BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,224,239	0	A	T	21.9%	270.5 / 72.8	129	intergenic (+332/+40)	ycgI/minE	pseudogene/cell division topological specificity factor
Reads supporting (aligned to +/- strand): ref base A (51/49); new base T (14/14); total (66/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 138 total aligned reads displayed.

GTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGC  >  NC_000913/1224141‑1224337

gtAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAgaga                                                                                                    <  2:2026152/99‑1 (MQ=255)

gtAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAgaga                                                                                                    <  2:2028008/99‑1 (MQ=255)

 tAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGAt                                                                                                   >  2:1586397/1‑99 (MQ=255)

  aaaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATa                                                                                                  >  1:640633/1‑99 (MQ=255)

   aaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTat                                                                                                 <  2:1790080/99‑2 (MQ=255)

   aaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATaa                                                                                                 >  1:1200051/1‑99 (MQ=255)

     gggggAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAAct                                                                                               >  1:1568520/1‑99 (MQ=255)

       gggAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAActct                                                                                             <  2:1015953/99‑1 (MQ=255)

        ggAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTg                                                                                            <  2:805435/99‑1 (MQ=255)

         gAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGc                                                                                           <  1:1696947/99‑1 (MQ=255)

         gAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGc                                                                                           <  1:1898970/99‑1 (MQ=255)

          aGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGcc                                                                                          <  1:942006/99‑1 (MQ=255)

          aGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGcc                                                                                          <  1:264550/99‑1 (MQ=255)

          aGGTGGAAATAGCAAAGAGGAGCATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGcc                                                                                          <  2:2198658/99‑1 (MQ=255)

           ggTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCt                                                                                         <  1:2693910/99‑1 (MQ=255)

            gTGGTAATAGCCATGAGGAGTATCAGCAAGAATACTCGCAGCTTTACCACAACGTGGATGTGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCtt                                                                                        >  2:3296636/1‑99 (MQ=255)

            gTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGTGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCtt                                                                                        >  2:3296795/1‑99 (MQ=255)

            gTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCtt                                                                                        <  1:1467676/99‑1 (MQ=255)

            gTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCtt                                                                                        <  1:2212010/99‑1 (MQ=255)

            gTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCtt                                                                                        <  1:1306162/99‑1 (MQ=255)

              ggAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGa                                                                                      <  1:328936/99‑1 (MQ=255)

               gAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGag                                                                                     <  2:989688/99‑2 (MQ=255)

               gAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGaa                                                                                     <  2:1374018/99‑1 (MQ=255)

               gAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGaa                                                                                     <  1:864597/99‑1 (MQ=255)

               gAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGaa                                                                                     >  2:270713/1‑99 (MQ=255)

               gAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGaa                                                                                     <  1:516292/99‑1 (MQ=255)

                aaaTAGCAATGAGGAGTATCAGCAAGAAGCCTCGCCGCTTTACCACAACGTCGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagt                                                                                    <  2:2165668/99‑3 (MQ=38)

                 aaTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagtt                                                                                   <  2:589348/99‑4 (MQ=38)

                  aTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGAt                                                                                  >  1:2264752/1‑99 (MQ=255)

                  aTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGAt                                                                                  <  1:2867662/99‑1 (MQ=255)

                    aGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATaa                                                                                >  1:122817/1‑99 (MQ=255)

                     gCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATaaa                                                                               <  1:1107788/99‑1 (MQ=255)

                     gCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATaaa                                                                               <  1:1107011/99‑1 (MQ=255)

                      cAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAAt                                                                              >  2:1493969/1‑99 (MQ=255)

                         tGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATgcg                                                                           >  1:179879/1‑99 (MQ=255)

                          gaggagTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATgcgc                                                                          >  1:3398339/1‑99 (MQ=255)

                             gagTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACGCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCttt                                                                       >  1:160496/1‑99 (MQ=255)

                                    aGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCg                                                                >  1:3466432/1‑99 (MQ=255)

                                     gCAAGAATACTCGCCGCTTTACCACAACGTGGCTGAGAGGGATGAAAACCTCAAGGCAGAGCTACCTCTGCCTTGAAGATAAATGCGCCTTTACAGCgg                                                               >  2:2058470/1‑99 (MQ=255)

                                      cAAGATTACTCGCAGCTTTACCACAACGTGGATGAGCTGGATGTAAAACTCATGGCAGAGTTAACTATGCCTTGAAGATAAATGCGCTTTTACAGCggg                                                              <  2:123170/99‑1 (MQ=255)

                                      cAAGAATACTCGCCACTTTACCACAACGTAGATGAGAGGGATGATAAACTCAAGGCTGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCggg                                                              >  1:3026012/1‑99 (MQ=255)

                                        aGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCt                                                            >  1:1608727/1‑99 (MQ=255)

                                          aaTACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTa                                                          <  2:1628945/99‑1 (MQ=255)

                                            tACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAtt                                                        <  1:2883125/99‑1 (MQ=255)

                                             aCTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAtat                                                       <  2:1886052/99‑3 (MQ=255)

                                              cTCGCCGCTTTACCACAACGTGGATGAGATGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTc                                                      <  1:1620333/99‑1 (MQ=255)

                                               tCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCa                                                     >  1:2038959/1‑99 (MQ=255)

                                                cgccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAg                                                    <  2:2450627/99‑1 (MQ=255)

                                                 gccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGc                                                   >  2:284202/1‑99 (MQ=255)

                                                     cTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtctt                                               >  1:619332/1‑99 (MQ=255)

                                                      tttACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtcttc                                              <  1:1515554/99‑1 (MQ=255)

                                                         aCCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGc                                           >  1:2128852/1‑99 (MQ=255)

                                                          ccACAACGTTGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTTAAGAGCAATGCGCTTTTAAAGCGGGCTTCTTTCTGCTCTTCTGCt                                          >  2:222610/1‑99 (MQ=255)

                                                          ccACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCt                                          >  2:2301387/1‑99 (MQ=255)

                                                           cacaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGGGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCtt                                         >  2:3380707/1‑99 (MQ=255)

                                                           cacaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCtt                                         >  1:871411/1‑99 (MQ=255)

                                                           cacaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCtt                                         >  1:623895/1‑99 (MQ=255)

                                                             caACGTGGATGAGAGGGATGAAATACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTcc                                       <  1:2170893/99‑1 (MQ=255)

                                                              aaCGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCg                                      <  1:2922234/99‑1 (MQ=255)

                                                              aaCGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCg                                      <  1:3126747/99‑1 (MQ=255)

                                                                cGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATCACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGt                                    <  1:3217316/99‑1 (MQ=255)

                                                                 gTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTa                                   >  1:1518580/1‑99 (MQ=255)

                                                                  tGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTaa                                  <  1:1367682/99‑1 (MQ=255)

                                                                  tGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTaa                                  >  1:1212959/1‑99 (MQ=255)

                                                                  tGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTaa                                  <  1:2471974/99‑1 (MQ=255)

                                                                   ggATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAg                                 <  1:3247828/99‑1 (MQ=255)

                                                                   ggATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAg                                 >  2:2189564/1‑99 (MQ=255)

                                                                      tGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTc                              >  1:158609/1‑99 (MQ=255)

                                                                      tGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTc                              >  2:3250683/1‑99 (MQ=255)

                                                                          agGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGt                          >  1:1139511/1‑99 (MQ=255)

                                                                           gggATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGtt                         <  2:523689/99‑1 (MQ=255)

                                                                             gATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTGTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCa                       <  1:1381590/99‑1 (MQ=255)

                                                                              aTGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAATATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAg                      >  2:2296385/1‑99 (MQ=255)

                                                                              aTGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAg                      >  2:683957/1‑99 (MQ=255)

                                                                              aTGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAg                      <  2:2713046/99‑1 (MQ=255)

                                                                              aTGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAg                      >  2:2378609/1‑99 (MQ=255)

                                                                                    aaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAg                >  2:1874958/1‑99 (MQ=255)

                                                                                     cttCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGa               >  1:538858/3‑99 (MQ=39)

                                                                                     cttCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGa               >  2:2541525/3‑99 (MQ=255)

                                                                                      ttCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGaa              >  1:531880/2‑99 (MQ=255)

                                                                                       gcAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAAt             <  2:268959/98‑1 (MQ=255)

                                                                                        cAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATa            >  1:414562/1‑99 (MQ=255)

                                                                                        cAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATa            <  1:1759844/99‑1 (MQ=255)

                                                                                        cAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATa            <  1:1759689/99‑1 (MQ=255)

                                                                                          aGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGa          >  1:3328120/1‑99 (MQ=255)

                                                                                          aGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGa          >  2:3134651/1‑99 (MQ=255)

                                                                                          aGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGa          >  2:1774633/1‑99 (MQ=255)

                                                                                          aGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGa          >  1:426705/1‑99 (MQ=255)

                                                                                          aGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGa          >  2:423695/1‑99 (MQ=255)

                                                                                          aGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGa          >  2:2213602/1‑99 (MQ=255)

                                                                                           ggCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGaa         >  2:1927446/1‑99 (MQ=255)

                                                                                           ggCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGAAAGGTCACGTTCAGCTCAAGAATAGaa         >  2:1562840/1‑99 (MQ=255)

                                                                                            gCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGaaa        <  1:3337198/99‑1 (MQ=255)

                                                                                            gCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGaaa        >  1:1163125/1‑99 (MQ=255)

                                                                                                agaTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATc    >  2:569680/1‑99 (MQ=255)

                                                                                                aaaTAACTCTGCATTGAATATAAATGCTCTTTTACAGCGCGCTTATTTCATCTCTTATCCTTCCGGTTAGGTCAACTTCAGCTCCAGAATAGAAATATc    <  2:858291/97‑1 (MQ=255)

                                                                                                  aTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGc  >  2:1658201/1‑99 (MQ=255)

                                                                                                  aTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGc  <  2:748767/99‑1 (MQ=255)

                                                                                                  aTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGc  <  1:1234333/99‑1 (MQ=255)

                                                                                                  aTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGc  <  1:3042027/99‑1 (MQ=255)

GTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGC  >  NC_000913/1224141‑1224337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`