Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0310CT23.7% 80.0 / 14.5 38intergenic (‑94/+109)ves/spycold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base C (24/5);  new base T (0/9);  total (24/14)
Fisher's exact test for biased strand distribution p-value = 1.23e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCC  >  NC_000913/1824806‑1825266
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           
acgtgtggatgCAATGCTGGCACGCCAGTAAAAATCACGTTTGTCCGGTGGGAACGTGCACATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAAc                                                                                                                                                                                                                                 <  2:537596/233‑1 (MQ=255)
                                        tttGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAcc                                                                                                                                                                                         >  1:187654/1‑238 (MQ=255)
                                             cGGTAGGAACGTAAAAGTTGCGCGCATTTCACCGCAAGCTTCCCCCCCAATACTCCCCTACTTTTTCCGAATAACAAGTTTTTCCATCCTCGCGCCCGCCCTTTTTTTTTCATTTTTATCGTTCAGGTACCCGT‑GGATGTTTTCCGGCAACT‑GGGGTGATTTACGTTAGTGTTGATCGGTTTTTTTTTACACTTTTTTGAGAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTa                                                                                                                                                                                   <  2:572754/239‑1 (MQ=255)
                                                      cGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTgagaa                                                                                                                                                                                                                       <  1:18432/194‑1 (MQ=255)
                                                      cGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTgagaa                                                                                                                                                                                                                       >  2:18432/1‑194 (MQ=255)
                                                                                                                     atCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTcagca                                                                                                                       >  1:846170/1‑227 (MQ=255)
                                                                                                                               tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTTGCTGTAAGATTATTCTACAAGTTCAGGCACTTTACCattt                                                                                                  >  2:109776/1‑238 (MQ=255)
                                                                                                                                     cTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTgagaaga                                                                                                                                                                                                                     <  1:296579/117‑1 (MQ=255)
                                                                                                                                     cTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTgagaaga                                                                                                                                                                                                                     <  1:593552/117‑1 (MQ=255)
                                                                                                                                     cTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTgagaaga                                                                                                                                                                                                                     >  2:593552/1‑117 (MQ=255)
                                                                                                                                     cTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTgagaaga                                                                                                                                                                                                                     >  2:296579/1‑117 (MQ=255)
                                                                                                                                      tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGa                                                                                                                                                               >  1:443285/1‑170 (MQ=255)
                                                                                                                                         cTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACg                                                                                       >  1:403510/1‑239 (MQ=255)
                                                                                                                                          tCCCGGCCCTTTTTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGTTTATCGGCAACT‑GGGGTGATTTACGTTAGTGGTGATCGGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTc                                                                                                                                                                                             <  1:618517/136‑1 (MQ=255)
                                                                                                                                                   gTTTTTTTCATTGTTATCGTTGA‑GTAACCGTGGGTTGGTTA‑CCGCAAGTGAGGGTTATTTACGTAGGGGGTACTAGGGTTTTTTTAACAATTTTTTAAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGa                                                                             <  2:403510/239‑1 (MQ=255)
                                                                                                                                                    ttCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTa                                                                                                                                                                                   >  2:618517/1‑136 (MQ=255)
                                                                                                                                                                        cAGGTAACCGT‑GGATGGTTATCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAc                                                                                                                                                                                                                                    >  1:562864/1‑67 (MQ=255)
                                                                                                                                                                        cAGGTAACCGT‑GGATGGTTATCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAc                                                                                                                                                                                                                                    <  2:562864/67‑1 (MQ=255)
                                                                                                                                                                        cAGGTAACCGT‑GGATGGTTATCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCCGTTGCCGGCATTTTTCCTTTTTCCGCTGGCCGTTCTTTCAGACGCTTCTCACAATTatactta                                                        >  1:74785/1‑233 (MQ=255)
                                                                                                                                                                              aCCGT‑GGATGGTTATCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAAtt                                                    >  1:554043/1‑237 (MQ=255)
                                                                                                                                                                                     gtggtttctCGACAACT‑GGGGTAATTAACTTTAGTGGTGATCAGGTTTTTTTAACAACTTTTTGAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAg                                                                                                                <  2:413712/163‑1 (MQ=255)
                                                                                                                                                                                          ttATCGGCACCT‑GGGGTGTTTTACGTTAGTGTTGATCAGGTTTTTTTTACAACTTTTTGAAAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg                                                                                                                                                           <  2:266351/123‑1 (MQ=255)
                                                                                                                                                                                             tCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTTAATTTCCTTTTCTGTTCCg                                     >  1:382656/1‑238 (MQ=255)
                                                                                                                                                                                             agcggaatctggggTTATTTACGTTGGTGGTGATCAGTTTTTTTTAACAATTGTTGGAGAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCg                                     <  1:856575/228‑1 (MQ=255)
                                                                                                                                                                                               ggCATCT‑GGGGTGTTTTCCTTTAGTGGTAATCAGGTTTTTTTTACAACTGTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCa                                                           <  1:130949/214‑1 (MQ=255)
                                                                                                                                                                                               ggCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAcc                                                                                                      >  1:413712/1‑171 (MQ=255)
                                                                                                                                                                                                gCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGTAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTTGCTGGAAGATTATTCAGCAGGTGCAGGCAATTTAACTTTTTCCCCCGGAAGTTTTGTCAAACGGTTTCCAAAATTTGCATTTAATTTCTTTTTCTGtttcctgg                                  >  2:856575/1‑232 (MQ=255)
                                                                                                                                                                                                    cT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAg                                                                                                                                                 >  1:266351/1‑123 (MQ=255)
                                                                                                                                                                                                    cT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAgg                             >  1:152853/1‑239 (MQ=255)
                                                                                                                                                                                                          gTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTTGCAGACGATTCTCCAAATTAGCATTAAATTGca                                                 >  2:130949/1‑213 (MQ=255)
                                                                                                                                                                                                              tttACGTTGGGGGTGATCGGGTTTTTTTTACAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAt                                                                                                                                                                                     <  1:695812/78‑1 (MQ=255)
                                                                                                                                                                                                                tACGTTAGTGGTGATCAGGTTTTTTTAACAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttt                                                                                                                                             <  2:925161/116‑1 (MQ=255)
                                                                                                                                                                                                                        tggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAgg                                                                                                                                                                           >  2:695812/1‑78 (MQ=255)
                                                                                                                                                                                                                          gtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaaa                                                                                                                                   >  1:925161/1‑116 (MQ=255)
                                                                                                                                                                                                                             aTCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcg                                                                                                                                                         >  1:655534/1‑91 (MQ=255)
                                                                                                                                                                                                                             aTCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt                                                                                     >  1:552087/1‑159 (MQ=255)
                                                                                                                                                                                                                                  gCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTTTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTGTTCCGGCCTTAGGATGTTGTCAATTTTGTTCTGGGTTTcc  >  1:923832/1‑237 (MQ=255)
                                                                                                                                                                                                                                  gCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCCTTAGGTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAGTTTAACTTTTTCCGCTgg                                                                                          >  2:515438/1‑149 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           
CGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACT‑CGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCC  >  NC_000913/1824806‑1825266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑