BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,986,652	T→G	V269G (GTC→GGC)	yqeG →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,986,652	0	T	G	96.9%	547.5 / 2.9	162	V269G (GTC→GGC)	yqeG	putative transporter
Reads supporting (aligned to +/- strand): ref base T (3/2); new base G (81/74); total (84/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

CTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCAT  >  NC_000913/2986556‑2986747

cTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCAc                                                                                              <  1:3387463/100‑1 (MQ=255)

 ttATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCAcc                                                                                             <  2:1741637/100‑1 (MQ=255)

  tATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCa                                                                                            >  1:3376682/1‑100 (MQ=255)

  tATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCa                                                                                            >  2:626595/1‑100 (MQ=255)

   aTCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATCGAAGCTGCTAAAGAAGAAGGGGGCACCAt                                                                                           >  1:4169256/1‑100 (MQ=255)

     ctcATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCAttt                                                                                         >  1:4237445/1‑100 (MQ=255)

      tcatcaTCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCAtttt                                                                                        >  1:2356768/1‑100 (MQ=255)

       catcatCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTa                                                                                       <  1:674702/100‑1 (MQ=255)

          catcTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCg                                                                                    <  2:3365279/100‑1 (MQ=255)

           atcTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCgg                                                                                   >  2:224293/1‑100 (MQ=255)

             cTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGgcg                                                                                 >  1:1927337/1‑100 (MQ=255)

             cTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGgcg                                                                                 <  1:1665501/100‑1 (MQ=255)

             cTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGgcg                                                                                 <  1:3067684/100‑1 (MQ=255)

              tGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGgcgc                                                                                >  1:792838/1‑100 (MQ=255)

              tGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGgcgc                                                                                >  1:551640/1‑100 (MQ=255)

               gCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCt                                                                               >  1:795194/1‑100 (MQ=255)

               gCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCt                                                                               >  2:3287304/1‑100 (MQ=255)

                  tAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTc                                                                            <  1:2624278/100‑1 (MQ=255)

                  tAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTc                                                                            <  2:2908365/100‑1 (MQ=255)

                    aGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTa                                                                          >  2:1211976/1‑100 (MQ=255)

                    aGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTa                                                                          >  1:170017/1‑100 (MQ=255)

                       gtACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTAtgc                                                                       >  2:4212546/1‑100 (MQ=255)

                          cTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTAtgctgc                                                                    <  1:2155515/100‑1 (MQ=255)

                            gTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCg                                                                  >  2:2495172/1‑100 (MQ=255)

                            gTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCg                                                                  >  2:805602/1‑100 (MQ=255)

                              tCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATCGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGaa                                                                >  2:2069462/1‑100 (MQ=255)

                               cTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAAc                                                               <  2:1466632/100‑1 (MQ=255)

                               cTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAAc                                                               >  2:1747284/1‑100 (MQ=255)

                                tttGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACg                                                              <  2:1880313/100‑1 (MQ=255)

                                  tGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGcn                                                            <  1:3641978/100‑2 (MQ=255)

                                  tGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGcc                                                            <  1:3783162/100‑1 (MQ=255)

                                   gTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGccc                                                           <  1:3538793/100‑1 (MQ=255)

                                   gTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGccc                                                           >  2:1682082/1‑100 (MQ=255)

                                   gTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGccc                                                           <  1:2359740/100‑1 (MQ=255)

                                    tCTTTAGCTGTCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGcccc                                                          >  2:2716389/1‑100 (MQ=255)

                                    tCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGcccc                                                          <  2:2972442/100‑1 (MQ=255)

                                     cTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATTGGCGCTTTCTATGCTGCCGAACGCCCCa                                                         >  2:3656910/1‑100 (MQ=255)

                                        tAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCa                                                      >  1:2124369/1‑100 (MQ=255)

                                        tAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCa                                                      <  1:4481543/100‑1 (MQ=255)

                                        tAGCTGCCTGCTTTCTATTCCACCGTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCa                                                      <  1:4481413/100‑1 (MQ=255)

                                           ctgcctgcTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATgg                                                   <  1:1043624/100‑1 (MQ=255)

                                           ctgcctgcTTTCTATTCCACCTGCGTATATTGAAGCTGGTAAAGAAGAAGGGGGCACCATTTTATCGGGGCTGTCTGTGCTGCGGAACGCGCCAGCATgg                                                   >  2:3936458/1‑100 (MQ=255)

                                            tgcctgcTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGt                                                  >  1:3011073/1‑100 (MQ=255)

                                            tgcctgcTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGt                                                  <  2:3824490/100‑1 (MQ=255)

                                              cctgcTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTg                                                <  1:1894409/100‑1 (MQ=255)

                                               ctgcTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGt                                               >  2:1877431/1‑100 (MQ=255)

                                                tgcTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTc                                              <  2:4147011/100‑1 (MQ=255)

                                                  cTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCaa                                            >  2:2848982/1‑100 (MQ=255)

                                                  cTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCaa                                            >  1:4215859/1‑100 (MQ=255)

                                                    ttCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAAtt                                          >  1:2703256/1‑100 (MQ=255)

                                                     tCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAAttt                                         >  1:1792663/1‑100 (MQ=255)

                                                       tATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTcc                                       >  1:1949437/1‑100 (MQ=255)

                                                         ttCCACCTTCGTATATTGAAGCTGCTGAAGAAGAAGGGGTCACCACTTTATCGGCGCTTTCTATGCTGCCCAACGTCCCAGCATGGTTGTCAATTTCCgg                                     <  1:4267608/100‑1 (MQ=255)

                                                          tCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCggg                                    <  2:4361230/100‑1 (MQ=255)

                                                          tCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCggg                                    <  1:3321954/100‑1 (MQ=255)

                                                           ccACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGa                                   >  1:1642071/1‑100 (MQ=255)

                                                            cACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGat                                  <  2:4402743/100‑1 (MQ=255)

                                                            cACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGat                                  <  1:1272490/100‑1 (MQ=255)

                                                              ccTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGatta                                >  2:453138/1‑100 (MQ=255)

                                                              ccTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGatta                                <  1:1482653/100‑1 (MQ=255)

                                                              ccTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGatta                                >  2:3145246/1‑100 (MQ=255)

                                                              ccTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGatta                                >  1:620284/1‑100 (MQ=255)

                                                              ccTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGatta                                >  1:601864/1‑100 (MQ=255)

                                                              ccTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGatta                                >  2:4277441/1‑100 (MQ=255)

                                                                   gTATATTGAACCTGCTAAACAAGAAGGAGCAACCATTTTAGCTGCCCCTTGTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCCGGATTATTGTc                           <  2:1626209/100‑1 (MQ=255)

                                                                     atatTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGc                         >  2:457266/1‑100 (MQ=255)

                                                                     atatTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGc                         <  1:405942/100‑1 (MQ=255)

                                                                      tatTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCa                        >  1:965621/1‑100 (MQ=255)

                                                                       atTGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCag                       <  1:1376376/100‑1 (MQ=255)

                                                                        ttGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCTGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCgttgt                      <  2:843683/100‑5 (MQ=255)

                                                                        ttGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCagt                      <  1:651474/100‑1 (MQ=255)

                                                                        ttGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCagt                      <  1:3821770/100‑1 (MQ=255)

                                                                         tGAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGCTTATTGTCGCagta                     >  2:1045126/1‑100 (MQ=255)

                                                                          gAAGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCagtag                    >  1:2707270/1‑100 (MQ=255)

                                                                           aaGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCagtagt                   <  1:4301553/100‑1 (MQ=255)

                                                                            ngctgctAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGtt                  >  1:4320138/2‑100 (MQ=255)

                                                                            aGCTGCTAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGtt                  >  1:162693/1‑100 (MQ=255)

                                                                             gctgctAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTg                 <  1:2217978/100‑1 (MQ=255)

                                                                              ctgctAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGc                <  1:4386144/100‑1 (MQ=255)

                                                                              ctgctAAAGAAGAAGGGGGCACCATGTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAGTTTCCGGGATTATTGTGGCAGTAGTTGc                <  2:3733748/100‑1 (MQ=255)

                                                                               tgctAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCg               <  1:3876461/100‑1 (MQ=255)

                                                                               tgctAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCg               <  2:1618736/100‑1 (MQ=255)

                                                                                gctAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGa              <  2:3361379/100‑1 (MQ=255)

                                                                                 ctAAAGCAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCACCAGCAAGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGAt             >  2:820607/1‑100 (MQ=255)

                                                                                  tAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATg            <  1:357181/100‑1 (MQ=255)

                                                                                  tAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATg            >  2:2979726/1‑100 (MQ=255)

                                                                                  tAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATg            >  1:3217613/1‑100 (MQ=255)

                                                                                  tAAAGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATg            >  1:3991037/1‑100 (MQ=255)

                                                                                   aaaGAAGAAGGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGt           >  2:3991022/1‑100 (MQ=255)

                                                                                     aggagaagGGGGCACCATTTTATCGGCGCTTTCGATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCg         <  1:3830168/97‑1 (MQ=255)

                                                                                        agaagGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGaaa      >  1:2242803/1‑100 (MQ=255)

                                                                                        agaagGGGGCACCATTTTATCGGCGCTTCCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGaaa      >  1:4020216/1‑100 (MQ=255)

                                                                                         gaagGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAAt     <  1:3904664/100‑1 (MQ=255)

                                                                                         gaagGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAAt     <  2:3055225/100‑1 (MQ=255)

                                                                                         gaagGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAAt     <  2:3616827/100‑1 (MQ=255)

                                                                                          aagGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATc    <  1:3724673/100‑1 (MQ=255)

                                                                                          aagGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATc    <  1:2340021/100‑1 (MQ=255)

                                                                                           agGGGGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTATCGCAGTAGTTGCGATGTCGAAATCa   >  1:1121775/1‑100 (MQ=255)

                                                                                            ggggGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGCAATCAt  >  1:3672066/1‑100 (MQ=255)

                                                                                            ggggGCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCAt  >  1:3266286/1‑100 (MQ=255)

CTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCGGGATTATTGTCGCAGTAGTTGCGATGTCGAAATCAT  >  NC_000913/2986556‑2986747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`