Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091375,0230AC32.1% 84.0 / 10.1 54V153G (GTG→GGG) thiBthiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (19/17);  new base C (0/17);  total (19/35)
Fisher's exact test for biased strand distribution p-value = 1.13e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TAGTTATCTTTCTTCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACAT  >  NC_000913/74789‑75251
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                    
tAGTTCTCTTTCTTCGCTTCGAGAAGGTGATAAGCAGGAGAGGTGGGGTAACTAAGGACCAGTCCGCTTCCACCTTTAAAAAACAGGCCGTAGGCTTCGCCCCATCCTTTGGTGACCGTGCCCGTTTTCTTCGCCCGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGATTTTTTGCATCCATAGCAACAGCCCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCg                                                                                                                                                                                                                                  <  2:89695/239‑1 (MQ=255)
        tttcttCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGtttt                                                                                                                                                                                                                          >  1:1184168/1‑239 (MQ=255)
          tcttctcttcGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGa                                                                                                                                                                                                                        >  1:856608/1‑238 (MQ=255)
          tctcctcttcGAAAATGTGATAAGCGGGAGGGGTGGTGTACCTCAGTCCCAGATCTCTTCCCCCTTTAAAAAACAGCCGTAGGCTTCGCCCCAGCTTTTGGTGACCGTGACCGTTTTCTTCGCCATTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGCCTTTTTGCATCCATAGCAACAGCCCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGAt                                                                                                                                                                                                                       <  2:856608/239‑1 (MQ=255)
                        atgtaagaggCCGGAGAGGTGTGGTACCTCGGTCCCGGTTCGCTTACACTTTTGAAAAGCAGGCCGGGGGCTTCGCTCCGGCTTTGGGTGACCGTGACAGTTTCCTTCGCCAGTTTCTGCCAGGCTTGGGGGGGGTCATCGCCATAGACTTTTTGCATCCAAAGCAACAGACCCAGCCCCGGTGTCCTGGTGCGCGGATCCTAAGAAATCACCCGCCAGTTTTGATCGCTCTCAACCAg                                                                                                                                                                                                          <  2:1035081/230‑1 (MQ=255)
                                 gCCGGAGAGGGGGTGTACCCAGGTCCCAGACCGTTTTCACCTTTAAAAAACAGGCCTTAGGCTTCGCTCCAGCTTTGGGGAACCGTGCCCGTTTTCTTCGCCAGTTTCGGCCAGGCTTGTGGGGCGTCATCGCCATAGCCTTTTGGCATCCATAGAAACAGACCCGGCCCCGGTGTCCTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGctc                                                                                                                                                                                                                  <  2:167689/222‑1 (MQ=255)
                                          gtggtgTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGCTCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTtgtg                                                                                                                                                                                         >  1:635630/1‑238 (MQ=255)
                                           tggtgTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGtt                                                                                                                                                                                                        >  1:167689/1‑222 (MQ=255)
                                              tgtAGCCCAGCACCAGATCGCTTTCCCTTTTTAAAAACAGGCGGTAGGCTACGCGCCAGCCTTTGGTGCCCGAGCCCCTTTTCTTCGCAAGTTTCTGCCAGGTTTGTGGGGCGTCTTCGCTATAGACTTTTTGCATCCACAACAACAGACCCAGCCCCGGTGTCCTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCaa                                                                                                                                                                                                              <  2:225757/213‑1 (MQ=255)
                                                     cAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGgtttttc                                                                                                                                                                             >  1:1170095/1‑237 (MQ=255)
                                                        tACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGgttttttgtt                                                                                                                                                                          >  1:38696/1‑234 (MQ=255)
                                                        tACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTctcgt                                                                                                                                                                          >  1:949918/1‑235 (MQ=255)
                                                        tACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGATCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGgttttt                                                                                                                                                                              >  2:1025663/1‑234 (MQ=255)
                                                        tACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGGGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCttt                                                                                                                                                                                                    >  1:225757/1‑213 (MQ=255)
                                                         aCCAGGTCGCTTTCACCTTTTAAAAACAGGCCGTGGGCTTCCCTCCAGCCTTTGGTGCCCGTGACCTTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGt                                                                                                                                                                           <  2:949918/237‑1 (MQ=255)
                                                             gATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTGGCCAGTTTCTGCCAGGCTTGTGGGGGGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCTCCCGc                                                                                                                                                                                                                                 <  1:1140315/179‑1 (MQ=255)
                                                             gATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGc                                                                                                                                                                                                                                 >  2:1140315/1‑179 (MQ=255)
                                                             gATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGtt                                                                                                                                                                                                                            >  1:611818/1‑184 (MQ=255)
                                                                  cTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCg                                                                                                                                                                                                                                  <  1:158634/173‑1 (MQ=255)
                                                                  cTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCg                                                                                                                                                                                                                                  >  2:158634/1‑173 (MQ=255)
                                                                  cTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGctc                                                                                                                                                                                                                  <  1:159496/189‑1 (MQ=255)
                                                                  cTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGctc                                                                                                                                                                                                                  >  2:159496/1‑189 (MQ=255)
                                                                                aaccAGCCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGCCTTTTTGCATCCATAGCACCAGCCCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGa                                                                                                                                                  <  1:442499/236‑1 (MQ=255)
                                                                                      gCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGc                                                                                                                                                                                                                                 >  2:1206945/1‑154 (MQ=255)
                                                                                      gCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCACCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGc                                                                                                                                                                                                                                 <  1:1206945/154‑1 (MQ=255)
                                                                                             gCTTCCCTCCAGCCTTTGGTGCCCGGGACTGTTTTTTTCGCCAGTTTCGGCCGGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGAt                                                                                                                                                                                                                       <  2:1081273/157‑1 (MQ=255)
                                                                                               tccccTCCGGCTTTGGGAGCCCGTGCCCTTTTTTTTGGCCGTTTTCGGCCGGGCTTAGGGGGCGCAATCGCCATAGTCTTTTGACACCCATAGCACCAGACCCGGCCCCGGTGTCCTGGTGCGGGTATCTTGAAAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCa                                                                                                                                    <  2:295906/234‑1 (MQ=255)
                                                                                                       aGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAAc                                                                                                                                                                                                             >  1:1081273/1‑157 (MQ=255)
                                                                                                          ttgtGGTAACCTTGCCCGTTTCCTTCGCCAGTTTCTGCCAGGCTTGGGGGGCGCCATCGCCATAGTCTTTTTGCATCCATAGCAACAGCCCCAGCCCCGGTGTACTGGTGCGCGTATCCTAATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTAc                                                                                                                            <  2:814334/232‑1 (MQ=255)
                                                                                                                                                     ttGTGGGGCGTCATCGCCATAGACTTTTTGCACCCATAGCACCAGCCCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCg                                                                                                                                                                                                                     <  2:28693/103‑1 (MQ=255)
                                                                                                                                                               tCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGCTCCTGAAAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTc                                                                                                                                                                                                   <  2:852273/111‑1 (MQ=255)
                                                                                                                                                               tCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCa                                                                                                                                                                                                           >  1:28693/1‑103 (MQ=255)
                                                                                                                                                               tCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTc                                                                                                                                                                                                   >  1:852273/1‑111 (MQ=255)
                                                                                                                                                                           aCTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAg                                                                                                                                                      <  1:1134368/144‑1 (MQ=255)
                                                                                                                                                                                        aaaGCAACGAACCGACCCCCGGTGTACTGGGGCCGGACTCCTGTAAATTCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCg                                                                                <  1:369657/199‑1 (MQ=255)
                                                                                                                                                                                        aTAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCa                                                                                                                                    <  1:1084117/149‑1 (MQ=255)
                                                                                                                                                                                            cAACAGACCCAGCCCGGGTGTGCTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCa                                                                                                                                          <  1:1144064/139‑1 (MQ=255)
                                                                                                                                                                                             acaaaccccAGCCCCGTTGTCGTGGTGCGCGGTTCCTAATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTCGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTgg                                         <  1:1002545/229‑1 (MQ=255)
                                                                                                                                                                                                  ccccAGCCCCGGTGTCCTGGTGCGCGGGTCCTTATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCaaa                                                                                                                                  <  1:673224/140‑1 (MQ=255)
                                                                                                                                                                                                     cggCCCGGGTTTCCTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACa                             <  1:1018453/237‑1 (MQ=255)
                                                                                                                                                                                                      aGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGAt                                                                                                                                                                                                                       >  2:160279/1‑52 (MQ=255)
                                                                                                                                                                                                      aGCCCCGGTGTACTGGTGCGCGGATCCGGATAAATCACCCGCCAGTTTTGAt                                                                                                                                                                                                                       <  1:160279/52‑1 (MQ=255)
                                                                                                                                                                                                        ccccGGTGTCCTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGt                                                                                                                                                                           <  1:615582/94‑1 (MQ=255)
                                                                                                                                                                                                              tttACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTAc                                            <  1:29111/213‑1 (MQ=255)
                                                                                                                                                                                                              tgtCCTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGAGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATc                                                                                  <  1:1094304/177‑1 (MQ=255)
                                                                                                                                                                                                                tACTGGTGCGCGGTTCCTAATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCa                                                                                                                                    <  2:712725/125‑1 (MQ=255)
                                                                                                                                                                                                                tACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCa                                                                                                                                          <  1:1114031/119‑1 (MQ=255)
                                                                                                                                                                                                                tACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTggcg                                       <  1:1410927/218‑1 (MQ=255)
                                                                                                                                                                                                                      tGCGCGTCTCCTGAAAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGccc             <  2:38696/238‑1 (MQ=255)
                                                                                                                                                                                                                         gcgGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGc         <  1:610266/239‑1 (MQ=255)
                                                                                                                                                                                                                          cgGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTTTTTTTTTTTTTTTTTAACCAAAGCGGAGGAGCCCTAATCAAAca                                                                                                                                >  2:1114031/1‑118 (MQ=255)
                                                                                                                                                                                                                           ggATCTTTATAACTCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGca        <  1:320607/238‑1 (MQ=255)
                                                                                                                                                                                                                            gATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGcac       <  1:1025663/238‑1 (MQ=255)
                                                                                                                                                                                                                                 ttGAAAAACCCCCCGCCAGTTTGGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAGGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCGGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACAt  <  1:814298/238‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                    
TAGTTATCTTTCTTCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACAT  >  NC_000913/74789‑75251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: