Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,101,514 | 0 | G | T | 26.8% | 74.7 / 14.1 | 41 | intergenic (+84/‑176) | sodA/kdgT | superoxide dismutase, Mn/2‑keto‑3‑deoxy‑D‑gluconate transporter |
Reads supporting (aligned to +/- strand): ref base G (7/23); new base T (11/0); total (18/23) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.01e-05 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
TTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGTTTCAATTGATG‑TGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAA > NC_000913/4101276‑4101716 | tttCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTtg < 1:589416/239‑1 (MQ=255) cccGGTTTCACGGTTTGGGGCCTGGATGTGTGGAAACATGCTTCCTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTc < 2:746022/238‑1 (MQ=255) tGGATGTGTGGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGat < 1:738350/239‑1 (MQ=255) gATGTGTGGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GGTTTTTTGTGATCAATTTTCAA‑ATAAAAAAAATG‑ATCCCatta > 1:666535/1‑237 (MQ=255) tgGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGataaaaataa > 1:333834/1‑239 (MQ=255) aaCATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAAc < 1:915317/238‑1 (MQ=255) tGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑GTGTTTTTTTTTG‑TTAACTTCAAATATAAAAAAAATG‑ATACGATCAATATATAAAAGct > 2:124278/1‑238 (MQ=255) ctacCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGTTTCAAt > 1:876672/1‑239 (MQ=255) aaaTTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGTTTCAATTGAtg‑tg < 2:104759/239‑1 (MQ=255) gAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGTTTCAATTGATG‑TGGTTTTGAt < 2:333834/239‑1 (MQ=255) tCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAAttt < 1:325117/170‑1 (MQ=255) ggAATACGTTAAAGAGTTCGTGAACGTGGTCAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTGTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGTTTCAATTGATG‑TGGTTTTGATCACTTTTATTGa < 2:91938/239‑1 (MQ=255) aTTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGTTTCAATTGATG‑TGGTTTTGATCACTTTTATTGATTAaatg < 1:487277/239‑1 (MQ=255) aaaGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGTTTCAATTGATG‑TGGTTTTGATCACTTTTATTGATTAaatgaat < 1:571615/239‑1 (MQ=255) aaGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GGTTTTTTTTGTTCAATTTTTAA‑ATAAAAAAAAAA‑ATCCGCTAAAAATAAAAAACAGGTTTAATTTATG‑TTGGTTTTATCACCTTTTTTGTTtaaataaaaa > 2:278074/1‑237 (MQ=255) ttCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GGGTTTTTTTTGTAAACTTTAAA‑ATAAAAAAAAAGTATCGGTACAAAAAAAAAAAACGTTTCATTGTATG‑GGTTTTTGTTAACTTTTTTAGTTTaattaaa > 2:568656/1‑228 (MQ=255) aCGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGTTTCAATTGATG‑TGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGtt < 1:447883/239‑1 (MQ=255) tggtgAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTc < 1:135964/142‑1 (MQ=255) gtgAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGTTTCAATTGATG‑TGGTTTTGATCACTTTTATTGATTAaatgaat < 1:491984/221‑1 (MQ=255) gAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGTTTCAATTGATG‑TGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATc < 1:278074/238‑1 (MQ=255) aCTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GGTTTTTTTTGATCAATTTCAAA‑ATAAAAACAAAG‑ATCCCATAAAAATAAAACAACGTTTCAATTGATG‑TGGTTTTGTTCACCTTTATTGATTAAATGAAAGTCTATCTTTCTTTTCATCAac > 1:4200/1‑239 (MQ=255) ggACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GGTTTTTTTTGATCAAATTCTAa‑aaaaaaa > 2:135964/1‑142 (MQ=255) aCGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGG‑GTTTTTTTTTGTTAAATTTC‑AA‑ATAAAAAAAAAT‑ATTCCATAAAAATAAAAAAAAGGTTTCATTGTTGTTGGTTTTG‑TCACTTTTTTTGATTTAAATAATGttcatttttg > 2:491984/1‑213 (MQ=255) cGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGTTTCAATTGATG‑TGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATg < 2:876672/239‑1 (MQ=255) cGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGTTTCAATTGATG‑TGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTcc < 1:70141/213‑1 (MQ=255) cgAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GGTTTTTTTTGGTCAATTTTAAA‑AAAAAAAAAAAG‑AATCGGTTAAAAAAAAAAAACGCTTTAATTTATG‑TGGGTTTTTTTCCTTTTTTTTATTTAATAAATATCTATATTCGTTTTCATCAACACTg > 2:70141/1‑213 (MQ=255) cgccTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGTTTCAATTGATG‑TGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGa < 2:149945/239‑1 (MQ=255) gccTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGTTTCAATTGATG‑TGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGAc < 1:596715/239‑1 (MQ=255) taaTGAGGCGTATAGGCCGGATATCAGCTTAAAAAATGAAACATCGCCAACGGCGGT‑G‑GTTTTGTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGataa < 1:108833/101‑1 (MQ=255) gCGTCTAGGCCCCATATCAGCTTAAAAAACGAACCAACGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGTTTCAATTGATG‑TGGTTTTGATCCCTTTTATTGATTAAATGAATGTCTATCTTCGtt < 2:429075/165‑1 (MQ=255) gCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑At < 2:57035/88‑1 (MQ=255) gCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑At > 1:57035/1‑88 (MQ=255) cGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGTTTCAATTGATG‑TGGTTTTGATCACTTTTATTGATTAaatgaa < 1:610623/150‑1 (MQ=255) cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTTTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAAAAGCGGTTCAATTGATG‑TGGGTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAaca > 1:429075/1‑165 (MQ=255) cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GGTTTTTTTTGATCAATTTCAAA‑AAAAAAAAAAAA‑ATCCGATAAAAAAAAAACAGAGTTTTAATTTATG‑TGGTTTTGATAACTtttttt > 2:16192/1‑130 (MQ=255) gCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GGTTTTTTTTGATCAATTTTAAA‑ATAAAAAAAAAG‑ATTCGATAAAAATAAAAAAGCGTTTTAATTGATG‑TGGGTTTTATTCCTTTTTTTTATTAAATGAATTTCTATCtt > 2:610623/1‑150 (MQ=255) cATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GGTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGTTTCAATTGATG‑TGGGTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAAAACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGc > 1:528337/1‑238 (MQ=255) ttAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGTTTCAATTGAtg < 1:51903/99‑1 (MQ=255) aaaaaaTGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTTTAA‑ATAAAAACAAAG‑ATCCGCTAAAAATAAAAAAGCGTTTTAATTGTTG‑TGGGTTTTATCACTTTTTTTGATTAAATGAATGTCTATTTTTGTTTCCATCAAAACTTATGCTCCATTTAGGAATTATGCATCAGCGCTTCTAAAAATGCCGACAGGGTATTGGAATGGAGATAAAACGACCGATTGAGaaa > 2:372370/1‑239 (MQ=255) aaCCATCGCCAACGGCGGT‑G‑GGTTTTTTTTGATCAAATTTAAA‑AAAAAAAAAAAG‑ATCCGATAAAAAAAAAAAAGCGTTTTAATTTATG‑TGGGTTTTAt > 2:51903/1‑99 (MQ=255) cggT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGt > 1:667948/1‑63 (MQ=255) cggT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGt < 2:667948/63‑1 (MQ=255) | TTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGT‑G‑GTTTTTTTGTGATCAATTTCAAA‑ATAAAAACAATG‑ATCCGATAAAAATAAAACAGCGTTTCAATTGATG‑TGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACGCATCAGCCCTTAAAAATATGCCGACAGGTGATGGAAATGCAGATAAAACGCTCGATTGAGAAA > NC_000913/4101276‑4101716 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |