCGGAGATCCTTTAAGGATCTCCGTGAGCCTATAAAATGCCTGATGCCCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCg                                                                                            <  2:966410/176‑1 (MQ=255)
                                                                                                                                                                                                     tGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATTTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGt                                 <  1:862189/239‑1 (MQ=255)
                                                                                                                                                                                                     tGCACAGGACGTTCGATAACTGTCACGGGCGGTCAGGTGATGTTCTGATCAAGAGCGGAGATCAATTAAGGATCTCCGTGAGACTAAAAAATGCCTGATGCGCTACCCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGt                                 <  2:756397/239‑1 (MQ=255)
                                                                                                                                                                                                      gcaacagaaccgTCGATCAATGTCCCCGGCGTTCAGGTGATTTTCTGATCAACAGCGGAGATCCATTAAGGATCTCGGTGAGCCTATAGAATGCCTGATGCGCTACGCTCATCGGGCATACAGGCCTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGt                                 <  2:354725/228‑1 (MQ=255)
                                                                                                                                                                                                      gCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGATGTTCTGATCAACAGCGGAGATCCATTTAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCCCATCAGGCATACAGGGCTTTCCCCCCTACATTAAGGGAAAGTTATGGTATCCGCACTCCTCACCCTCCCCGTTTTCCCCTTGGGTGGGCAACTGGGCCTAGGCGGCTGGGCAAATTCTCGGTTTTATCGt                                 >  2:862189/1‑238 (MQ=255)
                                                                                                                                                                                                       ccccGGACAGTCGTTCAATGTCCCCGGCGTTCAGGTATTTTTCTAACCAACGGCGGACACCCATTAAGGATCCCCGTGAGACTATAGAATCCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAAttt                                               <  2:734121/221‑1 (MQ=255)
                                                                                                                                                                                                        cccGGAAAGTCGATCAATGCCCCCGGCGGTAAGGTGATTTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGc                               <  1:316427/237‑1 (MQ=255)
                                                                                                                                                                                                          cGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGGTTTTACCGTGCCt                             >  1:973157/1‑238 (MQ=255)
                                                                                                                                                                                                                aGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTg                                                                                  >  1:966410/1‑180 (MQ=255)
                                                                                                                                                                                                                  tCGATCAATGTCACCGGCGGTCAGGGGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGGGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTaa                                     >  1:734121/1‑223 (MQ=255)
                                                                                                                                                                                                                   cGATCAATGTCACCGGGGGTCAGGTGATGTTCTGATCAACAGCGGAGTTCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGa                         <  1:742969/234‑1 (MQ=255)
                                                                                                                                                                                                                     aTCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGCCTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGtt                                                                                          <  2:605261/167‑1 (MQ=255)
                                                                                                                                                                                                                     aTCAATGTCACCGGCGGTCAGGGGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGtt                                                                                          >  1:605261/1‑167 (MQ=255)
                                                                                                                                                                                                                                cggcgtTCAGGATATGTTCATAACAACGGGGAACTCCAATAAAGGCTCTCGGGGAAACTAAAAAATCCCTGATGCCCTACGCTCATCGGGCAAACAGGCCTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAgg       <  2:331128/239‑1 (MQ=255)
                                                                                                                                                                                                                                      taagttGATGTTCGGATCACCAGCGGAGATCCATTAAGGATCTCCGGGAGACTATAGAATGCCTGATGCGCAACGCTCATCAGGCATACAGGCCTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCggg  <  1:850772/233‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                     
GCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATTACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGG  >  NC_000913/2828273‑2828738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: