Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,782,9170GA30.0% 47.4 / 12.4 41L249L (CTG→TTG) gpsAglycerol‑3‑phosphate dehydrogenase (NAD+)
Reads supporting (aligned to +/- strand):  ref base G (6/22);  new base A (11/1);  total (18/23)
Fisher's exact test for biased strand distribution p-value = 5.21e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATA‑CCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCA  >  NC_000913/3782682‑3783150
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          
aaaGTCAATGCTGCCTCGCGCACGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGa                                                                                                                                                                                                                                           <  2:1627929/237‑1 (MQ=255)
    tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCCACTGCGTGTCGGTACAGGGAAGCACCAAATCGCCa                                                                                                                                                                                                                                     >  1:842715/1‑239 (MQ=255)
    tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑CTACCCGACTGGTTGTCGGTCCAGGGAAGCACCAAATCGccc                                                                                                                                                                                                                                     >  1:1805951/1‑238 (MQ=255)
        tgctgcCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTTCCCGCCTGGTTGTCGGGACAGGTCAGCACCAAAACGCCaacc                                                                                                                                                                                                                                  >  1:484153/1‑236 (MQ=255)
             ccTCGCGCGGTTTTTTTCCGCAATAAAATATTTGATAAATTCCCTCGTTTATTGGCATTCCAACGCCGAGGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTCCCCCCCCCTGACCAATCTTCTCCTGCGCGCTTGGTACATCCAGGCCCTGACCGAGCATCATGCCAAACCGGCGT‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGcc                                                                                                                                                                                                                            <  2:842715/239‑1 (MQ=255)
             ccTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGGCTGGTTGTCGGGACAAGGAAGCACCAAAACGCCCAAcccccc                                                                                                                                                                                                                             >  1:1371845/1‑236 (MQ=255)
                                gaaaTATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCACCGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGTTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAg                                                                                                                                                                                                         <  2:2296214/237‑1 (MQ=255)
                                              gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGCCTCGTTGTTGGGACAGGTAAGGACCAAAACGCCAAGCCCCGCCCATCCCCTAAAAGTGGCAGGGTCGGGAccccc                                                                                                                                                                                            >  1:1444311/1‑236 (MQ=255)
                                                                        acAACGCCGAGCCGAGCCGCCCGTTCGCGTACTTCTTTCGTATTGCGGTAGCCTGCCACCACCTGCCCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGGGCATCATGCCAAAACGGCGG‑TTGCGCGACTGGTGGTCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTcagc                                                                                                                                                                 <  2:1805951/238‑1 (MQ=255)
                                                                                     gatgacccAGTCCGTGGCCTTCTTTCCTATTGCGGGAGCCACCCCCCCCCTTACCATTCGTCCCCGCGGTCCTTTGTACAGCCAGCGCCCGCCCGATCTTCATCCAAAACCGGCGG‑TTACGGGACTGGGTGTCGTTACAGGTAAGCACCCGATCCCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACACAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACgg                                                                                                                                                       <  2:1397833/230‑1 (MQ=255)
                                                                                       tGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTATCCGACTGGTTGTCGGGACAAGTAAGCACCAAATCGCCCACCCCCCCCACTCCCCTAAAAGTGGGAGCGGCGGCACCCACCGCCGCCCCCCCACGCGGCAATTCCACCCGCCCACgcggga                                                                                                                                                   >  2:711782/1‑233 (MQ=255)
                                                                                                     gtctgcttTCGTATGGCGGCAGCCTCCCACCACCGGCCCAATCTCCCCCTGCCGTCTTGTTCCAACCATCCCCTGCCCGAGCCTCAGCACAAACCGGCGG‑TTCGCCGCCTGGTGGTCGGTACGGGTAAGCACCAGACCGCCACGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCCCGGGTGATCAGCGCCGTAcgc                                                                                                                                     <  2:1423659/233‑1 (MQ=255)
                                                                                                         tcttTCGTATTGCGGTAGCCTTCCCCCCCCTGACCAATCTTCTCCTGCGCGCTTTTTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTCCGCGACTGTTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCAtt                                                                                                                                <  1:1590767/239‑1 (MQ=255)
                                                                                                           tttCGTATTGGGGTAGCATCCCACCACCTGACCAATCTTCTCCTGCGCCCTTTGTACATCCATGCCCTGCCCGAGCATCAGCCCAAACCGGGGG‑TTACGCGACGGGTTGTCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGCCAt                                                                                                                                                                                                                          <  2:1529610/147‑1 (MQ=255)
                                                                                                               gttttGCGGTAGCCTTCCACCCCCGGACCAATCTTCTCCGGCGCGCTTTGTACATCCATGCCCTGCCCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCAcc                                                                                                                          <  1:2238307/236‑1 (MQ=255)
                                                                                                                     cGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTAAGCGACTTGTTGGTGGGACAGGGAAGCACCAGATCACCAAACCCCGCCATTTCCATaaaa                                                                                                                                                                                                                >  1:1529610/1‑146 (MQ=255)
                                                                                                                            cTTCCCCCCCCTGACCAATCTTCTCCGGCGCGTTTTGTACATCCATGCCTTGCCCGAGCATCAGGCCAAAACGGCGG‑TTACGCGACTGTTTGCCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATA‑CCg                                                                                                            <  2:484153/239‑1 (MQ=255)
                                                                                                                                       tcaccacccttctCCCGCGCCCTTGGTACACCCATGCCCTGCCCGA‑CAACCAGCCCAAACGGGGGCTCCCGCGCTTGGTTGTCGGTACAGGTAAGCCCCAGACCCCCAACCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATA‑CCGTCGGACATccc                                                                                                 <  2:922420/231‑1 (MQ=255)
                                                                                                                                             aTCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACCCCCCTTGGTGTCGGGACAGGTAAGCACCAAAACGCCCAACCCCCCCCCGCCCATAAAGGCGGCAGGGGCGGCACCCACCGCCGCACCCACACCCGCCATTTCAGCCAGCCCCCGGGGGATCAGCGGCCGACGCCCCATCGCACCCACACCCACACCCGTCGGCCCTccccccc                                                                                             >  2:1790489/1‑235 (MQ=255)
                                                                                                                                             aTCCTCTCCGTCGCGCTTTGTACCTCCATGCCCTGACCGGGCATCATCCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATA‑CCGTCGGACATCCCCGCAcc                                                                                           <  1:1488841/239‑1 (MQ=255)
                                                                                                                                              tcttctCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCAt                                                                                                                                                                                                                          <  1:2157347/112‑1 (MQ=255)
                                                                                                                                                ttctCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCAGCCCAAACCGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATc                                                                                                                                                 <  1:692572/183‑1 (MQ=255)
                                                                                                                                                        cgcgcTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTTCGCGACTGGGTGTCGGTACAGGGAAGAACCACAACGCCAAGCCCCCCCCCGCCCATaaaa                                                                                                                                                                                                                >  2:2157347/1‑111 (MQ=255)
                                                                                                                                                          cgcTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTAAACGGCCGGGTGTCGGGGCGAGGAAGGAACCGAACGCCAAGACCCGCCATGGCCATAAAGGGGGCAGGGGCGGGACCCAGCGCCGCGCCAAGAAGCGACACTTCAAACAAACCACCGGGGGTCCGCGCCGGAc                                                                                                                                       >  2:692572/1‑183 (MQ=255)
                                                                                                                                                               tGTACATCCATGCCCTGCCCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAgcg                                                                                                                                             <  1:181402/172‑1 (MQ=255)
                                                                                                                                                                 tACCTCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATA‑CCGTCGGACATCCCCg                                                                                               <  1:1382910/215‑1 (MQ=255)
                                                                                                                                                                      ccATGCCCTGACCGAGCATCATGCCAAACCGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGt                                                                                                                                                                                                      <  1:2081696/108‑1 (MQ=255)
                                                                                                                                                                      ccATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGc                                                                                                                                                                                                           <  1:1451623/103‑1 (MQ=255)
                                                                                                                                                                           cccTGACCGACCATCATGCCAAACCGGCGG‑TTCCGCGACTGGTTGTCGGTACGGGAAAGCCCCAAATCCCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGt                                                                                                                                                                                                      <  2:1307901/103‑1 (MQ=255)
                                                                                                                                                                                aCCGAGCATCATGCCAAAACGGCGG‑TTACGCGCCTGGTTGCCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTAcg                                                                                                                                      <  1:1081205/162‑1 (MQ=255)
                                                                                                                                                                                aCCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGGTTTTGGGACAGGGAAAGACCAAAACGCCCAACCCCGCCCTGCCCCTAAAAGGGGGGGGGGCGGGAccccg                                                                                                                                                                                            >  2:2081696/1‑106 (MQ=255)
                                                                                                                                                                                     gCATCATGCCAAAACGGCGG‑TTAAGCGACTGGGTGTCGGTAAAGGGAAGCAACAAATCGCCAAGCCCCCCCATGCCCATAAAGGGGGGAGGGTTCGGACCCaa                                                                                                                                                                                            >  1:1307901/1‑102 (MQ=255)
                                                                                                                                                                                          atGCCAAAACGGCGG‑TTACGCGACCGGGTGTCGGGACAGGGAAGCACCAAAACGCCCAGCCCCCCCCTGCCCCTAAAAGGGGGAGGGGCGGGACCCCGCGCCCCCCCAAGACCCCACATTTCCGCCAGCCCCCGGGGGATCAGCGCCGCACgcgccctccca                                                                                                                            >  2:1081205/1‑155 (MQ=255)
                                                                                                                                                                                              cAAAACGGGGG‑TTACGGGTCGGGTGGTCGGAACGGGTAACCACCAGATCCCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATA‑CCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGaa                                           <  1:83056/238‑1 (MQ=255)
                                                                                                                                                                                                                               cAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATA‑CCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGcc          >  1:1317721/1‑239 (MQ=255)
                                                                                                                                                                                                                               cAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATA‑CCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGc           >  1:1572433/1‑238 (MQ=255)
                                                                                                                                                                                                                                       cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTcagc                                                                                                                                                                 <  2:1181430/81‑1 (MQ=255)
                                                                                                                                                                                                                                       cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTcagc                                                                                                                                                                 >  1:1181430/1‑81 (MQ=255)
                                                                                                                                                                                                                                       cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCg                                                                                                                   <  1:1560797/127‑1 (MQ=255)
                                                                                                                                                                                                                                       cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATA‑CCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTCTGCCGCAGTgca  >  2:1265581/1‑239 (MQ=255)
                                                                                                                                                                                                                                       cACCAAATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCg                                                                                                                   >  2:1560797/1‑127 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          
AAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATA‑CCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCA  >  NC_000913/3782682‑3783150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑