BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	443,022	0	G	A	20.4%	369.6 / 90.7	181	intergenic (+25/+29)	thiI/yajL	tRNA s(4)U8 sulfurtransferase/oxidative‑stress‑resistance chaperone
Reads supporting (aligned to +/- strand): ref base G (66/78); new base A (25/12); total (91/90)
Fisher's exact test for biased strand distribution p-value = 2.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 191 total aligned reads displayed.

GGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCC  >  NC_000913/442923‑443121

ggggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTgg                                                                                                     >  1:3001433/1‑100 (MQ=255)

ggggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTgg                                                                                                     >  1:2725640/1‑100 (MQ=255)

  ggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTc                                                                                                   <  2:4435209/100‑1 (MQ=255)

   gTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCa                                                                                                  <  1:376369/100‑1 (MQ=255)

   gTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTGTTTACGTCGCATCTGGTCa                                                                                                  <  1:3895919/100‑1 (MQ=255)

   gTGATGAGCCGTCTGCACGAGCTCTATCTGCACGGGCAGGGCTTTAACAACGGGAAGGGATATCGCTCGTAATTTGGGGTTTTTACGCCGCATCTGGTCa                                                                                                  <  2:4214027/100‑1 (MQ=255)

       tgaGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATg                                                                                              <  2:2689648/100‑1 (MQ=255)

         aGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCg                                                                                            >  2:637710/1‑100 (MQ=255)

          gCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGa                                                                                           >  2:3417551/1‑100 (MQ=255)

          gCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGACCAGATGCGa                                                                                           <  2:828438/100‑1 (MQ=255)

           ccGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGACCAGATGCGAc                                                                                          <  1:4384096/100‑1 (MQ=255)

            cGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGACCAGATGCGACg                                                                                         <  1:931850/100‑1 (MQ=255)

             gTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGt                                                                                        >  1:2420326/1‑100 (MQ=255)

             gTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGACCAGATGCGACGt                                                                                        <  2:3597135/100‑1 (MQ=255)

             gTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTCAACAATGTGAAGGTATATGGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGt                                                                                        >  1:4244054/1‑100 (MQ=255)

              tCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGtt                                                                                       <  2:1626006/100‑1 (MQ=255)

                tGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTg                                                                                     <  2:2290645/100‑1 (MQ=255)

                 gCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGc                                                                                    >  2:2928720/1‑100 (MQ=255)

                  cAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGcc                                                                                   >  1:2026748/1‑100 (MQ=255)

                   aGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCg                                                                                  <  1:1821954/100‑1 (MQ=255)

                     gcgcTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCa                                                                                <  2:3818491/100‑1 (MQ=255)

                      cgcTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACTTCTCATCTGGTCAGATGCGACGTTTGCCGCAt                                                                               >  2:675078/1‑100 (MQ=255)

                       gcTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATc                                                                              <  1:1813813/100‑1 (MQ=255)

                        ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATcc                                                                             >  2:2357963/1‑100 (MQ=255)

                        ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATcc                                                                             <  2:3887645/100‑1 (MQ=255)

                        ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACATTTGCCGCATcc                                                                             <  2:3047881/100‑1 (MQ=255)

                         tctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTGCGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCg                                                                            >  2:4083342/1‑100 (MQ=255)

                         tctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCg                                                                            >  1:3318298/1‑100 (MQ=255)

                          ctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGa                                                                           <  1:1448639/100‑1 (MQ=255)

                           tATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGac                                                                          <  2:1984481/100‑1 (MQ=255)

                            aTCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGaca                                                                         >  1:4125577/1‑100 (MQ=255)

                             tCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGacac                                                                        <  2:2882918/100‑1 (MQ=255)

                             tCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGacac                                                                        <  2:2586284/100‑1 (MQ=255)

                               tGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACacta                                                                      >  1:2168046/1‑100 (MQ=255)

                                   cgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCg                                                                  <  1:1904642/100‑1 (MQ=255)

                                    gAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGt                                                                 <  1:986469/100‑1 (MQ=255)

                                    gAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGt                                                                 <  2:1605788/100‑1 (MQ=255)

                                    gAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGt                                                                 <  2:502194/100‑1 (MQ=255)

                                       cAGGGCTTTCACAATGTGAAGGTATATCGCCCGAAAATTGGGGTATTTACGTCGCATTTGGCCAGTTGCGCCGTTTGCCGCCTCCGACACTACTAGtaat                                                              >  2:4356004/1‑100 (MQ=255)

                                       cAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaat                                                              >  2:106318/1‑100 (MQ=255)

                                       cAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaat                                                              >  1:1792810/1‑100 (MQ=255)

                                        aGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaata                                                             <  1:1663303/100‑1 (MQ=255)

                                         gggCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaataa                                                            >  1:3238652/1‑100 (MQ=255)

                                          ggCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTaataat                                                           <  1:4216761/100‑1 (MQ=255)

                                          ggCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTaataat                                                           <  1:2671059/100‑1 (MQ=255)

                                           gCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAAtt                                                          >  2:1857244/1‑100 (MQ=255)

                                           gCTGTAACAATGTTAAGGTATATCGCCTTTACTTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAAtt                                                          <  2:3509098/100‑1 (MQ=255)

                                            cTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATta                                                         <  2:2300531/100‑1 (MQ=255)

                                              ttAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATtatn                                                       <  1:1835142/100‑2 (MQ=255)

                                                 aCAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAAt                                                    <  1:1146809/100‑1 (MQ=255)

                                                  cAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATc                                                   <  1:1204256/100‑1 (MQ=255)

                                                      gtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTg                                               >  2:1259180/1‑100 (MQ=255)

                                                       tgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgc                                              >  1:2472463/1‑100 (MQ=255)

                                                        gAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgcc                                             <  1:3496285/100‑1 (MQ=255)

                                                        gAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgcc                                             >  2:2102773/1‑100 (MQ=255)

                                                              atatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATc                                       >  1:1261331/1‑100 (MQ=255)

                                                                atCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAc                                     <  1:1387972/100‑1 (MQ=255)

                                                                 tCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAcc                                    <  2:3935495/100‑1 (MQ=255)

                                                                   gCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAg                                  >  2:3275311/1‑100 (MQ=255)

                                                                   gCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAg                                  >  1:397148/1‑100 (MQ=255)

                                                                    cccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGt                                 <  2:2492856/100‑1 (MQ=255)

                                                                     ccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGtt                                >  2:3129429/1‑100 (MQ=255)

                                                                        tAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTtgtg                             <  2:2641920/100‑1 (MQ=255)

                                                                         aaTTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa                            <  2:1342721/100‑1 (MQ=255)

                                                                          aTTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGAt                           <  2:2519100/100‑1 (MQ=255)

                                                                           tttGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATg                          <  2:1418818/100‑1 (MQ=255)

                                                                            ttGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGc                         <  1:4258350/100‑1 (MQ=255)

                                                                             tgtgGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGcc                        <  1:4336843/100‑1 (MQ=255)

                                                                              gtgGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCa                       >  1:2236826/1‑100 (MQ=255)

                                                                              gtgGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACCACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCa                       >  1:4492498/1‑100 (MQ=255)

                                                                               tgGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCAc                      <  2:3205632/100‑1 (MQ=255)

                                                                                ggTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACt                     >  1:1634937/1‑100 (MQ=255)

                                                                                   ttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCCTCACCAGTTGTGATGCCACTTCa                  >  1:1569718/1‑100 (MQ=255)

                                                                                    tttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCAt                 >  1:4405153/1‑100 (MQ=255)

                                                                                    caaaCGTCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCAt                 >  1:2006711/4‑100 (MQ=39)

                                                                                     ttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATg                >  1:3483724/1‑100 (MQ=255)

                                                                                      aaCGTCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATgg               >  2:1115988/2‑100 (MQ=255)

                                                                                       aCGTCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATggg              >  2:1097119/1‑100 (MQ=255)

                                                                                        cGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGc             >  2:3364584/1‑100 (MQ=255)

                                                                                        cGTCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGc             >  1:3701979/1‑100 (MQ=255)

                                                                                        cGTCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGc             >  2:4292260/1‑100 (MQ=255)

                                                                                        cGTCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGc             >  1:2078749/1‑100 (MQ=255)

                                                                                        cGTCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGc             >  1:3409908/1‑100 (MQ=255)

                                                                                         gTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCt            >  1:106688/1‑100 (MQ=255)

                                                                                         gTCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTCCTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCt            >  2:3778988/1‑100 (MQ=255)

                                                                                         gTCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCt            >  1:1727097/1‑100 (MQ=255)

                                                                                         gTCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCt            >  1:2848565/1‑100 (MQ=255)

                                                                                         gTCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCt            >  1:2591441/1‑100 (MQ=255)

                                                                                          tCGCATCTGACCAGATGCGACGTTTGCCGCATTCGACAATACTCGTCATAATTATAAATCCCTGCCGCCATCACCAGTTGCGATGCCACTTCATGGGCtt           >  1:4057385/1‑100 (MQ=255)

                                                                                          tCGCATCTGACCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCtt           >  1:883262/1‑100 (MQ=255)

                                                                                           cGCATGTGGTCAGATGCGACGTTTGCCGCATCCGACACTACGCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCttt          <  2:1754705/100‑1 (MQ=255)

                                                                                            gCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAAATATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCtttt         <  2:2368960/100‑1 (MQ=255)

                                                                                                cTGGTCAGATGCGACGTTTGCCGCGTCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCAGCACCAGTTGTGATGCCACTTCATGGGCTTTTTCAc     <  2:1512047/100‑1 (MQ=255)

                                                                                                cTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCAc     <  1:541513/100‑1 (MQ=255)

                                                                                                 tGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACg    <  1:2569891/100‑1 (MQ=255)

                                                                                                  ggTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGc   >  1:3834791/1‑100 (MQ=255)

                                                                                                  ggTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGc   >  2:2739785/1‑100 (MQ=255)

                                                                                                  ggTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGc   >  2:1529515/1‑100 (MQ=255)

                                                                                                   gTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGcc  >  2:2807868/1‑100 (MQ=255)

                                                                                                   gTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGcc  <  1:4241766/100‑1 (MQ=255)

GGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCC  >  NC_000913/442923‑443121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`