Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,089,222 G→A D212D (GAC→GAT pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,089,2220GA98.0% 504.8 / ‑1.5 155D212D (GAC→GATpgaBpoly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (72/79);  total (74/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 155 total aligned reads displayed.

GATTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGGTCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGC  >  NC_000913/1089125‑1089318
                                                                                                 |                                                                                                
gATTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATc                                                                                                <  1:1128643/100‑1 (MQ=255)
gATTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATc                                                                                                <  2:1839014/100‑1 (MQ=255)
gATTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATc                                                                                                <  1:4438634/100‑1 (MQ=255)
gATTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATc                                                                                                <  1:1988223/100‑1 (MQ=255)
 aTTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCa                                                                                               <  2:1727432/100‑1 (MQ=255)
 aTTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCa                                                                                               <  1:4302351/100‑1 (MQ=255)
    tACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTa                                                                                            >  2:703082/1‑100 (MQ=255)
    tACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTa                                                                                            >  2:4039451/1‑100 (MQ=255)
    tACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTa                                                                                            >  1:1828314/1‑100 (MQ=255)
     agcTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGATTTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTaa                                                                                           <  2:4468210/98‑1 (MQ=255)
      ccTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGAATTCTGCTGCGCTTTGATACCGTGCGTGATCAGTaaa                                                                                          >  1:4256116/1‑100 (MQ=255)
         cAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAata                                                                                       <  2:4111428/100‑1 (MQ=255)
          aaCCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAatat                                                                                      >  2:1429018/1‑100 (MQ=255)
          aaCCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAatat                                                                                      >  1:3812827/1‑100 (MQ=255)
          aaCCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAatat                                                                                      >  1:2635403/1‑100 (MQ=255)
           aCCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATg                                                                                     <  2:1315228/100‑1 (MQ=255)
            ccTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGc                                                                                    >  2:2003929/1‑100 (MQ=255)
             cTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCa                                                                                   >  1:3406670/1‑100 (MQ=255)
              tttGTACGCGGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCAc                                                                                  <  2:2579376/100‑1 (MQ=255)
               ttGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACg                                                                                 <  2:17901/100‑1 (MQ=255)
               ttGTACGCAGGTATTCCGTCAGTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACg                                                                                 <  1:4450352/100‑1 (MQ=255)
                 gTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGAt                                                                               >  2:3710462/1‑100 (MQ=255)
                   aCGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGAttt                                                                             <  2:1740807/100‑1 (MQ=255)
                    cGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTa                                                                            <  1:4118443/100‑1 (MQ=255)
                      cAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACa                                                                          >  1:907382/1‑100 (MQ=255)
                      cAGGTATTCCGTCATTTTTACAGCAACCAGACGAATTCTTTCCCGGTATTCTTCTGCGGTTTCATACCATGCGTGATCAGTAAAATATGCACGATTTACa                                                                          >  2:4311972/1‑100 (MQ=255)
                        ggTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACata                                                                        <  2:105631/100‑1 (MQ=255)
                        ggTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCAGGTATTCTGCTGCGGTTTCATACCGTGCGTGCTCAGTAAAATATGCCCGATTTACatc                                                                        >  1:4568143/1‑99 (MQ=255)
                             tCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAg                                                                   >  1:3027838/1‑100 (MQ=255)
                              ccGTCATTTTTACAGCTTCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTCTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAgg                                                                  >  2:1356599/1‑100 (MQ=255)
                               cGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGc                                                                 <  1:2327243/100‑1 (MQ=255)
                               cGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGc                                                                 <  2:3452843/100‑1 (MQ=255)
                               cGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGc                                                                 >  1:2623946/1‑100 (MQ=255)
                                gTCATTTTTATATCTTCTATACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGGGCGTGATCAGTAAAATATGCACGTTTTACATATACAGGCa                                                                >  2:2355518/1‑100 (MQ=255)
                                gTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCTGTAAAATATGCACGATTCACATATACAGGCa                                                                >  2:3520939/1‑100 (MQ=255)
                                gTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCa                                                                >  2:260122/1‑100 (MQ=255)
                                gTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCa                                                                <  2:58541/100‑1 (MQ=255)
                                 tCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCaa                                                               <  1:2046942/100‑1 (MQ=255)
                                  cATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAAt                                                              <  1:3067775/100‑1 (MQ=255)
                                   aTTTTTACAGCATCCAGACGAATTCCTTCCCGGTATTCTGTTGCGGTTTCATACCGGCCGTGATCAGTAAAATATGCCCGATTTACATATCCAGGCGATa                                                             >  2:3481695/1‑100 (MQ=255)
                                     ttttACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAg                                                           <  1:1920526/100‑1 (MQ=255)
                                     ttttACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAg                                                           >  2:4216704/1‑100 (MQ=255)
                                        tACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTg                                                        >  1:2482878/1‑100 (MQ=255)
                                         aCAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGc                                                       >  2:738299/1‑100 (MQ=255)
                                         aCAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGc                                                       >  2:2528599/1‑100 (MQ=255)
                                          cAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGcc                                                      <  1:4350169/100‑1 (MQ=255)
                                            gCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCgg                                                    >  2:3557832/1‑100 (MQ=255)
                                            gCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCgg                                                    <  2:2311219/100‑1 (MQ=255)
                                             cATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGt                                                   <  1:848888/100‑1 (MQ=255)
                                              aTCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTg                                                  >  2:4371291/1‑100 (MQ=255)
                                              aTCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTg                                                  >  2:4373624/1‑100 (MQ=255)
                                                ccAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGc                                                <  2:1601358/100‑1 (MQ=255)
                                                    aCGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTTCCGGTGGCATTa                                            >  2:2453522/1‑100 (MQ=255)
                                                    aCGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTa                                            <  2:2270866/100‑1 (MQ=255)
                                                    aCGAATTCTTGCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTa                                            <  1:2048422/100‑1 (MQ=255)
                                                     cGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAg                                           >  1:996303/1‑100 (MQ=255)
                                                     cGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAg                                           <  1:3080653/100‑1 (MQ=255)
                                                     cGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATACACAGGTAATAAGCTGCCGGTGGCATTAg                                           >  1:4161659/1‑100 (MQ=255)
                                                     cGAATTCTTTCCCGGTATTCTGCTGCGGGTTCATACCGTGCGTGAACAGTAAAATATGCACGAATTCCATATACAGGCAATCAGCTGCCGGTGGCATTAg                                           >  2:4013660/1‑100 (MQ=255)
                                                       aaTTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGcc                                         >  1:1257820/1‑100 (MQ=255)
                                                       aaTTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGcc                                         <  2:4317225/100‑1 (MQ=255)
                                                        aTTCTTTCCCGGTCTTCTGGTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCt                                        >  2:1427697/1‑100 (MQ=255)
                                                        aTTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCt                                        >  1:3474758/1‑100 (MQ=255)
                                                        aTTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCt                                        <  2:2706736/100‑1 (MQ=255)
                                                         ttCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTg                                       <  2:469744/100‑1 (MQ=255)
                                                          tCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGa                                      <  2:3044623/100‑1 (MQ=255)
                                                           cTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGaa                                     >  2:217960/1‑100 (MQ=255)
                                                            tttCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAAt                                    <  1:2926795/100‑1 (MQ=255)
                                                              tCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGCGGCATTAGCCTGAATAc                                  >  2:3034421/1‑100 (MQ=255)
                                                               cccGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATAcc                                 <  2:1761206/100‑1 (MQ=255)
                                                                ccGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCg                                >  2:3281019/1‑100 (MQ=255)
                                                                ccGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCg                                <  2:2374846/100‑1 (MQ=255)
                                                                   gTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAg                             <  1:4526061/100‑1 (MQ=255)
                                                                     aTTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTg                           <  2:575379/100‑1 (MQ=255)
                                                                       tCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTgag                         <  1:1159836/100‑1 (MQ=255)
                                                                       tCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTgag                         <  1:1773901/100‑1 (MQ=255)
                                                                        ctgctgCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTgaga                        >  1:22260/1‑100 (MQ=255)
                                                                         tgctgcGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGaa                       >  1:1687911/1‑100 (MQ=255)
                                                                          gctgcGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAAt                      >  1:2883920/1‑100 (MQ=255)
                                                                            tgcGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTc                    >  1:3466735/1‑100 (MQ=255)
                                                                             ncGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTcc                   >  1:3613244/2‑100 (MQ=255)
                                                                              cGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCa                  >  1:605533/1‑100 (MQ=255)
                                                                               ggTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCAt                 <  2:3364478/100‑1 (MQ=255)
                                                                               ggTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCAt                 <  1:3194388/100‑1 (MQ=255)
                                                                                gTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATg                <  1:3121281/100‑1 (MQ=255)
                                                                                gTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATg                >  1:3249270/1‑100 (MQ=255)
                                                                                gTTTCATAACGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATg                >  1:129904/1‑100 (MQ=255)
                                                                                 tttCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGt               <  2:4319509/100‑1 (MQ=255)
                                                                                    cATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATg            <  2:1583519/100‑1 (MQ=255)
                                                                                    cATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATg            <  1:1230770/100‑1 (MQ=255)
                                                                                     aTACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATga           >  1:2778803/1‑100 (MQ=255)
                                                                                      tACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATgag          >  1:2739836/1‑100 (MQ=255)
                                                                                      tACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGCCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATgag          <  2:3449266/100‑1 (MQ=255)
                                                                                         cgtgcgtgATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAg       <  2:891705/100‑1 (MQ=255)
                                                                                          gtgcgtgATCAGTAAAATATGCACGATTTACATATGCAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGc      <  2:3785998/100‑1 (MQ=255)
                                                                                           tgcgtgATCAGTAAAACATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCg     <  2:1359066/100‑1 (MQ=255)
                                                                                            gcgtgATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGa    <  1:754156/100‑1 (MQ=255)
                                                                                             cgtgATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAg   >  1:2555819/1‑100 (MQ=255)
                                                                                             cgtgATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAg   >  1:1396052/1‑100 (MQ=255)
                                                                                              gtgATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGc  >  1:3290063/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GATTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGGTCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGC  >  NC_000913/1089125‑1089318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: