Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,665,7020CA30.6% 194.0 / 24.3 85A37S (GCG→TCG) gadXacid resistance regulon transcriptional activator; autoactivator
Reads supporting (aligned to +/- strand):  ref base C (51/8);  new base A (0/26);  total (51/34)
Fisher's exact test for biased strand distribution p-value = 3.60e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGT  >  NC_000913/3665468‑3665933
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                       
ttttACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCaaaa                                                                                                                                                                                                                                     >  2:1010122/1‑239 (MQ=255)
ttttACGACGCATTTCTGTATAGCGAAGATTCTTATTCTGCGATAGTTGGGCAACTTCCTGAAAAACAGGACTTTCCCTACTACAGTGATGAACGAGTAATGCAGAAATTTTTTTATGTGCATGTAGATTTAATGCCTCCTCCTTGAGCACCGGGAGACATAATGAAATCTTGGCTCTTGACCCTTATCTAAAATTTTCAACACACTTATCTACTCGAGTTTGGCCTTCTTCCGCacac                                                                                                                                                                                                                                     >  2:1284140/1‑236 (MQ=255)
   tACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAAcc                                                                                                                                                                                                                                  <  2:316979/239‑1 (MQ=255)
       tAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAgg                                                                                                                                                                                                                               >  2:193280/1‑238 (MQ=255)
        aGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTc                                                                                                                                                                                                                             >  2:1204319/1‑239 (MQ=255)
          cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCa                                                                                                                                                                                                                            >  1:451060/1‑238 (MQ=255)
          cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCa                                                                                                                                                                                                                            >  2:1113157/1‑238 (MQ=255)
          cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCa                                                                                                                                                                                                                            >  2:165141/1‑238 (MQ=255)
           ttttCTGCATAGCGAATATTCTTGTTCTGCGATGTTTACGAAATTTCCTGAAAAACAGGAATATCTCTACTACATTGATGAACGAGAAATGAAGAATCTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAAAAATAATGAAGGCGTGTCCCTTAACACAAATAAAAAATTTCCACCCCACTTTTCTACTCGAATTTGGCTTGCATCCGCAAAAACCGGGTCAcc                                                                                                                                                                                                                          <  2:183830/238‑1 (MQ=255)
           aTTTCTGCATAGCGAAGATTCTTTTTCTGCGATAGTGGCGCAACTTCCTAAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATAAAAAAACTTTTTTATGTGCATGAAGATTTAATCCCTCCTCCTTGAGCATCGGGAAAAAAAATGAAAGCGTGCCCCTTGACACAAATAAAAAATTTTCAACCCACTTATCTCCTAAATTTTGGTTTGCACCCCCAAAAACCAGGTCAcc                                                                                                                                                                                                                          <  2:1194573/239‑1 (MQ=255)
               cTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCa                                                                                                                                                                                                                      >  1:1008398/1‑239 (MQ=255)
                  cATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTa                                                                                                                                                                                                                   >  2:996687/1‑239 (MQ=255)
                  cATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTa                                                                                                                                                                                                                   >  1:183830/1‑239 (MQ=255)
                   aTAGAGAAAATTTTTTTGCTGCATTAGTTGCACACCTCCAAAAAAACAAGAAATTTTCTTACTAAAGTGAGGAAAGAGAAAAAAAAAACTTTTTTTATGGCAATGAAGTTTTAATCCCCCCTCCTTAAGCATGGGAAAAAAAAATGAAAGCGTGCCCCTTGACAAAAATAAAAAATTTCCAACACCCTTTTTTACCCGATTTTGGCTTGCACCCGAAAAAACCAGGTCACCGCCATTaa                                                                                                                                                                                                                  <  2:1008398/239‑1 (MQ=255)
                             tttttcttCGGCGATTGATGCACAACTTCAGAAAAAACAGGAATATCTCTACTAAAGTGATGAACGGGTAATAAAGAAACTTTTTAAGGTGAATAAAGATTTGATGCCTCCTCCTTGAGCACCGGGAAAAATAATGAAGGCGTTTCCCTTGACACAAAAACAAAATTTTCAACACTTTTATCTTCTCAATTTGGGCTTCCACCCACAAAAACGGGGCCTCCGCCATTAAAATAg                                                                                                                                                                                                             <  2:362752/228‑1 (MQ=255)
                               ttttctgtGCGAGTGTTGCACAATTTACTAAAAAACAGAAATATCTCTACTAGAGTGATAAACGTGTAATAAAGAAATTTTTTTATGTGCATAAGGATTAAAGCCCTCCTCCTAAAGCTTCGGGAGAAAAAATAAACGCTTCTCCCTTCACAGAAATAAAAATTTTTCAACCCCTTTTCCTCCAAAATTTTGTTTGGCCCCCAAAAAACCCGGGTCCCCGCCATTAAAATAGCGATAtt                                                                                                                                                                                                      <  2:411033/232‑1 (MQ=255)
                               tttATTCTGAGATAGTTGCGCAACTTCCGGAAAAACAGGAATATCTCTACTAAAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGACCAGCGGGAAAAAAAATGAAAGCGTGTCCCTTGACACAAATAAAAAATTTCCAACACACTTATCTCCTAAAATTTGGCTTGCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATAtt                                                                                                                                                                                                      <  1:366630/238‑1 (MQ=255)
                               cttttttggCGATAGTTGCGCAACTTCTTAAAAAACAGAAATTTCTCTACAAAAGTGAGGAACGAGTAATGAAAAAATTTTTTTATGTGCAGGAAGATTTAAGCCCTCCTCCTTGAGCATCGGGAAAAATAAGGAAAGCGTGTCCTTTGACACGAAAAAAAAATTTTCAACACACTTATCTACTCAAATTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATAtt                                                                                                                                                                                                      <  1:165141/231‑1 (MQ=255)
                               cTTTTTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGAAATATCTCTACTACAGTGATGACCGAGTAATGAAGAAATTTTTTTTTGTGCAAGAAGATTTAATCCCTCCTCCTTGACCATGGGAAGAAAAAATGAAGGCGTGCCCCTGGACACAAATAAAAATTTTTAAACCCTTTTATCTCCTCGATTTTGGCTTCCCCCCGAAAAAACCGGGTCACCGCCATTAAAATAGCGATAtt                                                                                                                                                                                                      <  1:996687/239‑1 (MQ=255)
                               cTTATTCTGAGATTGTTGCACAATTTCCAAAAAAACAGAAATATCTCTACTACAGGGAAGACCGAGTAATGAAAAAACTTTTTTATGGGCATAAAGATTTAATCCCCCCCCCTAGAGCATCGGAAAAAAAAATAAAAGCGTTCCCCTTGACACAAATAAAAAATTTTCAACCCCCTTATCTACTCAATTTTGGCTTCCACCCCCAAAACCCAGGTCCCCGCCATTAAAATAGCGATAtt                                                                                                                                                                                                      <  2:451060/239‑1 (MQ=255)
                                      tGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCAtt                                                                                                                                                                                               >  1:1205342/1‑239 (MQ=255)
                                      tGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACAAGGTCACCGCCATTAAAATAGCGATATTCACCAtt                                                                                                                                                                                               >  1:347808/1‑239 (MQ=255)
                                        cGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTa                                                                                                                                                                                              >  1:231472/1‑238 (MQ=255)
                                          aTAGTTGCCCAACTTCCTAAAAAACAGGAATATCTCTACTACAGTGATAAACAAGTAATGAAGAATCTTTTTTATGTGCATGAAGATTTAATCCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAAAACAAAATTTTCAACACCCTTATCTCCTCAATTTTGGCTTGCACCCGAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAAc                                                                                                                                                                                            <  1:590905/238‑1 (MQ=255)
                                          aTAGTTCCCCATTTTCCAAAAAAACAGAAATATCTCTACTAAAGTGAAAAACGGTTAATAAAAAATTTTTTTTTTGTACATGAAGTTTTAATGCCTCCCCCTAGAGCATCGGAAAAAATAATGAAACCGTCTCCCTTGACACAAATAAAAAATTTTCAACACATTTATCTCCTAGATTTTGCCTTGCATCCGAAAAACCCGGTTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                                                                                                           <  1:566294/239‑1 (MQ=255)
                                               tgcaaaatttCCTAAAAAACAGAAATTTCTCTACTAAAGAGATGAAAGAGTAAAGAAAAAATTTTTTTATGGACATGAAGTTTAAAAGCCTCCCCCTTGGCAAGCGGGGAAAATAGTAAAAGGGTGTCCCTTGACTCAAAAACAAATTTTTCAAAACTCTTATCTCCTCGATTTTGTTTTGCATCCGAAAAAACCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                                                                                                      <  1:390803/231‑1 (MQ=255)
                                               tGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGAGAAACGAGTAATGAAGAATTTTTTTTATGTGCATGAAGATTTAATCCCTCCTCCTTGAGCATGGGGAAAAATAATAAAGGCGTGTCCCTTGCCACGAATAAAAAATTTTCAACCCCTTTATCTCCTCGATTTTGGCTTCCATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                                                                                                      <  1:1204319/239‑1 (MQ=255)
                                                gcgcAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAACCTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                                                                                                                     >  1:875847/1‑239 (MQ=255)
                                                   cAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAACATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTgaga                                                                                                                                                                                  >  2:590905/1‑239 (MQ=255)
                                                      cttacaaaaaaaCAGAAATATCTCTAATAAAGTGATAAACGAATAATGAAAAAATTTTTTTATGGGAATGAAGATTTAACGCCCCCTCCTTGAGAAGGGGAAAAAAAAATAAAAGCGTGTCCCTTAACAAAAAAAAAAAATTTTCAACACACTTATCTACACAATTTTGGCTTGCACCCCAAAAACCCGGGTCACCGCCATTAAAATAGCGATAtt                                                                                                                                                                                                      <  2:1393899/209‑1 (MQ=255)
                                                          aaaaaaaaCAGGAATATCTTAACTACGTTGATGAACGATTAAAAAAGAATTTTTTTTATGTGAATGAAATTTTAATGCCTCCCCCTTAAGAATGGGAAAAAATAATGAAGGCGTGCCCCTTGCCACAAAAAAAAATTTTAAACCCCCCTTTTTTACTAAATTTGGGCTTCCCCCCGAAAAACCCAGCCCACCGCCATTAAAATAGCGATAtt                                                                                                                                                                                                      <  2:875134/209‑1 (MQ=255)
                                                                aaCAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAc                                                                                                                                                                                            >  1:1393899/1‑216 (MQ=255)
                                                                   aGGAATATTTCTACAAAGTTAATAAACGAGTAATGAAAAAATTTTTTTATGTGCAAGAAATTTTAATCCCCCCTCCTTGACCATCGGGAGAAATAATGAACGCGTGTCCCTTGACACGAATAAAAAATTTCCACCCCACTTATCTACTCAATTTTGTTTTACACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCAt                                                                                                                                                                                                <  2:100938/209‑1 (MQ=255)
                                                                    ggAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAc                                                                                                                                                                                            >  1:875134/1‑212 (MQ=255)
                                                                         atCTTTACTAAAGTGATGAACGAGTAAAAAAAAATCTTTTATATGTACAAGAATATTTAAGGCCCCCTCATAGAGCATGGGGAAAAAAAAGAAAAGCGTGTCCCTTGACACAAAAAAAAAATTTCCAACACTCTTATCAACTAAATTTTGGTTTCCACCCAAAAAAACGAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACg                                                                                                                                                            <  2:87952/239‑1 (MQ=255)
                                                                            tctACTAAAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGTTTTAATCCCTCCTCCTTGAGCATGGGGAAAAAAAATGAAAGCGTGTCCTTTGACCCGAATAAAAAATTTTCAACACTTTTATCTACTCAATTTTGGCTTGCACCCGCAAAACCCAGGTCACCGCCATTAAAATa                                                                                                                                                                                                              <  1:48123/186‑1 (MQ=255)
                                                                             ctactaCAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                                                                                                      >  1:100938/1‑209 (MQ=255)
                                                                               tactacATTGATGAGCGAGTAATGAAAAAATTTTTTTATGTGAAGGAAGATTTAATGCCTCCTCCTTGAGCATGGGGAGAAATAATAAAAGCGTGTCATTTGACAAAAAAACAAAATTCCCAACACCCTTATCACCTGAAATTTGGCTTCCACCCGAAAAAACCAGGTCACCGCCATTaaaa                                                                                                                                                                                                                <  1:628948/182‑1 (MQ=255)
                                                                                actacAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCAc                                                                                                                                                                                                   >  1:674767/1‑194 (MQ=255)
                                                                                  taaggTGATAAACAATAAATGAAAAAATTTTTTTATGTTCAAAAATATTTAACCCCCCCCCTGTGAGAATCGGAAAAAAAAATGAAGGCCGTTCCTTTGACAAAAAAAAAAAATTTCAACCACTCTTCTCTACAAAATTTTGGTTTGCCCCCAAAAAACCGGGGACACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGa                                                                                                                                                    <  2:721205/235‑1 (MQ=255)
                                                                                     aGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACa                                                                                                                                                  >  1:87952/1‑238 (MQ=255)
                                                                                       tgatgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCa                                                                                                                                                                                                    >  2:48123/1‑186 (MQ=255)
                                                                                       tgatgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAt                                                                                                                                                >  1:650840/1‑238 (MQ=255)
                                                                                       tgatgaACGAGCAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGCAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAt                                                                                                                                                >  1:1370156/1‑238 (MQ=255)
                                                                                         atgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTaaaa                                                                                                                                                                                                                >  1:343202/1‑172 (MQ=255)
                                                                                         atgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATAtt                                                                                                                                                                                                      >  2:628948/1‑182 (MQ=255)
                                                                                         atgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                                                                                                                 >  1:1100297/1‑203 (MQ=255)
                                                                                         atgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                                                                                                              >  1:851861/1‑238 (MQ=255)
                                                                                         atgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                                                                                                              >  2:295695/1‑238 (MQ=255)
                                                                                                aGTAAGAAAAAAATTTTTTAATGTAAAAAAAGTTTAAACCCCCCCTCCTGGACAATGGGAAAAAAAAATAAAGGCTTGCCCCTTGACACAAAAAAAAAATTTTAACCCCACTTATCTACTAAATTTTGTTTTGCACCCAAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                                                                                                                      <  2:650840/239‑1 (MQ=255)
                                                                                                   aaTGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATGGGGAAAAAAAATGAAAGCGTGTCCCTTGACACGAATAAAAAATTTTAAACACACTTATCAACTCGAATTTGGCTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGt                                                                                                                                     <  1:1113157/237‑1 (MQ=255)
                                                                                                       aagaaACTTTTTTATGTGCATGAAGATTAAATCCCTCCTTCTTGAGCATCGGGAAAAATAATGAAAGCGTGTCCCTTGAAAAGAAAAAAAAATTTTCAACACACTTATCTACTCAAATTTGGCTTGCATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGt                                                                                                                                     <  2:355055/233‑1 (MQ=255)
                                                                                                         gaaACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                                                                                                             >  1:898078/1‑239 (MQ=255)
                                                                                                                         cATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCTTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATAtt                                                                                                              >  1:1066916/1‑238 (MQ=255)
                                                                                                                              attttaactGCCTCCTCCGTGAGAAGCGGAAAAAAAAATAAAAGCTTGTCCCTAGACAAGAAAAAAAAATTTCCACCCATCTTACCTAATCAAATTGGGTTTCCAACAAAAAAACCCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                                                                                                          <  2:898078/229‑1 (MQ=255)
                                                                                                                                    aaTGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCa                                                                                                                                                                >  1:871743/1‑177 (MQ=255)
                                                                                                                                           cctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATAtt                                                                                                 >  2:972201/1‑233 (MQ=255)
                                                                                                                                           cctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATAtt                                                                                                 >  1:1292750/1‑233 (MQ=255)
                                                                                                                                                 tGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTTAACACACTTATTTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATATCGATATTCCTCATTAACCATGGGGAGAATATACTTATGTTTTGCATACGCAATTAGACAATTCCCATGTa                                                                                                                                       >  1:940589/1‑189 (MQ=255)
                                                                                                                                                     cATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAAGTTGGCTTGCATCCGCAAAAACCCGGTCACCGCCATTAAAATAGCGATATTCAACATTAACCATGGTGAGAATATAttt                                                                                                                                                                          >  1:1286313/1‑150 (MQ=255)
                                                                                                                                                        cGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGc                                                                               >  1:115708/1‑238 (MQ=255)
                                                                                                                                                        cGGGAGAAATAATGAAAGCGCGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCCGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCACTTAGACCATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATCTTACTGTTTATTAATGTAGc                                                                               >  1:919523/1‑238 (MQ=255)
                                                                                                                                                                   atGAAGGCGTTTCCCTGGACACAAAAAAAAAATTTTCAACACTCTTTTCTACGAAATTTTGGTTTGCATCCACAAAACCCAGCCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                                                                                                                   <  2:1174101/159‑1 (MQ=255)
                                                                                                                                                                    tgaaggcggcccccTAGACACAAAAAAAAATTTCCAAACACCTTTATCTTCAAAATTTTGGTTTACACCCGAAAAACCCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCtt                                                                  <  2:875847/228‑1 (MQ=255)
                                                                                                                                                                    tGAAGGCGGGTCCCTTGACACGAATACAAATTTTTCAACCCATTTATCTACTGGAATTGGCTTTGCACCCCAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCtt                                                                  <  1:193280/239‑1 (MQ=255)
                                                                                                                                                                    tGAAAGCGTTTCCCTTGACACAAATAAAAAATTTTAACCACATTTATCTACTCAATTTGGGTTTCCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                                                                                                      <  2:1394723/123‑1 (MQ=255)
                                                                                                                                                                     gAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGa                                                                                                                                                    >  1:251060/1‑156 (MQ=255)
                                                                                                                                                                          cGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCa                                                            >  1:1158635/1‑239 (MQ=255)
                                                                                                                                                                            tgtCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGc                                                                                                                                                                                                            >  1:455647/1‑93 (MQ=255)
                                                                                                                                                                             gtCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATg                                                                                                                                         >  1:1174101/1‑159 (MQ=255)
                                                                                                                                                                              tCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCACGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatatat                                                                                                                                                                            >  1:1394723/1‑123 (MQ=255)
                                                                                                                                                                                  ttGACACGAAAAAAAAATTTTCAACCCCCTTATCTACTCAAATTTGGCTTGCACCCGAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                                                                                                                   <  2:1305075/144‑1 (MQ=255)
                                                                                                                                                                                         gAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTa                                                                                                >  1:538622/1‑188 (MQ=255)
                                                                                                                                                                                            tACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAGATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATg                                                                                                                                         >  1:1305075/1‑144 (MQ=255)
                                                                                                                                                                                                   ttttCAACCCTCTTTTCTCCTCAATTTTGGTTTCCACCCCAAAAACCCAGGTCCCCGCCATTAAAATAGCGATATTCACCAtt                                                                                                                                                                                               <  2:685167/83‑1 (MQ=255)
                                                                                                                                                                                                       caacaaACTTATCTTAACGAATTTGGTTTGCATGCGCAAAACCCAGCTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAg                               <  1:1010122/239‑1 (MQ=255)
                                                                                                                                                                                                               acTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                                                                                                                     >  1:685167/1‑83 (MQ=255)
                                                                                                                                                                                                                     ctactaataTTTCGCTTGCACCCAAAAAACCCAGTCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACCATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAgg                   <  2:1066916/231‑1 (MQ=255)
                                                                                                                                                                                                                         tCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGgatga               >  2:212602/1‑239 (MQ=255)
                                                                                                                                                                                                                         tCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGgatga               >  1:890054/1‑239 (MQ=255)
                                                                                                                                                                                                                         tCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGAGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGgatga               >  1:1291625/1‑239 (MQ=255)
                                                                                                                                                                                                                             ttttGGCTTACACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                                                                                                                   <  2:573555/102‑1 (MQ=255)
                                                                                                                                                                                                                                        cATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCAt                                                                                                                                          >  1:1136493/1‑102 (MQ=255)
                                                                                                                                                                                                                                        cATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATg                                                                                                                                         >  1:573555/1‑103 (MQ=255)
                                                                                                                                                                                                                                        cATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGt  >  1:1277101/1‑238 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                       
TTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGT  >  NC_000913/3665468‑3665933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: