Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,341,805	A→C	K180N (AAA→AAC)	kdsD →	D‑arabinose 5‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,341,805	0	A	C	100.0%	266.5 / NA	70	K180N (AAA→AAC)	kdsD	D‑arabinose 5‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (38/32);  total (38/32)

TCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAAT‑TCCAGTGCTTAAGCGTCTTCA‑CGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATGGCGC‑GCGCCGCAGATGTGCATCTGTGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAGGCATCTTTACCGACG  >  NC_000913/3341573‑3342031                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                   tctcTAACTCGGGTGATCCCAGCGAAATCCCGGCCTTAAT‑CCCAGTGCTTAGGCGTCTTCA‑CGTCCCGTTAATCGGCATCACGGGTCGCCCGGAGGGCAGCAGGGCGC‑GGGCCGAAGATGTGCATCGGGGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAacgcacgc                                                                                                                                                                                                                              <  2:823014/239‑1 (MQ=255)   tctAACTCTGGTGAATCCAGCGAAATCACGGCCTTAAT‑TCCCGTGCTTAAGCGTATACG‑CGTCCCGTTAATCTGCATCCCCGGTGGCCGGGAGAGCAGCAGGGCGC‑GCGCCGCAGATGTGCATCTGTGTGTTAAA‑GTGGCAAAGGAGGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCgg                                                                                                                                                                                                                            <  2:426741/239‑1 (MQ=255)           tGGTGAATCCAGCGAAATCACGGCCTTAAT‑TCCAGTGCTTAAGCGTCTTCA‑CGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATGGCGC‑GCGCCGCAGATGTGCATCTGTGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCttt                                                                                                                                                                                                                        >  1:319649/1‑235 (MQ=255)             gcgAATCCAGCGAAATCACGGCCTTAAT‑TCCAGTGCTTAAGCGTCTTCC‑CGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATGGCGC‑GCGCCGCAGATGTGCCTCTGTGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTAct                                                                                                                                                                                                                     >  1:75873/3‑236 (MQ=255)             gTGAATCCAGCGAAATCACGGCCTTAAT‑TCCAGTGCTTAAGCGTCTTCA‑CGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATGGCGC‑GCGCCGCAGATGTGCATCTGTGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGAACGCGGCTTTAct                                                                                                                                                                                                                     >  1:232166/1‑236 (MQ=255)                       cGAAATCACGGCCTTAAT‑TCCAGTGCTTAAGCGTCTTCA‑CGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATGGCGC‑GCGCCGCAGATGTGCATCTGTGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGAtttt                                                                                                                                                                                                         >  1:968514/1‑238 (MQ=255)                           aTCCCGGCTTTAAT‑CCCAGTGCTTAGGCGTCTTCA‑GGTCCCGTTAATCGGCATCCCCGGTCGCCGGGGGGGCAGCATGGCGC‑GCGCCGAAGATGGGCATCTGTGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGTTGGCACCGACCAGCAGCCCCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGct                                                                                                                                                                                                    <  2:1164483/239‑1 (MQ=255)                               cGGCCTTAAT‑GCCAGTGCTTAAGCGTCTTCA‑CGTACCGTTAATCTGCATCGCCGGTCGCCCGGAGAGCAGCATGGCGC‑GCGCCGCAGATGTGCATCTGGGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTca                                                                                                                                                                                                <  1:659841/239‑1 (MQ=255)                                tgCCTTAATCTCCGGT‑CTTAAGGGTTGTCA‑CGGTCCGTTAATCTGCTTCGCCGGTCGCCCGGGGAGCAGAATGGCGG‑CCGCCGCAGAGGTGCCTCTTTGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTAAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTcac                                                                                                                                                                                               <  2:200446/238‑1 (MQ=255)                                      aaT‑TCCAGTGCTTAAGCGTCTTCA‑CGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATGGCGC‑GCGCCGCAGATGTGCATCTGTGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAg                                                                                                                                                                                         >  1:272228/1‑239 (MQ=255)                                          gccAGTCCTTAGGCGT‑TTCACCGTCCCGTAAATCTCCACCCCCGGCCGCCGGAGGAGCAGCATGGCGC‑GGGCCGCAGATGTGCTTTTGTGTGTTAAA‑GTAGCGAAAGAGGCCTGCCCGTTGGGGCTGGCCCCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAggcg                                                                                                                                                                                      <  2:319649/238‑1 (MQ=255)                                                gCTTAAGCGTCTTCA‑CGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATGGCGC‑GCGCCGCAGATGTGCATCTGTGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCa                                                                                                                                                                                 <  1:15367/238‑1 (MQ=255)                                                         tCTTCA‑CGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATGGCGC‑GCGCCGCAGATGTGCATCTGTGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTa                                                                                                                                                                       >  2:258946/1‑239 (MQ=255)                                                                              gacaccccGGTCGCCCCGGGAGGCGCCATGCGCGGCCCCGCAGTTGGCCTTTTGTGTTTAAAA‑GTAGAGAAAGAACCCGGTCCGTGAGGGCGGGCCCGGACCAGCAGCCCCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTaaa                                                                                                                                                    <  2:75873/233‑1 (MQ=255)                                                                               cATCACCGGTCGCCCGGAGAGCAGCATGGCGC‑GCGCCGCAGATGTGCATCTGTGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACg                                                                                                                                                  >  1:1155403/1‑239 (MQ=255)                                                                               cATCACCGGTCGCCCGGAGAGCAGCATGGCGC‑GCGCCGCAGATGTGCATCTGTGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACg                                                                                                                                                  >  2:329400/1‑239 (MQ=255)                                                                                  cACCGGTCGCCCGGAGAGCAGCATGGCGC‑GCGCCGCAGATGTGCATCTGTGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGata                                                                                                                                               <  1:258946/239‑1 (MQ=255)                                                                                                         tGGCGC‑GCGCCGCAGATGTGCATCTGTGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGTTATTATGCATACGGGCGAGGAGAt                                                                                                                         >  1:1035817/1‑238 (MQ=255)                                                                                                            cgc‑gcgcCGCAGATGTGCATCTGTGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCa                                                                                                                                        >  1:193530/1‑220 (MQ=255)                                                                                                                 cgccgcAGATGTGCATCTGTGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCg                                                                                                                     >  1:726750/1‑235 (MQ=255)                                                                                                                      cAGATGTGCATCTGTGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACAccc                                                                                                                                                                                           >  2:912648/1‑160 (MQ=255)                                                                                                                      cAGATGTGCATCTGTGTGTTAAA‑GTAGCAAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACAccc                                                                                                                                                                                           <  1:912648/160‑1 (MQ=255)                                                                                                                               tttggggtgtTAAA‑GTAGGAAAAGAAGCCTGTCCGTTAGGGCTGGCACCGCCCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGc                                                                                                    <  2:594026/232‑1 (MQ=255)                                                                                                                                   gtgtgtTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGAtatt                                                                                                                                             >  2:962457/1‑193 (MQ=255)                                                                                                                                   gtgtgtTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACgg                                                                                                     >  1:325074/1‑233 (MQ=255)                                                                                                                                   gtgtgtTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTc                                                                                               >  1:657426/1‑239 (MQ=255)                                                                                                                                   gtgtgtTAAA‑GGAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACgg                                                                                                     >  1:1179070/1‑233 (MQ=255)                                                                                                                                   gtgtgtAAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGAtatt                                                                                                                                             <  1:962457/193‑1 (MQ=255)                                                                                                                                    tgtgtTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCCCCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTg                                                                                                                                                                                                        <  1:53103/133‑1 (MQ=255)                                                                                                                                    tgtgtTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTg                                                                                                                                                                                                        >  2:53103/1‑133 (MQ=255)                                                                                                                                    tgtgtTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGTGATGAGATCCCGCATGTTAAGAAAACGGCCat                                                                                                 >  1:594026/1‑235 (MQ=255)                                                                                                                                     tgtttAAAGGTAGCAAAAGAAGCCTGTCCGTTGGGGCTGGCCCCGACCAGCAGCCCCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACggg                                                                                                                                  <  2:399479/200‑1 (MQ=255)                                                                                                                                      ggtaaaa‑GTAGCAAAAGAAGCCTGTCCGTTAGGGCGGGCACCAACCAGCAGCCCCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACggg                                                                                                                                  <  2:807235/197‑1 (MQ=255)                                                                                                                                               tAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGTTGCGCGTAAACGATATTATGCATACGGGCGATGAGATc                                                                                                                        >  1:399479/1‑203 (MQ=255)                                                                                                                                                 gCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATc                                                                                                                        >  1:807235/1‑201 (MQ=255)                                                                                                                                                           ccTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGACTGCGTGACGCGTTGCTGGAAGtt                                                                        >  1:787572/1‑239 (MQ=255)                                                                                                                                                                         cTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGata                                                                                                                                               <  2:638974/154‑1 (MQ=255)                                                                                                                                                                         cTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGata                                                                                                                                               >  1:638974/1‑154 (MQ=255)                                                                                                                                                                           ggCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGGGACGCGTTGCTGGTAGTTACCCGCAAAAATCTTg                                                        >  1:238609/1‑239 (MQ=255)                                                                                                                                                                            gCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTgg                                                       >  1:763172/1‑239 (MQ=255)                                                                                                                                                                                  aCCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATg                                                   >  1:77484/1‑237 (MQ=255)                                                                                                                                                                                       cagcaCCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATcc                                                                                                                       >  1:836658/1‑164 (MQ=255)                                                                                                                                                                                       cagcaCCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATcc                                                                                                                       <  2:836658/164‑1 (MQ=255)                                                                                                                                                                                            ccaccGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGc                                       >  1:462766/1‑239 (MQ=255)                                                                                                                                                                                               cctccACGCTGGTTATGGGCGATGCCCTCGCTGTCGGGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACCCCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGAt                                    <  1:1147092/236‑1 (MQ=255)                                                                                                                                                                                                cGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATg                                   >  1:984797/1‑239 (MQ=255)                                                                                                                                                                                                   cACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTATTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCTTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGAt                                    <  1:888325/235‑1 (MQ=255)                                                                                                                                                                                                    aCGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCAt                                                                                                                  <  2:262480/156‑1 (MQ=255)                                                                                                                                                                                                    aCGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCAt                                                                                                                  >  1:262480/1‑156 (MQ=255)                                                                                                                                                                                                     cGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAat                              >  2:48877/1‑239 (MQ=255)                                                                                                                                                                                                        tGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAatat                            <  2:272228/238‑1 (MQ=255)                                                                                                                                                                                                         ggTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTAACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATatga                          <  2:628201/239‑1 (MQ=255)                                                                                                                                                                                                            tATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACg                                                                                                                                    >  2:461505/1‑130 (MQ=255)                                                                                                                                                                                                            tATGGGCGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACg                                                                                                                                    <  1:461505/130‑1 (MQ=255)                                                                                                                                                                                                                  cGATGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGaa                  <  1:329400/238‑1 (MQ=255)                                                                                                                                                                                                                   gATGCCCTCCGTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAgg                <  2:25408/239‑1 (MQ=255)                                                                                                                                                                                                                    aTGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCa                                                                                                                                                                                 <  2:715797/77‑1 (MQ=255)                                                                                                                                                                                                                    aTGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCa                                                                                                                                                                                 >  1:715797/1‑77 (MQ=255)                                                                                                                                                                                                                    aTGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGAtatt                                                                                                                                             >  2:353536/1‑113 (MQ=255)                                                                                                                                                                                                                    aTGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGAtatt                                                                                                                                             <  1:353536/113‑1 (MQ=255)                                                                                                                                                                                                                    aTGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACg                                                                                                                                    >  1:663868/1‑122 (MQ=255)                                                                                                                                                                                                                    aTGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACg                                                                                                                                    <  2:663868/122‑1 (MQ=255)                                                                                                                                                                                                                    aTGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGt                                                      <  1:614275/200‑1 (MQ=255)                                                                                                                                                                                                                    aTGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGt                                                      >  2:614275/1‑200 (MQ=255)                                                                                                                                                                                                                    aTGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAGGc               >  1:519005/1‑239 (MQ=255)                                                                                                                                                                                                                    aTGCCCTCGCTGTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAGGc               >  1:659652/1‑239 (MQ=255)                                                                                                                                                                                                                               gTCGCGCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAGTCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAGGCATCTTTACCGa    <  1:787265/239‑1 (MQ=255)                                                                                                                                                                                                                                 cgcgCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAGGCATCTTTACCGACg  <  2:462766/239‑1 (MQ=255)                                                                                                                                                                                                                                 cgcgCTGTTAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAGGCATCTTTACCGACg  <  2:325074/239‑1 (MQ=255)                                                                                                                                                                                                                                        ttAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTaaa                                                                                                                                                    <  1:422750/86‑1 (MQ=255)                                                                                                                                                                                                                                        ttAAACGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTaaa                                                                                                                                                    >  2:422750/1‑86 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                   TCTCTAACTCTGGTGAATCCAGCGAAATCACGGCCTTAAT‑TCCAGTGCTTAAGCGTCTTCA‑CGTACCGTTAATCTGCATCACCGGTCGCCCGGAGAGCAGCATGGCGC‑GCGCCGCAGATGTGCATCTGTGTGTTAAA‑GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTGCTGCGCGTAAACGATATTATGCATACGGGCGATGAGATCCCGCATGTTAAGAAAACGGCCAGTCTGCGTGACGCGTTGCTGGAAGTTACCCGCAAAAATCTTGGTATGACTGTCATTTGCGATGACAATATGATGATTGAAGGCATCTTTACCGACG  >  NC_000913/3341573‑3342031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑