Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	417,533	0	T	G	55.3%	16.5 / 45.7	47	V131G (GTG→GGG)	phoB	response regulator in two‑component regulatory system with PhoR
Reads supporting (aligned to +/- strand):  ref base T (12/9);  new base G (24/2);  total (36/11)
Fisher's exact test for biased strand distribution p-value = 6.48e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-04
Rejected as consensus: Frequency below/above cutoff threshold.

CTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACA‑GAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCA  >  NC_000913/417295‑417734                                                                                                                                                                                                                                               |                                                                                                                                                                                                           ctcGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGGGGCGCGAATCAAAGCGGTAATGCGCCGTATTTGGCCAATGGCGGTGGAAGAGGt                                                                                                                                                                                                            >  1:1149170/1‑239 (MQ=255)                   ggcggcTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGATTGAGATGCAGGGAtt                                                                                                                                                                                          >  1:1365504/1‑239 (MQ=255)                   ggcggcTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGAtt                                                                                                                                                                                          >  1:135142/1‑239 (MQ=255)                   ggcggcTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGAAGAGGGGATTGAGATGCAGGGAtt                                                                                                                                                                                          >  1:1966560/1‑239 (MQ=255)                   ggcggcTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGGATTTCGCCAATGGCGGGGGGAGTGGGGGTTGGGGAGCAggggtg                                                                                                                                                                                          >  2:1283319/1‑236 (MQ=255)                   ggcggcTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGCATTTCGCCAATGGCGGTGGGAGAGGGGATTgtgagtgtggggtt                                                                                                                                                                                          >  2:1054417/1‑229 (MQ=255)                         tCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAGGGGGGGAGTGAGGAGGAGGGATTTAGtc                                                                                                                                                                                     >  2:2263388/1‑238 (MQ=255)                         tCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTTAGATGCAGGGATTAAggc                                                                                                                                                                                     >  1:1660043/1‑236 (MQ=255)                                  cAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGAc                                                                                                                                                                           >  1:251886/1‑239 (MQ=255)                                     ttCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGGAGAGGGGCTTGAGATGCACGGGATAAGACTCGCACCGACa                                                                                                                                                                          >  2:879712/1‑237 (MQ=255)                                       catcaAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGTGCTTTGGATGCAGGGATTTAGTTTCGACCCGACAtct                                                                                                                                                                       >  1:1631210/1‑238 (MQ=255)                                       catcaAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGAGTGAGATGCAGGGAGTAAGTCTCGACCCCGCAtct                                                                                                                                                                       >  2:600475/1‑238 (MQ=255)                                          caAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGACTCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGGTTAATGCTCGACCCGACATCTCAcc                                                                                                                                                                   >  1:507515/1‑239 (MQ=255)                                               acCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGGTTCGACCCGCCATCTCACCGGGTg                                                                                                                                                              >  1:1056481/1‑239 (MQ=255)                                                  tCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGGTGCAGGGATTAAGTCCCGACCCGCACTCCCACCGAGTGagt                                                                                                                                                           >  1:2285258/1‑237 (MQ=255)                                                    aaGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGAAGAGGGGTTTGAAATGCAGGGATTAAGTCTCGACCCGACATCTACCCGAGTGATggc                                                                                                                                                         >  1:2107248/1‑239 (MQ=255)                                                         cgAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGGTTGAGATGCAGGGGTTAAGTCTCGACCCGACATCTCCCCGGGTGATggggggcg                                                                                                                                                    >  2:1085413/1‑239 (MQ=255)                                                           aGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGGGATggcgggggga                                                                                                                                                  >  1:981792/1‑235 (MQ=255)                                                           aGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGAAGAGGGGATTGAGATGCAGGGGTTAAGTCTCGACCCGACATCTCACCGATGGATggggggggga                                                                                                                                                  >  1:1773635/1‑235 (MQ=255)                                                           aGTCGATGACCCGGGATATTCCAGGGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAGGAGGGAGATGAGATGCCGGGGTTAAGTCTCGAGCCGGCATCTCACCGTGTGAGggcgggggga                                                                                                                                                  >  2:792786/1‑235 (MQ=255)                                                                     ccGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGCCATCTCACCGGGTGATGGCGGGGGGGGAGACGCTgg                                                                                                                                        >  1:796961/1‑239 (MQ=255)                                                                      cGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAg                                                                                                                                                                                       >  1:925402/1‑191 (MQ=255)                                                                                     gtgATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGGCTCGACCCGACATCTCACCGAGGGagg                                                                                                                                                           >  1:1843093/1‑202 (MQ=255)                                                                                     gtgATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGGGGGAGAGGGGATTGAGATGCCGGGGTTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGGGGAGAGACGCCGGGGACGGGGGCgcaaca                                                                                                                        >  2:741005/1‑234 (MQ=255)                                                                                        aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGGCGCCGGGGGTGGGGCCGGCC‑GAAtt                                                                                                                    >  1:836411/1‑239 (MQ=255)                                                                                        aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAACCGCTGGAGATGGGGCCGACA‑GAAtt                                                                                                                    >  1:747076/1‑239 (MQ=255)                                                                                        aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACA‑GAAtt                                                                                                                    >  1:478326/1‑239 (MQ=255)                                                                                        aTGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGAGGGCGGGGGGAGAGGCGCAGGGGGAGGGGCCGAccagatt                                                                                                                     >  1:614003/1‑233 (MQ=255)                                                                                          gTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGGGGAGAGACCCTGGGGGTGGGGCCGACA‑GAATTTa                                                                                                                  >  1:1474313/1‑239 (MQ=255)                                                                                                   cAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGAGGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGGGATGGCGGGGGAGGGGCCGCCGgagc                                                                                                                                     >  1:1067982/1‑211 (MQ=255)                                                                                                             gaagaagaTCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGGGGTGGTTGAGATGCCGGGGTTAAGTCTCGACCCGACATCTCCCCGGGTGGTGGCGGGGGGAGGGGCGGCGGGGGAGGGGGCGACACAAATTTAAACTTC‑GCACtttttttt                                                                                               >  2:2262979/1‑237 (MQ=255)                                                                                                                     tCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGCATTTCGCCAATGGCGGTGGAAGGGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGAAATCTCACCGAGTGATGGCGGGGGAAAAGCCGCCGgaga                                                                                                                                     >  1:247540/1‑194 (MQ=255)                                                                                                                        cgTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGGAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGGGGAGAGACGCTGGGGAGTGGGGCGAGC‑GAAGGTAAACTGCCGCACTTTTTTTTTACGGATCCtt                                                                                    >  2:2090215/1‑238 (MQ=255)                                                                                                                                   tggAAACCGGCGCGGATAACTATATCACCAAGCCGTTTGCGCGGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAAGGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACA‑GAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTa                                                                          <  2:2321915/236‑1 (MQ=255)                                                                                                                                                                 aGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCa                                                                                                                                                                     >  1:648651/1‑118 (MQ=255)                                                                                                                                                                     gtattCGCAGAAGGAGGGGGTGGCGCGAATGAAAGCGGGAATGCGTCGTATTTCGCCAATGGCGGTTGAAGAGGTGATGGAGATGCAGGGATTAAGTCGCGACCGGGCATCTCGCCGAGTGATGGCGGGCGAAGAGCCTCTGGAGAGGGGACCGACA‑GAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGaa                                       <  2:346188/236‑1 (MQ=255)                                                                                                                                                                                            cgcgAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACA‑GAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCg                 <  1:385812/238‑1 (MQ=255)                                                                                                                                                                                             gcgAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGGGAAGAGCCGCTGGAGATGGGGCCGACA‑GAATTTAAACTGCTGGACttttttt                                                                                                >  1:990176/1‑157 (MQ=255)                                                                                                                                                                                                           tAATGCGCCGTATTTCGCCAATGGCGGTGGAAGGGGTGATTGAGATGCAGGGATTAAGTCTCGGCCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACA‑GAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCca  <  1:975635/238‑1 (MQ=255)                                                                                                                                                                                                                   cGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGGAGAGGCGCCGGGGGTGGGGGCGCCA‑GAATTTTAACTGCTGCACTTTTTTTTTAAGCCTCCTGAGCGCGTGGACAGCCGCGCGCCGCTGTTTAACCCCGTCTTGGGGACTAACGTGTa                             >  2:191392/1‑203 (MQ=255)                                                                                                                                                                                                                             cAATGGCGGTGGAAGAGGTGAtt                                                                                                                                                                                                        <  2:1863960/23‑1 (MQ=255)                                                                                                                                                                                                                              aaTGGCGGTGGAAGAGGTGATTg                                                                                                                                                                                                       <  2:544593/23‑1 (MQ=255)                                                                                                                                                                                                                                tGGCGGTGGAAGAGGTGATTgag                                                                                                                                                                                                     <  2:1474236/23‑1 (MQ=255)                                                                                                                                                                                                                                tGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGGGATGGGGCCGACA‑GAATTTAAACTGCTGCACTTTTTTATGAc                                                                                            >  1:820667/1‑127 (MQ=255)                                                                                                                                                                                                                                   cGGTGGAAGATGTGATTGAGATg                                                                                                                                                                                                  <  2:1632356/23‑1 (MQ=255)                                                                                                                                                                                                                                    ggTGGAAGAGGTGATTGAGATGc                                                                                                                                                                                                 <  2:925402/23‑1 (MQ=255)                                                                                                                                                                                                                                     gTGGAAGAGGTGATTGAGATGCa                                                                                                                                                                                                <  2:2241565/23‑1 (MQ=255)                                                                                                                                                                                                                                     gTGGAAGAGGTGATTGAGATGCa                                                                                                                                                                                                <  2:2217025/23‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                           CTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTGATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTTTTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACA‑GAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCGATGTCCA  >  NC_000913/417295‑417734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑