Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,745,3970AC39.7% 105.2 / 15.3 82L198F (TTA→TTCyiaN2,3‑diketo‑L‑gulonate TRAP transporter large permease protein
Reads supporting (aligned to +/- strand):  ref base A (31/16);  new base C (0/31);  total (35/47)
Fisher's exact test for biased strand distribution p-value = 3.32e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTGATTGCCTCTGGCGGCATTATCGCGCCAATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAG  >  NC_000913/3745166‑3745632
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            
aCTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTGATTGCCTCTGGCGGCATTATCGCGCCAATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGTCCATTGCCCCTGCATGATGATTGGCGGCACGCTGTAACTTATCTGGTGGTTGGGGTGCCGTCGCCCTCATTTAACCCAcc                                                                                                                                                                                                                                       >  2:139031/1‑239 (MQ=255)
       ccGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTGATTGCCTCTGGCGGCATTATCGCGCCAATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCCTGGTGATTGGCGCAACGCTGTGGCTTACCTGGTGGTGGCACGCCGGTCGCCtttattta                                                                                                                                                                                                                                              >  2:466330/1‑225 (MQ=255)
              gggccAGCCCCAAGTAACCGGTCAGCCGGGGGGCGGGGTGTATTGCCTCTGGCGGCTTTACCGGCCCAATTTTTCCCCCTTCCATTCCTTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTTTTTAGGGCGGGCTTTGCCCCTGGCATGATGATGGGCGCACCGCTGATGCTTACCTGGGGGGGGCGGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAAc                                                                                                                                                                                                                          <  2:1463861/234‑1 (MQ=255)
               gcgcAGCGCCACCTTTCCGGTCAACCGGGCGGCGGGGCTGATTGCCTCTGGCGGCATTATCGCGCCAATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGAATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGa                                                                                                                                                                                                                        <  1:859629/239‑1 (MQ=255)
                     cgcCAACTATCCGGTCAACCGGGCGGCGGGGCTGATTGCCTCTGGCGGCATTATCGCGCCAATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGGGCAACGCTTATGGTTACCTGGGTGTGGCAGGCCCGTCGCCTTAATTTTCCCCGCCCGCAAAAAGCAACGAGGCAgg                                                                                                                                                                                                                  >  1:205631/1‑239 (MQ=255)
                               ccGGTCAACCGGGCGGCGGGGCTGATTGCCTCTGGCGGCATTATCGCGCCAATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCCGGGATGATGATGGTCGCCACGCCGATGCTTACCTGGGGGTGGGCGGCCAGGCGCCTTTTTTTACCCCGCCCGCAAAAAGCAACCCTGCAGGGAAACttgg                                                                                                                                                                                                         >  2:1817349/1‑235 (MQ=255)
                                  gTCAACCGGGCGGCGGGGCTGATTGCCTCTGGCGGCATTATCGCGCCAATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCGGCATTTTGATGGGCGGAACGCCTATGCTTACCTGGTGGTGGGGGGCCAGACGCCTTAATTTACCCCCCCCGCACAAATAAAAGATGCAGCAAATCTTGc                                                                                                                                                                                                         >  2:830006/1‑235 (MQ=255)
                                         gggCGGCGGGGCTGATTGCCTCTGGCGGCATTATCGCGCCAATTATTCCCCCTTCCATTCCTTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTAGGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGtc                                                                                                                                                                                              <  1:2063247/239‑1 (MQ=255)
                                            cggcggGGTTGATTGCCTCTGGCGGCATTATCGCGCCAATTATTCCCCCTTCCATTCCGTTTTTTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGtctc                                                                                                                                                                                            <  1:1817349/238‑1 (MQ=255)
                                            cgcggggtCTGATTCCTTCGGGCGGCATTGTCGCCCCATTTATTCCCCTTCCCATCCCGTTTATTATCTACGGCGTTGCCAGGGGATTATCCATCAGAAAGCTGTTTATGGCGGGCATTGCCCCTGGAATGATGATGGGCGCAACGCTGAGGCTAACCGGGTGGGGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGAACTCTTTTGtctc                                                                                                                                                                                            <  2:2038057/234‑1 (MQ=255)
                                               cggGGCTGATTGCCTCTGGCGGCATTATCGCGCCAATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGCTTTCCCCCGTCTTGATGGCTGGCGCCACGCTCTTCCTTTCCTGGGGGTGGCAGGCCCGGCGACCTAACTTACCCCCCCAGCAAAAAGCAACGATTCAGGCACTCTGGCACTCTTTTGTCTCCggg                                                                                                                                                                                        >  2:1595902/1‑238 (MQ=255)
                                                                   ggcATTATCGCCCCAATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTCCCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttcttc                                                                                                                                                                     <  1:2226578/238‑1 (MQ=255)
                                                                     ctttACCGCGCCATTTATTCCCCCTTCCATCCCGTTTATTTTCTCCGGGTTTTCCAGCGGATTATCCATCATTAAGCTTTTTATGGCGGGCATTGCCCTTGGCATGATGTTGGGCGCACCGCTGTTGCTTCCCTGGTGGTGGCAGGCCGGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCa                                                                                                                                                                   <  2:1752615/236‑1 (MQ=255)
                                                                     cagtATCGCGCCAATTATTCCCCCTTCCTTTCCGTTTATTATCTTCGGCGTTGCCAGCGGATTATCCATCGGTAAGCTGTTTAGGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCGGGAAATCTGGCACTCtt                                                                                                                                                                                                   <  1:1933778/203‑1 (MQ=255)
                                                                      aTTATCGCGCCAATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAg                                                                                                                                                                  <  1:52867/238‑1 (MQ=255)
                                                                               ccAATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGGCTGATGATGGGGGCAAAGCTGTTGCTTTCCTGGGGGGGGGCGGGCAGGCGCCTTAATTTATCCCGCCCGCAAAAAAAAACGAGGCGGGAGATCTGGGACTCTTTTTTTTCCgg                                                                                                                                                                                         >  2:1933778/1‑206 (MQ=255)
                                                                                 atttattCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCGTCAGTAAGCTGTTTAGGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCATTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTg                                                                                                                                                      <  1:1790760/237‑1 (MQ=255)
                                                                                        cccccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGtt                                                                                                                                               <  1:212579/239‑1 (MQ=255)
                                                                                             ttCCATTCCGTTTATTTTCTTCGGCTTTACCAGCGGATTATCCATCAATAAGCGGTTTATGGCGGGCATTGCCCATGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCt                                                                                                                                         <  1:1301505/239‑1 (MQ=255)
                                                                                             ttCCATTCCGTTTATTATCTTCGGCCTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCACCGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCaaaa                                                                                                                                                                                                                                <  1:1200184/153‑1 (MQ=255)
                                                                                              tCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCCTGATGATGGGCGCCACGCTTATGCTTACCTGGTGGGGGCAGGCCAGTCGCCCTAATTTTCCCCCCCCGCAAAAAGCAACCGTGCAGGAAATCTGTCACTCTTTTGTCTCCGGGATCTGGGCCCCGTTTTTTCCCATCTTCCTTATTTGCgggg                                                                                                                                                >  2:837106/1‑231 (MQ=255)
                                                                                                    ccttttATTATTTTCGGCCTTTCCGGCGGATTACCCATCAGTAAGCTGTTTATGGCGGGCTTTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCTTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGc                                                                                                                                    <  1:419683/234‑1 (MQ=255)
                                                                                                       tttATTATCTTCGGCTTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCGGTCCCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgcg                                                                                                                                                                               <  1:875763/192‑1 (MQ=255)
                                                                                                       tttATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGGGGCAGGGCAGTCGCCTTAATTTTCCCCCCCCGCCAAAAGCAACGaatc                                                                                                                                                                                                                     >  2:1200184/1‑151 (MQ=255)
                                                                                                        ttattaTCTTCGGCTTTTCCAGGGGATTACCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGGGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGtt                                                                                                                               <  1:1543060/238‑1 (MQ=255)
                                                                                                                 tCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCGGCATGATGATGGGGGGAAAGCTGATGCTTACCTTGTGGTGGCCGGCCAGGCGCCCTAATTTTCCCCGCCCGCAAAAAACAACGATGCAGGAAAACCTGCACTCTTTTTTCTCCCGATTCCGTGCGCTGTTTTTTCCCGTCTTCATTATTGGGGCGTTGCTCTTCCGGCCGATTTTCTCCTAc                                                                                                                      >  2:953192/1‑239 (MQ=255)
                                                                                                                 tCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCCGGCATGATGATGGGCGCAAAGCTGATGCTTACCTGGTGGGGGGGGGGCCGTTGCCTTTATTTACCCCCGCCGCAAAAAACCACGATGGAGGGAATCCGGCACTCTTTTTTTCCCCGGATTTGGGGGCTGttttctc                                                                                                                                                                     >  2:875763/1‑188 (MQ=255)
                                                                                                                   ggCTTTTCCAGCGGATTATCCATCAGTAAGCTGTTTTTGGCGGGCATTCCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCGGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGTCAGCa                                                                                                                                                                                                                                   <  2:1618036/128‑1 (MQ=255)
                                                                                                                             gCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCCTTTCCCCCTGCATGATGTTGGGCGCAACGCTGATGCTTACCTTGGGGGGGCAGGCCACTCGCCTTAATTTACCCCGCCCACAAAAAGCaaag                                                                                                                                                                                                                         >  1:1618036/1‑126 (MQ=255)
                                                                                                                                 aTTATCCATCAGTAAGCTGTTTATGGCGGGCAATGCCCCCGGGATGGTGGTTGGGGCAACGCTGATGCTTACCTGGGGGGGGGAGGCCAGTCGCCTTAATTTACCCCGCCCGCCAAAAGCAACGATGCAGGAAAACTTGGACTCTTTTTTCTCCGGGATCTGGgc                                                                                                                                                                                >  2:1181255/1‑165 (MQ=255)
                                                                                                                                    atcaatcaTTAAGCTGTTTATGGGGGGCATGGCCCCTGGCATGATGATGGGCGCAACGCGGATGCTTACCGGGTGGTGGCAGGCCATTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTtgctg                                                                                                  <  1:139031/239‑1 (MQ=255)
                                                                                                                                          cAGTAGGCGGTGTATGGCGGGCGTTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCggg                                                                                                            <  1:2179217/223‑1 (MQ=255)
                                                                                                                                          aaGTAGGCTGTTTAGGGCGGGCATGGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTg                                                                                                                                                                                                <  1:1904955/139‑1 (MQ=255)
                                                                                                                                                    tttATGGCGGGCATTTCCCCCGGCATGATGATGGGCGCAACGCTGATGCTTACCCGGGGGGGGCAGGCCAGTCGCCTTTATTTACCCCGCCAGCAAAAAACAACGGTGCAGGAAAACTGGCACTCTTTTGTCTCCGGGAt                                                                                                                                                                                      >  2:1904955/1‑140 (MQ=255)
                                                                                                                                                     ttAGGGCGGGCATTCCCCCTGGACAGATGGTGGGCGCCACGCTGAGGCTTCCCTGGTGGTGGCAGGCCAGTCGCCTTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGtt                                                                                  <  1:1534762/238‑1 (MQ=255)
                                                                                                                                                       agggCGGGCATGCCCCCTGGCATGATGTTGGGCGCAACGCTGATGCTTACCGGGTGGTGGCAGGCCAGTCGCTTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGt                                                                               <  1:20798/237‑1 (MQ=255)
                                                                                                                                                           cGGGCATTGCCCCTGGCATGAGGATGGGCGCAACGCTGAGGCTTACCTGGTGGGGGCAGGCCAGTCCCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGc                                                                            <  1:1018048/238‑1 (MQ=255)
                                                                                                                                                             ggCTTTGCCCGTGGGATTGTGGTGGGCGCAACGCGGTTGCTTCCCTGGGGGTGGCAGGCCAGTCGCTTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCca                                                                          <  1:469940/238‑1 (MQ=255)
                                                                                                                                                             ggCATTGCCCCTGGCATGATGAGGGGCGCAACGCTGATGCTTCCCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCca                                                                          <  1:2003039/238‑1 (MQ=255)
                                                                                                                                                             ggCATTGCCCCTGGCATGATGAGGGGCGAAACGCTGATGCTTCCCGGGTGGTGGCAGGCCGGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCca                                                                          <  1:465360/238‑1 (MQ=255)
                                                                                                                                                                    gCCCCGGGCTTGTTGAGGGGCGCAACTCTGATGTTTACCGGGTGGTGGCAGGCCTGTCGTCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCca                                                                          <  1:1955512/232‑1 (MQ=255)
                                                                                                                                                                          ggCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  2:735548/1‑125 (MQ=255)
                                                                                                                                                                           gCATGATGAGGGGCACACCGCTGATGCTTACCGGGTGGTGGCGGGCCAGTCGCCTTATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCg                                                             <  2:205631/238‑1 (MQ=255)
                                                                                                                                                                                 tgatgGGCGCAACGCTGATGCTTACCTGGTGGGGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTtgctgc                                                                                                 <  1:879499/196‑1 (MQ=255)
                                                                                                                                                                                  gatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  1:1289503/1‑117 (MQ=255)
                                                                                                                                                                                     gggCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGa                                                                                                                                                                                                                 >  2:513987/1‑81 (MQ=255)
                                                                                                                                                                                       gcgcAACGCTGATGCTTACCTGGGGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  1:1911354/1‑112 (MQ=255)
                                                                                                                                                                                          cAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCaaaa                                                                                                                                                                                                                                >  2:1393292/1‑61 (MQ=255)
                                                                                                                                                                                          cAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCaaaa                                                                                                                                                                                                                                <  1:1393292/61‑1 (MQ=255)
                                                                                                                                                                                          cAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  1:1493454/1‑109 (MQ=255)
                                                                                                                                                                                           aaCGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  2:879499/1‑108 (MQ=255)
                                                                                                                                                                                             cGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  1:1820594/1‑106 (MQ=255)
                                                                                                                                                                                              gCTGATGCTTACCTGGTGGGGGCAGGCCAGTCGCTTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTtg                                                                                                     <  2:2062524/179‑1 (MQ=255)
                                                                                                                                                                                               ttGATGCTTCCCTGGTGGGGGCAGCCCGGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACt                                        <  1:1036660/238‑1 (MQ=255)
                                                                                                                                                                                               cTGATGCTGACCGGTGGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACt                                        <  2:1820594/239‑1 (MQ=255)
                                                                                                                                                                                                 ggtgtttcccTGGGGGGGGCAGGCCGGTCCCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACttt                                      <  1:273637/231‑1 (MQ=255)
                                                                                                                                                                                                 gATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  1:1273034/1‑102 (MQ=255)
                                                                                                                                                                                                  aTGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAAATTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  2:1154979/1‑101 (MQ=255)
                                                                                                                                                                                                  aTGCTTACCTGATGGTGGAAGCCCTGTACCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACt                                        <  2:1514912/236‑1 (MQ=255)
                                                                                                                                                                                                    gCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGc                                   <  1:735548/239‑1 (MQ=255)
                                                                                                                                                                                                        aCCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  1:2062524/1‑95 (MQ=255)
                                                                                                                                                                                                            ggtggtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  1:1422122/1‑91 (MQ=255)
                                                                                                                                                                                                            ggtggtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  1:570660/1‑91 (MQ=255)
                                                                                                                                                                                                            ggtggtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  1:1514912/1‑91 (MQ=255)
                                                                                                                                                                                                            ggtggtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  2:441024/1‑91 (MQ=255)
                                                                                                                                                                                                             gtggtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  1:301488/1‑90 (MQ=255)
                                                                                                                                                                                                             gtggtggAAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTa                              <  1:1545163/235‑1 (MQ=255)
                                                                                                                                                                                                              tggtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  1:762006/1‑89 (MQ=255)
                                                                                                                                                                                                                gtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  1:772372/1‑87 (MQ=255)
                                                                                                                                                                                                                    cAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGCTGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  2:241414/1‑83 (MQ=255)
                                                                                                                                                                                                                        ccAGTCGCTTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCca                                                                          <  1:1637576/180‑1 (MQ=255)
                                                                                                                                                                                                                        ccAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGgcg                    <  1:837106/234‑1 (MQ=255)
                                                                                                                                                                                                                        ccAGTCCCTTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGgcg                    <  1:1181255/234‑1 (MQ=255)
                                                                                                                                                                                                                           gTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGaaa                                                                                                                                                                                                               >  2:364744/1‑45 (MQ=255)
                                                                                                                                                                                                                           gTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  1:1599269/1‑76 (MQ=255)
                                                                                                                                                                                                                            tCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGt                                                                                                                                                                 <  2:301488/90‑1 (MQ=255)
                                                                                                                                                                                                                             cGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGaa             <  1:728405/236‑1 (MQ=255)
                                                                                                                                                                                                                             cGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAac          <  1:547439/239‑1 (MQ=255)
                                                                                                                                                                                                                              gCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACt                                        <  1:1708750/208‑1 (MQ=255)
                                                                                                                                                                                                                                  tAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  2:1637576/1‑69 (MQ=255)
                                                                                                                                                                                                                                   aaTTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCa   <  1:1322816/239‑1 (MQ=255)
                                                                                                                                                                                                                                    aTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCa   <  1:1595902/239‑1 (MQ=255)
                                                                                                                                                                                                                                     tttCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAg  <  1:1659678/239‑1 (MQ=255)
                                                                                                                                                                                                                                        aCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                                                                                                                >  2:1708750/1‑63 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            
ACTGGTGCCGATGATGCGCAGCGCCAACTATCCGGTCAACCGGGCGGCGGGGCTGATTGCCTCTGGCGGCATTATCGCGCCAATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAG  >  NC_000913/3745166‑3745632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: