BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,674,958	0	A	T	24.0%	249.2 / 82.8	129	intergenic (+11/+14)	tqsA/pntB	pheromone AI‑2 transporter/pyridine nucleotide transhydrogenase, beta subunit
Reads supporting (aligned to +/- strand): ref base A (56/42); new base T (15/16); total (71/58)
Fisher's exact test for biased strand distribution p-value = 4.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 143 total aligned reads displayed.

GTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTT  >  NC_000913/1674860‑1675056

gTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGCTAAGCGATCTCAATAAAGAGTGACGGCCTcagca                                                                                                    <  2:3336926/99‑1 (MQ=255)

 ttGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagcag                                                                                                   >  2:2528113/1‑99 (MQ=255)

 ttGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagcag                                                                                                   >  1:716550/1‑99 (MQ=255)

 ttGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagcag                                                                                                   >  1:1575833/1‑99 (MQ=255)

  tGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCaga                                                                                                  <  1:1904274/99‑1 (MQ=255)

  tGACAATTATAGTCAAAATTGCGCTTGAACAAACAGCGGGAGAACAAAGCATCGCCGTTCTGTTAAGCGGTCTCAATAAAGAGTGACGGCCTCAACaga                                                                                                  <  2:164964/99‑1 (MQ=255)

     cAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGc                                                                                               >  1:734271/1‑99 (MQ=255)

      aaTTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCCGCAGAGGcc                                                                                              >  2:2969593/1‑99 (MQ=255)

      aaTTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGcc                                                                                              >  2:2789550/1‑99 (MQ=255)

      aaTTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGACAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAAAGGcc                                                                                              >  1:1185299/1‑99 (MQ=255)

       attattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCg                                                                                             >  1:27051/1‑99 (MQ=255)

       attagtGTCAAAAGTGCGCTTGAACCAACAGCGGGATGTCAAAGCAGCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCg                                                                                             <  1:2933423/99‑1 (MQ=255)

        ttattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGt                                                                                            <  2:1259045/99‑1 (MQ=255)

        ttattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGt                                                                                            <  1:1816525/99‑1 (MQ=255)

        ttattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGt                                                                                            >  2:2718713/1‑99 (MQ=255)

          attGTCAAAATTGCGCTTGGACAAACCGCGGGATGTCGAAGGAGCGCCGTTCTGTTATGCGATCTGAATAAAGAGTGACGGCCTCGGCTGAGGCCGTCa                                                                                          <  2:2172656/99‑1 (MQ=255)

          attGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCa                                                                                          <  1:1078323/99‑1 (MQ=255)

           ttGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                         <  2:2364137/99‑2 (MQ=255)

           ttGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAg                                                                                         >  1:3270473/1‑99 (MQ=255)

            tGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAgg                                                                                        <  1:2197541/99‑1 (MQ=255)

              tCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCATAGGCCGTGAggga                                                                                      >  2:3191367/1‑98 (MQ=255)

              tCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGt                                                                                      >  1:846183/1‑99 (MQ=255)

              tCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGt                                                                                      >  2:1703593/1‑99 (MQ=255)

              tCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGt                                                                                      >  1:1306735/1‑99 (MQ=255)

                aaaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTa                                                                                    >  1:216780/1‑99 (MQ=255)

                aaaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTa                                                                                    >  1:606452/1‑99 (MQ=255)

                aaaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTa                                                                                    >  2:3154209/1‑99 (MQ=255)

                aaaaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTa                                                                                    >  1:997086/1‑99 (MQ=255)

                  aagtGCGCTTGAACAAACAGCGGGAGGTCAAAGCCTCGCCGTTCTGTTAAGCGATCTCAATCAAGAGTGACGGCCTCAGCAGAGCCCGTCAGGGTTACa                                                                                  <  2:1721315/96‑1 (MQ=255)

                     tGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACagag                                                                               <  1:2433057/99‑1 (MQ=255)

                        gcTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTGAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCtt                                                                            >  1:182872/1‑99 (MQ=255)

                        gcTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCtt                                                                            >  2:2447961/1‑99 (MQ=255)

                         cTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCttt                                                                           >  2:2226181/1‑99 (MQ=255)

                         cTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCttt                                                                           >  2:324065/1‑99 (MQ=255)

                         cTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCATCAGCAGAGGCCGTCAGGGTTACAGAGCttt                                                                           >  1:1237709/1‑99 (MQ=255)

                          ttGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTc                                                                          >  2:2755843/1‑99 (MQ=255)

                          ttGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTAAGGGTTACAGAGCTTTc                                                                          >  1:787540/1‑99 (MQ=255)

                             aacaaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAgg                                                                       >  1:1731577/1‑99 (MQ=255)

                              acaaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGa                                                                      >  2:1538541/1‑99 (MQ=255)

                               caaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGGGGCCGTCAGGGTTACAGAGCTTTCAGGAt                                                                     >  1:1467994/1‑99 (MQ=255)

                                aaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGAtt                                                                    >  1:2071758/1‑99 (MQ=255)

                                  acaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGc                                                                  >  1:2462044/1‑99 (MQ=255)

                                  acaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCACAATAAAGAGTGACGTCCTCCGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGc                                                                  >  2:1569601/1‑99 (MQ=255)

                                           ggTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCt                                                         <  2:3216671/99‑1 (MQ=255)

                                           ggTCAAAGCATCGCCGTTCTGTTAAGCGATCGCAATAAAGAGTGACCGCCTCAGCAGAGGTCGGCAGGGTTACAGAGCTTTCCGGACTGCATCCACGCt                                                         >  2:2228502/1‑99 (MQ=255)

                                             tCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTgg                                                       <  1:2043536/99‑1 (MQ=255)

                                             tCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTgg                                                       >  1:901869/1‑99 (MQ=255)

                                              cAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGc                                                      <  1:1082487/99‑1 (MQ=255)

                                              cAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGc                                                      <  2:1945638/99‑1 (MQ=255)

                                              cAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGc                                                      >  1:400478/1‑99 (MQ=255)

                                               aaaGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCt                                                     >  2:3100125/1‑99 (MQ=255)

                                               aaaGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCt                                                     >  1:2408271/1‑99 (MQ=255)

                                               aaaGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCt                                                     >  2:1916474/1‑99 (MQ=255)

                                               aaaGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTAAAGAGCTTTCAGGATTGCATCCACGCTGGCt                                                     >  1:3313251/1‑99 (MQ=255)

                                                   cATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTgg                                                 <  2:1221123/99‑1 (MQ=255)

                                                    aTCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGc                                                <  2:491652/99‑1 (MQ=255)

                                                     tCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCCCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCTGGATTGCATCCACCCTGGCTTTGGCg                                               <  1:3067925/99‑1 (MQ=255)

                                                         cGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCAc                                           <  1:2999194/99‑1 (MQ=255)

                                                         cGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCAc                                           <  1:196909/99‑1 (MQ=255)

                                                           ttCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCa                                         >  2:714404/1‑99 (MQ=255)

                                                                ttAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAg                                    >  2:3278021/1‑99 (MQ=255)

                                                                 tAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGc                                   >  2:2166582/1‑99 (MQ=255)

                                                                 tAAGCGATCTCAATAAAGAGGGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCAACCACGCTGGCTTTAGCGTCACCAAACAGc                                   <  2:2522536/99‑1 (MQ=255)

                                                                  aaGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCa                                  >  2:467709/1‑99 (MQ=255)

                                                                  aaGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCa                                  <  1:776251/99‑1 (MQ=255)

                                                                     cGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAtgt                               <  2:1734980/99‑1 (MQ=255)

                                                                     cGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAtgt                               <  2:2172937/99‑1 (MQ=255)

                                                                     cGATCCCAATAAACAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTGCAGAGCTTTCAGGATTGCATCCACGCTCGCTTTGGCGTCGCCAAACAGCAtgt                               <  2:3195865/99‑1 (MQ=255)

                                                                       aTCTCAATAAAGATTGACGGCCTCAGCAGAGGCCGTCAGGGTTACCGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTgg                             <  1:2813574/99‑1 (MQ=255)

                                                                       aTCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTgg                             <  1:2560488/99‑1 (MQ=255)

                                                                       aTCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTgg                             <  2:3411743/99‑1 (MQ=255)

                                                                          tcAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCGACGCTGGCTTTGGCGTCACCAAACAGCATGTGGgtg                          >  1:3272129/1‑99 (MQ=255)

                                                                           cAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGgtgt                         <  1:2268189/99‑1 (MQ=255)

                                                                             aTAAAGTGTGACGGCCTCAGCAGAGGCCGTTAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGCTGttt                       <  2:3218321/99‑1 (MQ=255)

                                                                              tAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGtttt                      <  2:424757/99‑1 (MQ=255)

                                                                              tAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGtttt                      <  2:2263744/99‑1 (MQ=255)

                                                                               aaaGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTc                     <  1:3356406/99‑1 (MQ=255)

                                                                                 atagTGACGGCCTCAGCAGAGGCCGTCATGGTTACAGAGCTTTCAGTATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCt                   <  2:2470358/97‑1 (MQ=255)

                                                                                 agagTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCt                   <  2:940702/99‑1 (MQ=255)

                                                                                  gagTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCtt                  >  2:2776759/1‑99 (MQ=255)

                                                                                  ccctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCtt                  >  2:1192549/4‑99 (MQ=39)

                                                                                   cctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTg                 >  1:330953/3‑99 (MQ=39)

                                                                                    gTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTga                >  2:1965958/1‑99 (MQ=255)

                                                                                    ctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTga                >  1:2851458/2‑99 (MQ=255)

                                                                                    ctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTACTTga                >  1:1302691/2‑99 (MQ=39)

                                                                                     tGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa               >  2:2684076/1‑99 (MQ=255)

                                                                                     tGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa               >  1:109872/1‑99 (MQ=255)

                                                                                     tGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa               >  1:1980450/1‑99 (MQ=255)

                                                                                     tGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa               >  1:2357934/1‑99 (MQ=255)

                                                                                     tGACGGCCTCTGCTGAGCCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGGGGGTGTTTTCCTTgaa               >  2:1102861/1‑99 (MQ=255)

                                                                                       aCGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaaga             >  1:1604784/1‑99 (MQ=255)

                                                                                       aCGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaaga             >  1:685647/1‑99 (MQ=255)

                                                                                       aCGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaaga             >  2:62261/1‑99 (MQ=255)

                                                                                           ccTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAg         <  1:3335157/99‑1 (MQ=255)

                                                                                            cTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGc        <  1:1743560/99‑1 (MQ=255)

                                                                                            cTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGc        <  2:607735/99‑1 (MQ=255)

                                                                                            cTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGACACCAAACAGCATGTGGGTGTTTTCCTTGTAGAACAGc        >  1:3321911/1‑99 (MQ=255)

                                                                                             tCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCg       >  2:230625/1‑99 (MQ=255)

                                                                                               agcagAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAACCAGCATGTGGGTGTTTTCCTTGAAGAACAGCggg     >  2:3026006/1‑99 (MQ=255)

                                                                                                 cagAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGtt   <  2:2999281/99‑1 (MQ=255)

GTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTT  >  NC_000913/1674860‑1675056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`