Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,336,051 A→C D148E (GAT→GAG murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,336,0510AC100.0% 269.7 / NA 79D148E (GAT→GAGmurAUDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (39/40);  total (39/40)

TAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATC  >  NC_000913/3335818‑3336278
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                   
tAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGAc                                                                                                                                                                                                                                >  2:1112373/1‑239 (MQ=255)
          cgccTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACgg                                                                                                                                                                                                                              <  2:75646/231‑1 (MQ=255)
          cgccTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGa                                                                                                                                                                                                                             <  1:1112373/232‑1 (MQ=255)
            ccTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAAc                                                                                                                                                                                                                    >  2:504711/1‑239 (MQ=255)
            ccTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAAc                                                                                                                                                                                                                    >  1:423452/1‑239 (MQ=255)
              tAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGt                                                                                                                                                                                                                  <  1:1131213/239‑1 (MQ=255)
                        acacCGTCGATGACGATAAGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTGCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAAGCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCttcttc                                                                                                                                                                                                        <  2:345881/239‑1 (MQ=255)
                            cTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGt                                                                                                                                                                                                    >  2:137807/1‑239 (MQ=255)
                            cTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGt                                                                                                                                                                                                    >  1:799510/1‑239 (MQ=255)
                            cTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGt                                                                                                                                                                                                    >  1:473809/1‑239 (MQ=255)
                            cTTCGATGACGATACGATCGGTGCCCTGACCACTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGt                                                                                                                                                                                                    >  2:284602/1‑239 (MQ=255)
                                     cgatacgatCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTc                                                                                                                                                                                           <  2:473809/239‑1 (MQ=255)
                                        tacgatCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACCTGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTcgc                                                                                                                                                                                         >  1:1184704/1‑238 (MQ=255)
                                               ggTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCtt                                                                                                                                                                                                                        >  2:41712/1‑200 (MQ=255)
                                               ggTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCtt                                                                                                                                                                                                                        <  1:41712/200‑1 (MQ=255)
                                                     cTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGa                                                                                                                                                                           <  1:1156247/239‑1 (MQ=255)
                                                           gCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAg                                                                                                                                                                     >  1:339383/1‑239 (MQ=255)
                                                             tAATTTTCGCCCCCAGCGTAATCAGGAAGTTCGGGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGc                                                                                                                                                                        <  1:75030/234‑1 (MQ=255)
                                                             tAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGaa                                                                                                                                                                   <  1:994295/239‑1 (MQ=255)
                                                                      cACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGa                                                                                                                                                          <  1:699004/239‑1 (MQ=255)
                                                                              gTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAAc                                                                                                                                                     <  2:1081497/236‑1 (MQ=255)
                                                                                       aaGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGcccc                                                                                                                                         >  2:303355/1‑239 (MQ=255)
                                                                                          ttCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGa                                                                                                                                       >  2:888994/1‑238 (MQ=255)
                                                                                                 tATCGACGTTTCCCGGTTCCCCGCCGTCTTTTTAAAAAACCTTGGTCCCTCCCGCCAGGGTTGCAGCACACATGATGGCCCCCTTTGCCCCAACGCTGACTTTACCCATCACGATATGTCCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTACCCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAAAAATGTTTAGATCAACCGGCCGCGCACCGATCGTAc                                                                                                                                 <  2:1144838/237‑1 (MQ=255)
                                                                                                    cgacgaTTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCg                                                                                                                            <  1:137807/239‑1 (MQ=255)
                                                                                                           ttCCGTTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTa                                                                                                                     <  1:303355/239‑1 (MQ=255)
                                                                                                           ttCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAgg                                                                                                                       >  1:1144838/1‑237 (MQ=255)
                                                                                                           ttCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGACCGTACAACCGCCAgcaa                                                                                                                     >  1:55130/1‑236 (MQ=255)
                                                                                                                                     ataatCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGaa                                                                                                                                                                   <  1:286982/168‑1 (MQ=255)
                                                                                                                                     ataatCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGaa                                                                                                                                                                   >  2:286982/1‑168 (MQ=255)
                                                                                                                                         tCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCt                                                                                        >  1:490214/1‑239 (MQ=255)
                                                                                                                                              gtgCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAgg                                                                                                                       >  2:939095/1‑203 (MQ=255)
                                                                                                                                              gtgCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAgg                                                                                                                       <  1:939095/203‑1 (MQ=255)
                                                                                                                                                      cGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTAc                                                                                                                                 <  2:415671/185‑1 (MQ=255)
                                                                                                                                                      cGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTAc                                                                                                                                 >  1:415671/1‑185 (MQ=255)
                                                                                                                                                                gcagcaCACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAAc                                                                                                                                                     <  2:165256/155‑1 (MQ=255)
                                                                                                                                                                gcagcaCACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAAc                                                                                                                                                     >  1:165256/1‑155 (MQ=255)
                                                                                                                                                                 cagcaCACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACgg                                                                                                                                                                                                                              >  1:655875/1‑81 (MQ=255)
                                                                                                                                                                 cagcaCACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACgg                                                                                                                                                                                                                              <  2:655875/81‑1 (MQ=255)
                                                                                                                                                                     acacaTGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAg                                                            <  2:938156/239‑1 (MQ=255)
                                                                                                                                                                        caTGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGt                                                                                                                                                                                                                  >  2:1173334/1‑86 (MQ=255)
                                                                                                                                                                        caTGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGt                                                                                                                                                                                                                  <  1:1173334/86‑1 (MQ=255)
                                                                                                                                                                           gatggccgcgattGCGCCAGCACTGACTGTATCGCTCACTATATGTGCACCTTTCAAACGAGCCTCGACTAAGGCTTTAACGTAACCTTCTTCCATGTTGATGGGCGCGCCTAAGTGTTCGAGGCCCGAAATGTGTAGATCCGCCGGACGCGCCCCGATCGTACAACCGACAGGTAGTGAAATTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAACCACGCa                                                      <  2:90163/228‑1 (MQ=255)
                                                                                                                                                                             tgGTCCCCGTTGCCCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGAAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCAt                                                     <  1:856454/238‑1 (MQ=255)
                                                                                                                                                                              ggTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATgg                                                   >  2:1104317/1‑239 (MQ=255)
                                                                                                                                                                                       tGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGcc                                                                      <  2:212826/211‑1 (MQ=255)
                                                                                                                                                                                       tGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGcc                                                                      >  1:212826/1‑211 (MQ=255)
                                                                                                                                                                                         cgcCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGa                                        >  1:42318/1‑239 (MQ=255)
                                                                                                                                                                                            cAACGCTGACTTTATCCATCACGATATGTCCGCCTTTCAACCGACCCTCGACGGAGGGTTTAACGTATCCTTCTTCCATTTTGATGGTCGCGACTAATTGTTCGAGGCCAGAAATGTGTAGATCATCCGGACGCGCACCGATCGTCCAACCGCCAGGTAGTGAAAAGTGCCCCTGACGAAAGCGCGCGACCGGCGGCCGCGGCGGCCGGATAGAAGGAAGCATGGTTTTATTCAGAtg                                      <  2:315718/238‑2 (MQ=255)
                                                                                                                                                                                              aCGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGt                                                                                                                                                                                                                  >  2:347238/1‑64 (MQ=255)
                                                                                                                                                                                              aCGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGt                                                                                                                                                                                                                  <  1:347238/64‑1 (MQ=255)
                                                                                                                                                                                              aCGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTg                                                                                                                  <  1:871527/160‑1 (MQ=255)
                                                                                                                                                                                              aCGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTg                                                                                                                  >  2:871527/1‑160 (MQ=255)
                                                                                                                                                                                              aCGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGCGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTa                                   >  1:194543/1‑239 (MQ=255)
                                                                                                                                                                                                gCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAg                                 <  2:42318/239‑1 (MQ=255)
                                                                                                                                                                                                  tGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGt                               <  2:339383/239‑1 (MQ=255)
                                                                                                                                                                                                    aCTTTATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTgc                             >  1:816960/1‑239 (MQ=255)
                                                                                                                                                                                                    aCTTTATCCATCACGATATGTGCAACTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGGGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCACTGACCAAAGCGCGCTACCAGCGGCCCCACCGCCCCGATAGAAGCACGCAAGGCTTTAACAAGTCCGTAAGGTgc                             >  2:189386/1‑239 (MQ=255)
                                                                                                                                                                                                       ttATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCa                          <  2:490214/239‑1 (MQ=255)
                                                                                                                                                                                                        tATCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGCCGCGCCCCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAg                         <  1:284602/239‑1 (MQ=255)
                                                                                                                                                                                                          tCCATCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGaa                       <  1:888994/239‑1 (MQ=255)
                                                                                                                                                                                                              tCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGAt                                                                 <  2:1059018/193‑1 (MQ=255)
                                                                                                                                                                                                              tCACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGAt                                                                 >  1:1059018/1‑193 (MQ=255)
                                                                                                                                                                                                               cACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAAcc                                           >  2:151941/1‑214 (MQ=255)
                                                                                                                                                                                                               cACGATATGTGCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAAcc                                           <  1:151941/214‑1 (MQ=255)
                                                                                                                                                                                                                       gtgCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGCAAGGAGCGCAGAATACATTAACGta           >  2:183609/1‑237 (MQ=255)
                                                                                                                                                                                                                        tgCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTaa                                                                                                                                                                                                                >  2:1162867/1‑40 (MQ=255)
                                                                                                                                                                                                                        tgCACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTaa                                                                                                                                                                                                                <  1:1162867/40‑1 (MQ=255)
                                                                                                                                                                                                                          cACCTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCACCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCgg       >  2:818553/1‑239 (MQ=255)
                                                                                                                                                                                                                            ccTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACgcgc                                                                                                                                            <  2:432665/104‑1 (MQ=255)
                                                                                                                                                                                                                            ccTTTCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACgcgc                                                                                                                                            >  1:432665/1‑104 (MQ=255)
                                                                                                                                                                                                                             cTTTCAAACGACCCTCGCGGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCa    <  2:423452/239‑1 (MQ=255)
                                                                                                                                                                                                                              tttCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAAtg                                                                                                                                                                >  2:989969/1‑82 (MQ=255)
                                                                                                                                                                                                                              tttCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAAtg                                                                                                                                                                <  1:989969/82‑1 (MQ=255)
                                                                                                                                                                                                                              tttCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTg                                                                                                                  <  2:990808/128‑1 (MQ=255)
                                                                                                                                                                                                                              tttCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTg                                                                                                                  >  1:990808/1‑128 (MQ=255)
                                                                                                                                                                                                                               ttCAAACGACCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATc  >  2:684546/1‑239 (MQ=255)
                                                                                                                                                                                                                                       aCCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGAcgc                                                                                                                                              <  1:190498/91‑1 (MQ=255)
                                                                                                                                                                                                                                       aCCCTCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGAcgc                                                                                                                                              >  2:190498/1‑91 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                   
TAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACGATATGTGCACCTTTCAAACGACCATCGACGGAAGCTTTAACGTAACCTTCTTCCAGTTTGATGGTCGCGCCTAATTGTTCGAGGCCAGAAATGTGTAGATCAACCGGACGCGCACCGATCGTACAACCGCCAGGTAGTGAAACTTGCCCCTGACCAAAGCGCGCTACCAGCGGCCCCAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATC  >  NC_000913/3335818‑3336278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: