Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 644,734 G→T A90A (GCC→GCA)  rna ← ribonuclease I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913644,7340GT84.5% 253.1 / 48.2 123A90A (GCC→GCA) rnaribonuclease I
Reads supporting (aligned to +/- strand):  ref base G (8/11);  new base T (58/46);  total (66/57)
Fisher's exact test for biased strand distribution p-value = 3.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

Only 100 of 123 total aligned reads displayed.

CGATGAACACATTCGGCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGGGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGT  >  NC_000913/644638‑644830
                                                                                                |                                                                                                
cGATGAACACATTCGGCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGGgc                                                                                                <  2:2722643/99‑1 (MQ=255)
cGATGAACACATTCGGCTGGCGCGCGCTCCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTgc                                                                                                <  2:746509/99‑1 (MQ=255)
 gATGAACACATTCGGCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTgca                                                                                               <  2:1484598/99‑1 (MQ=255)
 gATGAACACATTCGGCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACAACGGgca                                                                                               <  1:1407990/99‑1 (MQ=255)
     aaCACATTCGGCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCaa                                                                                           >  2:1378322/1‑99 (MQ=255)
      acacATTCGGCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAAc                                                                                          <  1:2871204/99‑1 (MQ=255)
        acaTTCGGCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCg                                                                                        >  1:1230005/1‑99 (MQ=255)
         caTTCGGCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGa                                                                                       <  1:2944066/99‑1 (MQ=255)
          aTTCGGCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGAt                                                                                      >  1:1038984/1‑99 (MQ=255)
          aTTCGGCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGAt                                                                                      <  2:1860002/99‑1 (MQ=255)
          aTTCGGCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGAt                                                                                      >  1:900420/1‑99 (MQ=255)
          aTTCGGCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGAt                                                                                      >  1:221738/1‑99 (MQ=255)
          aTTCGGCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGGGCAGCAACCGAt                                                                                      >  2:1215945/1‑99 (MQ=255)
          aTTCGGCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCACATCCAGCGGCGTTCATCAACACCACGGGCAGCAACCGAt                                                                                      <  1:684514/99‑1 (MQ=255)
          aTTCGGCTGG‑ATGCGCGTCTGGTAGATTCGGGATTGGGGGAGTAGCGCAACCGAAGGGCATCCAGCGGCGGTTATCAACACCACGTGCAGCAACCGAtt                                                                                     >  2:686402/1‑99 (MQ=255)
           ttCGGCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGAtt                                                                                     >  2:397697/1‑99 (MQ=255)
             cGGCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTa                                                                                   <  1:2622782/99‑1 (MQ=255)
              ggCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAg                                                                                  <  1:1944594/99‑1 (MQ=255)
              ggCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAg                                                                                  <  2:1957339/99‑1 (MQ=255)
              ggCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAg                                                                                  <  1:263046/99‑1 (MQ=255)
               gCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAgg                                                                                 <  1:95813/99‑1 (MQ=255)
                 tGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTGAGGCa                                                                               >  1:3292631/1‑99 (MQ=255)
                       gcgcTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTg                                                                         >  2:1167471/1‑99 (MQ=255)
                         gcTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGGGCAGCAACCGATTTAGGCAATCCTGGc                                                                       <  1:542389/99‑1 (MQ=255)
                         gcTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGGGCAGCAACCGATTTAGGCAATCCTGGc                                                                       <  2:2024751/99‑1 (MQ=255)
                           ttgtGGTATATTCGGTATTGGGCAAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACTTGCAGCAACCGATTTAGGCAATCCTGGCca                                                                     <  2:2812752/96‑1 (MQ=255)
                             cTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCcaca                                                                   <  1:2710730/99‑1 (MQ=255)
                             cTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCcaca                                                                   <  1:1267431/99‑1 (MQ=255)
                               ggTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGa                                                                 >  1:3304628/1‑99 (MQ=255)
                               ggTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGAATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGa                                                                 >  2:351916/1‑99 (MQ=255)
                                gTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGAc                                                                >  1:631128/1‑99 (MQ=255)
                                gTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGAc                                                                >  2:344971/1‑99 (MQ=255)
                                gTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAAACGATTTAGGCAATCCTGGCCACAGAc                                                                >  2:2394615/1‑99 (MQ=255)
                                   gATTCGGGAGTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCAt                                                             >  2:3155796/1‑99 (MQ=255)
                                   gATTCGGGACTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCGACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCAt                                                             >  2:3155822/1‑99 (MQ=255)
                                      tCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCCTCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTa                                                          >  2:1151915/1‑99 (MQ=255)
                                      tCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTa                                                          >  2:1347226/1‑99 (MQ=255)
                                        gggATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCATGGGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACg                                                        >  1:1576599/1‑99 (MQ=255)
                                        gggATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACg                                                        >  1:1604477/1‑99 (MQ=255)
                                        gggAATGGGCGAGGAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTTCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACg                                                        >  2:2254111/1‑99 (MQ=255)
                                          gATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGt                                                      >  1:2101475/1‑99 (MQ=255)
                                              gggCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGa                                                  >  1:1273019/1‑99 (MQ=255)
                                                gCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGaaa                                                >  2:759097/1‑99 (MQ=255)
                                                gCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGaaa                                                >  1:2340798/1‑99 (MQ=255)
                                                gCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGaaa                                                >  1:278953/1‑99 (MQ=255)
                                                  gAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAAt                                              <  1:2129165/99‑1 (MQ=255)
                                                  gAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAAt                                              <  1:954303/99‑1 (MQ=255)
                                                  gAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAAt                                              >  1:1464362/1‑99 (MQ=255)
                                                    gTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCa                                            <  1:3340884/99‑1 (MQ=255)
                                                      aGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGGGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGc                                          <  1:754634/99‑1 (MQ=255)
                                                       gcgcAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCt                                         <  1:2213702/99‑1 (MQ=255)
                                                        cgcAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGGGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCtt                                        >  2:276753/1‑99 (MQ=255)
                                                         gcAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCttt                                       <  2:2189078/99‑1 (MQ=255)
                                                           aaCCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTttgt                                     <  2:3051368/99‑1 (MQ=255)
                                                            aCCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTttgtt                                    <  1:3401526/99‑1 (MQ=255)
                                                            aCCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTttgtt                                    <  1:2987377/99‑1 (MQ=255)
                                                            aCCGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTttgtt                                    <  2:2470357/99‑1 (MQ=255)
                                                             ccGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTttgttg                                   >  2:1568537/1‑99 (MQ=255)
                                                              cGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTgg                                  >  1:2494464/1‑99 (MQ=255)
                                                              cGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTgg                                  >  1:681255/1‑99 (MQ=255)
                                                              cGAAGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTgg                                  >  2:1880294/1‑99 (MQ=255)
                                                                aaGCGCATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTc                                <  1:835730/99‑1 (MQ=255)
                                                                  gcgcATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGt                              >  2:2048045/1‑99 (MQ=255)
                                                                    gcATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGttt                            <  2:609786/99‑1 (MQ=255)
                                                                    gcATCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGttt                            >  2:2581390/1‑99 (MQ=255)
                                                                       tCCAGCGGCGTTCTTCAACACCACGTGCAGCTACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCgg                         <  2:2683687/99‑1 (MQ=255)
                                                                       tCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCgg                         >  2:2615589/1‑99 (MQ=255)
                                                                       tCCAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCgg                         <  1:1389237/99‑1 (MQ=255)
                                                                        ccAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGt                        >  1:3042581/1‑99 (MQ=255)
                                                                        ccAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGt                        >  1:1004675/1‑99 (MQ=255)
                                                                        ccAGCGGCGTTCATCAACACCACGGGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGt                        <  2:2910831/99‑1 (MQ=255)
                                                                         cAGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGtt                       >  1:1072086/1‑99 (MQ=255)
                                                                          aGCGGCGTTCATCAACCCCACATGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGttt                      <  2:2470927/99‑1 (MQ=255)
                                                                          aGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGttt                      >  2:1457240/1‑99 (MQ=255)
                                                                          aGCGGCGTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGGTGGTCGTTTCGGttt                      >  1:2285325/1‑99 (MQ=255)
                                                                           gcggcgTTCATCAACACCACGGGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTg                     <  2:1129357/99‑1 (MQ=255)
                                                                            cggcgTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGc                    <  2:2093870/99‑1 (MQ=255)
                                                                              gcgTTCATCAACACCACGGGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAg                  >  2:2194693/1‑99 (MQ=255)
                                                                              gcgTTCATCAACACCACGGGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAg                  >  1:561857/1‑99 (MQ=255)
                                                                               cgTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAgg                 <  2:220890/99‑1 (MQ=255)
                                                                                gTTCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGc                <  1:656965/99‑1 (MQ=255)
                                                                                 ttCATCAACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCg               >  1:499047/1‑99 (MQ=255)
                                                                                 ttCATCAACACCACGGGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCg               >  1:2299103/1‑99 (MQ=255)
                                                                                    atcaACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACa            <  2:219084/99‑1 (MQ=255)
                                                                                    atcaACACCACGTGCAGCAACCGATTTAGGCAATACTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACa            >  1:3359657/1‑99 (MQ=255)
                                                                                     tcaACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACAt           >  2:1627210/1‑99 (MQ=255)
                                                                                     tcaACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGCTCAGAAAATCAGCTTTGTTGGTCGTTTCGCTCTTCAGTCGACac           >  2:1346529/1‑98 (MQ=255)
                                                                                      caACACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACAtt          <  1:1167912/99‑1 (MQ=255)
                                                                                       aaCACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTc         <  2:275181/99‑1 (MQ=255)
                                                                                       aaCACCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTc         <  2:438419/99‑1 (MQ=255)
                                                                                        acacCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCa        >  1:2278037/1‑99 (MQ=255)
                                                                                        acacCACGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCa        <  1:349935/99‑1 (MQ=255)
                                                                                          accacGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCTGTTTGCAGGCGACATTCAtc      <  1:2588027/99‑1 (MQ=255)
                                                                                          accacGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCAtc      >  1:2542941/1‑99 (MQ=255)
                                                                                          accacGGGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCAtc      <  1:304552/99‑1 (MQ=255)
                                                                                           ccacGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCAtct     >  1:386952/1‑99 (MQ=255)
                                                                                           ccacGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAAGTAGCTTGGTTGGTAGTTTCGGTTTGCAGGCGACATTCAtct     >  2:3271742/1‑99 (MQ=255)
                                                                                           ccacGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGGACGGTCATAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCAtct     >  2:2428453/1‑99 (MQ=255)
                                                                                            cacGTGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCAtctc    >  1:1718924/1‑99 (MQ=255)
                                                                                              cGGGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCtcgt  <  2:274678/99‑1 (MQ=255)
                                                                                                |                                                                                                
CGATGAACACATTCGGCTGGCGCGCGCTTCTGGTAGATTCGGGATTGGGCGAGTAGCGCAACCGAAGCGCATCCAGCGGCGTTCATCAACACCACGGGCAGCAACCGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGT  >  NC_000913/644638‑644830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑