BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,439,588	0	G	C	22.9%	369.6 / 115.6	176	intergenic (‑79/+28)	yhdN/rplQ	DUF1992 family protein/50S ribosomal subunit protein L17
Reads supporting (aligned to +/- strand): ref base G (70/65); new base C (23/17); total (94/82)
Fisher's exact test for biased strand distribution p-value = 5.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.20e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 189 total aligned reads displayed.

CACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTC  >  NC_000913/3439490‑3439684

cACTGGTCAAGTAACCACATAAATACTCAAGGGGGTGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACAACGGGTATAAAAAAACacgccg                                                                                                  >  1:3165765/1‑99 (MQ=255)

  cTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCggg                                                                                                >  1:1796453/1‑99 (MQ=255)

      tCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCgg                                                                                            <  1:1621020/99‑1 (MQ=255)

       cAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCggg                                                                                           >  2:1297731/1‑99 (MQ=255)

           tAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCCCGGCGGGtttt                                                                                       <  1:1411348/99‑1 (MQ=255)

            aaCCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGttttt                                                                                      <  2:2659489/99‑1 (MQ=255)

            aaCCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCCCGGCGGGttttt                                                                                      <  1:290002/99‑1 (MQ=255)

             aCCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGtttttt                                                                                     >  1:2382384/1‑99 (MQ=255)

             aCCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGtttttt                                                                                     <  1:3272050/99‑1 (MQ=255)

             aCCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGtttttt                                                                                     >  1:2607292/1‑99 (MQ=255)

             aCCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGtttttt                                                                                     <  2:2762532/99‑1 (MQ=255)

             aCCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCCCGGCGGGtttttt                                                                                     <  2:1059558/99‑1 (MQ=255)

             aCCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCCCGGCGGGtttttt                                                                                     <  2:985988/99‑1 (MQ=255)

             aCCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCCCGGCGGGtttttt                                                                                     <  1:2802032/99‑1 (MQ=255)

              ccACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCCCGGCGGGttttttt                                                                                    <  1:2467698/99‑1 (MQ=255)

              ccACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCCCGGCGGGttttttt                                                                                    <  1:1521228/99‑1 (MQ=255)

               cacaTATATACTCCTGTTGATGAACAAAAAGATTTCATCTATTGTAGATAAGTGGGTATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTa                                                                                   <  1:861447/99‑1 (MQ=255)

               cacaTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTa                                                                                   <  1:734085/99‑1 (MQ=255)

               cacaTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTa                                                                                   >  1:2989156/1‑99 (MQ=255)

               cacaTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTa                                                                                   >  1:396964/1‑99 (MQ=255)

               cacaTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTa                                                                                   <  1:1118857/99‑1 (MQ=255)

               cacaTAAATACGCCAGGGGATGAAAAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTa                                                                                   <  1:1147289/99‑1 (MQ=255)

                acaTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTAc                                                                                  >  1:528457/1‑99 (MQ=255)

                acaTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTAc                                                                                  <  1:3435329/99‑1 (MQ=255)

                 caTAAATACTCCAGGGGATTAACAAAAAGAGTACAGCTATTGTAGATAAGTTGTGATACTACGGGTATAAAAAATCCCGCCCCGGCGGGTTTTTTTata                                                                                 <  1:2948744/99‑3 (MQ=37)

                  aTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGt                                                                                >  1:514347/1‑99 (MQ=255)

                  aTAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGt                                                                                <  1:2073852/99‑1 (MQ=255)

                     aaTACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGc                                                                             <  1:2216922/99‑1 (MQ=255)

                        aCTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTc                                                                          >  1:226972/1‑99 (MQ=255)

                          tCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAg                                                                        <  2:2038426/99‑1 (MQ=255)

                          tCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAg                                                                        >  2:2154423/1‑99 (MQ=255)

                            cAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGAt                                                                      >  1:1702613/1‑99 (MQ=255)

                              ggggATGAACAAAAAGAGTACAGCTATTGTAGATAAGGGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTa                                                                    <  1:318399/99‑1 (MQ=255)

                                ggATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTAct                                                                  >  1:2411763/1‑99 (MQ=255)

                                ggATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTAct                                                                  <  2:473771/99‑1 (MQ=255)

                                 gATGAACAAAAAGACTACCGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTAcac                                                                 <  2:202089/99‑3 (MQ=255)

                                    gAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTgc                                                              <  2:2883800/99‑1 (MQ=255)

                                     aaCAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTgca                                                             <  1:264610/99‑1 (MQ=255)

                                      aCAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTgcag                                                            >  2:770645/1‑99 (MQ=255)

                                      aCAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTgcag                                                            >  1:151283/1‑99 (MQ=255)

                                       cAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTgcagc                                                           >  2:1600632/1‑99 (MQ=255)

                                        aaaaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTgcagca                                                          <  1:2139176/99‑1 (MQ=255)

                                          aaaGAGTACAGGTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTGCAGAGTAATCTgaagcaac                                                        <  2:2693792/99‑3 (MQ=255)

                                          aaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCagcagc                                                        >  2:1727001/1‑99 (MQ=255)

                                          aaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCagcagc                                                        <  1:1502957/99‑1 (MQ=255)

                                               gTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTg                                                   >  1:124200/1‑99 (MQ=255)

                                                tACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGc                                                  <  1:2020180/99‑1 (MQ=255)

                                                tACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGc                                                  >  2:1471008/1‑99 (MQ=255)

                                                tACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGc                                                  >  2:182127/1‑99 (MQ=255)

                                                  cAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCtt                                                <  1:3308001/99‑1 (MQ=255)

                                                   aGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCttt                                               <  2:1871595/99‑1 (MQ=255)

                                                     cTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTtct                                             >  1:2024152/1‑99 (MQ=255)

                                                       aTTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTctct                                           <  1:2291781/99‑1 (MQ=255)

                                                       aTTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTctct                                           >  2:3304070/1‑99 (MQ=255)

                                                       aTTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTctct                                           <  1:783427/99‑1 (MQ=255)

                                                        ttGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTGTTTTTACGTTTCTTCAGATTGCTCTTCAGCAGCTTCTGCTTTCTCTg                                          >  2:1181177/1‑99 (MQ=255)

                                                          gTAGATAAGTGGGGATACTACGGGTATAAAAAACCCCGCCGGGGCGTGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGaa                                        <  2:415744/99‑1 (MQ=255)

                                                          gTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGaa                                        <  2:3425179/99‑1 (MQ=255)

                                                          gTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGaa                                        >  2:952283/1‑99 (MQ=255)

                                                          gTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGaa                                        <  1:1536103/99‑1 (MQ=255)

                                                             gATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGa                                     <  1:3071792/99‑1 (MQ=255)

                                                             gATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGa                                     >  1:2773516/1‑99 (MQ=255)

                                                              aTAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGAt                                    >  1:1767655/1‑99 (MQ=255)

                                                                aaGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCa                                  <  1:585156/99‑1 (MQ=255)

                                                                  gTGGGGATACTACGGGTATAAAAAAACCCGTCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAAc                                <  2:750360/99‑1 (MQ=255)

                                                                  gTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAAc                                >  2:2484839/1‑99 (MQ=255)

                                                                  gTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTGCTGCTTTCTCTGAACGATCAAc                                >  2:505838/1‑99 (MQ=255)

                                                                  gTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAAATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAAc                                >  2:1046042/1‑99 (MQ=255)

                                                                   tGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAAcc                               >  1:680810/1‑99 (MQ=255)

                                                                    ggggATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCa                              >  1:3200441/1‑99 (MQ=255)

                                                                       gATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCt                           >  1:3333796/1‑99 (MQ=255)

                                                                           ctacGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGAt                       <  2:1684894/99‑1 (MQ=255)

                                                                           ctacGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGAt                       <  2:2356436/99‑1 (MQ=255)

                                                                            tacGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATg                      >  1:1883190/1‑99 (MQ=255)

                                                                              cGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTa                    >  1:3152778/1‑99 (MQ=255)

                                                                                 gTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGc                 >  1:14683/1‑99 (MQ=255)

                                                                                  cgtaAAAAAACCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGcc                >  2:2308093/3‑99 (MQ=38)

                                                                                   gtaAAAAAACCCGCCCCGGCGGGTTCTTTTACGTTGCTCCAGATTACTCTGCAGCAACTTCTGCTTCCTCTAAACGATCAACCAGCTCGATGTAAGCCa               >  2:1894044/2‑99 (MQ=37)

                                                                                    taAAAAAACCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCAt              >  1:1995451/1‑99 (MQ=255)

                                                                                     aaaaaaaCCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATc             >  1:629566/1‑99 (MQ=255)

                                                                                      aaaaaaCCCGCCCCGGCGGCTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCg            >  1:2715722/1‑99 (MQ=39)

                                                                                       aaaaaCCCGTAGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTTTGCTTTCTCTGAACGGACAACCAGCGTGTTGTAAGCCATCgg           >  2:2402518/1‑99 (MQ=255)

                                                                                       aaaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCACCTCGACGTAAGCCATCgg           >  2:2019464/1‑99 (MQ=255)

                                                                                       aaaaaCCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATctg           >  2:1354433/1‑97 (MQ=255)

                                                                                       aaaaaCCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCgg           >  1:1817780/1‑99 (MQ=255)

                                                                                       aaaaaCCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCgg           >  1:1825194/1‑99 (MQ=255)

                                                                                       aaaaaCCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCgg           >  1:2172612/1‑99 (MQ=255)

                                                                                       aaaaaCCCGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTATCTCTGAACGATCAACCAGCTCGATGTAAGCCATCgg           >  2:1778152/1‑99 (MQ=39)

                                                                                        aaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgc          >  2:3091156/1‑99 (MQ=255)

                                                                                        aaaaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgc          >  2:1398377/1‑99 (MQ=255)

                                                                                          aaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGgcgc        <  2:1500199/99‑1 (MQ=255)

                                                                                          aaCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCCCGATGTAAGCCATCGgcgc        <  2:777771/99‑1 (MQ=255)

                                                                                            cccGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGt      <  2:1991586/99‑1 (MQ=255)

                                                                                            cccGCCCCGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGt      >  1:2056722/1‑99 (MQ=255)

                                                                                             ccgccgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGtt     >  2:2458889/1‑99 (MQ=255)

                                                                                             ccgccgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGtt     >  1:2979064/1‑99 (MQ=255)

                                                                                               gccgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGt   >  1:1297340/1‑99 (MQ=255)

                                                                                                ccgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTc  <  2:2609130/99‑1 (MQ=255)

                                                                                                ccgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTc  <  2:2589597/99‑1 (MQ=255)

                                                                                                ccgGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTc  <  2:56569/99‑1 (MQ=255)

CACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTC  >  NC_000913/3439490‑3439684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`