Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,689	0	G	T	31.6%	51.3 / 14.2	57	intergenic (+43/+22)	yegJ/yegK	uncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (25/14);  new base T (10/8);  total (35/22)
Fisher's exact test for biased strand distribution p-value = 5.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.49e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTG‑TTGTC‑TT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACC  >  NC_000913/2151484‑2151914                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       aaaGGTAGAATGTGGCAAGGAGGAAAAAATAAAAAAAGAAAATTTTTAAGACGGGTTTTAGCGGCGGGGGAATAAAATTCAGGGAGGTTATCCCTTTGATCTTTTTTTGTCCCCCTACAAAAAAGAAAAGCCCGACGAACAAAAAGAAAATT‑TATTTCG‑TTAACCAGCCTTTATTTTTTTTTTTAAAT‑CGGGC‑GT‑TTAAATTG‑TTGTT‑TT‑AAACCGCCATGAGAAACTCACTCTGCCca                                                                                                                                                                                                     <  2:1006023/239‑1 (MQ=255)                     ggTGGAAAATCAAGAAAGAAAATATTTCAGACTGTATGTATACGCGGGGGGAAAAAATTTACGGCGTTTATACCATTAATCCTTTATTGATCACCTATACAAAAGAAGAAGCAAAAGAGATAAGAGAAAAAT‑TAGTTCA‑CTAAGCAGCCGTTATATTTTTTGTTAAAA‑CGGGC‑GT‑TTGAATTG‑TTGTC‑TT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑Gtt                                                                                                                                                                                <  1:1102678/237‑1 (MQ=255)                                                       gATGTATACGCGGGGGAATAAAATTTACGGGGGTTATACCATTGATCCTTTATTGGTCACCTACCAAAAAGAAGAAGCCGACGAGCAAAGAGAAAAAT‑TATTTCG‑CTAATCAGCCTTTATATTTATTGTTTAAA‑CGGGC‑GG‑TTAAATTG‑TTGTC‑TT‑CAACCGCCATGAGAAactcact                                                                                                                                                                                                            <  1:522193/178‑1 (MQ=255)                                                                                      ctattaaCCAATTATACTTTATTGGTCACCTAAAAAAAAAAAATAGCCGGAGAAATAAGAAAAAAATGTATTTC‑‑CTAAAACGCCCTTTTTTTTTTTGTTTAAACCGGGC‑GG‑TTGATTTG‑‑TGTCATA‑CACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAg                                                                                                                  <  2:1290523/229‑1 (MQ=255)                                                                                       tttATCCCTTTAATCTTTTATTGGTCCCCTATCAAAAAAGAGAAGCCGATGAGCGAAGAAAAAAAT‑TATTGCG‑CAAATCACCCGTCATATTTTTTGTGAAAA‑CGGGC‑GT‑TTGAATTG‑TTTTC‑TA‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTATCATCATCCGTa                                                                                                                                                                 <  1:1025678/186‑1 (MQ=255)                                                                                        ttATCCCATTATTCTTTTTTTGTCCCCCTACCAAAAAAAAAAACCCAGCGAGCAAAGAGAAAAAT‑TAGTTCG‑TAAATCAGCCTTTATATTTTTTGTTAAAC‑CGGGC‑GG‑TTAATTTT‑TTTTC‑TT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGc                                                                                                                               <  1:54007/219‑1 (MQ=255)                                                                                        taataCCTTTGATCCTTTATTGGCCCCCTACCCAAAAGAAGAAGCCGAGGAGCTAAGAGCAAATT‑TATTTCT‑CTAACCGGCCGTTATAAGTTTTGTTTAAA‑CTGGC‑GG‑TTGAATTG‑TTTTC‑TC‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCa                                                                                                                                                                                             <  1:1400314/158‑1 (MQ=255)                                                                                                 tGATCCTTTATTGGTCACCTATCCAAAAGAAGCAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTG‑ATGTC‑TT‑CTAACGCCATGAGAAACACCCTCCGCCCACAACGCCCGTG‑CCAAC‑GTTTTATCATCATCCGTATGCTCGTTTA‑CCGCCCGGCTGGGCaaaa                                                                                                                                    >  1:297199/1‑205 (MQ=255)                                                                                                 tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTTAATTT‑TTTTC‑TT‑TAAACGCCATTAGAAACTTACTCTGCCCCCCGCGCCCGTG‑CCAAC‑GTTTTATTATCATCCTTACCCTCGtttt                                                                                                                                                       >  2:1025678/1‑186 (MQ=255)                                                                                                 tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGTATTG‑TTGTC‑TT‑CAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTATCATCATCCGTACGCCCGTTTA‑ccg                                                                                                                                                   >  1:1149950/1‑190 (MQ=255)                                                                                                  gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTT‑TTTTC‑TT‑CAACCGCCATGAGAAAATCACTCTTCCCCCAGCGCCAGTG‑CCCAcag                                                                                                                                                                                  >  2:1400314/1‑158 (MQ=255)                                                                                                  gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTG‑TTTTC‑TT‑CAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GCTTTTTCATCATACGTACGCTCGTTTA‑CCGCCGGGCTGGGCAAAAACTGCTTTTACCAtt                                                                                                                     >  2:54007/1‑219 (MQ=255)                                                                                                  gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTG‑TTGTC‑TT‑CAACCCCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTG‑CCAAC‑GTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGGAATAAATCGAgttgt                                                                                                 >  1:1126303/1‑239 (MQ=255)                                                                                                  gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTG‑TTGTC‑TT‑CAAACGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTATCATCATTCGTACGCTCGCTTA‑CCCCCGGGCTGGACCAAAACCGCTTTTAAAATTCAGGCAATAAAACGAgttgt                                                                                                 >  1:44551/1‑239 (MQ=255)                                                                                                   aTCTTTTTTTGGTCCCCTACCAAAAAAAAGAAGCCGAGGAGAAAAGAGAAAAAT‑TATTTCG‑CTAACCAGCCTTTATATTTTTTGTTAAAC‑CGGGC‑GG‑TTGAATTG‑TTTTC‑TT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGc                                                                                                                                             <  1:222114/194‑1 (MQ=255)                                                                                                    tcttttATTGCTCACCTACCAAAAAAAAAAACCCCACGAGAAAAGAGAAAAAT‑TATTTCT‑CTAACCACCCTTTATATTTATTTTTAAAC‑CGGGG‑GG‑TTAATTTT‑TTGTC‑TC‑AAACCGCCATGAGAAACTCACTCTGCCca                                                                                                                                                                                                     <  2:1119016/137‑1 (MQ=255)                                                                                                       tttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTG‑TTGTC‑TT‑CAACCGCCATGAGAAAATCACTCTGCCcac                                                                                                                                                                                                    >  1:570926/1‑138 (MQ=255)                                                                                                       tttATGGGCCACCTACCAAAAAAAAAAAGCGGAGAAGTAAAGAGAAAAAT‑TAGTTGG‑CAAACCAGCGGTTATTTTTATTGTTAAAC‑GGGGG‑GG‑TGGATTTG‑TTTTC‑TT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑g                                                                                                                                                                                  <  1:1286018/154‑1 (MQ=255)                                                                                                        ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGCTGTG‑TTGTC‑TT‑CCACCGCCATTAGTAAATAACTCTGCCCACAGCGCCAGTG‑CCCAC‑GCTTTTTCATCATCCGTACGCTCGTTCA‑CCGCCCGGCTGGACAAAAAATGCTTTTAAAATTCAGGGAATAAATAGAAATGTTCCtca                                                                                           >  2:751730/1‑237 (MQ=255)                                                                                                        ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTT‑TTTTT‑TT‑CAACCGCCATGAGAAAATCCCTCTTCCCACAGCGCCAGGT‑CCAAC‑Gtttttt                                                                                                                                                                            >  2:736185/1‑157 (MQ=255)                                                                                                        ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTC‑TTTTT‑TT‑CTACCGCCATTGGAAAACTACTCCTGCCACAGGGCCCCTGCCCAAC‑GTTTT‑TCATCACCCCGAATCTCGTTTATCACCCGGCCCGGGAAAAAATGGCTTTAA‑AATTCCGGGAATAAATCGCattgtttcctc                                                                                           >  2:337361/1‑234 (MQ=255)                                                                                                        ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAAATG‑TTTTT‑TT‑CAACCGCCATGAGAAAATCACTCTGCCCCCAGCCGCAGGG‑CCAAC‑CTTTTTTCATCATCCCTAACCCCGTTTA‑CCGCCCGGCGGGGCAAAAACTGCTTTAACAATTTCGGGAAAAAATCCAGTTTTTTCTGc                                                                                           >  2:28313/1‑239 (MQ=255)                                                                                                             ggTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTT‑TTGTC‑TT‑CAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTTTCATCATCCGTACGCTCGTTTA‑CCCCCCGGCTCGGCaaaaa                                                                                                                                   >  2:222114/1‑194 (MQ=255)                                                                                                              gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTTAATTG‑TTGTT‑TT‑CAACCGCCCTGTGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCCGGCTGGGCAAAAACTGCTTTAACAATTCAGGt                                                                                                                >  1:322972/1‑212 (MQ=255)                                                                                                              gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑GTGAATTG‑TTGTC‑TT‑CAACCGCCATGAGAAACTCACTCTTCCCACAGCGCCAGt                                                                                                                                                                                           >  1:1119016/1‑140 (MQ=255)                                                                                                                 cccTACCCAAAAGAAGAGGCCGTCGAGCAAAGGGAAAAAT‑TA‑TTCGACTAATCGGCTTTTATATTTATTTTTAAAG‑CTGGA‑GT‑TTGAATTG‑TTGTC‑TC‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑cc                                                                                                                                                                                       <  1:861187/139‑1 (MQ=255)                                                                                                                 aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTG‑TTTTC‑TT‑CAACCCCCATGAGAAACCCACTCTTCCCACAGCGCCAGTG‑CCCAC‑GTTTTatcatc                                                                                                                                                                        >  2:1286018/1‑154 (MQ=255)                                                                                                                 aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTG‑TTGTC‑TT‑CAACCGCCCTGAGAAACTCACTCTGCCCCCAGCGCCAGTG‑CCAAC‑GTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGGAATAAATCGAGATGTTCCTGCGTTGCTgcc                                                                                  >  1:125081/1‑239 (MQ=255)                                                                                                                   cTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTTATCAGCCGTTATATTTATTGTTTAAA‑CAGGC‑GG‑TTGGACTG‑TTGTG‑TT‑CAAACCCCACGAGAAAAATACTCTTcccc                                                                                                                                                                                                     >  1:1169414/1‑124 (MQ=255)                                                                                                                      tCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTT‑TTTTC‑tt                                                                                                                                                                                                                                   >  2:748083/1‑93 (MQ=255)                                                                                                                      tCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTG‑TTGTC‑tt                                                                                                                                                                                                                                   <  1:748083/93‑1 (MQ=255)                                                                                                                       ccAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTG‑TTGTC‑TT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGGG‑CCAAC‑GTTTTATCATCATCCGTACGCCCGGTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTgccg                                                                                 >  1:564645/1‑234 (MQ=255)                                                                                                                           aagaagaagCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTCAAATG‑TTTTT‑TT‑TAAACGGCATGAGAAACTCACTATGTCCACAGCGGCAGGG‑GCAACAGttttt                                                                                                                                                                             >  2:861187/1‑139 (MQ=255)                                                                                                                                 aagCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTTAATTG‑TTGTC‑TT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GGTTTATCATCATCCGTACGCCCCTTTA‑CCCCCGGGCTGGACAAAAACTTC‑TTAACAATTTAGGTAATAAAACGAATTGTTCCCGCGTTGCTGCCCCCAGTCCACTGaaaaa                                                                 >  1:816087/1‑239 (MQ=255)                                                                                                                                   gCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTT‑TTGTC‑TT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTATCATCATCCGTACGCTCGTTTA‑CCCCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGGAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAAc                                                                >  1:1088607/1‑239 (MQ=255)                                                                                                                                                       aT‑TAGTTCG‑TAAATCA‑CCTTTTTTTTTTTTTTTTAAA‑CCTGG‑GG‑TTTAATTT‑TTTTT‑TTACACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATg                                            <  1:142831/239‑1 (MQ=255)                                                                                                                                                            gTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTG‑TTGTT‑TT‑CAACCGCCATGAGAAAATCACTCCGCCCCCAGCGCCCGTG‑CCAAC‑GTTTTTTCATCATCCCTACGCCCGTTTA‑CCGCCGGGCCGGACaaaaa                                                                                                                                   >  1:451416/1‑148 (MQ=255)                                                                                                                                                                  cTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTTAATTT‑TTTTC‑TT‑CAACCGCCATGAAAAACTCACTCTGCCCACAACGCCAGTG‑CCCAC‑GTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCCGGCTGGAAAAAAACTGCTTTAAAAAATCCGGGAATAAATCGAGTTGTTCCTGCGTTGCTGCCCCCAGTCCATTGAAAAAAGGGGGAAAGAAAAGCACATGAGGAGaaat                                   >  2:180205/1‑237 (MQ=255)                                                                                                                                                                  cTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTG‑TTGTC‑TT‑CAACCGCCCTGAGAAACTCACCCTGCCCACAGCGCCCGGG‑CCAAC‑GGTTTTTCATCATCCCTACGCCCGTTTA‑CCGCCGGGCTGGGCaaaaa                                                                                                                                   >  1:956811/1‑143 (MQ=255)                                                                                                                                                                          tgtttATATTTTTTGTTTAAG‑GGGGG‑GG‑TTGGATTT‑TTTTC‑TC‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTatc                                                                                                                                                                           <  1:388947/93‑1 (MQ=255)                                                                                                                                                                          ccctttatttttttttttAAA‑CCGGG‑GGTTTAAATTT‑TTGTC‑TA‑CACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAACTATCCAGc                            <  1:1039799/231‑1 (MQ=255)                                                                                                                                                                             ttATTTTTTTTGTATAAT‑CGGGC‑GT‑TTGAATTG‑TTGTT‑TA‑CACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTaaaga                                                      <  1:1233331/209‑1 (MQ=255)                                                                                                                                                                             ttATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTG‑TTGTC‑TT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGGAATAAATCGAGTTGTTCCTGCGTTGTTGCCGCCCGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCatat                        >  1:87009/1‑239 (MQ=255)                                                                                                                                                                                  ttttttttTAAAA‑CGGGC‑GG‑TTGATTTG‑TTGTC‑TT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTatcatc                                                                                                                                                                        <  2:40438/91‑1 (MQ=255)                                                                                                                                                                                       tGTTTAAA‑CTGGC‑GG‑TTGAATTG‑TTGTT‑TT‑CAACCGCCCTGAGAAAATCACTCTGCCCACAGCGCCCGTG‑CCAAC‑GTTTTTTCATCATCCGTACGCTCGTTTA‑CCCCCGGGCTGGACAAAAACTGCTTTAACAAATCAGGTAATAAATCGAGTTTTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAAAGGGGGaaagaa                                                     >  1:711280/1‑200 (MQ=255)                                                                                                                                                                                       tGTTTAAA‑CTGGC‑GG‑TTGAATTG‑TTGTC‑TT‑CCACCGCCATGAGAAACTCACTCTGCCCCCAGCGCCCGTG‑CCCAC‑GGTTTATCATCATCCGTACGCCCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCCGTCCATTGAAAAAAGGGGGAAAGAAAGGCACATg                                            >  2:1233331/1‑209 (MQ=255)                                                                                                                                                                                       tGTTTAAA‑CTGGC‑GG‑TTGAATTG‑TTGTC‑TT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTTTCATCATCCGTACGCTCGTTTA‑CCCCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGGaaag                                                       >  1:399943/1‑198 (MQ=255)                                                                                                                                                                                       tGTTAAAA‑CTGGG‑GT‑TTAATTTG‑TTGTC‑TA‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGGG‑CCAAC‑GTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCTAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg              <  1:751730/239‑1 (MQ=255)                                                                                                                                                                                        ttttACA‑CTGGCGGG‑TTAATTTG‑TTTCC‑TT‑CACCGGCAATGAGAAACTCCCTCGCCCCACAGCGCCAGTG‑CCAAC‑GTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg              <  2:44551/238‑1 (MQ=255)                                                                                                                                                                                          ttAAA‑CTGGC‑GG‑TTGAATTG‑TTGTC‑TT‑CCACCCCCATTAGAAACTCACTCTTCCCCCCGCGCCAGTG‑CCCAC‑GTTTTATCATTCTCCCTACCCTCGTTTA‑CCCCCCGGCCGGACAAAAACTTCTTTAACAATTTTGGTAATAAATTCAGTTGTTCCTGCGTTTCTGCCGCCCGTCCCTTGAAAAACCGGGTAAAGAAAGGCACATgag                                          >  2:1122944/1‑208 (MQ=255)                                                                                                                                                                                          ttAAA‑CTGGC‑GG‑TTGAATTG‑TTGTC‑TT‑CAACCCCCATGAGAAACTCACTCTGCCCCCAGCGCCCGTG‑CCCAA‑GTTTTTTCATCATCCGTACGCCCGTTTA‑CCGCCGGGCTGGACAAAAACCGCTTTAACAAATCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAAAGGGGTAAAAAAAGGGAAAATAGGAGAATTTTCCAGCATATTTAACGCCAGta            >  1:906127/1‑237 (MQ=255)                                                                                                                                                                                          ttAAA‑CGGGG‑GG‑GTTAATTGTTTGTC‑TT‑AACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgag                                          <  1:769028/209‑1 (MQ=255)                                                                                                                                                                                            aaa‑CTGGC‑GG‑TTGAATTG‑TTGTC‑TT‑CAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCCGTG‑CCAAC‑GTTTTATCATCATCCGTACGc                                                                                                                                                              >  1:40438/1‑91 (MQ=255)                                                                                                                                                                                             aa‑CGGGC‑GT‑TTAATTTG‑TTGTT‑TT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTatcatc                                                                                                                                                                        <  1:1035915/80‑1 (MQ=255)                                                                                                                                                                                                    c‑GG‑TTGAATTG‑TTGTC‑TT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCCGTG‑CCAAC‑GTTTTTTCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAc   >  1:217998/1‑238 (MQ=255)                                                                                                                                                                                                    c‑GG‑TTGAATTG‑TTGTC‑TT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCCAA‑GTTTTATTATCATCCGTACGCCCGTTTA‑CCGCCGGGCTGGGCAAAAACTGCTTTAAAAATTCAGGGAATAAAACGAGTTGTTTCTGCGTTTCTGCCGCCAGTCCCTTGAAAAAAGGGGGAAAGAAAGGGCAATGAGGAGAATTATc                                >  2:769028/1‑209 (MQ=255)                                                                                                                                                                                                      gg‑TTAAATTG‑TTGTC‑TT‑AACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAcc  <  1:272659/238‑1 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       AAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAAT‑TAGTTCG‑CTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGC‑GG‑TTGAATTG‑TTGTC‑TT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTG‑CCAAC‑GTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACC  >  NC_000913/2151484‑2151914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑