Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,386,8620GA50.0% ‑3.8 / 16.7 16intergenic (+31/‑72)yfbL/yfbMputative M28A family peptidase/DUF1877 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/8);  new base A (8/0);  total (8/8)
Fisher's exact test for biased strand distribution p-value = 1.55e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCG‑AAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGA  >  NC_000913/2386640‑2387050
                                                                                                                                                                                                                               |                                                                                                                                                                                             
aTTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTCTAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTTCCGACAAAACGACATgtgcacattt                                                                                                                                                                               >  1:846165/1‑230 (MQ=255)
                  tctGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGTCCGGAAAAAAGAAAATGACAattttatttttctccatca                                                                                                                                                                  >  2:431584/1‑226 (MQ=255)
                                     ggCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGCCAGAAAAAACGACCAGGACCATTTATTTTTCCTCCTCTGTTTTTAg                                                                                                                                                          >  1:608698/1‑223 (MQ=255)
                                                                          cGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTGCCGCAAAAAAGACACGCACAATTTTTTTTTTCCCTTCTGTTTTTATATGCGCAAAAACTAAATTATACGATGGAAAATAAAAAAGGAATGTTTgacatt                                                                                                     >  2:588824/1‑234 (MQ=255)
                                                                           gCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCG‑AAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTAttt                                                                                                   <  1:142103/239‑1 (MQ=255)
                                                                                           aaCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCAGAAAAAGCGACATGCACattt                                                                                                                                                                                 <  1:906317/146‑1 (MQ=255)
                                                                                                     cTTGCCCGGTGGTACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGTCCGGAAAAAAC‑AAAAGCACatttttttttttct                                                                                                                                                                       >  2:906317/1‑142 (MQ=255)
                                                                                                                                                                 tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCG‑AAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGa                                                                                 <  1:590307/171‑1 (MQ=255)
                                                                                                                                                                            tGTTATACAACAGTAAATAATAAAAATTTCTCGAGTC‑GGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCG‑AAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGa  <  2:846165/239‑1 (MQ=255)
                                                                                                                                                                            tGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCG‑AAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGa  <  1:397878/239‑1 (MQ=255)
                                                                                                                                                                              ttATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCG‑AAACATGAAATTCAACggagg                                                                                                                               <  2:310953/112‑1 (MQ=255)
                                                                                                                                                                                        gTAAATAATAAAAATTTCTCGAGGC‑GGGGCCCGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTTTTTTTAGATGCG‑GAAAATGAAAATTAAAGGAGGAAaaataaat                                                                                                                     >  1:310953/1‑112 (MQ=255)
                                                                                                                                                                                                taaAAATTTCTCGAGGC‑GGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCAt                                                                                                                                                                    <  2:47007/58‑1 (MQ=255)
                                                                                                                                                                                                taaAAATTTCTCGAGGC‑GGGTCCAGAAAAAACGACATGCACATTTTATTTTTCCTCAt                                                                                                                                                                    >  1:47007/1‑58 (MQ=255)
                                                                                                                                                                                                    aaTTTCTCGAGGC‑GGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCG‑AAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAAcc                                                                      <  1:56068/147‑1 (MQ=255)
                                                                                                                                                                                                              ggC‑GGGTCCAGAAAAAAAGAAAAGCACATTTTATTTTTCCTCATCTGTTTTTAGATGCG‑AAAAAAGAAAATCAAAAGAGGAAAAATAAATGGGAATGATTGGGTATTTTTTAGAAAATGAATCAGAAAAAAATAAACAAAAGCTTGa                                                            >  2:56068/1‑147 (MQ=255)
                                                                                                                                                                                                                               |                                                                                                                                                                                             
ATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCG‑AAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGA  >  NC_000913/2386640‑2387050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑