BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,674,955	0	A	T	23.3%	258.6 / 82.8	132	intergenic (+8/+17)	tqsA/pntB	pheromone AI‑2 transporter/pyridine nucleotide transhydrogenase, beta subunit
Reads supporting (aligned to +/- strand): ref base A (54/45); new base T (15/15); total (71/61)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 140 total aligned reads displayed.

GTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGG  >  NC_000913/1674860‑1675053

gTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGCTAAGCGATCTCAATAAAGAGTGACGGCCTcagca                                                                                                 <  2:3336926/99‑1 (MQ=255)

 ttGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagcag                                                                                                >  1:1575833/1‑99 (MQ=255)

 ttGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagcag                                                                                                >  2:2528113/1‑99 (MQ=255)

  tGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCaga                                                                                               <  1:1904274/99‑1 (MQ=255)

  tGACAATTATAGTCAAAATTGCGCTTGAACAAACAGCGGGAGAACAAAGCATCGCCGTTCTGTTAAGCGGTCTCAATAAAGAGTGACGGCCTCAACaga                                                                                               <  2:164964/99‑1 (MQ=255)

     cAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGc                                                                                            >  1:734271/1‑99 (MQ=255)

      aaTTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCCGCAGAGGcc                                                                                           >  2:2969593/1‑99 (MQ=255)

      aaTTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGcc                                                                                           >  2:2789550/1‑99 (MQ=255)

      aaTTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGACAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAAAGGcc                                                                                           >  1:1185299/1‑99 (MQ=255)

       attattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCg                                                                                          <  1:2265249/99‑1 (MQ=255)

       attattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCg                                                                                          >  1:27051/1‑99 (MQ=255)

        ttattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGt                                                                                         <  2:3000691/99‑1 (MQ=255)

        ttattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGt                                                                                         <  1:98755/99‑1 (MQ=255)

        ttattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGt                                                                                         <  1:1816525/99‑1 (MQ=255)

        ttattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGt                                                                                         <  1:1831566/99‑1 (MQ=255)

        ttattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGt                                                                                         >  2:2718713/1‑99 (MQ=255)

          attGTCAAAATTGCGCTTGGACAAACCGCGGGATGTCGAAGGAGCGCCGTTCTGTTATGCGATCTGAATAAAGAGTGACGGCCTCGGCTGAGGCCGTCa                                                                                       <  2:2172656/99‑1 (MQ=255)

          attGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCa                                                                                       <  2:1726354/99‑1 (MQ=255)

          attGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCa                                                                                       <  1:1078323/99‑1 (MQ=255)

          atggTCAAAATTGCGCTTGAACAAACAGCGGGAGGTGAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGACTCTGCTGAGGCCGTCa                                                                                       <  2:2173195/96‑1 (MQ=255)

           ttGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                      <  1:1425356/99‑2 (MQ=255)

           ttGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                      <  2:2364137/99‑2 (MQ=255)

           ttGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAg                                                                                      <  2:1196572/99‑1 (MQ=255)

           ttGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAg                                                                                      >  1:3270473/1‑99 (MQ=255)

           ttGTCAAAATTGCCCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                      <  2:1628077/99‑2 (MQ=39)

             gTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCactc                                                                                    <  1:2429478/99‑4 (MQ=39)

              tCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGt                                                                                   >  2:1703593/1‑99 (MQ=255)

              tCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGt                                                                                   >  1:846183/1‑99 (MQ=255)

              tCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGt                                                                                   >  1:1306735/1‑99 (MQ=255)

                aaaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTa                                                                                 >  1:606452/1‑99 (MQ=255)

                aaaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTa                                                                                 >  2:3154209/1‑99 (MQ=255)

                aaaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTa                                                                                 >  1:216780/1‑99 (MQ=255)

                aaaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTa                                                                                 >  1:997086/1‑99 (MQ=255)

                     tGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACagag                                                                            <  1:2433057/99‑1 (MQ=255)

                        gcTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCtt                                                                         >  2:2447961/1‑99 (MQ=255)

                         cTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCttt                                                                        >  2:324065/1‑99 (MQ=255)

                         cTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCttt                                                                        >  2:2226181/1‑99 (MQ=255)

                         cTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCATCAGCAGAGGCCGTCAGGGTTACAGAGCttt                                                                        >  1:1237709/1‑99 (MQ=255)

                          ttGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTAAGGGTTACAGAGCTTTc                                                                       >  1:787540/1‑99 (MQ=255)

                            gAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAg                                                                     >  2:2720821/1‑99 (MQ=255)

                             aacaaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAgg                                                                    >  1:1731577/1‑99 (MQ=255)

                              acaaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGa                                                                   >  2:1538541/1‑99 (MQ=255)

                                   caGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCa                                                              <  1:2096661/99‑1 (MQ=255)

                                    aGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCAt                                                             <  1:680594/99‑1 (MQ=255)

                                           ggTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCt                                                      <  2:3216671/99‑1 (MQ=255)

                                           ggTCAAAGCATCGCCGTTCTGTTAAGCGATCGCAATAAAGAGTGACCGCCTCAGCAGAGGTCGGCAGGGTTACAGAGCTTTCCGGACTGCATCCACGCt                                                      >  2:2228502/1‑99 (MQ=255)

                                             tCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTgg                                                    <  1:2043536/99‑1 (MQ=255)

                                              cAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAACGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGc                                                   >  2:3209992/1‑99 (MQ=255)

                                              cAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGc                                                   <  2:1945638/99‑1 (MQ=255)

                                              cAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGc                                                   <  1:1082487/99‑1 (MQ=255)

                                               aaaGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTAAAGAGCTTTCAGGATTGCATCCACGCTGGCt                                                  >  1:3313251/1‑99 (MQ=255)

                                                   cATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTgg                                              <  2:1221123/99‑1 (MQ=255)

                                                     tCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCCCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCTGGATTGCATCCACCCTGGCTTTGGCg                                            <  1:3067925/99‑1 (MQ=255)

                                                       gCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTc                                          >  2:466911/1‑99 (MQ=255)

                                                         cGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCAc                                        <  1:2999194/99‑1 (MQ=255)

                                                           ttCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCa                                      >  2:714404/1‑99 (MQ=255)

                                                                ttAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAg                                 >  1:767745/1‑99 (MQ=255)

                                                                 tAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGc                                >  2:2166582/1‑99 (MQ=255)

                                                                 tAAGCGATCTCAATAAAGAGGGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCAACCACGCTGGCTTTAGCGTCACCAAACAGc                                <  2:2522536/99‑1 (MQ=255)

                                                                  aaGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCa                               >  2:467709/1‑99 (MQ=255)

                                                                     cGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAtgt                            <  2:1734980/99‑1 (MQ=255)

                                                                     cGATCCCAATAAACAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTGCAGAGCTTTCAGGATTGCATCCACGCTCGCTTTGGCGTCGCCAAACAGCAtgt                            <  2:3195865/99‑1 (MQ=255)

                                                                       aTCTCAATAAAGATTGACGGCCTCAGCAGAGGCCGTCAGGGTTACCGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTgg                          <  1:2813574/99‑1 (MQ=255)

                                                                       aTCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTgg                          <  1:2560488/99‑1 (MQ=255)

                                                                       aTCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTgg                          <  1:2022488/99‑1 (MQ=255)

                                                                       aTCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTgg                          <  2:3411743/99‑1 (MQ=255)

                                                                       aTCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTgg                          <  1:832162/99‑1 (MQ=255)

                                                                        tctcAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTggg                         >  2:134905/1‑99 (MQ=255)

                                                                          tcAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCGACGCTGGCTTTGGCGTCACCAAACAGCATGTGGgtg                       >  1:3272129/1‑99 (MQ=255)

                                                                           cAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGgtgt                      <  1:2268189/99‑1 (MQ=255)

                                                                              tAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGtttt                   <  2:2263744/99‑1 (MQ=255)

                                                                               aaaGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTc                  <  1:3356406/99‑1 (MQ=255)

                                                                                 atagTGACGGCCTCAGCAGAGGCCGTCATGGTTACAGAGCTTTCAGTATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCt                <  2:2470358/97‑1 (MQ=255)

                                                                                 agagTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCt                <  2:940702/99‑1 (MQ=255)

                                                                                  gagTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCtt               >  2:2776759/1‑99 (MQ=255)

                                                                                  gagTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCtt               <  1:2977418/99‑1 (MQ=255)

                                                                                  ccctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCtt               >  2:1192549/4‑99 (MQ=39)

                                                                                  ccctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCtt               >  2:506854/4‑99 (MQ=39)

                                                                                   cctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTg              >  1:330953/3‑99 (MQ=39)

                                                                                    gTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTga             >  2:1965958/1‑99 (MQ=255)

                                                                                    gTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTga             <  2:1942623/99‑1 (MQ=255)

                                                                                    ctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTga             >  1:2851458/2‑99 (MQ=255)

                                                                                    ctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTACTTga             >  1:1302691/2‑99 (MQ=39)

                                                                                    ctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGAATCCGCGCTGGCTTTGGCGCCACCAAACAGCATGTGGGTGTTTACCTTga             >  2:3223847/2‑99 (MQ=38)

                                                                                     tGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa            >  1:109872/1‑99 (MQ=255)

                                                                                     tGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa            >  1:1980450/1‑99 (MQ=255)

                                                                                     tGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa            >  1:2357934/1‑99 (MQ=255)

                                                                                     tGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa            >  2:2684076/1‑99 (MQ=255)

                                                                                     tGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCGGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa            >  2:3309991/1‑99 (MQ=255)

                                                                                       aCGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaaga          >  1:1604784/1‑99 (MQ=255)

                                                                                       aCGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaaga          >  1:685647/1‑99 (MQ=255)

                                                                                       aCGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaaga          >  2:62261/1‑99 (MQ=255)

                                                                                         ggCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAAc        >  2:3151736/1‑99 (MQ=255)

                                                                                           ccTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAg      <  1:3335157/99‑1 (MQ=255)

                                                                                            cTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGc     <  1:1743560/99‑1 (MQ=255)

                                                                                            cTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGc     <  2:607735/99‑1 (MQ=255)

                                                                                            cTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGACACCAAACAGCATGTGGGTGTTTTCCTTGTAGAACAGc     >  1:3321911/1‑99 (MQ=255)

                                                                                             tCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCg    >  2:230625/1‑99 (MQ=255)

                                                                                              cagcagAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCgg   <  1:2523112/99‑1 (MQ=255)

                                                                                               agcagAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAACCAGCATGTGGGTGTTTTCCTTGAAGAACAGCggg  >  2:3026006/1‑99 (MQ=255)

GTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGG  >  NC_000913/1674860‑1675053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`