| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,005,991 | 0 | G | A | 33.3% | 60.4 / 12.7 | 42 | intergenic (‑205/‑271) | ygeV/ygeW | putative sigma‑54‑interacting transcriptional activator/putative carbamoyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (5/23); new base A (14/0); total (19/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.20e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCTCCATAGCTTTACCTTCCAGACTTACTTAAAAGTCGATCATTGAAGACGTTGATGGTTCACAGATCATGATGATATTAACTCAGGCGAAATTGGCTTTGATAAAAACATAAGATTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAA > NC_000913/3005779‑3006228 | aGCTCCATAGCTTTACCTTCCAGACTTACTTAAAAGTCGATCATTGACGACGTTGATGGTTCACAGATCATGATGATATTAACTCAGGCGAAATTGGCTTTGATAAAAACATAAGATTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGGTTATCAAAATGAATAGAGATAACCCTGCCCTTTTTCTTATTTTTGTTa > 2:454035/1‑239 (MQ=255) aTAGCTTTACCTTCCAGACTTACTTAAAAGTCGATCATTGAAGACGTTGATGGTTCACAGATCATGATGATATTAACTCAGGCGAAATTGGCTTTGATAAAAACATAAGATTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTagc < 1:268903/239‑1 (MQ=255) ttCCAGACTTACTTAAAAGTCGATCATTGAAGACGTTGATGGTTCACAGATCATGATGATATTAACTCAGGCGAAATTGGCTTTGATAAAAACATAAGATTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTc < 1:154124/217‑1 (MQ=255) acttaAAAGTCGATCATTGAAGACGTTGATGGTTCACAGATCATGATGATATTAACTCAGGCGAAATTGGCTTTGATAAAAACATAAGATTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTTTTTAACTGAAGAGAGATAACCTGCCCCTTTAt > 2:154124/1‑199 (MQ=255) acttaAAAGTCGATCATTGAAGACGTTGATGGTTCACAGATCATGATGATATTAACTCAGGCGAAATTGGCTTTGATAAAAACATAAGATTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTTTTAAACTTAAAAGAGATAATCTGCCCCCTTATCTTATTTCTTTTACTTTCAAGCACATAAATAAAACGATaa > 2:345852/1‑239 (MQ=255) atgatgATATTAACTCAGGCGAAATTGGCTTTGATAAAAACATAAGATTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGGTTTTTAAACTGAATAGAGAAAAGCTGCCCCCTTTTCTTTTTTTTGATAATTATCAGCAAATAAAAAAAGCGATAAAAAAAACCAAACGTTTTCGTATTTTACAAACAAACACAAGCt > 2:942724/1‑239 (MQ=255) tatTAACTCAGGCGAAATTGGCTTTGATAAAAACATAAGATTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATTAAAATGAAAAAAGATAAGCTGCCCCTTTTTTTTTTTTTTGATACTTAAAAGCAAAAAAAAAAAGCGATAAAAAAAACCAAAAGGTTTTTTATTTTTAAAAAAAAAACAAGATGGAATCa > 2:1409386/1‑239 (MQ=255) aTTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCtt < 1:277689/114‑1 (MQ=255) aTTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAAt < 1:877639/234‑1 (MQ=255) ttttCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGGGGTTATTAAACTGAAGAGAGATAAACTGCCCCTTTTTTTTATTTTTGATa > 2:277689/1‑114 (MQ=255) ttttCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGGTTTTTAAAATGTAAAGAGATAATCTGCCCCTTTATTTTTTTTTTGTTACTTTACAGCAAATAAAAAAAGCGATAAAAAAAACCAAAAGTTTTTGTATTTTTTAAAAAAACAAAAACTGGCATCAAATTTTGATCAAAACCACACATTATACGCCACATTAGTAtttttc > 2:877639/1‑233 (MQ=255) ttCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCcgc < 2:117443/239‑1 (MQ=255) aaTTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGAt < 1:1050312/188‑1 (MQ=255) tATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTagc < 1:334344/106‑1 (MQ=255) aaGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGGTTCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGca < 1:1409386/238‑1 (MQ=255) aaGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGca < 1:287916/238‑1 (MQ=255) aaGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGca < 1:743094/238‑1 (MQ=255) gagaTATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTTTCAAACTGAAGAAAGAAAACCTTGCCCTTTTTTTTTTTTTTGATAATTAGCAGCAAAAAAAAAAAGCGATAAAAAAAACAAAACGTTTTTGTATTTTTCAAAAAACCAAAAGCCGGGCTCAATTTGTGATCAAACCccca > 2:1050312/1‑188 (MQ=255) agaTATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACt < 1:124900/239‑1 (MQ=255) atatCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGAt < 1:662339/175‑1 (MQ=255) atatCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAAccc < 2:1369232/181‑1 (MQ=255) atatCACATTTCTATATCAATATGAGAATTACGGCGGGGAGGTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTTTCTTATTTTTTATACTTATCAGCaaaaaaa > 2:334344/1‑106 (MQ=255) cacaTTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGt < 1:345852/239‑1 (MQ=255) ttCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTAttt < 1:880589/132‑1 (MQ=255) ttCTATATCAATATGAGAATTACGGCGGTGAGGTTATTAAACTGAAGAGAGATAACCCGCCCCCTTATCTTATTTTTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAACCCAACGTTTTTGTATTTTTCAAAAAACCAAAAGCTGGCATCAATTTTTGTTCAACCCCCCACATTATCCGTCCAATTAGTCTTTTTCCGCCGCGCGGGTAATTTTGGCACACTTATTTTTAGt > 1:62507/1‑238 (MQ=255) ttCTATATCAATATGAGAATTACGGCGGTGAGGTTATTAAAATGAAGAGAGAAAAACTGCCCCCTTTTCTTATTTTTTATACTTTACAGAAAAAAAAAAAAGCGATAAAAAAAAACAAAAGTTTTCGTATTTTAAAAAAAAACAAAAGATGGAAACAAATTGTGATAAAAACCACACATTAACCGTCAAAATAGTCTTTTGCAGCCGCCCGGATAATTTTGTGAAACCTATTGTTatt > 2:191137/1‑236 (MQ=255) tCTATATCAATATGAGAATTACGGCGGGGAGGTTATCAAACTGAAAAGAGATAACCTGCCCCCTTTTCTTTTTTTTTATATTTAACAGCAAAAAAAAAAAGCGATAAAAAAAACCAAACGTTTTTGTATTTTACAAAAAAACAAAAACTGGCATCAATTTTTGATTAAACCCACACAATATCCCTCAAATTAAACTTTTTCAGCCGCGCGGGTAATTCTTGGACACTTATTg > 2:1288452/1‑232 (MQ=255) tCTATATCAATATGAGAATTACGGCGGCGGGTTTTTCAAAATTAAAAGAGATAACCTTGCCCTTTTTTTTATTTCTTATAATTAACCGCCAATAAATAAAGCCATAAAAAAAACCAAACCTTTTTTTATTTTTCAAACAAACAGAAGCTTGCAATCATTTTTCATCAACCCcaca > 2:662339/1‑175 (MQ=255) tCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCaa < 1:272915/85‑1 (MQ=255) tCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTAGTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGta < 1:942724/239‑1 (MQ=255) aaTATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAAttt < 1:1071133/152‑1 (MQ=255) aaTATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATc < 1:1236513/158‑1 (MQ=255) atatGAGAATTACGGCGGTGAGGTTTTCAAACTTAAAAGAGATAGCCTGCCCCTTTATTTTTTTTCTTATACTTAGCAGCAAATAAATAAAGCGATAAAAAAAACCAAACGTTTTTGTATTTTACAAAAAAACAGAAGCTGGCATCAAATTTTGATCAACCCCACACATTATCCGTCAAATTAATCTTTTTCAGCCGCGCGGATAAATTTTGCACACCTATTTTTAGTTCCCGGgata > 1:858738/1‑238 (MQ=255) atatGAGAATTACGGCGGTGAGGTTTTCAAAATGAAGAGAGAAAACCTGCCCCCTTTTTTTTTTTTTTATAATTTACAGCAAAAAAAAAAAGCGATAAAAAAAACCAAACGGTTTTGTATTTTacaaaaaaa > 2:880589/1‑131 (MQ=255) aaTTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAAccc < 1:703111/155‑1 (MQ=255) gTGAGTTTTTCCAACTGAAGAGAGATAACCTGCCCCCTTTTCTTTTTTCTGATACTTAGCAGCAAAAAAATAACGCGATAAAAAAAGCCAAACGTTTTTGTATTTTACAAACAACCAGAAGATGGCATCCATTTTTGTTCATACCCCCCCATTATCCCTCAAATTAATCTTTTTCCGCCCCGCGGGTAAATCTGGCCCCCTTATTGTTTGTCCCCGGTATAACCGTGGAAAAACCAAt > 2:534271/1‑238 (MQ=255) gAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCAc < 2:858738/239‑1 (MQ=255) aTCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGc > 1:1160014/1‑239 (MQ=255) gAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTcaca < 2:1160014/239‑1 (MQ=255) agaTAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGaaa > 1:695811/1‑239 (MQ=255) aTAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCgg > 1:758257/1‑161 (MQ=255) aTAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCgg < 2:758257/161‑1 (MQ=255) gCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTgg > 1:772867/1‑168 (MQ=255) gCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTgg < 2:772867/168‑1 (MQ=255) gCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATaaa > 2:1366451/1‑238 (MQ=255) | AGCTCCATAGCTTTACCTTCCAGACTTACTTAAAAGTCGATCATTGAAGACGTTGATGGTTCACAGATCATGATGATATTAACTCAGGCGAAATTGGCTTTGATAAAAACATAAGATTTTTATCATTTTCTAATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAA > NC_000913/3005779‑3006228 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |