Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 354,812 C→T intergenic (+220/‑110) prpE → / → codB propionate‑‑CoA ligase/cytosine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913354,8120CT99.3% 436.7 / ‑4.7 139intergenic (+220/‑110)prpE/codBpropionate‑‑CoA ligase/cytosine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (60/78);  total (60/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

Only 100 of 147 total aligned reads displayed.

CCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAA  >  NC_000913/354716‑354907
                                                                                                |                                                                                               
ccGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCCGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATtc                                                                                               >  1:21742/1‑99 (MQ=255)
 cGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATtca                                                                                              >  2:190318/1‑99 (MQ=255)
  gACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTtcattcat                                                                                             >  2:2169619/1‑99 (MQ=255)
  gACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATtcat                                                                                             >  1:1014246/1‑99 (MQ=255)
  gACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATtcat                                                                                             >  1:2884153/1‑99 (MQ=255)
  gACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATtcat                                                                                             >  1:2609204/1‑99 (MQ=255)
  gACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATtcat                                                                                             <  2:2986694/99‑1 (MQ=255)
    cacCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCattcattc                                                                                           <  1:110820/99‑1 (MQ=255)
    cacCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCattcattc                                                                                           >  1:2378981/1‑99 (MQ=255)
     acCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCCttcattca                                                                                          >  2:1246705/1‑99 (MQ=255)
     acCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCAttcattca                                                                                          <  1:2741272/99‑1 (MQ=255)
     acCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCACGCCTACCCACTCTTTTTACACCGATACTTTTTCCCCCACCTTTTTGCAttcattca                                                                                          <  1:3025060/99‑1 (MQ=37)
      ccATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATtcattcat                                                                                         <  2:2654389/99‑1 (MQ=255)
       cATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCata                                                                                        >  2:718109/1‑99 (MQ=255)
       cATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCata                                                                                        >  1:2143910/1‑99 (MQ=255)
       cATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCata                                                                                        >  1:2009985/1‑99 (MQ=255)
        aTGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCatat                                                                                       >  2:1989918/1‑99 (MQ=255)
          gCTCAGATGCCTGCTGCGCCGCTGACGCCTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTTCCTTCATTCatatca                                                                                     >  2:3269795/1‑97 (MQ=37)
             cAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATaaaaa                                                                                  <  2:1671225/99‑1 (MQ=255)
               gATGCCTGATGCGACGCTGACGCGTCTAAAAACGCCTATCCACTGTTTTTACACCTATAATTTTTCCCCCACCTTTTTACATTCATTCATATAAAAAat                                                                                <  2:1997837/99‑1 (MQ=37)
                 tGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAatat                                                                              <  2:733462/99‑1 (MQ=255)
                 agCCTGATGCGACGCTGACGCCTCTGATCAGGCCTCGCCACTGTTTTTACACCTATAATTTTTCCCCCACCTTTTTGCATTCATTCATAGAAAAAatat                                                                              <  2:3239956/98‑1 (MQ=37)
                  gCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAatata                                                                             <  1:2053179/99‑1 (MQ=255)
                   ccTGATGCGACGATGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTATTTGCATTCATTCATATAAAAActctat                                                                            >  2:1761569/1‑99 (MQ=38)
                     tGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATAttt                                                                          <  1:550854/99‑1 (MQ=255)
                     tGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATAttt                                                                          >  1:1155658/1‑99 (MQ=255)
                     tGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATAttt                                                                          >  1:112718/1‑99 (MQ=255)
                      gATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTc                                                                         >  1:342712/1‑99 (MQ=255)
                      gATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTc                                                                         >  2:812949/1‑99 (MQ=255)
                      gATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTc                                                                         <  2:2526723/99‑1 (MQ=255)
                       aTGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTcc                                                                        >  1:3026970/1‑99 (MQ=255)
                        tGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATGATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTccc                                                                       <  1:3236301/99‑1 (MQ=255)
                        tGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTccc                                                                       <  2:2844645/99‑1 (MQ=255)
                           gACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCAc                                                                    >  1:2660626/1‑99 (MQ=255)
                               cTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTTTTTTTTTTTTTATAAAAAATACATTTCCCCACGaaa                                                                >  2:2823911/1‑99 (MQ=255)
                               cTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACAGATAATTTTTCCCCCCCCTCTTTGCATTCATTGATATAAAAAATATATTTCCCCAAGaaa                                                                >  2:3273414/1‑99 (MQ=255)
                                tGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGaaaa                                                               <  1:2406029/99‑1 (MQ=255)
                                  aCGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCTTTCTTTTATATAAAAAATATATTTCCCCACGAAAACg                                                             >  2:2186159/1‑99 (MQ=255)
                                   cgcgTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGa                                                            >  1:1483795/1‑99 (MQ=255)
                                    gcgTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGAt                                                           <  1:615076/99‑1 (MQ=255)
                                    gcgTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGAt                                                           >  1:136314/1‑99 (MQ=255)
                                     cgTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGAtt                                                          <  2:676725/99‑1 (MQ=255)
                                         ttATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCAACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCtt                                                      <  1:2112078/99‑1 (MQ=255)
                                          tATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCttt                                                     >  1:1530133/1‑99 (MQ=255)
                                           aTCAGGCCTACCCACTGTGTTTACACCGATAATTTTTCCCCAACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCtttt                                                    <  2:3404328/99‑1 (MQ=255)
                                            tCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCTATGAAAACTATTGCttttt                                                   <  2:920431/99‑1 (MQ=255)
                                               ggCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATc                                                >  1:511134/1‑99 (MQ=255)
                                                 ccTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCtt                                              <  2:268249/99‑1 (MQ=255)
                                                  cTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTc                                             <  1:1034923/99‑1 (MQ=255)
                                                   tACCCACTGTTTTTACACCGATACTTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCa                                            <  2:1808929/99‑1 (MQ=255)
                                                   tACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCa                                            <  1:1129236/99‑1 (MQ=255)
                                                   tACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCa                                            <  1:3080748/99‑1 (MQ=255)
                                                   tACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCa                                            <  1:165664/99‑1 (MQ=255)
                                                     cccACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGa                                          <  1:1281785/99‑1 (MQ=255)
                                                     cccACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGa                                          >  2:1428581/1‑99 (MQ=255)
                                                     cccACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGa                                          <  1:3364706/99‑1 (MQ=255)
                                                      ccACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGAt                                         <  1:2205386/99‑1 (MQ=255)
                                                      ccACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGAt                                         >  2:2375995/1‑99 (MQ=255)
                                                       cACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAGCGATGGCTTTTTATCTTCAGATg                                        >  1:1332752/1‑99 (MQ=255)
                                                        aCTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGa                                       >  1:962306/1‑99 (MQ=255)
                                                         cTGTTTTTACACCGATAATTTTTTTTTTTTTTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGaa                                      >  2:461403/1‑99 (MQ=255)
                                                         cTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGaa                                      <  2:1036881/99‑1 (MQ=255)
                                                           gTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATa                                    <  2:912278/99‑1 (MQ=255)
                                                           gTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATa                                    <  2:2732001/99‑1 (MQ=255)
                                                           gTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATa                                    >  1:1507529/1‑99 (MQ=255)
                                                             ttttACACCGATAATTTTTCCCCCACCTTTTTGTATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGa                                  >  2:1449600/1‑99 (MQ=255)
                                                              tttACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGaa                                 <  1:2561179/99‑1 (MQ=255)
                                                              tttACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGaa                                 <  1:2561226/99‑1 (MQ=255)
                                                               ttACACCGATTATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAAt                                <  1:1925442/99‑1 (MQ=255)
                                                                tACACCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATg                               <  2:1568662/99‑1 (MQ=255)
                                                                  cacCGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATgcg                             >  1:916104/1‑99 (MQ=255)
                                                                   acCGATAATTTTTCCCCCCCCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATgcgg                            <  1:1480476/99‑1 (MQ=255)
                                                                   acCGATAATTTGTCCCCCACCGTTTTGCATTCATTCAGATAAAAAATATATTTCCCCACGAAAACGATTTCTGTTTATCTTCAGATGAATAGAATgcga                            >  2:685068/1‑98 (MQ=255)
                                                                    ccGATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATgcggc                           <  2:2292209/99‑1 (MQ=255)
                                                                      gATAATTTTTCCGCAACAATTTTGGATTCTTTTATATAAAAACTATATTTCTCCCCGACAACGATTGCTTTTTATCTTCCGATGACTAGAATGtggcgg                         >  2:242455/1‑99 (MQ=255)
                                                                      gATAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGcggcgg                         >  2:3179693/1‑99 (MQ=255)
                                                                       aTAATTTTTCCCCCACCTTTTTTTTTTTTTTTTTATAAAAAATATATTTCCCCACGAATATGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGa                        >  2:3356342/1‑99 (MQ=255)
                                                                       aTAATTTTTCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGa                        >  2:1628578/1‑99 (MQ=255)
                                                                           tttttCCCCCACCTTTTTTCATTCTTTTATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATATAATGCGGCGGAtttt                    >  2:1850082/1‑99 (MQ=255)
                                                                           tttttCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGAtttt                    >  1:201725/1‑99 (MQ=255)
                                                                           tttttCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGAtttt                    >  1:2571603/1‑99 (MQ=255)
                                                                            ttttCCCCCCCCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGAttttt                   <  1:646480/99‑1 (MQ=255)
                                                                            ttttCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGAttttt                   <  1:1290387/99‑1 (MQ=255)
                                                                            ttttCCCACACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGAttttt                   <  2:1851407/99‑1 (MQ=255)
                                                                             tttCCCCCACCTTTTTGCATTCATTCATATAACAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGAtttttt                  <  2:2067043/99‑1 (MQ=255)
                                                                             tttCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGAtttttt                  <  2:807223/99‑1 (MQ=255)
                                                                             tttCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGAtttttt                  <  2:1201559/99‑1 (MQ=255)
                                                                              ttCCCCCACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTg                 <  2:2442634/99‑1 (MQ=255)
                                                                               tCCCCCACCTTTTTGCATTCATTCATATAAAGAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATATAATGCGGCGGATTTTTTgg                <  1:1808528/99‑1 (MQ=255)
                                                                                cccccACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGAttttttttt               >  2:1964019/1‑96 (MQ=255)
                                                                                cccccACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTggg               <  1:209593/99‑1 (MQ=255)
                                                                                   ccACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGttt            >  1:1444085/1‑99 (MQ=255)
                                                                                   ccACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGttt            >  1:3256380/1‑99 (MQ=255)
                                                                                   ccACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGttt            >  2:1607001/1‑99 (MQ=255)
                                                                                    cACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTc           <  1:3180884/99‑1 (MQ=255)
                                                                                    cACCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTc           <  1:2799649/99‑1 (MQ=255)
                                                                                     aCCTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCa          <  2:358352/99‑1 (MQ=255)
                                                                                       cTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCaaa        <  2:2732229/99‑1 (MQ=255)
                                                                                       cTTTTTGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCaaa        <  2:2522002/99‑1 (MQ=255)
                                                                                           ttGCATTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGc    <  1:1172078/99‑1 (MQ=255)
                                                                                                |                                                                                               
CCGACACCATGCTCAGATGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCCACTGTTTTTACACCGATAATTTTTCCCCCACCTTTTTGCACTCATTCATATAAAAAATATATTTCCCCACGAAAACGATTGCTTTTTATCTTCAGATGAATAGAATGCGGCGGATTTTTTGGGTTTCAAACAGCAA  >  NC_000913/354716‑354907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: