Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,175,124	A→C	L91L (CTT→CTG)	parE ←	DNA topoisomerase IV, subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,175,124	0	A	C	100.0%	135.8 / NA	38	L91L (CTT→CTG)	parE	DNA topoisomerase IV, subunit B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (38/0);  total (38/0)

GCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCAAAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGTGGTATCGGTAT  >  NC_000913/3174892‑3175320                                                                                                                                                                                                                                         |                                                                                                                                                                                                     gCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCCCGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGCAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATc                                                                                                                                                                                                >  1:408827/1‑239 (MQ=255) gCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATc                                                                                                                                                                                                >  1:393174/1‑239 (MQ=255)      aaGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTc                                                                                                                                                                                           >  1:850447/1‑239 (MQ=255)                  gTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTac                                                                                                                                                                               >  1:541412/1‑239 (MQ=255)                   tACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTCATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTac                                                                                                                                                                               >  1:646848/1‑238 (MQ=255)                        gTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGAACAGAATCAGTTCAACCGCCGGTacac                                                                                                                                                                             >  1:181829/1‑235 (MQ=255)                          aTTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCctct                                                                                                                                                                        >  1:370886/1‑238 (MQ=255)                            tGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTcc                                                                                                                                                                     >  1:59744/1‑239 (MQ=255)                              cGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCgg                                                                                                                                                                   >  1:993515/1‑239 (MQ=255)                                   aCCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGaa                                                                                                                                                              >  1:916906/1‑239 (MQ=255)                                   aCCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGaa                                                                                                                                                              >  1:288554/1‑239 (MQ=255)                                                                gCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCTCCGGTACACCCTCTTCCGGGTGAata                                                                                                                                                            >  1:906762/1‑212 (MQ=255)                                                                  aCCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAAt                                                                                                                               >  1:725022/1‑239 (MQ=255)                                                                      tttCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACt                                                                                                                           >  1:382505/1‑239 (MQ=255)                                                                                                 aaaCCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTaaaa                                                                                                  >  1:259184/1‑237 (MQ=255)                                                                                                     cTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGACTATCCACCGGCATCCCGCGCCCATCGCCACTACCTTCTAACGACTGTTCAGCATGTAAAATAACg                                                                                             >  2:1057757/1‑238 (MQ=255)                                                                                                       gACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACAccc                                                                                                                                                                           >  1:786534/1‑158 (MQ=255)                                                                                                       gACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTaaaa                                                                                                  >  1:273026/1‑231 (MQ=255)                                                                                                       gACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTACAATAACGTcc                                                                                          >  2:684324/1‑239 (MQ=255)                                                                                                       gACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTcc                                                                                          >  2:1132007/1‑239 (MQ=255)                                                                                                        aCCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTcc                                                                                          >  1:273610/1‑238 (MQ=255)                                                                                                             cgcgGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGt                                                                                    >  1:661136/1‑239 (MQ=255)                                                                                                                      cGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCCATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTg                                                                            >  1:595561/1‑238 (MQ=255)                                                                                                                                 aCGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGt                                                                  >  1:531305/1‑237 (MQ=255)                                                                                                                                 aCGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGt                                                                  >  2:630082/1‑237 (MQ=255)                                                                                                                                       ttCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCAt                                                           >  1:1132720/1‑238 (MQ=255)                                                                                                                                         cGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATcc                                                         >  2:483988/1‑238 (MQ=255)                                                                                                                                                 cGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTa                                                >  2:840966/1‑239 (MQ=255)                                                                                                                                                      accaccGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAAt                                           >  1:882535/1‑239 (MQ=255)                                                                                                                                                                     aCGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTaa                                                                                                    >  1:457398/1‑167 (MQ=255)                                                                                                                                                                                 ccgccAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACTTCCACGCGTTTTGCGCGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCg                >  1:560320/1‑239 (MQ=255)                                                                                                                                                                                     cAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAggg                  >  1:633126/1‑233 (MQ=255)                                                                                                                                                                                     cAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGTg            >  1:923239/1‑239 (MQ=255)                                                                                                                                                                                              ggTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGTGGTATCGGt    >  1:149995/1‑238 (MQ=255)                                                                                                                                                                                               gTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCa                                             >  1:789332/1‑196 (MQ=255)                                                                                                                                                                                               gTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGTGGTATCGGtat  >  1:1002544/1‑239 (MQ=255)                                                                                                                                                                                                                            catgcaGACGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGa                                         >  1:649236/1‑171 (MQ=255)                                                                                                                                                                                                                                   aCGGCACAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCcaca                                                      >  1:48849/1‑151 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                     GCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTTTTCGCCATTTTCAAAGGCGATGTTATAAACCTGACCATCGCGGCGCACGTTAACTTCTACGCGCTTCGACAGGGCGTTAACCACCGAAATCCCCACGCCATGCAGGCCGCCAGAGAACTGGTAATTTTTGTTAGAGAATTTACCGCCTGCATGCAGACGGCAAAGAATCAGTTCAACCGCCGGTACACCCTCTTCCGGGTGAATATCCACCGGCATCCCGCGCCCATCGTCAATAACTTCTAACGACTGGTCAGCATGTAAAATAACGTCCACGCGTTTTGCGTGACCCGCCAGTGCTTCATCCACACTGTTATCAATGACTTCTTGCCCCAAATGGTTAGGGCGAGTGGTATCGGTAT  >  NC_000913/3174892‑3175320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑