Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,330,404 A→C pseudogene (3999/4605 nt) yfaS ← pseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,330,4040AC98.6% 263.3 / NA 70pseudogene (3999/4605 nt)yfaSpseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (33/36);  minor base T (0/1);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTATGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAGCAGCACAACCGTGTGCGCCAGCGGCTGATTGCTGTTTGCCGCTTTATCCCACGCATATT  >  NC_000913/2330189‑2330627
                                                                                                                                                                                                                          |                                                                                                                                                                                                                               
aCCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCcgcag                                                                                                                                                                                                           >  2:835815/1‑238 (MQ=255)
  ctgTTTCAGAAAACAGCTCTTCTCAGCTGCACGAGAGATAGGCCGATACAACTGGCCTGCAACGGTCCCCTGAATGTGTCTTTGTTGATCCATTTTTGCCGGCGCACGTCAGGGGACCTGCACATTGTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAGCGCCAGTCATCGCCCCCTCCAGTTAACATCTGTTTAGCCCAAGCGCCcgcaggcg                                                                                                                                                                                                        <  2:883877/237‑1 (MQ=255)
  cGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCcgcaggcg                                                                                                                                                                                                        <  2:651363/239‑1 (MQ=255)
                     aTCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGCTTAGCCCATGCGCCCGCAGGCGCAGGCCACACAAATGGaag                                                                                                                                                                                     >  2:752258/1‑237 (MQ=255)
                       ctctTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCa                                                                                                                                                                                   >  1:226938/1‑239 (MQ=255)
                       ctctTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCa                                                                                                                                                                                   >  1:60262/1‑239 (MQ=255)
                          ttcttcACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTg                                                                                                                                                                                <  1:835815/239‑1 (MQ=255)
                                aCCAGGGATAAGCCGATTCCACTGGCGTTCAACGGTCCCCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCa                                                                                                                                                                          <  2:60262/239‑1 (MQ=255)
                                               aTACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCgccagcc                                                                                                                                                           <  2:226938/239‑1 (MQ=255)
                                                               aCGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAgcgc                                                                                                                                           <  1:592602/239‑1 (MQ=255)
                                                                  gTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCCCCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGCCCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCCTGCGCCCGCATGCGCAGGCAAACCACCTGGAGGCATTGTCGCCGTATCTTTTGCCAGCCAGTTCCTGGCCAtcgctc                                                                                                                                         >  2:551955/1‑236 (MQ=255)
                                                                     aCCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTc                                                                                                                                     >  1:552079/1‑239 (MQ=255)
                                                                     aCCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTc                                                                                                                                     >  1:667212/1‑239 (MQ=255)
                                                                         gAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATa                                                                                                                                 <  1:180736/239‑1 (MQ=255)
                                                                                 cTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTg                                                                                                                                                                                >  2:628816/1‑184 (MQ=255)
                                                                                 cTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTg                                                                                                                                                                                <  1:628816/184‑1 (MQ=255)
                                                                                 cTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAgcgcgcg                                                                                                                                        <  2:750840/224‑1 (MQ=255)
                                                                                 cTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAgcgcgcg                                                                                                                                        >  1:750840/1‑224 (MQ=255)
                                                                                    tgttgAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTc                                                                                                                      >  1:829022/1‑239 (MQ=255)
                                                                                           ccATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTc                                                                                                               >  1:211364/1‑239 (MQ=255)
                                                                                              tttttGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCaaa                                                                                                            <  1:693802/239‑1 (MQ=255)
                                                                                                        cTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGTGCGCGCTCAATAGTGGATTGCTCAGCGGTCaaa                                                                                                            >  2:102448/1‑229 (MQ=255)
                                                                                                        cTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGTGCGCGCTCAATAGTGGATTGCTCAGCGGTCaaa                                                                                                            <  1:102448/229‑1 (MQ=255)
                                                                                                             gTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCg                                                                                             <  1:232081/239‑1 (MQ=255)
                                                                                                                  cGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCgccagc                                                                                                                                                            >  2:201544/1‑171 (MQ=255)
                                                                                                                  cGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCgccagc                                                                                                                                                            <  1:201544/171‑1 (MQ=255)
                                                                                                                   ggACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGc                                                                                           <  1:752258/235‑1 (MQ=255)
                                                                                                                   ggACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGc                                                                                           <  1:174152/235‑1 (MQ=255)
                                                                                                                     aCCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCcgcaggc                                                                                                                                                                                                         <  1:519261/123‑1 (MQ=255)
                                                                                                                     aCCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCcgcaggc                                                                                                                                                                                                         >  2:519261/1‑123 (MQ=255)
                                                                                                                       cTGCACATTTTGCGGCGATAATTCGTCACCAAAAGAGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTTGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGGGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCg                                                                                         >  1:559354/1‑235 (MQ=255)
                                                                                                                       cTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCaaa                                                                                                            <  2:401007/214‑1 (MQ=255)
                                                                                                                       cTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCaaa                                                                                                            >  1:401007/1‑214 (MQ=255)
                                                                                                                                    ggcgATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCAc                                                                       <  2:667212/238‑1 (MQ=255)
                                                                                                                                    ggcgAGAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCGTATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGGCTGGGTAGCGTCGCCAc                                                                       <  1:551955/238‑1 (MQ=255)
                                                                                                                                    ggccATAATTCGGACCCAAA‑‑AGGGCAAATGCCCGGCCAGCCCGGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCAc                                                                       <  2:211364/238‑1 (MQ=255)
                                                                                                                                                tCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCgccagcc                                                                                                                                                           <  1:244942/142‑1 (MQ=255)
                                                                                                                                                tCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCgccagcc                                                                                                                                                           >  2:244942/1‑142 (MQ=255)
                                                                                                                                                  gccAAA‑‑AGAGACAAGGTCCGGCACGCCCTGACCACCCCAACGCCATTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAgcagca                                                        <  2:407586/238‑1 (MQ=255)
                                                                                                                                                       a‑‑aGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAag                                                             >  1:871738/1‑229 (MQ=255)
                                                                                                                                                       a‑‑aGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAACGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAag                                                             <  2:871738/229‑1 (MQ=255)
                                                                                                                                                                aaTGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAgcgcgcg                                                                                                                                        <  2:722096/147‑1 (MQ=255)
                                                                                                                                                                aaTGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAgcgcgcg                                                                                                                                        >  1:722096/1‑147 (MQ=255)
                                                                                                                                                                aaTGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATATCGGCCGTCTGGGTAGCGTCGCCACCGCAATTAAGCAGATCAACCGTGTgc                                             >  2:926893/1‑238 (MQ=255)
                                                                                                                                                                    tCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGa                                                                                                                            >  1:205446/1‑155 (MQ=255)
                                                                                                                                                                    tCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGa                                                                                                                            <  2:205446/155‑1 (MQ=255)
                                                                                                                                                                      cGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAg                                                                                                                                                                                      <  1:555479/95‑1 (MQ=255)
                                                                                                                                                                      cGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAg                                                                                                                                                                                      >  2:555479/1‑95 (MQ=255)
                                                                                                                                                                                 gACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAgcgcgcg                                                                                                                                        >  2:215959/1‑130 (MQ=255)
                                                                                                                                                                                 gACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAgcgcgcg                                                                                                                                        <  1:215959/130‑1 (MQ=255)
                                                                                                                                                                                     aacccaacGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAGCAGCACAACCGTGTGCGCCAGCGGCTGATTGCTGTTTg                       <  1:926893/239‑1 (MQ=255)
                                                                                                                                                                                     aacccaacGCCAGCCCTCGCCCCCTCCAGTTAACTTTTGTTTACCCAATGCGCCCGAAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAGCAGCACAACCGTGTGCGCCAGCGGCTGATTGCTGTTTg                       <  2:281352/239‑1 (MQ=255)
                                                                                                                                                                                       cccaacGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCt                                                                                                                                      <  2:506118/126‑1 (MQ=255)
                                                                                                                                                                                       cccaacGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCt                                                                                                                                      >  1:506118/1‑126 (MQ=255)
                                                                                                                                                                                       cccaacGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGt                                                                                        >  2:436552/1‑172 (MQ=255)
                                                                                                                                                                                       cccaacGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGt                                                                                        <  1:436552/172‑1 (MQ=255)
                                                                                                                                                                                            cGCCAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAGCAGCACAACCGTGTGCGCCAGCGGCTGATTGCTGATTGCCGCttt                >  1:182437/1‑239 (MQ=255)
                                                                                                                                                                                              ccAGTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAGCAGCACAACCGTGTGCGCCAGCGGCTGATTGCTGTTTGCCGCTTTAt              <  2:829022/239‑1 (MQ=255)
                                                                                                                                                                                                 gTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTa                                                                  >  2:230914/1‑184 (MQ=255)
                                                                                                                                                                                                 gTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTa                                                                  <  1:230914/184‑1 (MQ=255)
                                                                                                                                                                                                 gTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTa                                                               <  2:215189/187‑1 (MQ=255)
                                                                                                                                                                                                 gTCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTa                                                               >  1:215189/1‑187 (MQ=255)
                                                                                                                                                                                                  tCTTCGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATATCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAGCAGCACAACCGTGTGCGCCAGCGGCTGATTGCTGTTTGCCGCTTTATctc           >  2:448652/1‑236 (MQ=255)
                                                                                                                                                                                                      cGCCCCCTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAACAGCCAAACCTGGTGCGCCAGCGGCTGATTGCTGTTTTCTGCTTTATCCCACGCa      >  2:409003/1‑239 (MQ=255)
                                                                                                                                                                                                       ccccccTTCAGATTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAGCAGCACAACCGTGTGCGCCAGCGGCTGATTGCTGTTTGCCGCTTTATCCCACGCa      <  2:182437/238‑1 (MQ=255)
                                                                                                                                                                                                            cTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCa                                                                                                                     >  2:350864/1‑122 (MQ=255)
                                                                                                                                                                                                            cTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCa                                                                                                                     <  1:350864/122‑1 (MQ=255)
                                                                                                                                                                                                            cTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCg                                                                                             <  2:292353/146‑1 (MQ=255)
                                                                                                                                                                                                            cTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCg                                                                                             >  1:292353/1‑146 (MQ=255)
                                                                                                                                                                                                            cTCCAGTTAACTTCTGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGGAGCGTCGCCACCGCTATTAAGCAGCACAACCGTGGGCGCCCGCGGCCGATTGCTGGTTGCCGCTTTATCCCACGccactt  >  2:74875/1‑232 (MQ=255)
                                                                                                                                                                                                                          |                                                                                                                                                                                                                               
ACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAA‑‑AGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTATGTTTAGCCCATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTGGATTGCTCAGCGGTCAAACCACTTAAAATAGCGGCCGTCTGGGTAGCGTCGCCACCGCTATTAAGCAGCACAACCGTGTGCGCCAGCGGCTGATTGCTGTTTGCCGCTTTATCCCACGCATATT  >  NC_000913/2330189‑2330627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: