Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,878,331 | 0 | T | G | 73.7% | 56.3 / 20.8 | 39 | I206R (ATA→AGA) | yeaP | diguanylate cyclase |
Reads supporting (aligned to +/- strand): ref base T (4/6); new base G (13/15); total (18/21) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
CCAGCAGTGAGAAGCGCCAGTGGAGTGAACGCGCGGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT‑CATTCAGGTTGGCGAGCGCCTTAAT‑ACGCTCCAGCAAAATGGCGAAG‑TTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAA > NC_000913/1878097‑1878563 | ccAGCAGTGAGAAGCGCCAGTGGAGTGAACGCGCGGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGCTTTTTGAAAATCTGACGCCACTGTTTTCCCT‑CTCCCGGCATCTTACCCATAAGATaaat > 2:1115516/1‑237 (MQ=255) ggAGTGAACGCGCGGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGAGAATGATCGCGTTTAt < 1:468645/229‑1 (MQ=255) tGAACGCGCGGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTTAAAATCTTACGACACTGTTTTCCCT‑CGCCCGGCCTCTTAACCCTAAGAGAATGATCGCGTTTTTCGCTCTTGATAACt > 2:560251/1‑239 (MQ=255) gTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGGGGTTTTTTAAAATCTTACGACACTGTTTTTCCTCCCCCCGGCGCCTT‑ACCACAAAAAAATGATCCCGGTTTTTGTTCCTGTTAACTTACAATTAATCAAtaa > 2:20972/1‑237 (MQ=255) aCAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGGTTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCCTCTTTAACATAAGAGAATGATCCCGGTTATCCATCTGGATAAATTCCAATTTAACAATTATCGtt > 2:143428/1‑239 (MQ=255) tATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGGAAATCTGACGACACTGTTTTCCCT‑CGCCCGGTCTGTTGACCAAGAGGTATTTAACGGGTTTGTCGATATGGAAAACTTCAAATTAATCAATCATCGtt < 2:415333/234‑1 (MQ=255) tATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGtt < 1:166932/234‑1 (MQ=255) tATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGAGAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGtttt < 1:143428/236‑1 (MQ=255) tATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGAGAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGt < 1:560251/239‑1 (MQ=255) tATTCGCCGGACTGATTGCAAAATTTATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATGGCGCAATCGTATACCGACTCGTTAACCGGGCTTCCGAAGCGGCAGGCTATTTTTGAAAATCTAACGACACTGTTGTCCCT‑CGCCCGGCATCTTAACCATAAGATAATGATCGAGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGt < 2:1181748/239‑1 (MQ=255) cGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACCCTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATTGATCTGGATAACTTCAAATTTATCAATGATCGTTTTGGTCataata > 1:1175822/1‑239 (MQ=255) aaTATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCTGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGAGAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGttt < 1:120380/239‑1 (MQ=255) atTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CCCCCGGCCTCTTAACCATAAGAGAATGATCGCGTTTTTCGATCTGGATAACTTTAAATTAATCAATGATCGTTTTGGTCATAATATGGGCGATATGttttct > 2:73330/1‑234 (MQ=255) tCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTTAAAAACTGACGAACCTGTTTTTCCT‑CCCCCGGCATCTTAACCAAAAAAGAATGATCGCGTTTATCGATCTGGGTAACTTCCAAATAAACAATGATCGTTTTTGGTATAATAGAGGCGa > 2:1160699/1‑225 (MQ=255) agGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCCGCATCTTAACCATAAGAGAATGATCGCGGTTATCGATCTGGGTAAATTCAAATTAATCAATGAACGTTTTGGTCATAATAATGGCGATCTGTTTTTCCTTTCAGGtggc > 2:1117459/1‑236 (MQ=255) aCTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGAGAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT‑CATTCAGGTTGGCGAg < 1:139929/238‑1 (MQ=255) gggTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTGTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGAGAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGtt < 1:446639/191‑1 (MQ=255) ttGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGAGAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT‑CATTCAGGTTGGCGAGCGCCt < 2:977595/238‑1 (MQ=255) gCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCGTCTTAACCATAAGAGAATGATCGCGTTTTTAGATCTGGGTTAATTCAAATTAATAAATGATTGTTTTGGGTataa > 2:446639/1‑192 (MQ=255) tGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTTCCT‑CGCCCGGCATCTTAACCATAAGAGAATGATCGCGTTTTTCGATCTGGATAACTTCCAATTAATCAATGATTGTTTTGGTCATAATAGTGGCGATCTGTTTTT‑CATTTAGGGTGGCGAGGGCCTTAAT‑ACGcc > 2:1145780/1‑237 (MQ=255) tGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGAGAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT‑CATTCAGGTTGGCGAGCGCCTTAAT‑ACGCTc < 2:535878/239‑1 (MQ=255) tgctgcGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGAGAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT‑CATTCAGGTTGGCGAGCGCCTTAAT‑ACGCTCCAGCAAAATGGCg < 1:1117459/239‑1 (MQ=255) tgctgcGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGAGAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT‑CATTCAGGTTGGCGAGCGCCTTAAT‑ACGCTCCAGCAAAATGGCg < 1:1145780/239‑1 (MQ=255) gcAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTTAAAATCTAACGACACTGTTTTCCCT‑CGCCCGGCATCTTTACCATAAGAGAATGATCGCGTTTTTCGAACTGGATAACTTCAACATAATCCATTATTGTTTTGGTCATAATAGTGGCGAACTGTTTTT‑CATTCCGGTTTGCGAGGGCCTTTAT‑ACGCTCCAGCAAAAAGGCGGAG‑TTTTTGgtccg > 2:131783/1‑236 (MQ=255) tCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGAGAATGATCGTGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT‑CATTCAGGTTGGCGAGCGCCTTAAT‑ACGCTCCAGCAAAATGGCGAAG‑TTATTGgt < 1:571082/231‑1 (MQ=255) taCCGACTCGTTAACCGGGCTACCGAATCGGCGGGGGATTTTTTAAAAACTGACGACACTGTTTTCCCT‑CGCCCGGAATCTTAACCAAAAGAGAATGATCGCGTTTATAGATCTGGGTAACCTTAAATTAATTAATGATTGGTTTGGTCATAATAATGGTGAGTTGTTTCT‑CATTAAGGTTGGGGAGGGGCTTCAT‑ACGCTCCAGGAAAATGGATAAG‑ATATTGgtcggttcgcggggg > 2:153503/1‑228 (MQ=255) aCTCGTTAACCGGGCTACCGAATCGGCGGGCGGTTTTTTAAAAACTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGAGAATGATCGGGTTTATCGATCTGGATAAATTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT‑CATTTAGGTTGGCGAGCGCCTTTAT‑ACGCCCCAGCAAAATGGCGAAA‑TTTTTGGTCGGTTcggtg > 2:571082/1‑229 (MQ=255) aCTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTTAAAAACTGACGACACCGTTTTTCCC‑CCCCCCGCCTCTTTACCATAAAAGAATGATCGCGTTTATCGCTCTTGGTAACTTCAAATTAATCAATGGTCCGTTTTGTCATAATAATGGCGGTTTGTTTTT‑CATTCCTGGTTGCGGACGCCTTTATAACGCCCCA‑CCAAATGGTGGAGTTTTTTGGTGGTCCCGGGGGtggggg > 2:1152102/1‑235 (MQ=255) aCTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGAGAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT‑CATTCAGGTTGGCGAGCGCCt > 2:562583/1‑187 (MQ=255) aCTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGAGAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT‑CATTCAGGTTGGCGAGCGCCt < 1:562583/187‑1 (MQ=255) ggCTACCGAACCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAAt > 1:942868/1‑111 (MQ=255) ggCTACCGAACCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAAt < 2:942868/111‑1 (MQ=255) cGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT‑CATTCAGGTTGGCGAGCGCCTTAAT‑ACGCTCCAGCAAAATGGCGAAG‑TTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTa < 2:557502/239‑1 (MQ=255) ggcgggcgATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT‑CATTCAGGTTGGCGAGCGCCTTAAT‑ACGCTCCAGCAAAATGGCGAAG‑TTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAaacaac < 2:230605/239‑1 (MQ=255) t‑CGCCCGGCATCTTAACCATAAGAGAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT‑CATTCAGGTTGGCGAGCGCCTTAAT‑ACGCTCCAGCAAAATGGCGAAG‑TTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTcag < 1:979650/239‑1 (MQ=255) ccGGCATCTTAACCATAAGAGAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT‑CATTCAGGTTGGCGAGCGCCTTAAT‑ACGCTCCAGCAAAATGGCGAAG‑TTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTg < 1:991417/220‑1 (MQ=255) tAACCATAAGAGAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT‑CATTCAGGTTGGCGAGCGCCTTAAT‑ACGCTCCAGCAAAATGGCGAAG‑TTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTg < 2:94660/239‑1 (MQ=255) aaCCATAAGAGAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT‑CATTCAGGTTGGCGAGCGCCTTAAT‑ACGCTCCAGCAAAATGGCGAAG‑TTATTGGTCGTCTCGGCGGTGATGAGGTTTTAGTTGTTTTACTAAAAAACGAGAATGCGGATATTTTGTTGCTGCGAGAAAGCa > 2:991417/1‑220 (MQ=255) aTAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT‑CATTCAGGTTGGCGAGCGCCTTAAT‑ACGCTCCAGCAAAATGGCGAAG‑TTATTGGTCGTCTCGGCGGTGGTGGGGTTTTTGTTTTTTTACTAAAAAAACAGAAATGGGAGATTTTGGTGCTGGGGGGACGGATTTCACAGCAAAAACGGGGGGaa > 2:1019300/1‑239 (MQ=255) | CCAGCAGTGAGAAGCGCCAGTGGAGTGAACGCGCGGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCT‑CGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCT‑CATTCAGGTTGGCGAGCGCCTTAAT‑ACGCTCCAGCAAAATGGCGAAG‑TTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAA > NC_000913/1878097‑1878563 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |